• 한국지반공학회논문집
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• 제25권10호
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• pp.111-122
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• 2009

지반에 포함된 입자의 용해작용은 흙의 강도를 저하시키고, 지하구조물의 침하를 발생시킨다. 본 논문에서는 입자용해시 혼합재의 물리적 특성 변화를 조사하고자 하였다. 침하량 측정과 전단파 측정과 같은 거시적 반응을 평가하기 위해, 소금과 모래로 구성된 혼합재를 사용하였다. 또한, 광탄성(photoelastic) 디스크와 얼음 디스크 혼합재를 이용하여 힘 연결고리(force chain), 간극, 접촉점수(coordination number), 그리고 수평력 변화와 같은 미시적 거동을 분석하였다. 소금-모래 혼합재에서 전단파는 압밀셀에 설치된 다단의 벤더 엘리먼트로 측정되었고, 광탄성 디스크-얼음 디스크 혼합재의 역학적 거동은 로드셀과 디지털 이미지를 이용하여 분석되었다. 실험결과, 소금의 용해시 전단파 속도는 초기에는 감소한 후에 증가한 후 일정한 값으로 수렴하였다. 용해가능 입자의 크기가 커질수록 그리고 입자패킹이 불규칙해질수록 입자용해시 수평력의 변화가 더 커지는 것을 알 수 있었다. 용해 후, 간극비는 증가했고, 접촉점수는 감소하였다. 본 연구에서는 힘 연결고리, 간극비, 접촉점수와 같은 입자의 거동변화가 전단파 속도와 전체시스템의 수평력 변화 같은 전체적인 거동에 영향을 주는 것으로 나타났다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권2호
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• pp.143-146
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• 2004

A surface plasmon resonance (SPR) imaging system was constructed and used to detect the hexahistidine-ubiquitin-tagged human parathyroid hormone fragment (His$\sub$6/-Ub-hPTHF(1-34)) expressed in Escherichia coli. The hexahistidine-specific antibody was immobilized on a thin gold film coated with ProLinker$\^$TM/ B, a novel calixcrown derivative with a bifunctional coupling property that permits efficient immobilizaton of capture proteins on solid matrices. The soluble and insoluble fractions of an E. coli cell lysate were spotted onto the antibody-coated gold chip, which was then washed with buffer (pH 7.4) solution and dried. SPR imaging measurements were carried out to detect the expressed His$\sub$6/-Ub-hPTHF(1-34). There was no discernible protein image in the uninduced cell lysate, indicating that non-specific binding of contaminant proteins did not occur on the gold chip surface. It is expected that the approach used here to detect affinity-tagged recombinant proteins using an SPR imaging technique could be used as a powerful tool for the analyses of a number of proteins in a high-throughput mode.

• 동아시아식생활학회지
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• 제22권5호
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• pp.649-656
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• 2012

We investigated the quality characteristics of dohwaju (fermented with peach flowers and branches, a Korean traditional alcoholic beverage) containing different sub-ingredients. During fermentation, the inner temperatures of the added sub-ingredient treatments were higher compared to non-treatment, and only peach branches treatment. showed the highest inner temperature. There were no significant differences in the physicochemical results among the treatments. pH decreased from the first mashing day to the second and then increased slightly until fermentation was terminated. Total acidities showed opposite patterns as compared to pH level. Amino acid contents, soluble solids, and alcohol contents increased steadily until the end of fermentation, whereas reducing sugar contents showed the opposite trend. In a preference survey, DB (only peach branches)-treated dowhaju showed the highest evaluation in terms of taste and overall acceptability with walues of $5.07{\pm}1.38$ and $5.57{\pm}1.16$, respectively. Based on the results of this study, we concluded that only peach branches treatment is optimal for the manufacture of dowhaju. We expect that the quality of dowhaju can be improved by providing basic information concerning its manufacture.

• BMB Reports
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• 제40권4호
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• pp.562-570
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• 2007

Soluble or cell-bound IL-1 receptor accessory protein (IL-1RAcP) does not bind IL-1 but rather forms a complex with IL-1 and IL-1 receptor type I (IL-1RI) resulting in signal transduction. Synthetic peptides to various regions in the Ig-like domains of IL-1RAcP were used to produce antibodies and these antibodies were affinity-purified using the respective antigens. An anti-peptide-4 antibody which targets domain III inhibited 70% of IL-$1\beta$-induced productions of IL-6 and PGE2 from 3T3-L1 cells. Anti-peptide-2 or 3 also inhibited IL-1-induced IL-6 production by 30%. However, antipeptide-1 which is directed against domain I had no effect. The antibody was more effective against IL-$1\beta$ compared to IL-$1\alpha$. IL-1-induced IL-6 production was augmented by coincubation with PGE2. The COX inhibitor ibuprofen blocked IL-1-induced IL-6 and PGE2 production. These results confirm that IL-1RAcP is essential for IL-1 signaling and that increased production of IL-6 by IL-1 needs the co-induction of PGE2. However, the effect of PGE2 is independent of expressions of IL-1RI and IL-1RAcP. Our data suggest that domain III of IL-1RAcP may be involved in the formation or stabilization of the IL-1RI/IL-1 complex by binding to epitopes on domain III of the IL-1RI created following IL-1 binding to the IL-1RI.

