• Korean Journal of Microbiology
• /
• v.24 no.3
• /
• pp.211-220
• /
• 1986

Nitrogen fixation (nif) genes of Rhizobium leguminosarum were transferred to nif Klebsiella pneumoniae and E. coli by conjugation after partial heat induction of $RP_4$ :: Mu cts in Rhizobium $R^+$ transconjugant, and the hybrid plasmids in the transconjugant strains were isolated and characterized. In order to transfer the nif genes from Rhizobium, the hybrid plasmid $RP_4$ :: Mu cts was transferred by conjugation from E. coil to the symbiotic nitrogen fixer, R. leguminosarum. After stabillity test, the $RP_4$ :: Mu cts in Rhixobium $R^+$ transconjugant was subjected to partial heat induction by culturing it statically at $38^{\circ}C$ for 16 hours, and then conjugated with the nif defective mutant strains of K. pneumoniae or nif mutant strains of E. coli having whole nif gene plasmid. Recombinant strains of K. pneumoniae, which could grow in a N-free medium and exhibit the nitrogenase activity were selected. However, in the case of E. coli, they could grow well in a NA medium containing antibiotices, but hardly frow in a N-free medium. The hybrid plasmids in these transconjugal strains were isolated by gel electrophoresis and compared their molecular sizes.

• The Journal of Fisheries Business Administration
• /
• v.40 no.3
• /
• pp.169-187
• /
• 2009

This study analyzes the long and short term effect of exchange rate on the import of Korea's fisheries focussed on main fisheries imported from China. The estimation models consist of the following contents. The first model consists of one dependent variable-import quantity of fisheries imported from China(${IMQ_t}^{CHO}$) and three independent variables-${RP_t}^{CHO}$, $EXC_t$ and $GDP_t$. The second one-one dependent variable-import quantity of fisheries imported from China(${JMQ_t}^{NAG})$ and three independent variables-${RP_t}^{NAG}$, $EX_t$ and $GDP_t$. the third one-one dependent variable-import quantity of fisheries imported from China(${IMQ_t}^{AH}$) and three independent variables-${RP_t}^{AH}$, $EX_t$ and $GDP_t$. the forth one-one dependent variable-import quantity of fisheries imported from China(${IMQ_t}^{KO}$) and three independent variables-${RP_t}^{KO)$, $EX_t$ and $GDP_t$. the last one is made up of one dependent variable-import quantity of fisheries imported from China(${IMQ_t}^{GAL}$) and three independent variables-, ${RP_t}^{GAL}$, $EX_t$ and $GDP_t$. and. The estimation results show that exchange rate of the independent variables are statistically significant in only the first model. The figure is elastic. Especially, the effect of exchange rate in first model is grater than that of the. However, the effect of exchange rate, one of independent variables in the ECM, is not statistically significant.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.24 no.3 s.174
• /
• pp.769-776
• /
• 2000

RP(Rapid Prototyping) technology is gaining its popularity in building a prototype in all industries. SLS(Slective Laser Sintering) is one of RP technologies, which is focused on tooling processes as well as three dimension solid model. There are several factors, the length and the cross-sectional area of a part, that have an effect on build setup in SLS process. In this paper, the computation on geometrical relationship is used to slice STL file and to estimate these factors. Based on these values, the build setup parameters such as the heating temperature, the laser power, and the powder cartridge feed rate are determined by neural network approaches. The test results show that the computation time is saved and the neural network approach is able to apply to get the optimal parameters of build process within an acceptable error rate.

• Journal of Internet Computing and Services
• /
• v.3 no.5
• /
• pp.87-94
• /
• 2002

This paper proposes a group key management protocol for a secure of all the multcast user in PIM-SM multicast group communication. Each subgroup manager gives a secure key to it's own transmitter and the transmitter compress the data with it's own secure key from the subgroup manager, Before the transmitter send the data to receiver, the transmitter prepares to encrypt a user's service by sending a encryption key to the receiver though the secure channel. after checking the user's validity through the secure channel, As the transmitter sending a data after then, the architecture is designed that the receiver will decode the received data with the transmitter's group key, Therefore, transmission time is shortened because there is no need to data translation by the group key on data sending and the data transmition is possible without new key distribution at path change to shortest path of the router characteristic.

