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http://dx.doi.org/10.7853/kjvs.2020.43.4.201

Performance comparison and evaluation of interferon-gamma assay kit for bovine tuberculosis diagnosis  

Hong, Leegon (College of Veterinary Medicine, Seoul National University)
Choi, Woojae (College of Veterinary Medicine, Seoul National University)
Ro, Younghye (College of Veterinary Medicine, Seoul National University)
Ahn, Sunmin (College of Veterinary Medicine, Seoul National University)
Kim, Eunkyung (Farm Animal Clinical Training and Research Center, Seoul National University)
Choe, Eunhee (Farm Animal Clinical Training and Research Center, Seoul National University)
Kim, Danil (College of Veterinary Medicine, Seoul National University)
Publication Information
Korean Journal of Veterinary Service / v.43, no.4, 2020 , pp. 201-209 More about this Journal
Abstract
In Korea, bovine tuberculosis (bTB) is a representative zoonotic disease that causes considerable economic loss. In determining the positive bTB, the ELISA method for examining the amount of interferon-gamma (IFN-γ) is included in Korea's diagnostic standard method. Recently, commercially available BIONOTE TB-Feron ELISA Plus (TB-Feron Plus) that detects IFN-γ has been introduced. However, since the scientific basis for the performance is limited, we evaluated performance by comparing it with the results of another IFN-γ ELISA assay kit (BOVIGAM®) certified by Office International des Epizooties. In our research, 42 positive blood samples preliminarily tested with a tuberculin skin test and/or BOVIGAM® and 54 negative blood samples collected from three bTB free farms were subjected to IFN-γ assay using the TB-Feron Plus and the BOVIGAM®, respectively. The result shows that the sensitivity, specificity and accuracy were 81.0% (34/42), 100% (54/54), 91.7% (88/96) in TB-Feron Plus kit and 78.6% (33/42), 100% (54/54), 90.6% (87/96) in BOVIGAM® kit, respectively. Moreover, the overall accordance percentage of the two kits was 99.0% (95/96) and there was almost perfect agreement between two assays (Kappa=0.977, P<0.0001). Furthermore, additional studies confirmed that elevated lymphocyte numbers in blood did not interfere with the results of the TB-Feron Plus kit. And, delayed time from sampling to culture decreased the optical density (OD) value. Therefore, we concluded that the TB-Feron Plus kit was not inferior to BOVIGAM® in performance. High lymphocyte numbers in blood did not impact on TB-Feron Plus results, while delayed time before culture interfered with OD value.
Keywords
Bovine tuberculosis; Gamma-interferon; Diagnosis; Delayed time; Lymphocyte;
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