• Title/Summary/Keyword: root cuttings

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Use of Single-leaf Cutting in the Study of the Expression of Starch Synthesis and Modification Genes in Sweetpotato

  • Kim Sun-Hyung;Hamada Tatsuro;Otani Matoyasu;Koga Hironori;Shimada Takiko
    • Journal of Plant Biotechnology
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    • v.7 no.2
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    • pp.123-127
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    • 2005
  • The evaluation of source potential and sink strength is the generally large and laborious sample size required to adequately assess anyone of the parameters in field-grown sweetpotato. For this purpose we used the rooted single-leaf cuttings with petioles, because the source and sink organs are restricted in this system. The rooted single-leaf cutting of sweetpotato provides a unique source-sink model system, and is established within about 50 days after planting. In this study, the sink potential of sweetpotato tubers was examined based on the expression of genes for starch synthesis (AGPase) and modification (SBEII and GBSSI) in single rooted leaf plant. The gene expression patterns of GBSSI, SBEII and AGPase at various developmental stages and in different types of root tissues presented. These results suggest that the rooted single-rooted method can be used an ideal model system to study physiological and biochemical mechanisms in sweetpotato.

Several Factors Affecting to Rooting of Stem Cuttings in Rubus buergeri Miquel (삽목시기, 삽수 채취 부위 및 마디수가 겨울딸기 지삽의 발근에 미치는 영향)

  • Kang, Young-Kil;Ko, Mi-Ra;Kang, Si-Young;Riu, Key-Zung
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.3
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    • pp.77-80
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    • 2005
  • This study was conducted to determine effects of cutting date, cutting position, and node number on rooting of Rubus buergeri Miquel. Apical, medial, and basal parts of the vines emerged in 2002 were cut on June 26, August 2, and September 4, in 2002. Vine pieces with one to four nodes taken from the medial parts of the vines were also cut on July 10. The greatest rooting (69.6%) occurred in cuttings taken on August 2. Cutting taken on June 26 had the longest roots (23.1 cm). Rooting and new shoot growth markedly decreased at September 4 cutting. The greatest rooting (55.4%) occurred in cuttings taken from medial part of the vines and followed by apical parts (45.6%), and basal parts (25.4%). The other traits were not significantly affected by cutting position. Rooting percentage and percent new shoot growth were not significantly affected by node number, but root number and root dry weight per cutting, and new shoot growth increased with node number.

Hardwood Cutting with Callusing in the Mulberry(Morus bombycis Koidz.) I. Effect of a Root-Promoting Substance with Different Concentrations on Root Formation (뽕나무 유합촉진 고조삽목에 관한 연구 I. 발근촉진제 농도가 발근생장에 미치는 영향)

  • 최승운;김호락
    • Journal of Sericultural and Entomological Science
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    • v.33 no.2
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    • pp.63-67
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    • 1991
  • Various concentrations of ${\alpha}$-naphthalence acetic acid(NAA) as a root-promoting substance were tested in hardwood cutting of the mulberry(Morus bombycis Koidz., cultivar : Shinkwangppong) to make clear the callusing effect on the budding and root growth. Budding and shoot growth of cuttings were delayed at high concentrations of NAA within 10 days of callusing. Especially more severe is it at higher than 50ppm. More than 93% of them, however, budded in two weeks when callused at less than 100ppm NAA. Although rooting was accelerated at high concentration of NAA from the bigining of cutting, after that, rooting percentage increased to reach 100% in 35 days of cutting in any concentration except 150ppm with relatively low rooting. Root growth was utmostly accelerated at 50ppm NAA to show the highest amount in number, length and weight of roots per cutting although high concentration of it decreased mean root length.

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Effect of Inorganic Environmental Factors on the Growth on Pinus koraiensis Seedlings (II) - The Influence of Root cuttings on the Growth of Seedlings Grown on the Transplanting Bed - (무기적(無機的) 환경요인(環境要因)이 잣나무 유묘(幼苗)의 생육(生育)에 미치는 영향(影響)에 관(關)한 연구(硏究) (II) - 파종상(播種床)에서 피음(被陰) 처리(處理)한 유묘(幼苗)의 단근영향(斷根影響) -)

  • Kim, Young Chae
    • Journal of Korean Society of Forest Science
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    • v.76 no.1
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    • pp.1-10
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    • 1987
  • This study was carried out to examine the influence of shading and root-cutting on the survival and growth of Pinus koraiensis seedlings. 1, The highest survival percentage of seedlings transplanted to transplanting bed appeared at the plot of 19% in shading. The 50% of the seedlings were survived if half of them were root-cutting. 2. Shoot elongation, root growth and diameter increment were the best at the plot of 100% in relative light intensity. However, those were the best at the plot with 1/4 root cutting. 3. The increase of needle fascicle number and the growth of needle surface area per seedling were the longest at the plot of 63% relative light intensity. Among root cutting treatments the 1/4 cutting plot showed the largest growth of needle areas.

