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Resistance to ACCase Inhibitor Cyhalofop-butyl in Echinochloa oryzicola Collected in Gyeongsangnam-do Province of Korea (ACCase 저해 제초제 cyhalofop-butyl에 대한 경남지방 수집종 피의 저항성)

  • Won, Jong Chan;Won, Ok Jae;Ha, Jun;Im, Il-Bin;Kang, Kwang Sik;Pyon, Jong Yeong;Park, Kee Woong;Lee, Jeung Joo
    • Weed & Turfgrass Science
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    • v.7 no.2
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    • pp.166-169
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    • 2018
  • Repeated use of ACCase inhibiting herbicides for a long time has resulted in increases of resistant Echinochloa oryzicola populations in paddy fields in middle west area of Korea. This study aims to investigate current status of herbicide resistant E. oryzicola in Gyeongsangnam-do, in which there is less information about herbicide resistance. For resistance frequency and dose-response study, seeds from 100 individual plants of E. oryzicola in Gyeongsangnam-do were collected and tested with cyhalofop-butyl. Seven percent of plants from Gyeongsangnam-do was resistant at a recommended rate of cyhalofop-butyl. $GR_{50}$ values (herbicide rates required to reduce plant growth 50%) for one representative resistant populations and five susceptible populations were $738g\;a.i.\;ha^{-1}$ and 66-234 (average 147)$g\;a.i.\;ha^{-1}$, respectively, indicating average 5 times difference in resistance. Although lower rate of frequency of herbicide resistance in Gyeongsangnam-do than in Jeollabuk-do, increases of herbicide resistance are expected in this area because of increases of direct seeded rice fields and increases of dependence on a specific herbicide. Therefore, it is necessary to monitor herbicide resistance regularly and conduct integrated herbicide resistance management in this area.

Predictive mathematical model for the growth kinetics of Listeria monocytogenes on smoked salmon (온도와 시간을 주요 변수로한 훈제연어에서의 Listeria monocytogenes 성장예측모델)

  • Cho, Joon-Il;Lee, Soon-Ho;Lim, Ji-Su;Kwak, Hyo-Sun;Hwang, In-Gyun
    • Journal of Food Hygiene and Safety
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    • v.26 no.2
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    • pp.120-124
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    • 2011
  • Predictive mathematical models were developed for predicting the kinetics of growth of Listeria monocytogenes in smoked salmon, which is the popular ready-to-eat foods in the world, as a function of temperature (4, 10, 20 and $30^{\circ}C$). At these storage temperature, the primary growth curve fit well ($r^2$=0.989~0.996) to a Gompertz equation to obtain specific growth rate (SGR) and lag time (LT). The Polynomial model for natural logarithm transformation of the SGR and LT as a function of temperature was obtained by nonlinear regression (Prism, version 4.0, GraphPad Software). Results indicate L. monocytogenes growth was affected by temperature mainly, and SGR model equation is $365.3-31.94^*Temperature+0.6661^*Temperature^{\wedge^2}$ and LT model equation is $0.1162-0.01674^*Temperature+0.0009303^*Temperature{\wedge^2}$. As storage temperature decreased $30^{\circ}C$ to $4^{\circ}C$, SGR decreased and LT increased respectively. Polynomial model was identified as appropriate secondary model for SGR and LT on the basis of most statistical indices such as bias factor (1.01 by SGR, 1.55 by LT) and accuracy factor (1.03 by SGR, 1.58 by LT).

Effect of Long Period Usage of Polyolefin Film on Growth and Fruit Quality in Korea Melon(Cucumis melo L. var makuwa Makino) (폴리올레핀계 필름 장기사용이 참외의 생육 및 품질에 미치는 영향)

  • Shin, Yong-Seub;Yeon, Il-Kweon;Lee, Ji-Eun;Cheong, Jong-Do;Choi, Seong-Yong;Chung, Doo-Seok
    • Journal of Bio-Environment Control
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    • v.18 no.1
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    • pp.9-14
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    • 2009
  • This experiment was conducted to investigate the difference among 3-years-used Polyolefin films which were J-1, J-2 and J-3 having differences in film thickness, infrared absorption and ultraviolet penetration from Jan. 16,2006. And 1-year-used Polyethylene film K-1 used from Jan. 16,2008, for covering film of greenhouse for korea melon cultivation. J-2, J-3 and J-1 films were better for keeping heat in order, and J-2 film was the best in plant growth at early stage. The first blooming and harvesting days in J-2 film were earlier 10 days than those in K-1 film. Chromaticity and soluble solid of harvested fruit in J-3, J-1 and J-2 films were higher than those in K-1 film. Marketable yields in J-2, J-1 and J-3 films were higher in order.

