• Journal of Life Science
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• v.20 no.8
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• pp.1254-1260
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• 2010

Microorganisms near the plant rhizosphere usually inhabit the surface or the inside of the plant roots and have a direct effect on plant growth by secreting plant growth promoters or antagonistic materials which protect the root zone system from various pathogens. This study was carried out to identify and isolate the antagonistic materials after isolation of microorganisms showing high antagonistic activities, in hopes of contributing to the development of sustainable agriculture and the preservation of agricultural environments. A number of antagonistic bacteria were isolated from paddy soil. Among isolates, RRj 228 showed plant growth promotion and antagonistic activity. RRj 228 was identified as Pseudomonas sp. according to the results of physiological properties and genetic methods. On the basis of the results of anti-fungal spectrum against several pathogens by RRj 228, the antagonistic effect of the isolate against Botrytis cinerea, Pythium ultimum, Phytopthola capsici, and Rhizoctonia solani, especially against red-pepper anthracnose caused by Colletotrichum acutatum, was remarkable. The experiment evaluating the biological control effect by RRj 228 revealed that the $ED_{50}$ value by the RRj 228 culture against C. acutatum, R. solani and P. ultimum were 0.14 mg/ml, 0.16 mg/ml and 0.29 mg/ml, respectively. An antagonistic substance was isolated and purified by several chromatographies from the RRj 228 culture. The $^1H$ and $^{13}C$ assignment of the antagonistic substance was achieved from two-dimensional $^1H-^1H$ COSY, HMQC, and HMBC. Finally, the antagonistic substance was identified as Phenazine-1-carboxylic acid ($C_{13}H_8N_2O_2$, M.W.=224).

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.4
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• pp.750-756
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• 2008

Supernumerary teeth are frequently found in the anterior portion of the maxilla and develop as a result of abnormal proliferation of the dental lamina during tooth germ formation, caused by genetic or environmental factors. They may result in various complications, such as eruption interference, displacement, rotation of adjacent teeth, diastema, eruption into the nasal cavity, and development of dentigerous cyst. The optimal time for surgical extraction of supernumerary teeth has been a controversial issue. Someone prefer early surgical extraction because supernumerary teeth can cause eruption interference and displacement of adjacent teeth, eventually altering occlusion. Others prefer to delay surgical extraction until $8{\sim}10$ years of age in consideration of root maturation of the adjacent teeth and also patient's behavior. When surgical extraction of supernumerary teeth is postponed, there is possibility that impacted supernumerary teeth in the inverted or horizontal position move toward the nasal cavity, hard palate, or premolar area. When such intraosseous tooth migration is combined with the vertical growth of the maxilla, surgical approach becomes even harder. Therefore, possibility of intraosseous tooth migration should be considered as an important factor when deciding appropriate time for surgical extraction. We are presenting cases of mesiodens which showed intraosseous migration during $6{\sim}7$ years of follow-up period since the first diagnosis had been made at the $2{\sim}3$ years of age.

• Journal of Korean Society of Forest Science
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• v.103 no.4
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• pp.593-598
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• 2014

In this study, we developed the carbon emission factors for 4 major species of warm-temperate region in Korea, and tried to provide their carbon emissions and removals estimates using these carbon emission factors. We selected Castanopsis cuspidata, Camellia japonica, Quercus acuta and Quercus glauca as target species and derived their carbon emission factors. The basic wood density that serve as one of the carbon emission factors were 0.583 for Castanopsis cuspidata, 0.657 for Camellia japonica, 0.833 for Quercus acuta and 0.763 for Quercus glauca and their uncertainties ranged from 5.3 to 17.9%. Biomass expansion factors were calculated as well: 1.386 for Castanopsis cuspidata, 2.621 for Camellia japonica, 1.701 for Quercus acuta and 2.123 for Quercus glauca and associated uncertainties varied from 14.7 to 30.5%. Lastly root-shoot ratios for each species were also determined: 0.454 for Castanopsis cuspidata, 0.356 for Camellia japonica, 0.191 for Quercus acuta and 0.299 for Quercus glauca with the uncertainties lying within a range from 19.8 to 35.7%. These three carbon emission factors including basic wood density had the uncertainties of less than 40% recommended by FAO. Therefore the application of country-specific emission factors seemed to provide quite accurate estimates of carbon emissions and removals. The estimation of the carbon stored in the 4 species were also conducted which amounted to $186.10tCO_2/ha$ for Castanopsis cuspidata, $280.63tCO_2/ha$ for Camellia japonica, $344.04tCO_2/ha$ for Quercus acuta and $278.91tCO_2/ha$ for Quercus glauca and their annual carbon removals were $6.65tCO_2/ha/yr$, $6.25tCO_2/ha/yr$, $11.70tCO_2/ha/yr$ and $12.29tCO_2/ha/yr$, respectively. This systematic assessment of forest resources can be a reliable source of information for managing evergreen broadleaved forest in warm temperate regions and thus serve as useful data for effective decision-making to address vegetation zone shifts due to climate change.

