Browse > Article

Structural Analysis of Plasmid pCL2.1 from Lactococcus lactis ssp. lactis $ML_8$ and the Construction of a New Shuttle Vector for Lactic Acid Bacteria  

Jeong, Do-Won (Research Institute for Agriculture and Life Sciences, Seoul National University)
Cho, San-Ho (Department of Agricultural Biotechnology, Seoul National University)
Lee, Jong-Hoon (Department of Food Science and Biotechnology, Kyonggi University)
Lee, Hyong-Joo (Department of Agricultural Biotechnology, Seoul National University)
Publication Information
Food Science and Biotechnology / v.18, no.2, 2009 , pp. 396-401 More about this Journal
Abstract
The nucleotide sequence contains 2 open reading frames encoding a 45-amino-acid protein homologous to a transcriptional repressor protein CopG, and a 203-amino-acid protein homologous to a replication protein RepB. Putative countertranscribed RNA, a double-strand origin, and a single-strand origin were also identified. A shuttle vector, pUCL2.1, for various lactic acid bacteria (LAB) was constructed on the basis of the pCL2.1 replicon, into which an erythromycin-resistance gene as a marker and Escherichia coli ColE1 replication origin were inserted. pUCL2.1 was introduced into E. coli, Lc. lactis, Lactobacillus (Lb.) plantarum, Lb. paraplantarum, and Leuconostoc mesenteroides. The recombinant LAB maintained traits of transformed plasmid in the absence of selection pressure over 40 generations. Therefore, pUCL2.1 could be used as an E. coli/LAB shuttle vector, which is an essential to engineer recombinant LAB strains that are useful for food fermentations.
Keywords
pCL2.1; shuttle vector; lactic acid bacteria;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Shanahan F. Probiotics in inflammatory bowel disease--therapeutic rationale and role. Adv. Drug Deliver Rev. 56: 809-818 (2004)   DOI   PUBMED   ScienceOn
2 Kim JY, Lee S, Jeong DW, Hachimura S, Kaminogawa S, Lee HJ. Effects of intraperitoneal administration of Lactococcus lactis ssp. lactis cellular fraction on immune response. Food Sci. Biotechnol. 14: 405-409 (2005)
3 Kim JY, Lee S, Jeong DW, Hachimura S, Kaminogawa S, Lee HJ. In vivo immunopotentiating effects of cellular components from Lactococcus lactis ssp. lactis. J. Microbiol. Biotechn. 16: 786-790 (2006)
4 Pouwels PH, Leer RJ. Genetics of lactobacilli: Plasmids and gene expression. Anton. Leeuw. Int. J. G. 64: 85-107 (1993)
5 Pouwels PH, Vriesema A, Martinez B, Tielen FJ, Seegers JF, Leer RJ, Jore J, Smit E. Lactobacilli as vehicles for targeting antigens to mucosal tissues by surface exposition of foreign antigens. Method Enzymol. 336: 369-389 (2001)   DOI
6 De Vos WM, Simons G. Gene cloning and expression systems in Lactococci. pp. 52-105. In: Genetics and Biotechnology of Lactic Acid Bacteria. Gasson MJ, De Vos WM (eds). Chapman and Hall, Glasgow, New Zealand (1994)
7 Chang HC, Choi YD, Lee HJ. Nucleotide sequence of a plasmid pCL2.1 from Lactococcus lactis ssp. lactis ML8. Plasmid 34: 234-235 (1995)   DOI   ScienceOn
8 Coffey A, Harrington A, Kearney K, Daly C, Fitzgerald G. Nucleotide sequence and structural organization of the small, broadhost- range plasmid pCI411 from Leuconostoc lactis 533. Microbiology 140: 2263-2269 (1994)   DOI   PUBMED
9 Kim TW, Lee JY, Jung SH, Kim YM, Jo JS, Chung DK, Lee HJ, Kim HY. Identification of lactic acid bacteria in kimchi using SDSPAGE profiles of whole cell proteins. J. Microbiol. Biotechn. 12:635-642 (2002)
10 Acebo P, Garcia de Lacoba M, Rivas G, Andreu JM, Espinosa M, del Solar G. Structural features of the plasmid pMV158-encoded transcriptional repressor CopG, a protein sharing similarities with both helix-turn-helix and beta-sheet DNA binding proteins. Proteins 32: 248-261 (1998)   DOI   ScienceOn
11 Kodaira K, Oki M, Taketo A, Yasukawa H, Masamune Y. Determination of the single strand origin of Shigella sonnei plasmid pKYM. Biochim. Biophys. Acta 1260: 183-190 (1995)   DOI   PUBMED
12 Novick RP. Staphylococcal plasmids and their replication. Annu. Rev. Microbiol. 43: 537-565 (1989)   DOI   PUBMED   ScienceOn
