• Journal of the Korean Recycled Construction Resources Institute
• /
• v.6 no.4
• /
• pp.252-258
• /
• 2018

A larger energy consumption and concentration of population induced green house gas glowing and heat island effect in the urban space. Roof green system was a effect method to reduce green house gas and heat concentration in the city. Therefore, construction of this system was increasing. Most of lightweight soil used in roof green system was perlite, but this caused dust and skin disease. So it needed to develop another new lightweight soli for roof green system. Meanwhile, a thermoelectric power plant generated bottom ash as a by-product. According to previous research, bottom ash could be used for artificial lightweight soil with 60 wt% of mixing rate. But this study was proceed to develop a artificial lightweight soil using bottom ash with higher mixing rate by 65 wt% and different organic ingredients. First, physical and chemical properties of bottom ash was investigated. Then test according to landscaping design standard was proceeded for various artificial lightweight soil mix types using bottom ash, bark, compost and coco peat. As a result, the artificial lightweight soil with 65% of bottom ash, 30% of bark and 5% of compost was suitable for low and middle range of soil standard.

• Korean Journal of Clinical Laboratory Science
• /
• v.51 no.1
• /
• pp.26-33
• /
• 2019

Recently, the rapid growth of the companion animal market has led to the development of animal disease diagnosis kits. Therefore, the utility of the introduction of biomarkers for the development of animal molecular diagnostics is being reevaluated. A good biomarker should be precise and reliable, distinguish between normal and diseased states, and differentiate between different diseases. Recently reported genetic markers, tumor markers (cell free DNA, circulating tumor cells, granzyme, and skin tumors), and others (brucellosis, programmed death recovery-1, symmetric dimethylarginine, periostin, and cysteinyl leukotrien) have been developed. The biomarkers are used for risk prediction or for the screening, diagnosis, and monitoring of disease progression. The most important criteria for related biomarkers are disease specificity. Many potential biomarkers have emerged from laboratory and test studies, but they have not been validated in independent or large-scale clinical studies. Candidate biomarkers evaluate disease associations, verify the effectiveness of biomarkers for early detection and disease progression, and incorporate them into humans and animals. In the future, it will be necessary to reevaluate the utility of well-structured biomarker-based research and study the development of kits that can be used in on-site tests in accordance with the trends introduced in the diagnosis of animal diseases.

• Journal of The Korean Society of Emergency Medicine
• /
• v.29 no.6
• /
• pp.649-655
• /
• 2018

Objective: This paper reports the possibility of using of a locking horizontal mattress suture technique in repairing lacerations that are difficult to suture with staples. Methods: Data were collected retrospectively over a 6-month period regarding the routine repair of scalp lacerations: those in areas injured by a high energy blunt mechanism, continued to bleed after pressure, nonlinear or damaged skin repaired with a locking horizontal mattress technique, and simple interrupted technique. The effects of the two techniques used to repair scalp lacerations on wound healing, complication rate, and patient satisfaction were examined. The categorical variables are expressed as the number and percent. A Mann-Whitney-Wilcoxon test was used for statistical analysis. A P-value less than 0.05 was considered significant. Results: Thirty-seven consecutive patients with scalp lacerations presented for care. Wound closure was accomplished with the locking horizontal mattress sutures in 40.5% (n=15) (median length, 5.0 cm; interquartile range [IQR], 4.0-7.0 cm). Simple interrupted sutures (median length, 4 cm; IQR, 3.0-5.0 cm) were used in 59.5% (n=22) (P=0.015). The frequency of additional bandage compression (P=0.008), frequency of exudative hemorrhage (P=0.018), and suture mark frequency at suture removal (P=0.047) were significantly lower in the locking horizontal mattress group. Conclusion: The locking horizontal mattress suture, which has the advantage of a horizontal mattress suture, may be one of the ways that can be used alternatively to treat scalp lacerations that difficult to suture with staples.