• Korean Journal of Agricultural Science
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• v.23 no.1
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• pp.108-119
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• 1996

Several parameters of hydraulic architecture relating to hydraulic conductance in xylem vessels were investigated in the current-year shoots of six species of deciduous oak trees. The above parameters were also investigated in the sprouts of Quercus mongolica and Q. variabilis, as well as in the seedlings of Q. mongolica and Q. acutissima. The values of specific conductivity, leaf specific conductivity and Hagen-Poiseuille's relative hydraulic conductivity relating to vessel diameter of Q. dentata were the highest in all of the species studied. The above values of most of the species studied were higher in May-June than in September-October because of increasing the vessel embolism by cavitation and so on through the growing season. The estimated values of relative hydraulic conductivity of vessel by Hagen-Poiseuille's empirical equation and the real values of hydraulic conductivity presented positive relationships in most of the species studied. Huber value and leaf specific conductity using leaf area or leaf weight generally exhibited similar patterns each other even if having some exceptions. The hydraulic conductances of sprouting shoots were much better than those of normal growing shoots in Q. rnongolica and Q. variabilis. The specific conductivity and leaf specific conductivity were rapidly decreased by the vessel embolism through cavitating just after cutting the shoots in Q. mongolica and Q. acutissima seedlings. Diurnal changes of the conductivities in the seedlings of Q. mongolica and Q. acutissima presented the possibility of their self-controlling of conductance by active moisture absorption under mild water stress. Specific conductivity and leaf specific conductivity, and so on of Q. acutissima seedlings subjected to periodical moisture stress or not have decreased through the growing season, but the influences of moisture stress to the conductance were not proved definitely because of influencing similarly and simultaneously to the development of xylem and leaf having inverse relation in the influences. The values of conductivities were higher generally in middle or upper parts of stems than root collar in the seedlings.

• Journal of Animal Environmental Science
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• v.18 no.3
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• pp.207-220
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• 2012

There are around 7,500 manure tanks to treat the manures from pigs in Korea. In the tank, there are too much sediments deposited on the base and wall, which causes low efficiency of stock capacity and manure fermentation. In order to minimize sediments and to ferment manure effectively, we developed a 2-fluid jet mixer for mixing sediments in liquid livestock manure tank. For developing the prototype, we tested a factorial experimental system with various nozzles, and simulated CFD models with two kinds of nozzle arrangement. From the results of factorial experiment and CFD simulation, we concluded the dia. ratio of primary : secondary nozzle should be 1:2 and the nozzles should be arranged at the same distances toward to the circumferential direction. With this results, we manufactured a 2-fluid jet mixer which is consists of four 2-phase nozzles, centrifugal slurry pump and root's type air blower. And, we carried out the performance test of the prototype in the round shaped liquid manure tank in the farm. The performance test results showed that the uniformity of TS (Total Solid) and VS (Volatile Solid) was raised from 21.3 g/L, 13.3 g/L In steady state to TS and VS to 23.0 g/L, 14.1 g/L in the mixing operation. Therefore, we could conclude that the prototype of 2-fluid mixer could make the solid material which could be sediments in the tank not to be deposited in the tank and to be contacted to air bubbles which could enhance the efficiency of the fermentation of livestock manure.

