• 생약학회지
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• 제42권3호
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• pp.265-270
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• 2011

Acute toxicity of a combined preparation of the standardized extracts Scutellaria baicalensis GEORGI and Salvia miltiorrhiza BUNGE in a ratio of 3:1 was examined in male and female ICR mice. Mice were treated with the test substance intragastrically at a dose of 0 mg/kg, 5 mg/kg, 50 mg/kg, 500 mg/kg or 2,000 mg/kg and observed for two weeks. No death or abnormal clinical sign was shown during the observation period. Also there were no difference in net body weight gain, organ weight, and gross pathological findings at the terminal sacrifice. The results suggested that acute oral toxicity of a combined preparation of the standardized extracts is very low at the conditions employed in this study.

• Parasites, Hosts and Diseases
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• 제49권3호
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• pp.255-263
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• 2011

The present work evaluated the effects of alcoholic extracts of salvia (Salvia officinalis), thyme (Thymus vulgaris), and 2 pure compounds (thymol and menthol) on the viability of Echinococcus granulosus protoscolices in vitro. Four different concentrations of each extract (2,500, 1,500, 1,000, and 500 ${\mu}g$/ml) and 3 different concentrations each of thymol and menthol (50, 10, and 1 ${\mu}g$/ml) were used. Concentration of 2500 ${\mu}g$/ml of both extracts showed a significant protoscolicidal activity on the 6th day. Complete loss of viability of protoscolices occurred with 500 ${\mu}g$/ml concentration of both extracts at day 6 and day 7 post-treatment (PT), respectively. Pure compounds, i.e., menthol and thymol, showed potent effects with 50 ${\mu}g$/ml concentration at day 2 and day 5 PT, respectively. These effects were compared with those of albendazole sulfoxide (800 ${\mu}g$/ml), a commonly used treatment drug for hydatidosis. Krebs-Ringer solution and the hydatid cystic fluid at a ratio of 4:1 was a good preservative solution which kept the protoscolices viable for 15 days.

• 한국식품영양과학회지
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• 제27권5호
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• pp.858-863
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• 1998

The Mixed effect of Salvia miltiorrhiza and Glycyrrhiza uralensis(SG) on kimchi fermentation was investigated by measuring changes of physicochemical, icrobiological and sensory qualities of kimchi during fermentation. The pH of SG-added kimchi was a little higher than that of control during the fermentation. Titratable acidity, viable cell of total bacteria and lactic acid bacteria in mixed medicinal herbs(SG) added kimchi were changed more slowly than those in control. The inhibitory effect of the mixture on kimchi fermentation was increased as the concentration of the mixture was increased from 1% to 5%. Total bacteria and lactic acid bacteria of 3% and 5% SG-added kimchi reduced to 1.3~2.9 and 1.2~4.0 log10 cycle after 15 days fermentatin compared to control. The changes in texture of SG-added kimchi was a higher and sour taste of SG-containing kimchi excepts of 1% SG-added kimchi was more weak than that of control. Sensory score of flavor and overall acceptability did not show any significant difference between SG-added kimchi and control during fermentation. But SG-added kimchi decreased its sensory quality by 5% the other kimch.

• Journal of Applied Biological Chemistry
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• 제64권4호
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• pp.391-397
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• 2021

Four compounds were isolated from Salvia plebeia aerial parts. Silica gel open column chromatography with a gradient elution system was used to isolate and purify these compounds. Nuclear magnetic resonance spectroscopy and mass spectroscopy were used for structural elucidation and identification, while electronic circular dichroism was used to confirm the absolute configuration. The structures were determined to be 𝛽-sitosterol (1), (-)-1S,5S,8S,10R-1-acetoxy-8-hydroxy-2-oxoeudesman-3,7(11)-dien-8,12-olide (2), ursolic acid (3), and N-methylhydroxylamine (4). Compounds 2 and 4 were isolated for the first time from this plant. Compound 2 was quantitatively analyzed via HPLC/UV. The results showed that the methanol extract of S. plebeia had a higher content of compound 2 (1.20 mg/g) than the ethanol extract (0.55 mg/g). This study could be used as a preliminary step in conducting HPLC/UV analysis of sesquiterpenoids in S. plebeia extract to assess their bioavailability and potency.