• 생약학회지
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• 제46권3호
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• pp.214-222
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• 2015

Recently, many studies have focused on the aging and oxidative stress. Several papers reported that Vigna angularis has various biological properties including antiaging, antioxidative and anti-inflammatory activities. Methanol extract from the red bean sprouts was successively partitioned as n-hexane, methylene chloride, ethyl acetate, n-butanol and H₂O soluble fractions. We had studied lifespan extending and stress resistant effects of the fractions using Caenorhabditis elegans. Superoxide dismutase (SOD), catalase activities, and intracellular reactive oxygen species (ROS) levels were also investigated. Moreover, we had studied to find any significant change in aging-related factors such as reproduction, food intake, growth and movement of C. elegans. Our results represent that ethyl acetate fraction showed the most potent lifespan extending and stress resistant effects, and this fraction was able to elevate SOD and catalase activities of worms, and reduce intracellular ROS accumulation.

• Asian Journal of Atmospheric Environment
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• 제11권2호
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• pp.79-95
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• 2017

A number of field studies have provided evidence that biomass burning is one of the major global sources of atmospheric particles. In this study, we have collected $PM_{2.5}$ emitted from biomass burning combusted at open burning and laboratory chamber situations. The open burning experiment was conducted with the cooperation of 9 farmers in Chiba Prefecture, Japan, while the chamber experiment was designed to evaluate the characteristics of chemical components among 14 different plant species. The analyzed categories were $PM_{2.5}$ mass concentration, organic carbon (OC), elemental carbon (EC), ionic components ($Na^+$, ${NH_4}^+$, $Ca^{2+}$, $Mg^{2+}$, $K^+$, $Cl^-$, ${NO_3}^-$ and ${SO_4}^{2-}$), water-soluble organic carbon (WSOC), water-insoluble inorganic carbon (WIOC), char-EC and soot-EC. OC was the dominant chemical component, accounting for the major fraction of primary $PM_{2.5}$ derived from biomass burning, followed by EC. Ionic components contributed a small portion of $PM_{2.5}$, as well as that of $K^+$. In some cases, $K^+$ is used as biomass burning tracer; however, the observations obtained in this study suggest that $K^+$ may not always be suitable as a tracer for biomass burning emissions. Also, the results of all the samples tested indicate relatively low values of char-EC compared to soot-EC. From our results, careful consideration should be given to the usage of $K^+$ and char-EC as indicators of biomass burning. The calculated ratios of WSOC/OC and WIOC/OC were 55.7% and 44.3% on average for all samples, which showed no large difference between them. The organic materials to OC ratio, which is often used for chemical mass closure model, was roughly estimated by two independent methods, resulting in a factor of 1.7 for biomass burning emissions.

• 한국환경농학회지
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• 제35권2호
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• pp.128-136
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• 2016

BACKGROUND: Hydrothermal carbonization reaction is the thermo-chemical energy conversion technology for producing the solid fuel of high carbon density from organic wastes. The hydrothermal carbonization reaction is accompanied by the thermal hydrolysis reaction which converse particulate organic matters to soluble forms (hydro-thermal hydrolysate). Recently, hydrothermal carbonization is adopted as a pre-treatment technology to improve anaerobic digestion efficiency. This research was carried out to assess the effects of hydro-thermal reaction temperature on the methane potential and anaerobic biodegradability in the thermal hydrolysate of organic sludge generating from the wastewater treatment plant of poultry slaughterhouse .METHODS AND RESULTS: Wastewater treatment sludge cake of poultry slaughterhouse was treated in the different hydro-thermal reaction temperature of 170, 180, 190, 200, and 220℃. Theoretical and experimental methane potential for each hydro-thermal hydrolysate were measured. Then, the organic substance fractions of hydro-thermal hydrolysate were characterized by the optimization of the parallel first order kinetics model. The increase of hydro-thermal reaction temperature from 170℃ to 220℃ caused the enhancement of hydrolysis efficiency. And the methane potential showed the maximum value of 0.381 Nm³ kg^-1-VS_added in the hydro-thermal reaction temperature of 190℃. Biodegradable volatile solid(VSB) content have accounted for 66.41% in 170℃, 72.70% in 180℃, 79.78% in 190℃, 67.05% in 200℃, and 70.31% in 220℃, respectively. The persistent VS content increased with hydro-thermal reaction temperature, which occupied 0.18% for 170℃, 2.96% for 180℃, 6.32% for 190℃, 17.52% for 200℃, and 20.55% for 220℃.CONCLUSION: Biodegradable volatile solid showed the highest amount in the hydro-thermal reaction temperature of 190℃, and then, the optimum hydro-thermal reaction temperature for organic sludge was assessed as 190℃ in the aspect of the methane production. The rise of hydro-thermal reaction temperature caused increase of persistent organic matter content.

