• Journal of fish pathology
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• v.27 no.2
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• pp.133-137
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• 2014

We examined the cause of a disease outbreak in rainbow trout Oncorhynchus mykiss, which were adapting to a seawater in an aquaculture farm in Jeju on April, 2013. Most of the diseased fish showed a severe ulcer on the skin, enlarged spleen, expanded stomach and hemorrhage of abdominal and pyloric region. Although no parasites, fungi or viruses were isolated from diseased fish, over 200 same bacterial colonies were isolated from liver, spleen and kidney. Nucleotide sequences of the 16S rDNA gene of the bacterium in this study showed 100% identity with Vibrio anguillarum. This study is the first report of V. anguillarum infection in rainbow trout during sea adaption in Korea.

• Journal of fish pathology
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• v.18 no.2
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• pp.187-195
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• 2005

Effluent of aquaculture industry has caused a growing concern regarding its environmental impact. We assessed the use of flounder farming sewage as supplement of diet, to minimize the impact of aquaculture on the environment or also establish the technique for the recycling of effluent sediment derived from land-based seawater fish farm. In order to investigate the effects of a fermented aquaculture waste on biochemical responses of olive flounder (Paralichthys olivaceus), fermented products of aquaculture wastes were used as test compounds that cause hepatic and renal stress through the induction of oxidative stress in liver and kidney. Hepatosomatic index (HSI), glutathione content and glutathione dependent enzyme were not significantly different and no correlation was found within the different types of fermentation condition or supplement concentration, except for significant increases in 50% fermentation group and 50% concentration group in case of glutathione peroxidase activity and HSI value, respectively. These results showed addition of fermented aquaculture sewage may be an economic artificial sources of diet for fish aquaculture practices without affecting the function and safety in view of biochemical examination.

• Korean Journal of Environmental Biology
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• v.36 no.1
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• pp.90-97
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• 2018

This study assessed the effect of ozone to control pathogenic bacteria in inlet water flowing to flounder farms, establishing operational parameters of ozonation at seawater conditions. Hydraulic retention time in a reaction pipeline after ozonation was fixed at 3 minutes in a flow through system. Concentrations of total residual oxidant (TRO) by ozonation were measured according to different ozonation intensities. The oxidant reduction potential (ORP), which is indirect but enables real-time measurement, was measured in relation to TRO values. TRO values were $0.01{\pm}0.01mg\;L^{-1}$ at an ORP range of 320-410 mV, $0.07{\pm}0.02mg\;L^{-1}$ at 600 mV, and $0.16{\pm}0.03mg\;L^{-1}$ at 700 mV. A heterotrophic marine bacteria colony was reduced by 80.6-97.9%, showing the suppression effect of ozonation on total bacteria in inlet water. At an ORP range of 400-500 mV, colonies of heterotrophic marine bacteria, Vibrio spp., and gram negative bacteria were significantly reduced in outlet water from a culture tank with ongrowing flounder (230 g) at a stocking density of $8kg\;m^{-2}$. Especially, Vibrio spp. and gram negative bacteria were seldom found at 400-500 mV. The daily feeding rate was from over 0.7% to total body weight at 300-500 mV, showing better performance than that in the control. The pathogenic bacteria entering the flounder farm were effectively removed when the ORP range to 400 mV or less.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.5
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• pp.324-329
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• 2008

Vibrio parahaemolyticus species, which cause acute gastroenteritis in humans, were isolated from farmed fish and seawater and their antimicrobial-resistance pattern and factor were investigated. They exhibited the highest resistance to ampicillin (88.9%), followed by trimethoprim (51.9%) and rifampin (22.2%). The relatively high resistance to trimethoprim was unexpected because trimethoprim was not commonly used in fish farming in Korea. R plasmid related resistance was identified by the treatment of novobiocin (7 ug/mL) and it was named as pVPBW1. A putative trimethoprim resistance gene in 2.0 kb fragment of pVPBW1 was also confirmed.

• Journal of Aquaculture
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• v.16 no.1
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• pp.44-50
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• 2003

This study aimed to develop the methods of growing Enteromorpha prolifera natural seedlings in its natural habitat and artificial indoor seedlings by inducing spore release. Likewise, the study examined the possibility of mass production by developing cultivation techniques with cultivating examination. The natural seedling of E.prolifera thrived in a sea area composed of sand and mud, which Is its natural habitat. Growing of this alga on the seedling frame 20 cm high from the bottom at the intertidal zone in summer and 40 cm high in fall was found to be very effective. However, enabling the best attachment rate for artificial indoor seedling requires inducing spore release after drying the mature thalli in a dark place fur about 12∼24 hours and setting seedling nets in a dark water tank (spore solution) for 24 hours. Breeding E.prolifera in a pole-system farm is best done in shallow sea areas with mud or mud and sand geological feature. However, floating-system lam is better for deep-sea areas with fast current. Ideal farming places are sea areas with plenty of nutritional salt and safe places that protect the lam facilities against billows. Furthermore, an exposure method on seawater surface to produce larger output should be used.