• 생약학회지
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• 제49권4호
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• pp.322-327
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• 2018

Methanol extract of Salvia miltiorrhiza Bunge (Labiatae) root was investigated to research the anti-oxidative activity, by using a Caenorhabditis elegans model system. The methanol extract of this plant showed significant DPPH radical scavenging and superoxide quenching activities. Ethyl acetate soluble fraction of the methanol extract that showed the most potent DPPH radical scavenging and superoxide quenching activities. The ethyl acetate fraction was tested on its activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance in C. elegans. Furthermore, in order to see if regulation of stress-response genes is responsible for the increased stress tolerance of the ethyl acetate fraction treated C. elegans, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of S. miltiorrhiza root increased the catalase and SOD activities in a dose-dependent manner in C. elegans. Besides, the ethyl acetate fraction-treated CF1553 worms showed higher SOD-3::GFP intensity than the non-treated ones.

• BMB Reports
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• 제53권12호
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• pp.652-657
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• 2020

Danshen (Salvia miltiorrhiza) is a traditional medicinal plant widely used in Asian countries for its pharmacological activities (e.g., amelioration of cardiovascular diseases). In this study, we investigated the anti-atherosclerotic activity of raw danshen root extract prepared using high hydrostatic pressure (HHP) at 550 MPa for 5 min and hot water extraction. This method was useful for elimination of bacteria from cultured danshen plants and for better extraction yield of active principles. The HHP-treated danshen extract (HDE) inhibited proliferation of human umbilical vein endothelial cells (HUVECs) and induced autophagy that was assessed by LC3 conversion and p62 degradation. HDE suppressed foam cell formation in oxLDL-induced RAW264.7 macrophages; lysosomal activity simultaneously increased, measured by acridine orange staining. HDE also reduced atherosclerotic plaque development in vivo in apolipoprotein E knock-out (ApoE-/-) mice fed a high cholesterol diet. Taken together, these results indicated that HDE exhibited anti-atherosclerotic activity via autophagy induction.

• 생물환경조절학회지
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• 제26권3호
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• pp.208-214
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• 2017

본 연구에서는 인공광 광질에 따른 곰보배추의 생육, 엽형 및 항산화능을 비교하였다. 정식 후 56일째에 측정된 엽장, 엽폭 및 엽수는 적색 LED(R)와 대조구인 형광등(FL) 처리구에서 유의적으로 높았고 혼합광(RB2:1)과 백색 LED(W) 처리구에서 낮았다. 엽형지수(엽장/엽폭)도 적색 LED(R)와 대조구인 형광등(FL) 처리구에서 유의적으로 높았고 혼합광(RB2:1)과 백색 LED(W) 처리구에서 낮았다. 지상부 생체중, 건물중 및 엽면적 모두 적색 LED(R) 처리구에서 가장 높았고 형광등(FL)과 청색 LED(B) 처리구가 그 다음으로 높게 나타났으며 혼합광(RB2:1)과 백색 LED(W) 처리구에서 낮았다. 잎의 수하현상은 정식 21일 이후부터 적색 LED(R)와 혼합광(RB2:1) 처리구에서만 발생하였다. 엽록소 함량은 적색 LED(R) 처리구에서 가장 낮았다. 비엽중은 청색 LED(B) 처리구에서 가장 높았고 형광등(FL) 처리구에서 가장 낮았다. DPPH free radical 소거활성은 처리 간 유의차가 없었다. 위의 결과들을 종합하여 볼 때 형광등이 곰보배추를 실내에서 인공으로 재배 시 적합한 광원으로 판단된다.

• 한국식품영양과학회지
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• 제41권7호
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• pp.888-894
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• 2012

본 연구에서는 배암차즈기의 항염증 효과를 알아보기 위하여 LPS 자극에 의해 활성화된 RAW264.7 대식세포에서 NO, PGE2 활성, iNOS, COX-2와 HO-1의 발현의 변화를 측정하였다. 연구결과 RAW264.7 대식세포에 LPS를 처리하여 증가되어진 NO의 함량이 배암차즈기 잎 추출물을 처리한 결과 농도 의존적으로 감소되어짐을 확인할 수 있었으며, 또한 배암차즈기 잎 추출물은 NO의 생성에 관여하는 iNOS 단백질 발현과 전사단계의 iNOS의 mRNA 발현을 농도 의존적으로 저해하는 것을 알 수 있었다. 이 결과로 배암차즈기 잎 추출물이 전사단계에서 저해 활성을 나타낸다는 것을 보여주었다. NO 이외의 pro-inflammatory cytokine인 PGE2 또한 배암차즈기 추출물에 의해 농도 의존적으로 감소되었으며 PGE2 생성에 관여하는 효소인 COX-2의 발현 또한 저해하는 활성을 나타내었다. 또한, 배암차즈기 잎 추출물은 HO-1 단백질 발현을 농도 의존적으로 유도하였다. 본 연구 결과 배암차즈기 추출물은 염증을 일으키는 주요인자인 NO, PGE2를 저해하였고, iNOS, COX-2의 발현, iNOS의 mRNA 발현을 억제하여 항염증 효과에 우수한 효과를 나타내었다. 이는 산화적 손상으로부터 세포 보호 방어기작에 관여하는 HO-1의 발현을 증가시킴에 따라 항염증에 우수한 효과를 보였으며 항염증 연구의 기초 자료로 활용될 것으로 예상된다.

