Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Biochemical Responses in Olive Flounder, Paralichthys olivaceus Fed Diet Supplemented with Fermented Aquaculture Sewage

양식장 배출물 발효물의 사료첨가에 따른 넙치, Paralichthys olivaceus의 생화학적 반응

  • Jee, Jung-Hoon (Institute of Fisheries Sciences, Pukyong National University) ;
  • Moon, Sang-Wook (Technology Innovation Center, Cheju National University) ;
  • Kim, Se-Jae (Technology Innovation Center, Cheju National University) ;
  • Lee, Young-Don (Marine and Environmental Research Institute, Cheju National University) ;
  • Keum, Yoo-Hwa (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kang, Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
  • 지정훈 (부경대학교 수산과학연구소) ;
  • 문상욱 (제주대학교 지역기술혁신센터) ;
  • 김세재 (제주대학교 지역기술혁신센터) ;
  • 이영돈 (제주대학교 해양과환경연구소) ;
  • 금유화 (부경대학교 수산생명의학과) ;
  • 강주찬 (부경대학교 수산생명의학과)
  • Published : 20050800
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Abstract

Effluent of aquaculture industry has caused a growing concern regarding its environmental impact. We assessed the use of flounder farming sewage as supplement of diet, to minimize the impact of aquaculture on the environment or also establish the technique for the recycling of effluent sediment derived from land-based seawater fish farm. In order to investigate the effects of a fermented aquaculture waste on biochemical responses of olive flounder (Paralichthys olivaceus), fermented products of aquaculture wastes were used as test compounds that cause hepatic and renal stress through the induction of oxidative stress in liver and kidney. Hepatosomatic index (HSI), glutathione content and glutathione dependent enzyme were not significantly different and no correlation was found within the different types of fermentation condition or supplement concentration, except for significant increases in 50% fermentation group and 50% concentration group in case of glutathione peroxidase activity and HSI value, respectively. These results showed addition of fermented aquaculture sewage may be an economic artificial sources of diet for fish aquaculture practices without affecting the function and safety in view of biochemical examination.

양식 산업의 양적 발달에 따라, 이로 인한 배출수 문제는 환경적 측면에서 관심이 증대되고 있다. 특히, 육상 수조식 넙치 양식장에서 배출되는 배출물을 사료 첨가제로 사용하는 것은 환경오염 감소와 자원의 재활용이라는 측면에서 중요한 의미를 가진다. 따라서, 본 연구는 발효물 첨가에 따른 넙치, Paralichthys olivaceus의 간 및 신장의 생화학적 스트레스 반응을 조사하였다. 배출물 발효 조건 및 첨가 농도에 따른 간 중량지수, 글루타치온 함량 및 글루타치온 의존 효소활성 등을 조사한 결과 50% 발효구와 50% 농축구에서 대조구와 비교하여 각각 glutathione peroxidase 효소활성과 간 중량지수의 유의적 증가가 관찰되었지만, 이를 제외한 실험군 차이는 나타나지 않았다. 이러한 결과로부터 생화학적 측면에서 발효물 첨가가 간 및 신장 기능과 안전성에 영향을 주지 않는 것으로 평가되며, 양식어의 사료 첨가제로서의 환경적 측면에서 고려할 필요성이 대두된다.