Effect of Long Time Usage of Soft Film on the Growth and Yield in Oriental Melon (Cucumis melo L. var makuwa Makino) (연질필름의 장기사용이 참외의 생육 및 수량에 미치는 영향)

  • Shin, Yong-Seub;Yeon, Il-Kweon;Lee, Ji-Eun;Do, Han-Woo;Cheung, Jong-Do;Park, Jong-Wook;Choi, Seong-Yong;Chung, Doo-Seok
    • Journal of Bio-Environment Control
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    • v.19 no.3
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    • pp.135-139
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    • 2010
  • This experiment was conducted to investigate the difference among 3-years-used polyolefin films which were J-l, J-2 and J-2 having differences in film thickness, infrared absorption and ultraviolet penetration from Jan. 16, 2006. And 1-year-used polyethylene film K-1 used from Jan. 16, 2009, for covering film of greenhouse for oriental melon cultivation. J-2, J-3 and J-1 films were better for keeping heat in order, and J-2 film was the best in plant growth at early stage. The first blooming and harvesting days in J-2 film were earlier 15 days than those in K-1 film. Chromaticity and soluble solid of harvested fruit in J-2, J-3 and J-1 films were higher than whose in K-1 film. Marketable yields in J-2, J-3 and J-1 films were higher in order.

Study on Hwa-acupuncture Theory (오행화침법(五行和鍼法)에 대한 연구 - 부방(腑方)중심으로)

  • Sim, Sung-Heum;Kam, Cheol-Woo;Park, Dong-Il;Byun, Mi-Kwon;Kim, Sang-Heon;Baek, Sang-In
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.5
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    • pp.1119-1124
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    • 2008
  • The theory of Hwa Acupuncture Therapy (HAT), called Hwa Chim Therapy, is a part of the Five Elements Theory unique to Korea. H99AT, created and developed by Jaehoon Song, integrates the victor-vanquished as well as the son-mother relationship of the Five Elements of breakdown and restoration of balance between yin and yang. And also, it provides resources and data on The seventy fifth Difficulty Nan(75難), The sixty ninth Difficulty Nan(69難) of Classic on Difficulty - Nan Jin 75, 69. HAT establishes objectiveness and accuracy of diagnosis based upon the traditional method and procedure of pulse taking. In HAT, a person's state of illness is diagnosed by applying the comparative examination of the palpitation of the pulse. It is the fact that the pulse varies according to the state, and that HAT treatment has proven the positive results by using the victor-vanquished relationship of Classic on The Nan Jin 75. On the basis of this, it is necessary to add the sixty ninth Difficulty(69難), to research the theory of the generation of the Five Element. Despite a the concise and simple theory, Hwa Chim is very effective in treating the wide range of illness, and thus it has gained an increasing attention of many scholars and practitioners in the field of traditional Korean oriental medicine. However, it is the first theoretical attempt to the clinical research and scientific methodology of Ohaeng(Five) Hwa Chim, and more active Hwa Acupuncture R&D is being conducted nationwide.

Proposing a Technique for Regional Flood Frequency Analysis: Bayesian-GLS Regression (국내 지역 홍수빈도해석을 위한 기법 제안: Bayesian-GLS 회귀)

