• Microbiology and Biotechnology Letters
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• v.36 no.2
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• pp.149-157
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• 2008

Many proteins consist of distinctive domains that can act independently or cooperatively to achieve a unique function. As these domains evolve from a naturally existing repertoire of functional domains, this implies that domain organization is an intrinsic element involved in building the complex structure and function of proteins. Thus, identifying functional domains would appear to be critical to the elucidation of questions related to protein evolution, folding, and the engineering of hybrid proteins for tai- lored applications. However, the simple application of "Lego-like assembly" to the engineering of hybrid proteins is an oversimplification, as many hybrid constructs lack structural stability, usually due to unfavorable domain contacts. Thus, directed evolution, along with computational studies, may help to engineer hybrid proteins with improved physico-chemical properties. Accordingly, this paper introduces several approaches to functional hybrid protein engineering that potentially can be used to create modulators of gene transcription and cell signaling, and novel biosensors to analyze biological functions in vivo.

• Journal of Adhesion and Interface
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• v.7 no.4
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• pp.60-64
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• 2006

In modern vehicle construction the search for means of weight reduction, improving durability, increasing comfort and raising body stiffness are issues of priority to the design engineer. The intelligent usage of many materials such as high strength steel, light-alloys and plastics enables a significant vehicle weight reduction to be achieved. The classical joining techniques used in the automobile industry need to be newly-evaluated since they often do not present workable solutions for such mixed-material connections, for example aluminium/steel. Calculation/simulation methods have made progress as a key factor for broader and more cost-effective implementation of structural bonding. This will lead to reduction of spotwelds and accelerate the car development. A special focus of the paper is the use of high strength steel grades. It will be shown that adhesive bonding is a key tool for yielding the potential of advanced high strength steel for low gauging without compromising the stiffness. The latest status of adhesive development has been described. Improvements with physical strength and glass temperature as well as of process relevant properties are shown. Also the situation regarding occupational hygiene is treated, showing that by further spotweld point reduction the emission around the working area can be even lowered against the current praxis. High performing lightweight design cannot longer do without high performing crash durable adhesives.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.16 no.4
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• pp.9-15
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• 2017

In hot forming process, the dies in which excessive worn or crack occurs is reused after repair. Generally hot forming dies are recycled through a welding repair method. Welding repair methods are highly dependent on the skills of engineer. It causes process defects such as dimensional defects and structural defects. Recently, the metal 3D printing method has been applied to the repair of used dies. The aim of this study is to evaluate the wear characteristics of AISI H13 tool steel repaired by 3D printing method. Three kinds of wear specimens were fabricated by using 3D printing, welding, and initial material. A pin-on-disk wear test was carried out to evaluate the wear characteristics. From the result of wear test, the wear characteristics of 3D printing method was superior to that of the welded material, and was similar to that of the initial material.

• Food Science and Biotechnology
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• v.18 no.2
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• pp.396-401
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• 2009

The nucleotide sequence contains 2 open reading frames encoding a 45-amino-acid protein homologous to a transcriptional repressor protein CopG, and a 203-amino-acid protein homologous to a replication protein RepB. Putative countertranscribed RNA, a double-strand origin, and a single-strand origin were also identified. A shuttle vector, pUCL2.1, for various lactic acid bacteria (LAB) was constructed on the basis of the pCL2.1 replicon, into which an erythromycin-resistance gene as a marker and Escherichia coli ColE1 replication origin were inserted. pUCL2.1 was introduced into E. coli, Lc. lactis, Lactobacillus (Lb.) plantarum, Lb. paraplantarum, and Leuconostoc mesenteroides. The recombinant LAB maintained traits of transformed plasmid in the absence of selection pressure over 40 generations. Therefore, pUCL2.1 could be used as an E. coli/LAB shuttle vector, which is an essential to engineer recombinant LAB strains that are useful for food fermentations.

• Smart Structures and Systems
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• v.7 no.3
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• pp.213-222
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• 2011

The native extracellular matrix (ECM) consists of an integrated fibrous protein network and proteoglycan-based ground (hydrogel) substance. We designed a novel electrospinning technique to engineer a three dimensional fiber-hydrogel composite that mimics the native ECM structure, is injectable, and has practical macroscale dimensions for clinically relevant tissue defects. In a model system of articular cartilage tissue engineering, the fiber-hydrogel composites enhanced the biological response of adult stem cells, with dynamic mechanical stimulation resulting in near native levels of extracellular matrix. This technology platform was expanded through structural and biochemical modification of the fibers including hydrophilic fibers containing chondroitin sulfate, a significant component of endogenous tissues, and hydrophobic fibers containing ECM microparticles.