• Journal of the Korean institute of surface engineering
• /
• v.55 no.6
• /
• pp.390-397
• /
• 2022

Parylene-C which was mainly used for industries such as electronics, machinery and semiconductors has recently been in the spotlight in the medical field due to its properties such as corrosion resistance and biocompatibility. In this study we intend to derive a plan to improve the bonding strength of Parylene-C coating with the SUS304 base material for medical use which can be applied to various medical fields such as needles, micro needles and in vitro diagnostic device sensors. Through plasma pretreatment the bonding strength between Parylene-C and metal materials was improved. It was confirmed that the coated surface was hydrophobic by measuring the contact angle and the improvement of the surface roughness of the sample manufactured through CNC machining was confirmed by measuring the surface roughness with SEM. Through the above results, it is thought that it will be effective in increasing usability and reducing pain in patients by minimizing friction when inserting medical devices and in contact with skin. In addition it can be applied to various application fields such as human implantable stents and catheters, and is expected to improve the performance and lifespan of medical parts.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.22 no.6
• /
• pp.542-549
• /
• 2021

The purpose of this study was to provide an economical and easy preparation method for recombinant human epidermal growth factor (rhEGF) without the need for an expensive enzyme to cleave the fusion part. However, the N-terminal fusion part is still useful for affinity chromatography. The hEGF is an important hormone in cell growth and proliferation in humans, and many studies on the expression and purification of this protein have been reported. In the present study, the hEGF gene was designed to be optimized with the E. coli codon usage preference and to contain Asn-Gly at the N-terminus of the protein. The gene was inserted into pRSET_A, an E. coli expression vector, and transformed into E. coli BL21 (DE3). The recombinant fusion protein was successfully co-expressed with pG-Tf2, a chaperone vector, in E. coli and purified by Ni-NTA column chromatography. The rhEGF was then released by hydroxylamine treatment and confirmed by SDS-PAGE. ELISA analysis showed that the activity of the free rhEGF was more than 92% similar to that of commercial EGF. The biological activity of the rhEGF was confirmed by a cell proliferation test with human skin fibroblasts.

• Journal of Korean Society of Forest Science
• /
• v.100 no.2
• /
• pp.136-141
• /
• 2011

This study was conducted to confirm the application as ingredients of cosmetics through an examination of the function for anti-cancer and anti-microbial of the fraction isolated from Pyrus ussuriensis leaves. The dried leaf of P. ussuriensis were extracted with acetone-$H_{2}O$ (6:4, v/v), concentrated and fractionated with the upper layer of acetone on a separatory funnel. Each fraction was freeze dried, then a portion of acetone soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents and also used the MIC-gel using a series of aqueous methanol as developing solvent. The isolated compounds were identified by silica-gel TLC. The growth inhibition activity was measured using the MTT assay by the mouse meltioma (B16F10) cell. The cancer cell growth inhibition rate of fractions isolated from P. ussuriensis leaf was 80%. In anti-microbial activity test, the fraction of P. ussuriensis with 0.25 mg/disc resulted in the clear zone of 1.3 cm and 2 cm for Staphylococcus aureus and S. epidermidis of gram positive bacillus, respectively. In Escherichia coli of gram negative bacillus, the fraction with 0.5 mg/disc resulted in the clear zone of 1.1 cm~1.5 cm each fraction. From these results, we confirmed that acetate fraction of P. ussuriensis has a great potential as a natural ingredients with a anti-cancer and anti-microbial source.

• Korean Journal of Veterinary Service
• /
• v.43 no.4
• /
• pp.201-209
• /
• 2020

In Korea, bovine tuberculosis (bTB) is a representative zoonotic disease that causes considerable economic loss. In determining the positive bTB, the ELISA method for examining the amount of interferon-gamma (IFN-γ) is included in Korea's diagnostic standard method. Recently, commercially available BIONOTE TB-Feron ELISA Plus (TB-Feron Plus) that detects IFN-γ has been introduced. However, since the scientific basis for the performance is limited, we evaluated performance by comparing it with the results of another IFN-γ ELISA assay kit (BOVIGAM®) certified by Office International des Epizooties. In our research, 42 positive blood samples preliminarily tested with a tuberculin skin test and/or BOVIGAM® and 54 negative blood samples collected from three bTB free farms were subjected to IFN-γ assay using the TB-Feron Plus and the BOVIGAM®, respectively. The result shows that the sensitivity, specificity and accuracy were 81.0% (34/42), 100% (54/54), 91.7% (88/96) in TB-Feron Plus kit and 78.6% (33/42), 100% (54/54), 90.6% (87/96) in BOVIGAM® kit, respectively. Moreover, the overall accordance percentage of the two kits was 99.0% (95/96) and there was almost perfect agreement between two assays (Kappa=0.977, P<0.0001). Furthermore, additional studies confirmed that elevated lymphocyte numbers in blood did not interfere with the results of the TB-Feron Plus kit. And, delayed time from sampling to culture decreased the optical density (OD) value. Therefore, we concluded that the TB-Feron Plus kit was not inferior to BOVIGAM® in performance. High lymphocyte numbers in blood did not impact on TB-Feron Plus results, while delayed time before culture interfered with OD value.

