• Korean Journal of Plant Resources
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• v.13 no.1
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• pp.48-53
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• 2000

Lacquer tree can be proliferated by root or stem cutting, and seed. In case of proliferation by seed, however, the germination rate is very low. Thus, the present study was carried out to obtain aspceptic lacquer plant in vitro from seed because natural tissue culture was highly defiled by unknown fungi and bacteria. First seed grading on distilled water was 50.7% and second seed grading was 20.8% after 98% sulfuric acid treatment for 2 hours. Removal of inner seed coat was higher with 32.4% than non-removal of outer seed coat and removal outer seed coat in rooting rate. In germination rate according to pre-treatment, growth regulators were not effective at all, but sulfuric acid was effective a little with 3%. Removal outer seed coat was increased about 4%, that germinated about 10% in MS medium supplemented with 1.0mg/L BA and 0.05mg/L NAA, 1.0mg/L BA. Lacquer tree seeds germinated after 10 days in MS medium, and aspceptic seedling of lacquer tree were obtained after 3 weeks in vitro. Germination rate, however, was lower about 10%.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.341-345
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• 2008

Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.

• Current Research on Agriculture and Life Sciences
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• v.28
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• pp.31-38
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• 2010

Extraction efficiency of colorants from red bean and black bean seed coat and its storage stability were examined according to the various extracting and storage conditions in this study. The obtained results were as follows ; The absorbance values of extracted colorants were increased with increasing extract time and temperature. pH 4 extracting was most efficient among the various pH conditions. The absorbance values of extracted colorant from red bean were increased with increasing extract time and temperature, when the extraction process was kept at $30^{\circ}C$ and $50^{\circ}C$, but it were decreased with increasing extract time at $70^{\circ}C$. In the case of black bean, it were also increased with increasing extract time and temperature. pH 5 extract was most efficient among the various pH conditions. In juice extractor system from red bean, the pH 5 of the pre-treatment was more efficient than the distilled water of pre-treatment. In black beans of the short-term pre-treatment with distilled water, the colorant was extracted better than at pH 5, but above 4 hours pre-treatment was decrease in pH 5. The Methionine added as a stabilizer gave the more effect to the stability of colorant from red bean the more Methionine addition. In the case of black bean 10mmol addition of methionine was more efficient than 20mmol.

• Korean Journal of Plant Resources
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• v.30 no.1
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• pp.50-57
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• 2017

This study aimed to investigate the morphological characteristics and optimal germination conditions in seeds of Arabis pendula L., a traditionally edible and medicinal plant. The external seed shape was circular-obovate with narrow wings and dark brown. The seed length and width were 1.25 mm and 1.47 mm, respectively. The seeds were exalbuminous and the embryo was a bent type. Seed germination was the highest (49.7%) at $20^{\circ}C$ under dark conditions among the various temperature and light conditions applied. However, under the dark condition, the seedling was weak, overgrown, and the cotyledons were small and folded. To improve the germination and growth of seedlings, the seeds were pre-treated with $GA_3$ solutions of varying concentrations (0, 200, 500, and 1,000 mg/L). The seed germination and seedling growth were effectively improved by $GA_3$ pre-treatment. The germination rate was the highest (97.3%), mean germination time was the shortest (8.1 days), and a vigorous growth of seedlings was observed upon pre-soaking the seeds in 500 mg/L $GA_3$ solution. In conclusion, the best method for germination was pre-soaking in 500 mg/L $GA_3$ ($4^{\circ}C$, dark, 24 h) and incubating the seeds at $20^{\circ}C$ for 15 days.

• KSBB Journal
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• v.28 no.5
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• pp.287-294
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• 2013

Stained and discolored hair will be damaged by the shampooing, daily UV disposal, and the use of hair dryer. Thus many studies about the effect of various natural substances on the re-secure the skin and scalp are recently reported. This study was carried out to investigate the effect of green tea (Camelloia sinensis) seed oil on colored (dyed) and decolored (bleached) hair. The beneficial effects of green tea seed oil are already well known, but little research has been done about the hair treatment and fade-resistant effect. Dyed and bleached hair was pretreated with green tea seed oil to determine the tensile strength and elongation of the hair, to analyze the hair surface using SEM, and to compare the color fade using spectrocolormeter. The results showed that the tensile strength increased with green tea seed oil pretreatment samples for virgin, dyed, and bleached hairs. Elongation showed the reverse results showing the presence of hair treatment effect. The results of the surface pre-treatment in all groups analyzed by SEM, the hair cuticle became sharper, so coating effect were identified with all samples. The value of the $L^*$, $a^*$, $b^*$ decreased with washed hairs damaged by UV irradiation and the values were decreased also in dyed and bleached hair. In summary, green tea seed oil prevent reinjury to the heat and UV rays for colored and decolored hairs. Cosmetic practice effects of the oil were identified in the field to be appropriate to the customer's skin and scalp that natural cosmetic oils would like to offer.

