• Food Science and Biotechnology
• /
• 제17권2호
• /
• pp.295-301
• /
• 2008

In this study, the antioxidant activities of red pepper seed kimchi extracts were examined. The extracts were evaluated by various antioxidant assays that included determinations of total phenolic and flavonoid contents, DPPH radical scavenging, superoxide anion radical scavenging, nitric oxide scavenging, superoxide dismutase (SOD) activity, metal chelating activity, and reducing power. All the kimchi extracts showed strong antioxidant activities by the tested methods. The 7% red pepper seed kimchi that was fermented for 6 days possessed the highest activity according to the performed tests. Yet, the highest scavenging activity was exhibited against nitrite, by extracts from kimchi for 0 day of fermentation rather than 6 days. All the activities for the kimchi fermented for 0 day with the addition of 7% red pepper seed increased markedly with an increase in concentration. With the exception of metal chelating and SOD activities, for the antioxidant properties assayed, the red pepper seed kimchi extracts were found to be highly effective.

• 한국약용작물학회지
• /
• 제21권4호
• /
• pp.255-261
• /
• 2013

The purpose of this study was to examined the antioxidant activities by water and 70% ethanol extract from durian (Durio zibethinus.) seed, sarcocarp and peel. Durian extract were studied for reducing sugar content, polyphenol content, superoxide dismutase (SOD) like activity, electron donating ability, nitrite scavenging ability, flavonoid content, hydroxy radical scavenging activity. Reducing sugar content were increased peel > sarcocarp > seed. Total polyphenol, flavonoid content, DPPH radical scavenging ability and SOD like activity were increased seed > peel > sarcocarp. Total polyphenol content was relatively high as $21.90{\pm}0.50mg/g$ in the ethanol extract of the seed. DPPH radical scavenging ability was relatively high as $62.08{\pm}2.63%$ in the water extract of the seed. Nitrite scavenging ability was no significant difference. Hydroxy radical scavenging activity was increased seed > peel > sarcocarp, was relatively high as $58.27{\pm}1.13%$ in the water extract of the seed.

• Advances in Traditional Medicine
• /
• 제9권2호
• /
• pp.128-134
• /
• 2009

The antioxidant potency of methanolic extract Clerodendrum infortunatum Linn. (MECI), which are widely used in the Indian indigenous system of medicine for different purposes, was studied. The antioxidant potential was evaluated using different established in vitro antioxidant tests viz. determination of total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, nitric oxide scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging and reductive power assay. It was found that MECI contain a high amount of polyphenolics and possesses significant free radical scavenging activity in all the assay. The higher activity was may be due to presence of richest amount of polyphenolics and flavonoids in it.

• 한국식품저장유통학회지
• /
• 제13권3호
• /
• pp.413-419
• /
• 2006

뜰보리수 열매의 식품영양학적 평가의 일환으로 추출용매별, 추출농도별에 따른 전자공여능, linolenic acid system을 이용한 항산화활성, SOD유사활성, superoxide anion radical 소거능, 아질산염 소거능 등을 측정하였다. 전자공여능은 물 추출물에서는 낮은 농도에서 비교적 높은 활성을 나타내었고, 에탄올과 메탄올 추출물의 농도가 증가할수록 항산화 활성이 증가하였으며, 메탄올 추출물에서 가장 높은 항산화 효과를 보였다. Linoleic acid system에 의한 항산화 활성은 추출물의 농도가 증가할수록 항산화 효과 또한 높아졌고, 물 추출물 보다는 에탄올 추출물과 메탄올 추출물에서 효과가 더 좋았다. SOD 유사활성은 추출물의 농도가 높을수록 SOD 활성도 높게 나타났으며, 메탄올 추출물에서 추출물 농도 2.0 mg/mL에서 가장 높은 활성을 타나내었다. Superoxide anion radical에 대한 저해정도는 물 추출물의 경우 추출물의 첨가농도가 낮을 때 높은 저해효과를 나타내었고, 에탄올과 메탄올 추출물에서는 추출물 첨가농도가 증가할수록 저해효과가 높았다. 각 추출물과 pH의 변화에 따른 아질산염 소거작용에 대한 결과는 pH가 낮을수록 농도가 증가할수록 소거효과는 높게 나타났고, 물 추출물의 pH 1.2에서 77.35%로 아질산염 소거능이 가장 높은 효과를 나타내었다. 에탄올, 메탄올 추출물도 물 추출물에서와 같이 pH가 낮을수록 추출물의 첨가농도가 증가할수록 아질산염 소거작용이 높았다.