• 원예과학기술지
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• 제35권3호
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• pp.289-299
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• 2017

The objective of this study was to determine the effect of replacing perlite (PL) with pellets processed with poultry feather fiber as an inert material to prepare growing medium. The growth and flowering of Salvia splendens 'Vista Red' grown in individual growing medium $Biosangto^{(R)}$, peat moss (PM), PL, and two pellets (P45-1 and P45-2) were evaluated. Peat moss was mixed with PL, P45-1, or P45-2 at various ratios (1:0 to 1:3 or 3:1 by volume) to investigate the feasibility of replacing PL with pellets. Nutrient composition of the growing medium and leaf tissues was analyzed. The number of florets, inflorescence length, plant height, and fresh weight of plants grown in media containing P45-1 or P45-2 were reduced compared to those grown in individual growing medium PM or PL. As the mixing ratio of P45-1 or P45-2 to PM was higher, the growth of salvia, such as inflorescence length, plant height, number of leaves, and fresh weight was inhibited. Our results indicate that mixing three parts PM with one part of P45-1 (PM/P45-1/3:1) or P45-2 (PM/P45-2/3:1) accelerated flowering and increased the number of florets and leaves compared to other mixing ratios of PM and pellets media. The concentrations of phosphorus (P), calcium (Ca), boron (B), iron (Fe), and copper (Cu) in individual growing medium PL, P45-1, and P45-2 were significantly lower than those in PM. The concentration of N was the highest in leaves of plants grown in P45-1 or P45-2 amended media, and the concentrations of P, Ca, and zinc (Zn) in leaves were lower in individual growing medium P45-1 or P45-2 than in PM and PL. The pH of PM/P45-1/3:1 or PM/P45-2/3:1 media was maintained at optimal level (5.8-5.9) and the concentrations of macro- and micro-elements in the media and leaves were considered to be optimal levels. Therefore, mixing three parts PM with one part P45-1(PM:P45-1/3:1) or P45-2 (PM:P45-2/3:1) is recommended for improved growth and flowering in salvia. This suggests that P45-1 or P45-2 can replace PL as an inert material to prepare growing medium.

• 한국약용작물학회지
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• 제24권5호
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• pp.401-407
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• 2016

Background: Salvia has been widely cultivated for use in flavoring and folk medicines in many countries, including Korea and China. In this study, we investigated the anti-inflammatory activity and the underlying active compounds of Salvia extract and its fractions. Methods and Results: The anti-inflammatory activity was measured by assessing the inhibition of cysteinyl leukotriene production in rat basophilic leukemia (RBL)-2H3 mast cells. Salvia plebeia R. Br. was found to have the most potent inhibitory activity on leukotriene production than S. japonica and S. chanroenica had. Fifty percent ethanol extracts of S. plebeia R. Br. were successively partitioned with n-hexane, methylene chloride, ethyl acetate, 1-butanol and water. The ethyl acetate (EtOAc) fraction showed stronger anti-inflammatory activity than other solvent fractions did. The EtOAc fraction was subjected to silica gel column chromatography elution with a chloroform and methanol gradient system (100 : 1 ${\rightarrow}$ 1 : 1) yielding 10 fractions. Three kinds of fractions (chloroform:methanol = 20 : 1, 10 : 1 and 5 : 1) showed high inhibitory activity on leukotriene production. We confirmed the major compounds with anti-inflammatory activity from S. plebeia R. Br. Conclusions: In this study, the major components of S. plebeia that showed leukotriene production inhibitory activity were isolated using solvent extraction and silica gel column chromatography. Rosmarinic acid, hispidulin and luteolin were identified as the major compounds with anti-inflammatory effect.