Keywords

References

  1. Ackefors, H. and Enell, M.: Discharge of nutrients from Swedish fish farming to adjacent sea areas. Ambio, 19: 28-35,1990
  2. Baker, M. A., Cerniglia, G. J. and Zaman, A.: Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal. Biochem., 190: 360-365,1990 https://doi.org/10.1016/0003-2697(90)90208-Q
  3. Bell, J. G., Andron, J. W. and Cowey, C. B.: Effect of selenium deficiency on hydroperoxide-stimulated release of glutathione from isolated perfused liver of rainbow trout (Salmo gairdneri), Br. J. Nutr., 56:421-428,1986 https://doi.org/10.1079/BJN19860122
  4. Beutler, E.: Red cell metabolism. In: A Manual of Biochemical Methods. Grune and Starton, New York, 133 pp, 1984
  5. Bradford, M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Bichem., 72: 248-254,1976 https://doi.org/10.1016/0003-2697(76)90527-3
  6. Chandra, S., Ram, R. N. and Singh, I. J.: First ovarian maturity and recovery response in common carp, Cyprinus carpio after exposure to carbofuran. J. Environ. Biol. 25: 239-249, 2004
  7. DiGiulio, R. T., Habig, C. and Gallagher, E. P.: Effects of black river harbour sediments on indices of biotransformation, oxidative stress, and DNA integrity in channel catfish. Aquat. Toxicol., 26: 1-22,1993 https://doi.org/10.1016/0166-445X(93)90002-I
  8. Folmar, L. C.: Effects of chemical contaminants on blood chemistry of teleost fish: A bibliography and synopsis of selected effects. Environ. Toxicol. Chem., 12: 337-375,1993 https://doi.org/10.1897/1552-8618(1993)12[337:EOCCOB]2.0.CO;2
  9. Fournier, D., Bride, J. M., Poirie, M., Berge, J. B. and Plapp, F. W.: Insect glutathione S-trans-ferases: biochemical characteristics of the major forms of houseflies susceptible and resistant to insecticides. J. Biol. Chem., 267: 1840-1845,1992
  10. Hasspieler, B. M., Behar, J. V. and Di Giulio, R. T: Glutathione dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurius nebulosus). Ecotoxicol. Environ. Saf., 28: 82-90,1994 https://doi.org/10.1006/eesa.1994.1036
  11. Kosower, N. S. and Kosower, E. N.: The glutathione status of cells. Int. Rev. Cytol., 54: 109-159,1978 https://doi.org/10.1016/S0074-7696(08)60166-7
  12. Livingstone, D. R.: The fate of organic xenobiotics in aquatic ecosystems: quantitative and qualitative differences in biotransformation by invertebrates and fish. Comp. Biochem. Physiol. A, 120:43-49,1998 https://doi.org/10.1016/S1095-6433(98)10008-9
  13. Mayer, F L., Versteeg, D. J., McKee, M. J., Folmar, L. C, Graney, R. L., McCume, D. C. and Rattner, B. A.: Metabolic products as bio-markers. In: Biomarkers: Biochemical, physiological, and histological markers of anthropogenic stress. Huggett, R. J., Kimer-le, R. A., Mehrle, R M. and Bergman H. L. (Eds.), Lewis Publishers, Boca Raton, 5-86, 1992
  14. McDonald, D. G. and Milligan, C. L.: Chemical properties of the blood. In: Fish Physiology. Hoar, W. S., Randall, D. J. and Farrell, A. P. (Eds.), Academic Press, San Diego, 55-133, 1992
  15. MOMAF: Statistical Yearbook of Maritime Affairs and Fisheries 2004, Ministry of Maritime Affairs and Fisheries, Seoul, 7-22, 2004. (in Korean)
  16. Moon, S. W., Lee, J. B., Lee, Y. D., Kim, S. J. Kang, B. J. and Go, Y. B.: Recycling marine fish farm effluent by microorganisms. J. of Aquaculture, 15: 261-266, 2002. (in Korean)
  17. Otto, D. M. E. and Moon, T. W.: 3,3',4,4'-tetra-chlorobiphenyl effects on antioxidant enzymes and glutathione status in different tissues of rainbow trout. Parmacol. Toxicol., 77:281-287, 1995
  18. Pacheco, M. and Santos, M. A.: Biotransformation, Endocrine, and Genetic Responses of Anguilla anguilla L. to Petroleum Distillate Products and Environmentally Contaminated Waters. Ecotoxicol. Environ. Saf., 49: 64-75, 2001 https://doi.org/10.1006/eesa.2000.2025
  19. Payne, J. F., Fancey, L. L., Rahimtula, A. D. and Porter, E. L.: Review and perspective on the use of mixed-function oxygenase enzymes in biological monitoring. Comp. Biochem. Physiol. C., 86: 233-245,1987 https://doi.org/10.1016/0742-8413(87)90074-0
  20. Poels, C. L. M., Van der Gaag, M. A. and van de Kerkhoff, J. F J.: An investigation into the long-term effects of Rhine water on rainbow trout. Water Research, 14: 1029-1035, 1980 https://doi.org/10.1016/0043-1354(80)90148-7
  21. Radi, A. A. R., Hai, D. Q., Matkovics, B. and Gabrielak, T.: Comparative antioxidant enzyme study in fresh water fish with different types of feeding behavior. Comp. Biochem. Physiol. C, 81: 395-399, 1985 https://doi.org/10.1016/0742-8413(85)90026-X
  22. Rodriguez-Ariza, A., Dorado, G., Peinado, J., Pueyo, C. and Lopez-Barea, J.: Biochemical effects of environmental pollution in fishes from Spanish South-Atlantic littoral. Biochem. Soc. Trans., 19(3): 301S, 1991
  23. Rodriguez-Ariza, A., Peinado, J., Pueyo, C. and Lopez-Barea, J.: Biochemical indicators of oxidative stress in fish from polluted littoral areas. Can. J. Fish. Aquat. Sci., 50: 2568-2573,1993 https://doi.org/10.1139/f93-280
  24. Siegers, C. P.: Glutathione and glutathione dependent enzymes. Progr. Pharmacol. Clin. Pharmacol., 7: 171-180,1989
  25. Slooff, W, van Kreijl, C. F. and Baars, A. J.: Relative liver weights and xenobiotic-metaboliz-ing enzymes of fish from polluted surface waters in the Netherlands. Aquat. Toxicol., 4: 1-14, 1983 https://doi.org/10.1016/0166-445X(83)90057-7
  26. Stegeman, J. J., Brouwer, M., DiGiulio, R. T., For-lin, L., Fowler, B. A., Sanders, B. M. and Van Veld, P. A.: Molecular response to environmental contamination: Enzyme and protein systems as indicators of chemical exposure and effect. In: Biomarkers: Biochemical, physiological, and histological markers of anthropogenic stress. Huggett, R. J., Kimerle, R. A., Mehrle, P. M. and Bergman H. L. (Eds.), Lewis Publishers, Boca Raton, 235-335,1992
  27. Wu, R. S. S.: The environmental impact of marine fish culture: towards a sustainable future. Marine Pollution Bulletin, 31: 159-166, 1995 https://doi.org/10.1016/0025-326X(95)00100-2
  28. Zhang, J. E, Wang, X. R., Guo, H. Y, Wu, J. C. and Xue, Y. Q.: Effects of water-soluble fractions of diesel oil on the antioxidant defenses of the goldfish, Carassius auratus Ecotoxicol. Environ. Saf., 58: 110-116,2004 https://doi.org/10.1016/j.ecoenv.2003.08.025