• 한국식품저장유통학회지
• /
• 제18권5호
• /
• pp.739-745
• /
• 2011

실험에서는 구절초를 꽃, 잎 줄기, 뿌리 등의 부위별로 구분하여 메탄올로 추출하고 추출 수율, 총 폴리페놀 및 총 플라보노이드 함량, DPPH radical 소거능, ABTS radical 소거능, superoxide anion radical 소거능, 아질산염소거능, 철 이온에 대한 킬레이트 효과, 환원력 등을 측정하였다. 추출 수율은 꽃이 가장 높았고 총 폴리페놀 함량은 꽃에서 가장 높게 나타났고 뿌리에서 가장 낮게 나타났다. DPPH radical 소거능과 ABTS radical 소거능은 추출물의 농도가 증가함에 따라 증가하였고 꽃과 잎 줄기가 뿌리보다 높은 소거능을 나타내었다. Superoxide anion radical 소거활성도 추출물의 농도 증가에 따라 증가하여 꽃 부위가 가장 높게 나타났으나 DPPH나 ABTS radical 소거활성보다는 다소 낮은 수치를 보였다. 철 이온에 대한 킬레이트 효과는 추출물의 농도가 증가함에 따라 증가하였고 꽃에서 가장 높게 나타났고 뿌리에서 가장 낮게 나타났다. 환원력은 꽃과 잎 줄기에서 높게 나타났고 뿌리에서 낮게 나타났다. 이상의 결과를 종합하여 보면 총 폴리페놀 함량이 가장 높은 구절초 꽃이 높은 항산화활성을 보였고 그 다음으로 잎 줄기가 활성을 보임으로써 향후 천연 항산화제로서의 개발 가능성이 시시되었다.

• 생약학회지
• /
• 제44권3호
• /
• pp.224-229
• /
• 2013

Bromotopsentin (BSM) is a bisindole alkaloid compound, which is recognized as a metabolite of the marine sponge Spongosorites sp. In this study, the antioxidant activity of BSM was investigated. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, the trolox equivalent antioxidant capacity (TEAC) assay, the superoxide radical scavenging (NBT) assay, the lipid peroxidation and hydroxyl radical-induced DNA damage assays were carried out to evaluate the antioxidant activity of BSM. It was found that BSM had stronger scavenging activity on the stable free radical DPPH and superoxide radical than L-ascorbic acid with an $IC_{50}$ value of 62 and 64 ${\mu}M$, respectively. The TEAC value which indicated the total antioxidant capacity of BSM was about 0.8, which was also stronger than L-ascorbic acid. About 1.3 ${\mu}M$ of BSM induced 50% inhibition of lipid peroxidation. 60 nM of BSM exhibited a significant protective activity against DNA strand scission by hydroxyl radical on pBR322 DNA. Taken together, we suggest that BSM possesses strong antioxidant activity, and could be a valuable new addition to the list of anti-aging chemotherapeutic agents.