13 De Vos WM, Hugenholtz J. Engineering metabolic highways in Lactococci and other lactic acid bacteria. Trends Biotechnol. 22: 72-79 (2004)   DOI   ScienceOn
14 Boe L, Gros MF, te Riele H, Ehrlich SD, Gruss A. Replication origins of single-stranded-DNA plasmid pUB110. J. Bacteriol. 171:3366-3372 (1989)   DOI
15 Tinoco I, Borer PN, Dengler B, Levin MD, Uhlenbeck OC, Crothers DM, Bralla J. Improved estimation of secondary structure in ribonucleic acids. Nature-New Biol. 246: 40-41 (1973)   DOI   PUBMED
16 Khan SA. Plasmid rolling-circle replication: Recent developments. Mol. Microbiol. 37: 477-484 (2000)   PUBMED
17 Arnau J, Hjerl-Hansen E, Israelsen H. Heterologous gene expression of bovine plasmin in Lactococcus lactis. Appl. Microbiol. Biotechnol. 48: 331-338 (1997)   DOI   ScienceOn
18 Seegers JF, Zhao AC, Meijer WJ, Khan SA, Venema G, Bron S. Structural and functional analysis of the single-strand origin of replication from the lactococcal plasmid pWV01. Mol. Gen. Genet. 249: 43-50 (1995)   DOI   ScienceOn
19 Ribeiro LA, Azevedo V, Le Loir Y, Oliveira SC, Dieye Y, Piard JC, Gruss A, Langella P. Production and targeting of the Brucella abortus antigen L7/L12 in Lactococcus lactis: A first step towards food-grade live vaccines against brucellosis. Appl. Environ. Microb. 68: 910-916 (2002)   DOI   ScienceOn
20 Jeong DW, Hwang ES, Lee HJ. Food-grade expression and secretion system in Lactococcus. Food Sci. Biotechnol.15: 485-493 (2006)
21 Del Solar GH, Puyet A, Espinosa M. Initiation signals for the conversion of single stranded to double stranded DNA forms in the streptococcal plasmid pLS1. Nucleic Acids Res. 15: 5561-5580 (1987)   DOI   ScienceOn
22 Bengmark S, Martindale R. Prebiotics and synbiotics in clinical medicine. Nutr. Clin. Pract. 20: 244-261 (2005)   DOI   ScienceOn
23 Nouaille S, Ribeiro LA, Miyoshi A, Pontes D, Le Loir Y, Oliveira SC, Langella P, Azevedo V. Heterologous protein production and delivery systems for Lactococcus lactis. Genet. Mol. Res. 2: 102-111 (2003)
24 Gruss AD, Ross HF, Novick RP. Functional analysis of a palindromic sequence required for normal replication of several staphylococcal plasmids. P. Natl. Acad. Sci. USA 84: 2165-2169 (1987)   DOI   ScienceOn
25 Simon D, Chopin A. Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis. Biochimie 70:559-566 (1988)   DOI   ScienceOn
26 Holo H, Nes IF. High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl. Environ. Microb. 55: 3119-3123 (1989)   PUBMED
27 Gasson MJ. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J. Bacteriol. 154: 1-9 (1983)
28 Khan SA. Plasmid rolling-circle replication: Highlights of two decades of research. Plasmid 53: 126-136 (2005)   DOI   PUBMED   ScienceOn
29 Roberts RC, Burioni R, Helinski DR. Genetic characterization of the stabilizing functions of a region of broad-host range plasmid RK2. J. Bacteriol. 172: 6204-6216 (1990)   DOI
30 Kim SY, Lee KW, Kim JY, Lee HJ. Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU-1 stomach adenocarcinoma cells. Biofactors 22: 119-122 (2004)   DOI   PUBMED
31 Dodd IB, Egan JB. Improved detection of helix-turn-helix DNAbinding motifs in protein sequences. Nucleic Acids Res. 18: 5019-5026 (1990)   DOI   ScienceOn
32 Madsen SM, Andrup L, Boe L. Fine mapping and DNA sequence of replication functions of Bacillus thuringiensis plasmid pTX14-3. Plasmid 30: 119-130 (1993)   DOI   ScienceOn
33 Lee JH, Kim MJ, Jeong DW, Kim MJ, Kim JH, Chang HC, Chung DK, Kim HY, Kim KH, Lee HJ. Identification of bacteriocinproducing Lactobacillus paraplantarum-First isolated from kimchi. J. Microbiol. Biotechn. 15: 428-433 (2005)
34 Cotter PD, Hill C, Ross RP. Bacteriocins: Developing innate immunity for food. Nat. Rev. Microbiol. 3: 777-788 (2005)   DOI   ScienceOn
35 Hanahan D. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580 (1983)   DOI   PUBMED
36 Alonso JC, Taylor RH. Initiation of plasmid pC194 replication and its control in Bacillus subtilis. Mol. Gen. Genet. 210: 476-484 (1987)   DOI   ScienceOn