• Korean Journal of Environmental Biology
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• v.34 no.4
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• pp.223-232
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• 2016

Drought is one of important environmental stress for plant. Drought has deleterious effect to plant growth including maize (Zea mays L.) such as vegetative and/or reproductive growth, root extension, photosynthesis efficiency, flowering, anthesis-silking interval (ASI), fertilization, and grain filling. In this study, we screened drought tolerant maize in 21 cultivars from different sources, sixteen NAM parent lines (B73, CML103, CML228, CML247, CML277, CML322, CML333, CML69, Ki11, Ki3, Ky21, M37W, Mo18w, NC350, Oh43 and Tx303), four Korean hybrids (Cheongdaok, Gangdaok, Kwangpyeongok and Pyeonganok) and one Southeast Asian genotype (DK9955). Drought stress (DS) index was evaluated with leaf rolling score at seedling stage and ASI at silking date. The leaf rolling scoring of CML228, DK9955 and Ki11 were determined 1.28, 1.85, 1.86, respectively. However, M37W, Kwangpyeongok, B73 and NC350 were determined over the 3. ASI analysis revealed that CML228, CML103, Cheongdaok, NC350, B73, CML322, Kwangpyeongok and Ki11 are represented less than 5 days under DS and less than 3 days of difference between DS and well-watered (WW), but CML69, Ki3, Pyeonganok, M37W, Mo18w and Gangdaok were represented more than 10 days under DS and more than 8 days of difference between DS and WW. Multi-Dimensional Scaling (MDS) analysis determined CML228, Ki11, and CML322 were regarded as drought tolerance cultivars. Eventually, Ki11 showed genetic similarity with Korean cultivars by QTL analysis and MDS analysis. Ki11 has a potential for development of drought tolerance maize with Korean cultivars.

• Ecology and Resilient Infrastructure
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• v.5 no.3
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• pp.163-173
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• 2018

The biopolymer (BP) used in this study is mainly composed of xanthan gum and ${\beta}$-glucan derived from microorganism and has been introduced as a novel material for soil stabilization. However, the broad applicability of BP has been suggested in the field of geotechnical engineering while little information is available about the effects of BP on the vegetation. The goal of this study is to find the BP effects on the growth of Camelina sativa L. (Camelina) under drought condition. For more thorough evaluation of BP effects on the plant growth, we examined not only morphological but also physiological traits and gene expression patterns. After 25 days of drought treatment from germination in the soil amended with 0, 0.25, 0.5, and 1% BP, we observed that the BP concentration was strongly correlated the growth of Camelina. When plants were grown under drought stress, Camelina in 0.5% BP mixture showed better physiological parameters of the leaf stomatal conductance, electrolyte leakage and relative water content compared to those in control soil without BP. Plant recovery rate after re-watering was higher and the development of lateral root was lower in BP amended soil. RNA expression of Camelina leaf treated with/without drought for 7 and 10 days showed that aquaporin genes transporting solutes at bio-membrane, CsPIP1;4, 2;1, 2;6 and TIP1;2, 2;1, were induced more in the plants with BP amendment and drought treatment. These results suggest that the soil amended with BP has a positive effect on the transport of nutrients and waters into Camelina by improving water retention in soil under drought condition.

• Journal of the Korean earth science society
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• v.32 no.3
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• pp.276-293
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• 2011

In this study a modeling system consisting of Weather Research and Forecasting (WRF), Sparse Matrix Operator Kernel Emissions (SMOKE), the Community Multiscale Air Quality (CMAQ) model, and the CMAQ-Model of Aerosol Dynamics, Reaction, Ionization, and Dissolution (MADRID) model has been applied to estimate enhancements of $PM_{10}$ during Asian dust events in Korea. In particular, 5 experimental formulas were applied to the WRF-SMOKE-CMAQ (MADRID) model to estimate Asian dust emissions from source locations for major Asian dust events in China and Mongolia: the US Environmental Protection Agency (EPA) model, the Goddard Global Ozone Chemistry Aerosol Radiation and Transport (GOCART) model, and the Dust Entrainment and Deposition (DEAD) model, as well as formulas by Park and In (2003), and Wang et al. (2000). According to the weather map, backward trajectory and satellite image analyses, Asian dust is generated by a strong downwind associated with the upper trough from a stagnation wave due to development of the upper jet stream, and transport of Asian dust to Korea shows up behind a surface front related to the cut-off low (known as comma type cloud) in satellite images. In the WRF-SMOKE-CMAQ modeling to estimate the PM10 concentration, Wang et al.'s experimental formula was depicted well in the temporal and spatial distribution of Asian dusts, and the GOCART model was low in mean bias errors and root mean square errors. Also, in the vertical profile analysis of Asian dusts using Wang et al's experimental formula, strong Asian dust with a concentration of more than $800\;{\mu}g/m^3$ for the period of March 31 to April 1, 2007 was transported under the boundary layer (about 1 km high), and weak Asian dust with a concentration of less than $400\;{\mu}g/m^3$ for the period of 16-17 March 2009 was transported above the boundary layer (about 1-3 km high). Furthermore, the difference between the CMAQ model and the CMAQ-MADRID model for the period of March 31 to April 1, 2007, in terms of PM10 concentration, was seen to be large in the East Asia area: the CMAQ-MADRID model showed the concentration to be about $25\;{\mu}g/m^3$ higher than the CMAQ model. In addition, the $PM_{10}$ concentration removed by the cloud liquid phase mechanism within the CMAQ-MADRID model was shown in the maximum $15\;{\mu}g/m^3$ in the Eastern Asia area.