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Effect of Boron in Nutrient Solution on Root Development and Freezing Tolerance of Mulberry

  • Le, Quang-Tu;Keu
    • Journal of Sericultural and Entomological Science
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    • v.37 no.2
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    • pp.99-108
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    • 1995
  • The experiment was carried out to define the effect of the boron in nutrient solution on the development of mulberry root and cold damage. The length of shoots only attained about 50cm and then stopped in case of boron deficiency, while the mulberry trees developed vigorous and attained over 190cm long under boron supply condition. When boron is deficient, the measles appeared on surface of the stem and the necrosis appeared on the petiole, midribs and veins of leaves. At 70 days after planting, almost all new roots and old roots changed to brown, the lateral roots became necrosis by boron deficiency. After three days of stopping boron supply, many new roots changed to brown and after two days of boron resupply, new roots began to grow out and then new roots normally developed. Content of boron in leaves and barks increased by the increase of boron concentration in nutrient solution and maximum boron content in leaves and barks was obtained with boron-sufficient treatment of 0.5ppm. Treatment of 0.5ppm boron supply indicated that the leaves and barks contained more phospholipid, protein, sugar, RNA and proline than treatment of 0.01ppm boron supply in nutrient solution. The cuttings grown in boron supply nutrition have a sufficient tolerance at -10$^{\circ}C$ and -15$^{\circ}C$ for 24 hours while cuttings grown in boron-deficient nutrition have a weak tolerance at the same condition. As mentioned above, we can conclude that the effect of boron deficiency on root development is much severe and the relationship between the lack of boron and cold damage is very closely related.

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The Production of Adventitious Sprouts by Water-soak and Vegetative Propagation of Plus tree of Ash Species Through Cuttings of Their Sprouts (물푸레나무속(屬) 수형목(秀型木)의 수심(水沈)에 의(依)한 지조맹아(枝條萌芽) 발생력(發生力)과 이를 이용(利用)한 녹지삽목(綠枝揷木) 증식시험(增殖試驗))

  • Han, Sang Don;Hong, Sung Ho;Min, Young Taek;Kim, Young Mo;Kim, Hong Eun
    • Journal of Korean Society of Forest Science
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    • v.83 no.2
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    • pp.148-154
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    • 1994
  • As a vegetative propagation method for ash species, which is a dioecism and a long cycle of fructification, cut-stem was soaked in water to induce adventitious sprouts, and 2-year-old ash seedling was cut in a nursery to induce adventitious sprouts. We obtained the 1,019 adventitious sprouts from branches of 101 plus trees througout the country. The mean ortet-age is 48. There is not a correlation between ortet ages and production of adventitious sprouts. These sprouts were placed in a cutting bed for rooting. Root ability varied with environmental factors of cuttings. The best rooting(87%) resulted from cutting performed in a vinyl-plastic greenhouse. Rooting was better on perlite+peat moss(2 : 1) medium than other media tested. The rooting ability was generally higher in 2-year-old ortet than plus tree ortet. In the root development of cuttings the non-container cuttings was better than container cuttings.

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Propagation Efficiencies at Different LED Light Qualities for Leaf Cutting of Six Echeveria Cultivars in a Plant Factory System (에케베리아 6품종의 엽삽 시 식물공장시스템 내 LED 파장에 따른 번식 효율)