• Biomolecules & Therapeutics
• /
• 제8권1호
• /
• pp.32-37
• /
• 2000

Recently new soybean saponins with D DMP (2,5-dihydroxy-6-methyl-2,3,- dihydro-4H-pyran-4-one) moiety have been isolated from legumes. The purpose of this study is to characterize ROS scavenging activities of DDMP saponins ($\alpha$g, $\beta$g saponin) isolated from Glycine max (L.) Merrill. The scavenging activity on OH was examined in terms of lipid peroxidation in the rat liver homogenates and the same activity on $O_2$ was also determined in the xanthine-xanthine oxidase system, respectively. Up to 0.25 mg DDMP saponins ($\alpha$g and $\beta$g saponins) did not cause any significant effects on the prevention of lipid peroxidation as compared with the control group. In terms of superoxide scavenging activities, 0.25 and 0.5 mg $\alpha$g saponin inhibits only 2.6% and 5.5% (p<0.05) of the control group, respectively. However, $\alpha$g saponin dose-dependently (p<0.01, r=0.955) inhibits the formation of superoxide radical unto 21.3% of the control group with a maximal dose of 0.5 mg (p<0.01), equivalent to 0.17 units of superoxide dismutase activity.

• Journal of Microbiology and Biotechnology
• /
• 제25권6호
• /
• pp.795-802
• /
• 2015

This study investigates the bioactivity of tannin from amaranth (Amaranthus caudatus L.) extracts. The antioxidant activities of the extracts from amaranth leaves, flowers, and seeds were evaluated. Tannin from leaves of amaranth has been evaluated for superoxide scavenging activity by using DPPH and ABTS⁺ analysis, reducing power, protective effect against H₂O₂-induced oxidative damage in L-132 and BNL-CL2 cells, and inhibition of superoxide radical effects on HL-60 cells. At a concentration of 100 µg/ml, tannin showed protective effects and restored cell survival to 69.2% and 41.8% for L-132 and BNL-CL2 cells, respectively. Furthermore, at the same concentration, tannin inhibited 41% of the activity of the superoxide radical on HL-60 cells and 43.4% of the increase in nitric oxide levels in RAW 264.7 cells. The expression levels of the antioxidant-associated protein SOD-1 were significantly increased in a concentration-dependent manner in RAW 264.7 cells treated with tannin from amaranth leaves. These results suggest that tannin from the leaves of Amaranthus caudatus L. is a promising source of antioxidant component that can be used as a food preservative or nutraceutical.

• 대한화장품학회지
• /
• 제35권3호
• /
• pp.251-256
• /
• 2009

개가시나무 가지의 에탄올 추출물에서 항산화활성을 관찰하였으며, 활성성분을 규명하기 위한 연구를 진행하였다. 그 결과, 4종류의 화합물을 분리하여 동정하였다. 분리 동정된 성분은 catechin(1), epi-catechin(2), tyrosol(3) 및 tiliroside(4)이다. 분리 성분의 항산화활성은 DPPH 라디칼 및 superoxide 음이온 라디칼 소거활성을 이용하여 측정하였다. 화합물 1, 2, 3, 4는 $100{\mu}L$/mL 농도에서 각각 94.2 %, 93.4 %, 33.6 %, 11.2 %의 DPPH 라디칼 저해활성을 나타내었다. 또한, 화합물 1, 2, 3, 4는 $200{\mu}L$/mL 농도에서 각각 60.2 %, 35.1 %, 20.6%, 4.5 %의 superoxide 음이온 라디칼 저해활성을 나타내었다. 화합물 $1{\sim}4$는 개가시나무에서는 처음으로 분리된 물질이다.

• 생약학회지
• /
• 제43권4호
• /
• pp.297-301
• /
• 2012

In the course of screening for antioxidant compounds by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH), a total extract of the twigs of Taxillus delavayi (Loranthaceae) was found to show potent radical scavenging activity. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of two phenolic compounds, catechin (1) and galloyl catechin (2), as active principles. Their structures were elucidated by spectroscopic studies. These two compounds showed the significant antioxidative effects on DPPH, and riboflavin- and xanthin-originated superoxide quenching activities. And these two compounds also showed strong nitric oxide radical scavenging effects. In riboflavin- and xanthin-originated superoxide quenching activities and nitric oxide radical scavenging effect, compound 2 showed better antioxidant activities than vitamin C.