• Journal of the Korean Society of Floral Art and Design
• /
• no.42
• /
• pp.117-132
• /
• 2020

The study aimed at trying to contribute to domestic floral designers by analyzing colors' images of 60 floral designs of British floral designers, Shane Connolly and Paula Pryke, as the basis for color scheme of floral space design. The result of color analysis, Shane Connolly uses colors in the frequency of color order of the GY>Y>RP>YR>P>P>PB and Paula Pryke uses colors in the frequency of color order of the GY>RP>P>YR,Y>G>PB. The result of coordinate analysis of hue, value, chrome, Shane Connolly's works showed high frequency in the YtoGY color, the middle and high of value and the middle and high of chrome. Paula Pryke's works showed high frequency in the GY color and PtoRP color, the wide areas of the 3.8 ~9 stages of value, and the middle and high of chrome. The result of adjective image scale, Shane Connolly's works were found to be in between soft and static adjective zone, and Paula Pryke's works were distributed in between soft and active zone.

• Journal of the Korean Society for Precision Engineering
• /
• v.21 no.7
• /
• pp.30-36
• /
• 2004

Recently, the lead-time of a product is to be shortened in order to satisfy consumer's demand. It is thus important to reduce the manufacturing time and the cost of 3D-shaped microstructures. Micro-Electro-Mechanical Systems (MEMS) and devices are usually fabricated by lithography-based methods. Above method is not flexible for the rapid manufacture of 3D-shaped microstructures because it depends on work's experiences and requires excessive cost and time for making many masks. In this paper, the effective laser micrornachining is developed to fabricate UV sensitive polymer microstructures using laser ablation. The proposed process, named by laser microRP, is a very useful method on rapid manufacturing for 3D-shaped microstructures.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.16
• /
• pp.6871-6879
• /
• 2014

Background: The purpose of this study was to evaluate the potential association of five (p.P47S, p.R72P, PIN3 Ins16bp, p.R213R and r.13494g>a) polymorphisms of TP53 with the risk of developing breast cancer in North Indian Punjabi population. Methods: We screened DNA samples of 200 sporadic breast cancer patients (197 females and 3 males) and 200 unrelated healthy, gender and age matched individuals for the polymorphisms. Results: For the p.P47S polymorphism, we observed the PP genotype in 99.5% of the patients and PS genotype in only 1 patient. All the controls had the wild type PP genotype. The frequency of RR, RP and PP genotype of p.R72P was 23.5% vs 33.5%, 51.5% vs 45.5% and 25% vs 21% in patients and controls respectively. Heterozygous (RP) genotype was increased in breast cancer patients as compared to controls (51.5 vs 45.5%) and showed 1.61 fold significantly increased risk for breast cancer (OR=1.61, 95% CI, 1.01-2.58, p=0.04). In breast cancer patients the frequencies of A1A1, A1A2 and A2A2 genotypes of PIN3 Ins16bp polymorphism were 67%, 26% and 7% respectively whereas in controls the genotype frequencies were 68.5%, 27.5% and 4% respectively, with no significant difference. For p.R213R (c.639A>G), all individuals had homozygous wild type genotype. The frequencies of GG, GA and AA genotypes of TP53 r.13494g>a polymorphism were 62 vs 67.5%, 33 vs 28% and 5 vs 4.5% in patients and controls respectively, again without significant difference. We observed that RP-A1A1 genotype combination of p.R72P and PIN3 Ins16bp and RP-GG combination of p.R72P and r.13494g>a polymorphism showed significant risk of breast cancer (OR=1.65, 95%CI: 0.98-2.78, p=0.05; OR=1.72, 95%CI: 1.01-2.92, p=0.04). Conclusion: The results of present study indicated that among the five TP53 polymorphisms investigated, the p.R72P polymorphism, and the RP-A1A1 and RP-GG genotype combination contribute to breast cancer susceptibility in North Indians.