• Journal of Bio-Environment Control
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• v.26 no.3
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• pp.227-234
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• 2017

This study aimed to investigate the nutrient solution developed by based on nutrient-water absorption rate of strawberry 'Maehyang' by comparing growth and yield for 8 months with 5 kinds of nutrient solution with different ion composition. Strawberry plants were planted at elevated bed and supplied with five kinds of nutrient solutions (RDA), Yamazaki, PBG, University of Seoul (UOS) and NewUOS from one month onwards. Five types of nutrient solution were supplied to the strawberry plants associated with EC $1.0dS{\cdot}m^{-1}$, pH 6.0, $150{\sim}300mL{\cdot}plant^{-1}$ per day. At 60 days after planting, leaf width and leaf petiole of the strawberry plants showed significant differences among nutrient solution types and photosynthesis was higher in RDA and NewUOS nutrient solution and lower in PBG nutrient solution. The EC of the drainage on vegetative growth stage was $0.7{\sim}0.8dS{\cdot}m^{-1}$, which is lower than the supplied EC level, and to $1.0-1.2dS{\cdot}m^{-1}$, afterwards. The pH of the drainage was higher in Yamzaki solution as 6.2~6.8, while the pH of the UOS nutrient solution was lower in 5.1~5.2. Nitrate content was most absorbed in vegetative growth stage and after flower clusters development. The potassium uptake was highest at the NewUOS followed by UOS and Yamazaki nutrient solution. At six months after -planting fresh weight and dry weight of shoot and root were higher in UOS and NewUOS nutrient solution than other nutrient solutions, and the dry matter ratio was lower at 43.5% in Yamazaki nutrient solution and 30.6% in NewUOS nutrient solution than other solutions. Length, width, weight, and sugar content of the strawberries harvested from December to February were unaffected by treatment, but yield was higher in NewUOS nutrient solution due to increasing fruit number and average weight. From March to May, number of fruit was higher in Yamazaki nutrient solution. In conclusion, there was no difference in the growth of 'Maehyang' when 5 nutrient solutions were grown under hydroponics. But in order to improve the marketability, the NewUOS nutrient solution is appropriate to use from planting to February and it is suitable to use Yamazaki nutrient solution after March when temperature is high and the amount of fruit set per inflorescence.

• Journal of Bio-Environment Control
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• v.7 no.1
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• pp.43-54
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• 1998

This experiment was conducted to develop optimal nutrient solution for tomato plants, according to the plant growth stages in closed system. Perlite substrate was supplied with 1/2 and 1 strength of the solution of National Horticultural Research Station in Japan. Plants grew better and the nutrient_contents in the leaves were also proper in 1 strength. Based on these results, optimal nutrient solution in perlite was composed by n/w of 1 strength according to the plant growth rates ： N 13.5, P 3.3, K 7.0, Ca 7.0, Mg 3.5 me.L$^{-1}$ in seedling stage, N 14.2, P 3.3, K 8.0, Ca 7.5, Mg 4.0 me.L$^{-1}$ in vegetative stage and N 10.0, P 3.0, K 7.0, Ca 6.0, Mg 3.0 me.L$^{-1}$ in reproductive stage. To examine the suitability of the nutrient solution developed in this experiment, tomato plants were grown in rockwool and supplied with two different composition and concentration of nutrient solution composed by n/w of 1 strength in perlite (SCUT) and by Research Station for Greenhouse Vegetable and Floriculture on the Netherlands (PBG). PH and EC in SCUT were changed little in 1 strength but a significant change of PH was shown in 1/2 strength. Later, drained solutions in rockwool were also analyzed according to the Plant growth stages. Low concentrations of N and P in root zone were shown in early growth stage but N was increased in reproductive stage, while, K, Ca, Mg concentration was consistent through the whole growth stage. Considering these results, we found that the rebalance of N and P was needed, that is, reduction of N concentration in reproductive stage and increasing of P concentration in vegetative stage.