  • Jeong, Dae-Il;Stedinger, Jery R.;Kim, Young-Oh;Sung, Jang-Hyun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2007.05a
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    • pp.241-245
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    • 2007
  • 국내 홍수빈도 분포의 매개변수 추정에서 지점추정(at-site estimate) 방법은 유량 자료의 부족으로 발생하는 표본오차(sampling error)가 크기 때문에 충분한 유량 자료를 보유한 지점에 한하여 제한적으로 사용되고 있다. 대안으로 동질성을 가진 유역의 유량 자료를 모아 지역 매개변수를 추정하는 지수홍수법(Index Flood Method)이 제안되기도 하였으나, 이질성이 큰 우리나라의 유역특성 때문에 적용이 쉽지 않다. Stedinger와 Tasker가 1986년 제안한 GLS(Generalized Least Square) 기법은 유역을 동질지역으로 구분할 필요가 없으며 지점들간의 상관관계와 이분산성을 고려할 수 있어, 국내 홍수빈도 해석을 위해서 꼭 도입해야할 기법으로 생각된다. 본 연구에서는 기존의 GLS 기법의 단점을 보완한 Bayesian-GLS 기법을 이용하여, 국내 대유역에 골고루 위치하며 댐의 영향을 받지 않는 31개 지점의 연최대 일유량 시계열의 L-변동계수(L-moment coefficient variation)와 L-왜도계수(L-moment coefficient skewness)를 추정할 수 있는 회귀모형을 제안하였다. 위 회귀모형을 구성하기 위한 유역특성으로는 유역면적, 유역경사, 유역평균강우 등을 사용하였다. Bayesian-GLS (B-GLS) 적용 결과를 OLS(Ordinary Least Square) 및 Bayesian-GLS 기법에서 지점간의 상관관계를 고려하지 않는 Bayesian-WLS(Weighted Least Square)와 비교 평가하여 그 우수성을 입증하였다. 따라서 본 연구에서 제안된 B-GLS에 의한 지역회귀모형은 국내의 미계측유역이나 또는 관측 길이가 짧은 계측유역의 홍수빈도분석을 위해 매우 유용할 것으로 기대된다.년 홍수 피해가 발생하고 있지만, 다른 한편 인구밀도가 높고 1인당 가용 수자원이 상대적으로 적기 때문에 국지적 물 부족 문제를 경험하고 있다. 최근 국제적으로도 농업용수의 물 낭비 최소화와 절약 노력 및 타 분야 물 수요 증대에 대한 대응 능력 제고가 매우 중요한 과제로 부각되고 있다. 2006년 3월 멕시코에서 개최된 제4차 세계 물 포럼에서 국제 강 네트워크는 "세계 물 위기의 주범은 농경지", "농민들은 모든 물 위기 논의에서 핵심"이라고 주장하고, 전 프랑스 총리 미셀 로카르는 "...관개시설에 큰 문제점이 있고 덜 조방적 농업을 하도록 농민들을 설득해야 한다. 이는 전체 농경법을 바꾸는 문제..."(segye.com, 2006. 3. 19)라고 주장하는 등 세계 물 문제 해결을 위해서는 농업용수의 효율적 이용 관리가 중요함을 강조하였다. 본 연구는 이러한 국내외 여건 및 정책 환경 변화에 적극적으로 대처하고 물 분쟁에 따른 갈등해소 전략 수립과 효율적인 물 배분 및 이용을 위한 기초연구로서 농업용수 수리권과 관련된 법 및 제도를 분석하였다.. 삼요소의 시용 시험결과 그 적량은 10a당 질소 10kg, 인산 5kg, 및 가리 6kg 정도였으며 질소는 8kg 이상의 경우에는 분시할수록 비효가 높았으며 특히 벼의 후기 중점시비에 의하여 1수영화수와 결실율의 증대가 크게 이루어졌다. 3. 파종기와 파종량에 관한 시험결과는 공시품종선단의 파종적기는 4월 25일부터 5월 10일경까지 인데 이 기간중 일찍 파종하는 경우에 파종적량은 10a당 약 8${\ell}$이고 늦은 경우에는 12${\ell}$ 정도였다. 여기서 늦게 파종한 경우 감수의 가장 큰 원인은 1수영화수가 적어지기 때문이었다. 4. 건답직파에 대한 담수상태로 관수를 시작하는 적기는 파종후

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Optimization of Medium for Astaxanthin Production by Paracoccus sp. Using Response Surface Methodology (반응표면분석 법을 이용한 Paracoccus sp.의 Astaxanthin 생산배지 최적화)

  • Choi, Jong-Il;Lee, Hee-Sub;Choi, Seon-Kang;Kim, Jae-Hun;Kim, Jin-Kyu;Misawa, Norihiko;Byun, Myung-Woo;Lee, Ju-Woon
    • KSBB Journal
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    • v.24 no.3
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    • pp.321-326
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    • 2009
  • This study was to optimize the medium components for astaxanthin production in Paracoccus sp. through surface response methodology. A screening test was first conducted on 5 medium components using a Plackett-Burman design, from which $MgSO_4$ and yeast extract were identified as the significant factors affecting astaxanthin production. These significant factors were optimized by central composite design of experiments and response surface methodology, as 2.83 g/L $MgSO_4$ and 7.02 g/L yeast extract, respectively. The expected astaxanthin concentration with these optimized medium compositions were 0.925 mg/L. In flask culture, the experimentally obtained concentration of astaxantin was 1.021 mg/L, where it had been 0.4 mg/L before optimization.