• Korean Journal of Agricultural Science
• /
• v.48 no.4
• /
• pp.669-679
• /
• 2021

This study was conducted to evaluate the effects of rumen-protected amino acid (RPAA) prototypes, which were chemically synthesized, on in vitro rumen fermentation and protection rate outcomes. Several RPAA prototypes were incubated with timothy hay and concentrate. Treatments consisted of 1) control (CON; no RPAA prototype supplement), and prototypes of 2) 0.5% RP-methionine (RPMet), 3) 0.5% RP-tryptophan (RPTrp), 4) 0.5% RP-valine (RPVal), 5) 0.5% RP-phenylalanine (RPPhe), 6) 0.5% RP-leucine (RPLeu), 7) 0.5% RP-histidine (RPHis), 8) 20% RPMet, and 9) 20% RPTrp (w·w^-1 feed). The inoculum (50 mL) prepared with rumen fluid and McDougall's buffer (1 : 4) was dispensed in individual serum bottles and was anaerobically incubated for 0, 6, and 24 h at 39℃ in triplicate. The dry matter degradability did not differ among the groups, except for the 20% RPMet and the 20% RPTrp treatments at 6 and 24 h. The total volatile fatty acid concentration in the 20% RPMet was higher (p < 0.05) than the rest of the groups at 6 h, and 20% RPMet showed the highest molar proportion of acetate, whereas the lowest proportion of propionate was found at 6 h (p < 0.05). The protection rate of the RPAA prototypes ranged from 29.85 to 109.21%. at 24 h. In conclusion, the chemically synthesized RPAA prototypes studied here had no detrimental effects on rumen fermentation parameters. Further studies using animal models are needed for more accurate evaluations of the effectiveness of RPAA.

• Transactions of the Korean Society of Machine Tool Engineers
• /
• v.11 no.2
• /
• pp.30-35
• /
• 2002

Nowadays, as the development period of new products becomes shorter and consumer's requirement is more various, the importance of Rapid Prototyping Technology has been rapidly increased. Rapid Prototyping makes prototypes or frictional parts directly using the 3D CAD data. But RP machines can make prototypes in limit size. For making large size prototype, we slice solid which is made of STL file, and then glue sliced solid. And if contact area of part is small, union solid will be easily destroyed for going down of adhesion. So we need to expand contact area, 1 suggest making a section into stair shape. This paper is concerned with slicing solid on STL file and improving on adhesion.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.3
• /
• pp.587-593
• /
• 2010

Three hundred and seventy-four rhizobacteria were isolated from the rhizosphere soil (RS) or rhizoplane (RP) of Echinochloa crus-galli, Carex leiorhyncha, Commelina communis, Persicaria lapathifolia, Carex kobomugi, and Equisetum arvense, grown in contaminated soil with petroleum and heavy metals. The isolates were screened for plant growth-promoting trait (PGPT), including indole acetic acid (IAA) productivity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and siderophore(s) synthesis ability. IAA production was detected in 86 isolates (23.0%), ACC deaminase activity in 168 isolates (44.9%), and siderophore(s) synthesis in 213 isolates (57.0%). Among the rhizobacteria showing PGPT, 162 isolates had multiple traits showing more than two types of PGPT. The PGPT-possesing rhizobacteria were more abundant in the RP (82%) samples than the RS (75%). There was a negative correlation (-0.656, p<0.05) between the IAA producers and the ACC deaminase producers. Clustering analysis by principal component analysis showed that RP was the most important factor influencing the ecological distribution and physiological characterization of PGPT-possesing rhizobacteria.