• 한국농공학회지
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• 제20권2호
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• pp.4695-4707
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• 1978

The objeative of this study is to study how to rapidly convert tidal land into cultivable land. The study of a rapid, reasonable desalinization method is conducted at Namyang tidal land which represents soil texture of tidal lands along the south west costa larea of Korea. Therefore, Researches were made at many Pilots in order to find a way of high efficiency of leaching with simpler facilities and cheaper costs. The results of study are briefly summarized as follaws: 1. Subdrainage efficieny is 35%. This is a Poorly drained area, and needs longer leaching desalinization period. 2. The efficieny of desalinization in P.V.C 16 meters plot is the same as that of mole drainage 2 meters plot. P.V.C 4 meters plot has desalinization effect as much as two times compared to P.V.C 16 meters plot. 3. Because the soil texture is silty-clay, desalinization in non-treated plot of sub-drainage and surface drainage desalinization take three times longer period in comparision with P.V.C 4 meters plot. 4. As to the desalinization rate of soluble salt in the soil, the efficieny of desalinization of the topsoil in P.V.C plots was 50% higher than that of mole drainage plot and about 170% higher than that of non-treated plot. In the deep soil salt accumulation at topsoil was observed in non-treated and mole drainage plots, but efficiency in P.V.C polt is about 40 times as high as that of mole drainage and non-treated plot. 5. As to the results of use gypsum and lime as sub-drainage soil improver, gypsum was 60% more efficieny than lime in the continuously inundated plot and 44% in the intermittently inundated plot. The efficieny of gypsum and lime in the intermittently flooded plot is 35% and 42% higher than that of continuously flooded plot reapeaticee1y.

• 한국대기환경학회지
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• 제31권4호
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• pp.345-355
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• 2015

Carbonaceous compounds in the atmospheric particulate matter with an aerodynamic diameter of less than or equal to a nominal $10{\mu}m$ ($PM_{10}$) were analyzed for the samples collected during the period of August 2006 to August 2007 at Jongro in Seoul. A total 18 dicarboxylic acids (DCAs) and levoglucosan, as well as organic carbon (OC), elemental carbon (EC), and water soluble organic carbon (WSOC), were analyzed. Distinctive seasonal patterns of the concentrations of OC, EC, and WSOC including levoglucosan were observed with the highest concentrations in winter and the lowest concentrations in summer. In addition, OC, WSOC, and most of DCAs showed also higher concentration in summer than in winter. Using the seasonal patterns and relevant indicative ratios (WSOC/EC and $OC_{sec}/OC_{tot}$) of the carbonaceous compounds, it was verified that (1) primary emission sources were elevated in winter, and (2) the formation of secondary OC increased due to the prompted photochemical reaction in summer. Results from this study also suggest that some organic compounds were likely attributed to longrange transport.

• Asian-Australasian Journal of Animal Sciences
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• 제28권5호
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• pp.691-696
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• 2015

The objective of this study was to evaluate the quality of five commercial enzyme linked immunosorbent assay (ELISA) kits (A, B, C, D, and E) from different suppliers for detecting aflatoxin $B_1$ ($AFB_1$). $AFB_1$-free corn samples supplemented with different levels of $AFB_1$ (5, 10, and $20{\mu}g/kg$) were used as positive controls and 6 replicates of each control sample were tested to evaluate the accuracy and precision of these kits. In addition, we also evaluated the performance of these ELISA kits for $AFB_1$ in 30 feed samples, including corn, distillers dried grains with soluble, wheat samples, soybean meal, and poultry feed, which were verified by high performance liquid chromatography. Results showed that the coefficients of variation ranged from 1.18% to 16.22% in intra-plate and 2.85% to 18.04% in inter-plate for the determination of $AFB_1$. The half maximal inhibitory concentration for five kits ranged from 3.72 to $7.22{\mu}g/kg$. The quantitation limits of $AFB_1$ were all under the legal limit in China but somewhat inconsistent with kit instructions. Although the recovery rate of four of the five kits were either less than 90% or more than 110%, all these values were acceptable in practice. Two kits had high false positive rates (C and E). In conclusion, our results revealed that the qualities of five tested ELISA kits were significantly different.