• Composites Research
• /
• v.35 no.4
• /
• pp.269-276
• /
• 2022

In this paper, a composite stiffened panel was fabricated using a three-dimensional weaving method that can reduce the risk of delamination, and mechanical properties such as buckling load and natural frequency were investigated. The preform of the stringer and skin of the stiffened panel were fabricated in one piece using T800 grade carbon fiber and then, resin (EP2400) was injected into the preform. The compression test and natural frequency measurement were performed for the stiffened panel, and the results were compared with the finite element analyses. In order to compare the performance of 3D weaving structures, the stiffened panels with the same configuration were fabricated using UD and 2D plain weave (fabric) prepregs. Compared to the tested buckling load of the 3D woven panel, the buckling loads of the stiffened panels of UD prepreg and 2D plain weave exhibited +20% and -3% differences, respectively. From this study, it was confirmed that the buckling load of the stiffened panel manufactured by 3D weaving method was lower than that of the UD prepreg panel, but showed a slightly higher value than that of the 2D plain weave panel.

• Journal of the Korean Geotechnical Society
• /
• v.38 no.5
• /
• pp.5-17
• /
• 2022

A pile is a type of medium for constructing superstructures in weak geotechnical conditions. A pretensioned spun high-strength concrete (PHC) pile is composed of high-strength concrete with a specified strength greater than 80 MPa. Therefore, it has advantages in resistance to axial and bending moments and quality control and management since it is manufactured in a factory. However, the skin friction of a pile, which accounts for a large portion of the pile bearing capacity, is only approximated using empirical equations or standard penetration test (SPT) N-values. Particularly, there are some poor research results on the pile-soil interface under the seismic loads in Korea. Additionally, some studies do not consider geoenvironmental elements, such as groundwater pH values. This study performs sets of cyclic simple shear tests using submerged concrete specimens for 1 month to consider pH values of groundwater and clay specimens composed of kaolinite to generate a pile-soil interface. 0.2 and 0.4 MPa of normal stress conditions are considered in the case of pH values. The disturbed state concept is employed to express the dynamic behavior of the interface, and the disturbed function parameters are newly suggested. Consequently, the largest disturbance increase under basic conditions is observed, and an early approach to the failure under low normal stress conditions is presented. The disturbance function parameters are also suggested to express this disposition quantitatively.

• The Journal of Korean Medicine
• /
• v.43 no.1
• /
• pp.6-17
• /
• 2022

Objectives: This study describes the implementation of a training course that aimed to increase the competency of undergraduate students of Korean medicine at a single college to obtain informed consent and perform bee venom acupuncture using an objective structured clinical examination (OSCE). Methods: We developed and implemented a short training course for 111 fourth-year students of Korean medicine during their clinical rotation at the Department of Acupuncture and Moxibustion Medicine. Lectures were conducted on the effects of and hypersensitivity reactions to bee venom acupuncture, obtaining informed consent, performing skin test and bee venom acupuncture. Due to the COVID-19 pandemic, some students were offered online lectures, and some could not experience the performance of OSCE in person. A survey with 16 closed and three open questions was used to investigate self-efficacy, opinions about the online lectures, and the OSCE. Results: Out of 111 questionnaires, 104 were completed (94%). Students were generally satisfied with the online lectures and OSCE. For the subjective questionnaire about what was helpful in the training of bee venom acupuncture techniques and OSCE, the authenticity of the teaching method was recorded the highest. Conclusions: This study showed that online lectures were effective in teaching clinical skills of Korean Medicine. It is necessary to produce standard educational materials for clinical skills of Korean Medicine, and studies to analyze the reliability and validity of OSCE are needed.