• Korean Journal of Ichthyology
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• v.30 no.3
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• pp.131-136
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• 2018

Decrease in seawater temperature during winter is one of the most important and serious issues confronted by fish net-cage aquaculture farms. This can become the cause of the manifestation of diseases and ensuing mass mortality. The present study was conducted to assess the survival rate, the range of limited low-temperature, the response of oxidative stress in the blood of red sea bream Pagrus major with decrease of water temperature. Low-lethal temperature for 7 days of P. major ($7day-LT_{50}$) was $6.54^{\circ}C$ (confidence limit: $6.31{\sim}6.71^{\circ}C$). Oxygen consumption rate was decreased with lower temperature. It showed the minimum value at the range of low-lethal temperature. Osmolality at $5^{\circ}C$ and $6^{\circ}C$ experimental group was higher significantly than control group. SOD (superoxide dismutase) activity was increased significantly at $5^{\circ}C$ experimental group compared to control group. This study data will be used to determine the appropriate area for aquaculture of red sea bream. It also manage fish net-cage farm to cope with the mass mortality occurring frequently during winter season.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.5
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• pp.735-745
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• 2022

In this study, a health assessment was conducted using the Benthic Health Index (BHI) to assess fish cage farms, where a fishery environment assessment was also performed. A total of 43 farms were evaluated located in the East Sea, West Sea, and South Sea in Korea. The results of the BHI health evaluation included 8 grade 1 farms, 4 grade 2, 12 grade 3, and 19 grade 4. The grade 1 farms included sandy sediment farms and those with low intensity aquaculture, while the grade 2 farms included those located in areas with active seawater circulation. The fish cage farms belonging to grade 3 and 4 included the majority of farms with high-intensity aquaculture activities. There was no significant difference in total organic carbon between grade 3 and 4 farms, but the results of polychaete community analysis show that organic matter concentration was significantly higher in grade 4 farms.

• Journal of Aquaculture
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• v.4 no.2
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• pp.111-128
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• 1991

In puan area the environmental surveys were carried out at two farms of hard clam, Meretrix lusoria from April 1987 to November 1978 in order to know heather the farm environments could be rehabilitated for the cultivation of hard clam or not. The range of temperature of surface seawater was $10.7{\~}27.4^{\circ}C$, pH $7.6{\~}8.2$, salinity $22.3{\~}30.3$ ppt, COD $0.20{\~}4.71\;mg/{\ell}$, sulfide $0.04{\~}0.22\;{\mu}g-at./{\ell}$, suspended solid $34.8{\~}199.3\;mg/{\ell}$ chlorophyll a $3.71{\~}49.02\;mg/m^3$, TIN $2.01{\~}24.47\;{\mu}g-a5./{\ell}$, phosphate $0.60{\~}11.03\;{\mu}g-at./{\ell}$ and silicate $4.04{\~}476.36\;{\mu}g-at./{\ell}$. The range of temperature of substratum (bottom soil) was $14.2{\~}29.7^{\circ}C$, pH $8.3{\~}9.5$, water content of substratum was $0.28{\~}0.49\;mg/g$ dried mud, COD $2.80{\~}50.94\;mg/g$ dried mud, total organic matter $1.05{\~}1.97\%$ concentration of total Kjedhal nitrogen $31.9{\~}194.9\;{\mu}g./{\ell}$ dried mud, and sulfide $0.032{\~}0.133\;mg/g$ dried mud. Fine sand was dominant ranging over $92{\~}95\%$ and silt and clay was $2.8{\~}8.1\%$ of the composition of substratum. Some residual agricultural chemicals, ${\alpha},\;{\beta},\;{\gamma}$-BHC, heptachlor, heptachlor-epoxide, aldrin, DDE, DDT and dieldrin were detected in hard clams collected from Puan areas. Especialy, more chemical were detected during the period of rainfalls. From above results, it is considered that the hard clam frams were not yet recovered from deteriorated conditions for aquaculture.