• Journal of Korean Society of Steel Construction
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• v.11 no.2 s.39
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• pp.131-142
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• 1999

The general behavior of the curved girder including the warping effects can be presented as the series of differential equations developed by Vlasov. Generally, bending moment is the most important factor for engineer to decide the section of the girder. In order to accommodate easiness of the structural analysis for the curved girder bridge, this paper suggest the ratios of bending moment of curved gilder to that of straight girder. These ratios are presented by an approximate formula setting central angle ${\theta}(L/R)$ as a variable. The approximate formula of the maximum bending moment ratios and influence lines of all stress resultants can be used to design the three-span curved girder bridges.

• Earthquakes and Structures
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• v.7 no.1
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• pp.39-55
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• 2014

A modified procedure is presented for assessing the seismic response of elastic non-proportionate multistory buildings. This procedure retains the simplicity of the methodology presented by the author in earlier papers, but it presents higher accuracy in buildings composed by very dissimilar types of bents. As a result, not only frequencies and peak values of base resultant forces are determined with higher accuracy, but also the location of the first mode center of rigidity (m1-CR). The closeness of m1-CR with the axis passing through the centers of floor masses (mass axis) implies a reduced rotational response and it is demonstrated that in elastic systemsa practically translational response is obtained when this point lies on the mass axis.Besides, when common types of buildings are detailed as planar structures under a code load, this response is maintained in the inelastic phase of their response as a result of the almost concurrent yielding of all the resisting bents. This property of m1-CR can be used by the practicing engineer as a guideline to form a structural configuration which will sustain minimum rotational response, simply by allocating the resisting elements in such a way that this point lies close to the mass axis. Inelastic multistory building structures, detailed as above, may be regarded as torsionally balanced multistory systems and this is demonstrated in eight story buildings, composed by dissimilar bents, under the ground motions of Kobe 1995 (component KJM000) and Friuli 1976 (component Tolmezzo E-W).

• Journal of the Korean Society of Propulsion Engineers
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• v.5 no.3
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• pp.79-86
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• 2001

The six-components force measurements systems for rocket motors are used to measure multi components force generated by TVC(Thrust Vector Control) motors. This paper suggested the modeling scheme which is used in preliminary design and test analysis procedure and which can be applied to the existing other test stand in operation. The model whose parameters are determined by least square method makes the design engineer build the test stand to satisfy all kinds of requirements such as accuracy, operating condition and structural stability without tradeoff among the requirements. The experimental results shows that the proposed model has better accurate performances than those of other existing model.

• Structural Engineering and Mechanics
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• v.54 no.6
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• pp.1097-1109
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• 2015

A novel optimization approach for reinforced concrete (RC) biaxially loaded columns is proposed. Since there are several design constraints and influences, a new computation methodology using iterative analyses for several stages is proposed. In the proposed methodology random iterations are combined with music inspired metaheuristic algorithm called harmony search by modifying the classical rules of the employed algorithm for the problem. Differently from previous approaches, a detailed and practical optimum reinforcement design is done in addition to optimization of dimensions. The main objective of the optimization is the total material cost and the optimization is important for RC members since steel and concrete are very different materials in cost and properties. The methodology was applied for 12 cases of flexural moment combinations. Also, the optimum results are found by using 3 different axial forces for all cases. According to the results, the proposed method is effective to find a detailed optimum result with different number of bars and various sizes which can be only found by 2000 trial of an engineer. Thus, the cost economy is provided by using optimum bars with different sizes.

• Molecules and Cells
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• v.38 no.12
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• pp.1105-1110
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• 2015

Phleichrome, a pigment produced by the phytopathogenic fungus Cladosporium phlei, is a fungal perylenequinone whose photodynamic activity has been studied intensively. To determine the biological function of phleichrome and to engineer a strain with enhanced production of phleichrome, we identified the gene responsible for the synthesis of phleichrome. Structural comparison of phleichrome with other fungal perylenequinones suggested that phleichrome is synthesized via polyketide pathway. We recently identified four different polyketide synthase (PKS) genes encompassing three major clades of fungal PKSs that differ with respect to reducing conditions for the polyketide product. Based on in silico analysis of cloned genes, we hypothesized that the non-reducing PKS gene, Cppks1, is involved in phleichrome biosynthesis. Increased accumulation of Cppks1 transcript was observed in response to supplementation with the application of synthetic inducer cyclo-(${_L}-Pro-{_L}-Phe$). In addition, heterologous expression of the Cppks1 gene in Cryphonectria parasitica resulted in the production of phleichrome. These results provide convincing evidence that the Cppks1 gene is responsible for the biosynthesis of phleichrome.