DOI QR코드

DOI QR Code

Anti-Inflammatory Activity of Three Kinds of Salvia and Its Active Compounds

3종의 배암차즈기에 대한 염증억제 활성 비교 및 활성성분 구명

  • Received : 2016.08.19
  • Accepted : 2016.10.12
  • Published : 2016.10.30

Abstract

Background: Salvia has been widely cultivated for use in flavoring and folk medicines in many countries, including Korea and China. In this study, we investigated the anti-inflammatory activity and the underlying active compounds of Salvia extract and its fractions. Methods and Results: The anti-inflammatory activity was measured by assessing the inhibition of cysteinyl leukotriene production in rat basophilic leukemia (RBL)-2H3 mast cells. Salvia plebeia R. Br. was found to have the most potent inhibitory activity on leukotriene production than S. japonica and S. chanroenica had. Fifty percent ethanol extracts of S. plebeia R. Br. were successively partitioned with n-hexane, methylene chloride, ethyl acetate, 1-butanol and water. The ethyl acetate (EtOAc) fraction showed stronger anti-inflammatory activity than other solvent fractions did. The EtOAc fraction was subjected to silica gel column chromatography elution with a chloroform and methanol gradient system (100 : 1 ${\rightarrow}$ 1 : 1) yielding 10 fractions. Three kinds of fractions (chloroform:methanol = 20 : 1, 10 : 1 and 5 : 1) showed high inhibitory activity on leukotriene production. We confirmed the major compounds with anti-inflammatory activity from S. plebeia R. Br. Conclusions: In this study, the major components of S. plebeia that showed leukotriene production inhibitory activity were isolated using solvent extraction and silica gel column chromatography. Rosmarinic acid, hispidulin and luteolin were identified as the major compounds with anti-inflammatory effect.

Keywords

References

  1. Barnes PJ, Chung KF and Page CP. (1998). Inflammatory mediators of asthma: An update. Pharmacological Reviews. 50:515-596.
  2. Beasley R, Semprini A and Mitchell EA. (2015). Risk factors for asthma: Is prevention possible? The Lancet. 386:1075-1085. https://doi.org/10.1016/S0140-6736(15)00156-7
  3. Choi JK, Oh HM, Park JH, Choi JH, Sa KH, Kang YM, Park PH, Shin TY, Rho MC and Kim SH. (2015a). Salvia plebeia extract inhibits the inflammatory response in human rheumatoid synovial fibroblasts and a murine model of arthritis. Phytomedicine. 22:415-422. https://doi.org/10.1016/j.phymed.2015.01.007
  4. Choi SI, Kwak H, Kim JY, Choi JG and Lee JH. (2015b). Antiadipogenic effects of Salvia plebeia R. Br. extracts by extraction conditions in 3T3-L1 preadipocytes. Korean Journal of Medicinal Crop Science. 23:245-252. https://doi.org/10.7783/KJMCS.2015.23.3.245
  5. Drazen JM, Israel E and O'Byrne PM. (1999). Treatment of asthma with drugs modifying the leukotriene pathway. New England Journal of Medicine. 340:197-206. https://doi.org/10.1056/NEJM199901213400306
  6. Elias JA, Lee CG, Zheng T, Ma B, Homer RJ and Zhu Z. (2003). New insights into the pathogenesis of asthma. The Journal of Clinical Investigation. 111:291-297. https://doi.org/10.1172/JCI17748
  7. Goulet JL, Byrum RS, Key ML, Nguyen M, Wagoner VA and Koller BH. (2000). Genetic factors determine the contribution of leukotrienes to acute inflammatory responses. The Journal of Immunology. 164:4899-4907. https://doi.org/10.4049/jimmunol.164.9.4899
  8. Jung HJ, Song YS, Lim CJ and Park EH. (2009). Antiinflammatory, anti-angiogenic and anti-nociceptive activities of an ethanol extract of Salvia plebeia R. Brown. Journal of Ethnopharmacology. 126:355-360. https://doi.org/10.1016/j.jep.2009.08.031
  9. Liang Z, Xu Y, Wen X, Nie H, Hu T, Yang X, Chu X, Yang J, Deng X and He J. (2016). Rosmarinic acid attenuates airway inflammation and hyperresponsiveness in a murine model of asthma. Molecules. 21:769-774. https://doi.org/10.3390/molecules21060769
  10. Lim JA, Yun BW and Baek SH. (2007). Antioxidative activity and nitrite scavenging ability of methanol extract from Salvia plebeia R. Br. Korean Journal of Medicinal Crop Science. 15:183-188.
  11. Maggi E. (1998). The TH1/TH2 paradigm in allergy. Immunotechnology. 3:233-244. https://doi.org/10.1016/S1380-2933(97)10005-7
  12. Minoguchi K and Adachi M. (1999). Pathophysiology of asthma. In Cherniack NS, Altose MD, Homma I(ed.)., Rehabilitation of the patient with respiratory disease. McGraw-Hill. New York. NY, USA. p.97-104.
  13. Pawankar R. (2001). Mast cells as orchestrators of the allergic reaction: The IgE-IgE receptor mast cell network. Current Opinion in Allergy and Clinical Immunology. 1:3-6.
  14. Ren DB, Qin YH, Yun YH, Lu HM, Chen XQ and Liang YZ. (2014). Separation of nine compounds from Salvia plebeia R. Br. using two-step high-speed counter-current chromatography with different elution modes. Journal of Separation Science. 37: 2118-2125. https://doi.org/10.1002/jssc.201400293
  15. Shimizu H, Obase Y, Katoh S, Mouri K, Kobashi Y and Oka M. (2013). Critical role of interleukin-5 in the development of a mite antigen-induced chronic bronchial asthma model. Inflammation Research. 62:911-917. https://doi.org/10.1007/s00011-013-0651-y
  16. Wills-Karp M, Luyimbazi J, Xu X, Schofield B, Neben TY, Karp CL and Donaldson DD. (1998). Interleukin-13: Central mediator of allergic asthma. Science. 282:2258-2261. https://doi.org/10.1126/science.282.5397.2258
  17. Wynn TA. (2015). Type 2 cytokines: Mechanisms and therapeutic strategies. Nature Reviews Immunology. 15:271-282. https://doi.org/10.1038/nri3831

Cited by

  1. Effects of Salvia plebeia R. Br. on Antioxidative Enzyme Activities and Oxidative Damage in Rats Fed High-Fat and High-Cholesterol Diets vol.33, pp.4, 2018, https://doi.org/10.13103/JFHS.2018.33.4.316