• Korean Journal of Ecology and Environment
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• v.55 no.2
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• pp.99-110
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• 2022

We investigated physicochemical properties and isotopic compositions of organic matter (δ¹³C_TOC and δ ¹⁵N_TN) in the old fish farming (OFF) site after the cessation of aquaculture farming. Based on this approach, our objective is to determine the organic matter origin and their relative contributions preserved at sediments of fish farming. Temporal and spatial distribution of particulate and sinking organic matter(OFF sites: 2.0 to 3.3 mg L^-1 for particulate matter concentration, 18.8 to 246.6 g m^-2 day^-1 for sinking organic matter rate, control sites: 2.0 to 3.5 mg L^-1 for particulate matter concentration, 25.5 to 129.4 g m^-2 day^-1 for sinking organic matter rate) between both sites showed significant difference along seasonal precipitations. In contrast to variations of δ¹³C_TOC and δ¹⁵N_TN values at water columns, these isotopic compositions (OFF sites: -21.5‰ to -20.4‰ for δ¹³ C_TOC, 6.0‰ to 7.6‰ for δ¹⁵N_TN, control sites: -21.6‰ to -21.0‰ for δ¹³C_TOC, 6.6‰ to 8.0‰ for δ¹⁵N_TN) investigated at sediments have distinctive isotopic patterns(p<0.05) for seawater-derived nitrogen sources, indicating the increased input of aquaculture-derived sources (e.g., fish fecal). With respect to past fish farming activities, representative sources(e.g., fish fecal and algae) between both sites showed significant difference (p<0.05), confirming predominant contribution (55.9±4.6%) of fish fecal within OFF sites. Thus, our results may determine specific controlling factor for sustainable use of fish farming sites by estimating the discriminative contributions of organic matter between both sites.

• Journal of the Korean Society for Marine Environment & Energy
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• v.9 no.1
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• pp.1-13
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• 2006

A field survey on the spatio-temporal distribution characteristics and origins of organic matter in surface sediments was carried out monthly at six stations in Gamak Bay, South Korea from April 2000 to March 2002. The range of ignition loss(IL) was $4.6{\sim}11.6%(7.1{\pm}1.6%)$, while chemical oxygen demand(CODs) ranged from $12.25{\sim}99.26mgO_2/g-dry(30.98{\pm}19.09mgO_2/g-dry)$, acid volatile sulfide(AVS) went from no detection(ND)${\sim}10.29mgS/g-dry(1.02{\pm}0.58mgS/g-dry)$, and phaeopigment was $6.84{\sim}116.18{\mu}g/g-dry(23.72{\pm}21.16{\mu}g/g-dry)$. The ranges of particulate organic carbon(POC) and particulate organic nitrogen(PON) were $5.45{\sim}23.24 mgC/g-dty(10.34{\pm}4.40C\;mgC/g-dry)$ and $0.71{\sim}2.99mgN/g-dry(1.37{\pm}0.58mgN/g-dry)$, respectively. Water content was in the range of $43.1{\sim}77.6%(55.8{\pm}5.6%)$, and mud content(silt+clay) was higher than 95% at all stations. The spatial distribution of organic matter in surface sediments was greatly divided between the northwestern, central and eastern areas, southern entrance area from the distribution characteristic of their organic matters. The concentrations of almost all items were greater at the northwestern and southern entrance area than at the other areas in Gamak Bay. In particular, sedimentary pollution was very serious at the northwestern area, because the area had an excessive supply of organic matter due to aquaculture activity and the inflow of sewage from the land. These materials stayed longer because of the topographical characteristics of such as basin and the anoxic conditions in the bottom seawater environment caused by thermocline in the summer. The tendency of temporal change was most prominently in the period of high-water temperatures than low-water ones at the northwestern and southern entrance areas. On the other hand, the central and eastern areas did not show a regular trend for changing the concentrations of each item but mainly showed a higher tendency during the low-water temperatures. This was observed for all but AVS concentrations which were higher during the period of high-water temperature at all stations. Especially, the central and eastern areas showed a large temporal increase of AVS concentration during those periods of high-water temperature where the concentration of CODs was in excess of $20mgO_2/g-dry$. The results show that the organic matters in surface sediments in Gamak Bay actually originated from autochthonous organic matters with eight or less in average C/N ratio including the organic matters generated by the use of ocean, rather than terrigenous organic matters. However, the formation of autochthonous organic matter was mainly derived from detritus than living phytoplankton, indicated the results of the POC/phaeopigment ratio. In addition, the CODs/IL ratio results demonstrate that the detritus was the product of artificial activities such as dregs feeding and fecal pellets of farm organisms caused by aquaculture activities rather than the dynamic of natural ocean activities.