Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Vibrio anguillarum infection in rainbow trout Oncorhynchus mykiss during seawater adaption

해수순치 무지개송어 (Oncorhynchus mykiss)의 Vibrio anguillarum 감염증

  • Kim, Si-Woo (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Jong-Oh (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Do-Hyung (Department of Aqualife Medicine, Pukyong National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
  • 김시우 (전남대학교 수산생명의학과) ;
  • 김종오 (전남대학교 수산생명의학과) ;
  • 김위식 (전남대학교 수산생명의학과) ;
  • 김도형 (부경대학교 수산생명의학과) ;
  • 오명주 (전남대학교 수산생명의학과)
  • Received : 2014.08.04
  • Accepted : 2014.08.19
  • Published : 2014.08.30
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Abstract

We examined the cause of a disease outbreak in rainbow trout Oncorhynchus mykiss, which were adapting to a seawater in an aquaculture farm in Jeju on April, 2013. Most of the diseased fish showed a severe ulcer on the skin, enlarged spleen, expanded stomach and hemorrhage of abdominal and pyloric region. Although no parasites, fungi or viruses were isolated from diseased fish, over 200 same bacterial colonies were isolated from liver, spleen and kidney. Nucleotide sequences of the 16S rDNA gene of the bacterium in this study showed 100% identity with Vibrio anguillarum. This study is the first report of V. anguillarum infection in rainbow trout during sea adaption in Korea.

본 연구에서는 2013년 4월 제주 소재 육상수조에서 무지개송어 해수순치 과정중에 대량폐사가 발생하여 질병검사를 실시하였다. 병어의 대부분은 체표에 궤양이 형성되어 있었고, 비장 비대, 유문수 부위 및 복강 근육의 출혈 및 위속에 액성물질이 가득 차 있었다. 병원체 검사결과, 기생충, 진균, 바이러스는 검출되지 않았지만 검사 개체 5마리에서 간, 비장 및 신장조직에서 동일한 형태의 세균 colony가 시료 당 200개 이상 분리되었다. 분리세균의 16S rDNA 염기서열 분석 결과, Vibrio anguillarum과 100% (875/875 bp) 일치하였다. 이상의 결과, 무지개송어 해수 순치과정 중에 발생된 질병은 Vibrio anguillarum 감염증으로 사료되었다.

Keywords

References

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  1. 무지개송어(Onchorhynchus mykiss) 위팽창증후군의 잠재적 원인체의 분자유전학적 동정 vol.50, pp.5, 2014, https://doi.org/10.5657/kfas.2017.0624
  2. 해수 사육 무지개송어(Oncorhynchus mykiss)에서 분리된 Vibrio anguillarum의 특성 분석 vol.51, pp.3, 2014, https://doi.org/10.5657/kfas.2018.0254