Alkenylation of o-xylene with 1,3-Butadiene Over Base Catalysts (염기성 촉매를 이용한 o-xylene과 1,3-Butadiene의 알케닐화 반응)

  • Lee, Jong Seok;Lee, Soo Chool;Kil, Min Ho;Choi, Il Seok;Lee, Jae Sung;Kim, Jae Chang
    • Korean Chemical Engineering Research
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    • v.40 no.6
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    • pp.669-675
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    • 2002
  • The alkenylation of o-xylene with 1,3-butadiene to make OTP(ortho-tolyl Pentene) was carried out over liquid phase NaK alloy, Na metal and the metallic sodium dispersed on the specific support such as NaX and $Al_2O_3$. Liquid phase NaK alloy showed the improved conversion and selectivity when they were pretreated by ultrasound to increase the dispersion. For the case of metallic sodium, the induction period for the formation of homogeneous metal sodium solution with high dispersion was needed before the reaction. In the case of metallic sodium dispersed on support, more than 80 % conversion could be obtained without induction period regardless of supports used. But 85 % of the metallic sodium was resolved into the reaction mixture after reaction for 7 hours. The amount of byproducts, oligomers, produced from OTP and 1,3-butadiene increased with the amount of 1,3-butadiene introduced and the selectivity to OTP was in inversely proportional to the conversion.

Characterization of Rice Mutants with Enhanced Susceptibility to Rice Blast

  • Kim, Hye-Kyung;Lee, Sang-Kyu;Cho, Jung-Il;Lee, Sichul;An, Gynheung;Jwa, Nam-Soo;Kim, Byung-Ryun;Cho, Young-Chan;Han, Seong-Sook;Bhoo, Seong-Hee;Lee, Youn-Hyung;Hong, Yeon-Kyu;Yi, Gihwan;Park, Dae-Sup;Hahn, Tae-Ryong;Jeon, Jong-Seong
    • Molecules and Cells
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    • v.20 no.3
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    • pp.385-391
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    • 2005
  • As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance, we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 (91-033) from a T-DNA insertion library of the japonica rice cultivar, Hwayeong. Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning strategy to isolate the gene(s) responsible for the enhanced susceptibility of the Hwayeong mutants. A genetic mapping population was produced by crossing the resistant wild type Hwayeong with the susceptible cultivar, Nagdong. Chi-square analysis of the $F_2$ segregating population indicated that resistance in Hwayeong was controlled by a single major gene that we tentatively named Pi-hy. Randomly selected susceptible plants in the $F_2$ population were used to build an initial map of Pi-hy. The SSLP marker RM2265 on chromosome 2 was closely linked to resistance. High resolution mapping using 105 $F_2$ plants revealed that the resistance gene was tightly linked, or identical, to Pib, a resistance gene with a nucleotide binding sequence and leucine-rich repeats (NB-LRR) previously isolated. Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene, demonstrating that the Pi-hy gene is Pib. The Pib mutations in 1D-22-10-13, 1D-54-16-8, and 1C-143-16-1 were, respectively, a missense mutation in the conserved NB domain 3, a nonsense mutation in the 5th LRR, and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus. These findings provide evidence that NB domain 3 and the C terminus are required for full activity of the plant R gene. They also suggest that alterations of the resistance gene can cause major differences in pathogen specificity by affecting interactions with an avirulence factor.

Feeding Behavior in the Plant Tissues with Green Peach Aphid (Myzus persicae, Aphididae; Homoptera) Using EPG Technique (EPG를 이용한 복숭아혹진딧물 (Myzus persicae, Aphididae, Homoptera)의 기주 식물체별 조직내 섭식행동)

  • Seo, M.J.;Jang, J.K.;Kang, E.J.;Kang, M.K.;Kim, N.S.;Yu, Y.M.;Youn, Y.N.
    • Korean journal of applied entomology
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    • v.44 no.4 s.141
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    • pp.271-276
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    • 2005
  • To investigate feeding behaviour of the greenpeach aphid (Myzus persicae) on several plants, DC electrical penetration graph (EPG) technique was used. We chose 5 plants including pepper, melon, cabbage, radish, and eggplant which were known as major host Plants of this species. This study was focused whether feeding patterns of the aphid were different and which plants would be the most preferable among 5 host plants. The time from initial proboscis contact with a each leaf until the first electrical contact, as a measure of the time taken for the stylet penetration, the time from electrical contact to the first potential drop as a time consumed until intracellular sampling, the number of potential drops per an hour during periods of regular intercellular pathway probing, and the time from electrical contact to tile first phloem specific pattern indicating the time taken to reach and attempt to feed upon the phloem were analysed. As a result, except the number of potential drop, there was no significant differency of feeding patterns among 5 plants. However, the feeding patterns related on host acceptability were observed more frequently from Pepper, radish, and e99r1an1 than melon and cabbage.