• Asian Journal of Turfgrass Science
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• v.12 no.4
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• pp.195-202
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• 1998

The development of a protocol for high efficiency of embryogenic callus separation, maintenance and plant regeneration from the seeds of zoysiagrass cv. Zenith was studied. Embryogenic callus ratio is absolutely determined by genotype, but by adding high concentration of copper into medium, changing light condition and maintaining callus on initial induction medium for 8∼10 weeks, embryogenic callus can be easily distinguished and its growth can be promoted. There were significant differences among selected callus lines (each from one seed) according to their growth rates and regeneration percentages. Callus pre-treatment with activated charcoal inhibited callus growth, increased the level of precocious germination during culture and promoted shoot cluster formation after transfer to regeneration medium. For long-term callus maintenance, N6AA medium was better than MS medium, because the former inhibited non-embryogenic callus formation and kept vigor of embryogenic callus. The best callus lines Z-(5) has been successfully used for transformation and somaclonal variation selection in our laboratory.

• Journal of Korean Society of Forest Science
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• v.108 no.4
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• pp.531-539
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• 2019

This study assessed the vitality of Carpinus laxiflora seeds stored for more than 15 years in order to discover optimal pre-treatment conditions for removingthe causes of seed dormancy and improving revitalization rate. Seeds were collected in October 2000 and stored at -18℃ for 16 years. Experiments to assess the revitalization ability of the seeds were performed under the following conditions: a controlled environment; in warm stratification (30 days at 23℃); in cold stratification (30 days at 4℃ andfor 30, 45, 60, and 120 days); gibberellin (GA₃) treatment (24 hours per day in GA₃ solutions of 100, 500, 1000, 2000, and 3000 mg·L^-1); and both cold stratification and GA₃ treatment (30 days at 4℃, then in a GA₃ solution of 100, 500, and 1000 mg·L^-1 for 2 hours). The average germination percentage(GP) of untreated seeds was 2%, and the average GP of warm-stratification seeds was 10%. Cold-stratification seeds had the highest GP at 81% for the 45-day process, while the 120-day cold-stratification seeds had the lowest GP at 67.3%. The average GP of seeds treated with GA₃ ranged from 77% (100 mg·L^-1) to 99% (1000 mg·L^-1), indicatingsignificant differences between the treatment concentrations. The treatment effect of GA₃ was highest at 500 mg·L^-1 and 1000 mg·L^-1, and lowest at 100 mg·L^-1. The average GP of seeds treated with GA₃ following cold stratification was 68%, which was lower than the cold stratification-only (73.2%) and GA₃-only (88.4%) treatments. A comprehensive comparison of the seed germination characteristics according to the four treatments determined that a GA₃ 500 mg·L^-1 pre-treatment, with the highest average GP, was ideally suited to the revitalization of long-term stored C. laxiflora seeds. Consequently, C. laxiflora stored at -18℃ for 16 years indicated strong vitality and could be regenerated by proper pre-treatment.

• Progress in Superconductivity and Cryogenics
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• v.22 no.1
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• pp.1-6
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• 2020

This is a parameter study for the direct fabrication of a large grain YBCO bulk superconductors using Y₂O₃, BaCO₃ and CuO powders without any grinding step. The cracks, which have been formed due to volume contraction during calcination step, have been prevented by controlling the heating rate at 930~950 ℃. It has been observed that multi-grain growth has occurred due to the dissolution of Sm123 seed due to the retention of carbon in Ba-Cu-O melt. In order to accelerate the carbon release in prior calcination heat treatment, the reduction of pellet thickness and the drilling of artificial holes have been applied. Single-grain YBCO bulk superconductor has been successfully fabricated by stacking multiple thin slab. However, the crack formation has been rather prominent for the compact with artificial holes. The use of buffer pellet, which is supposed to act as diffusion barrier, has prevented the dissolution of Sm123 seed crystal and has led to the growth of single grain of high content of carbon containing specimen.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.4
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• pp.316-320
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• 2015

In order to improve the transformation efficiency of zoysiagrass by increasing the frequency of callus formation from mature seeds and plant regeneration, the effect of pre-treatment with sea sand was examined. Mature zoysiagrass seeds were given 10 min of swelling time before sea sand treatment using a sea sand and seed ratio of 1 : 1 and a vortex shaking speed of 6 (1,000 rpm) for 10 min. The seeds showed increased callus formation that was more than 2 times the rate in the control. In addition, plant regeneration efficiency was also increased when embryogenic callus induced from sea sand-treated mature seeds was cultured in regeneration medium. These results will be very helpful for improving the genetic transformation frequency of zoysiagrass, a recalcitrant monocot grass.

• Korean Journal of Plant Resources
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• v.26 no.1
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• pp.75-83
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• 2013

The present study was examined seed germination and growth characteristics of Cynanchum wilfordii under the following conditions such as soaking temperature, concentrations of $GA_3$, trays and soil types, and shading conditions. In seed pre-treatment, germination rate was the highest at 93% when soaked in 100 ppm $GA_3$ at $20^{\circ}C$ and seedling growth was best in 50 plug cell tray. Physiological quality was best in the condition in which seeds were treated with 10 ppm $GA_3$ at $20^{\circ}C$. Taking into consideration the seedling growth, their physiological quality as well as economical aspects, seedlings with plant height (over 10 cm) and root length (over 10 cm) were grown vigorously 30-45 days after the seeds were sown in mid-April in TKS, TKS+perlite and TKS+rice hull of 128 or 200 plug cell tray.