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http://dx.doi.org/10.14578/jkfs.2011.100.2.2

Anti-cancer and Anti-microbial Effect of the Fraction Isolated from Pyrus ussuriensis Leaves  

Lee, Chang-Eon (Department of Cosmeceutical Science, Daegu Haany University)
Kim, Young-Hun (Skin Science R&D Center, Mediway Korea Co., Ltd.)
Lee, Byung-Guen (Department of Forest Resources, Yeungnam University)
Lee, Do-Hyung (Department of Forest Resources, Yeungnam University)
Publication Information
Journal of Korean Society of Forest Science / v.100, no.2, 2011 , pp. 136-141 More about this Journal
Abstract
This study was conducted to confirm the application as ingredients of cosmetics through an examination of the function for anti-cancer and anti-microbial of the fraction isolated from Pyrus ussuriensis leaves. The dried leaf of P. ussuriensis were extracted with acetone-$H_{2}O$ (6:4, v/v), concentrated and fractionated with the upper layer of acetone on a separatory funnel. Each fraction was freeze dried, then a portion of acetone soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents and also used the MIC-gel using a series of aqueous methanol as developing solvent. The isolated compounds were identified by silica-gel TLC. The growth inhibition activity was measured using the MTT assay by the mouse meltioma (B16F10) cell. The cancer cell growth inhibition rate of fractions isolated from P. ussuriensis leaf was 80%. In anti-microbial activity test, the fraction of P. ussuriensis with 0.25 mg/disc resulted in the clear zone of 1.3 cm and 2 cm for Staphylococcus aureus and S. epidermidis of gram positive bacillus, respectively. In Escherichia coli of gram negative bacillus, the fraction with 0.5 mg/disc resulted in the clear zone of 1.1 cm~1.5 cm each fraction. From these results, we confirmed that acetate fraction of P. ussuriensis has a great potential as a natural ingredients with a anti-cancer and anti-microbial source.
Keywords
Pyrus ussuriensis; Anti-cancer; anti-microbial; gram negative bacillus; gram positive bacillus;
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Times Cited By KSCI : 5  (Citation Analysis)
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