  • Kim, Seongmin;Kim, Jiseon;Oh, Wook
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.363-370
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    • 2018
  • The succulent plants of Echeveria genus are in increasing demand worldwide, but it is difficult to supply good quality young plants throughout the year because propagation efficiencies are depend on cultivar and environmental factors. This study was carried out to investigate the propagation efficiencies of leaf cutting in Echeveria cultivars at different LED light qualities in a closed-type plant factory system. Leaf cuttings cut from stock plants of six difficult-to-propagated cultivars 'Afterglow (AG)', 'Berkeley Light (BL)', 'Mason (MS)', 'Subsessilis Light (SL)', 'Cream Tea (CT)', and 'Ben Badis (BB)' were put into cutting media in the plant factory system maintained at a temperature of $24{\pm}2^{\circ}C$ and relative humidity of $60{\pm}10%$, and watered with over-head irrigation twice a week. Cuttings were irradiated with sole or mixed red (R, 660 nm), blue (B, 450 nm), green (G, 530 nm), and far-red (FR, 730 nm) LEDs as follows: R10, R8+B2, R5+B5, R7+B2+FR1, and R7+B2+G1. PPFD just above the cuttings was $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and photoperiod was 16/8 (light/dark) hours. As a result, propagation efficiencies were dependent on cultivar. Rooting and shooting were relatively easy in 'SL' but shoot formation in 'AG' was very difficult. Light qualities from LEDs also affected plant regeneration. Light conditions with a higher ratio of B, R5+B5, R7+B2+FR1, and R7+B2+G1, promoted shoot formation and growth but inhibited rooting and root growth. R10 and R8+B2 with a higher ratio of R promoted rooting and root growth and inhibited shoot formation and growth of cuttings. In addition, the treatment with FR increased leaf size and biomass of the all plants. Therefore, further studies are needed to investigate the optimum compositions of LED light quality for the improvement of leaf cutting efficiency in difficultto-propagated Echeveria cultivars.

The effect of soil heterogeneity and container length on the growth of Populus euramericana in a greenhouse study

  • Rahman, Afroja;Meng, Loth;Han, Si Ho;Seo, Gi Chun;Jung, Mun Ho;Park, Byung Bae
    • Korean Journal of Agricultural Science
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    • v.45 no.2
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    • pp.143-153
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    • 2018
  • Soil characteristics along with various container lengths have an important role in the early survival rate and growth of seedlings by influencing the seedling quality. This experiment was conducted to investigate the effect of container length and different soil mixtures on the growth of poplar in a greenhouse. Two types of soil, homogeneous vs. heterogeneous, were used along with two container lengths (30 vs. 60 cm). The heterogeneous soil was made by dividing 50% vermiculite from a mixture of 25% vermicompost and 25% nursery soil in volume. For the homogeneous soil, the above three soil types were mixed together. Populus euramericana clone cuttings were planted in late April, and then, the growth height, root collar diameter (RCD) and biomass were measured in August. The height of the poplar was not significantly affected by container length and soil type, but the RCD was significantly affected by soil type. Leaf and root biomass was higher at the long container than at the short container for both soil treatments, but stem biomass was lower at the heterogeneous soil than at the homogeneous soil treatment. Root to shoot biomass ratio was higher at the heterogeneous soil treatment than at the homogeneous soil treatment by 12%. In conclusion, heterogeneous soil along with a long container is suitable to increase the carbon allocation into the root.

Rooting and Survival Rate as Affected by Various Types and Concentrations of Auxin on 'Maehyang' Strawberry in Cutting Propagation ('매향' 딸기의 삽목 번식 시 옥신의 종류 및 처리 농도에 따른 발근율과 생존율)

  • Hwang, Hee Sung;Jeong, Hyeon Woo;Lee, Hye Ri;Jo, Hyeon Gyu;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.56-64
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    • 2021
  • This study was conducted to examine the effect by different types and concentrations of auxin on the rooting and growth of strawberry (Fragaria × ananassa Duch. cv. Maehyang) cuttings in the greenhouse. The NAD (1-naphthylacetamide), IBA (indole-3-butyric acid), and IAA (3-indoleacetic acid) were applied with a 1 hour soaking as 50, 100, 150, and 200 mg·L-1, respectively. The non-treatment was set as the control. The cuttings of strawberry were transplanted in the strawberry seedling tray filled with coir medium on June 4, 2020. The humidification was carried out for 2 weeks. The average relative humidity, daytime temperature, and nighttime temperature inside the humidification tunnel was 63.4 ± 15%, 29.3 ± 5℃, and 16.2 ± 5℃, respectively. There was no significant difference in rooting rate on the control, IBA, and IAA treatments. However, significantly low rooting rates were observed in NAD treatments. The survival rates were significantly higher in the control and IBA with 50 mg·L-1 than in other treatments. The number of leaves was the highest in IBA with 100 mg·L-1. The root length was the longest in the control. More number of roots were counted in IAA with 100 and 150 mg·L-1. The dry weight of root was the heaviest in the control. The total root length, root surface, number of root tips, and number of root forks were significantly higher in the control. As a result, control, IAA, and IBA showed similar shoot and root growth. However, NAD showed the worst root and shoot growth. Consequently, compared with IAA and IBA, NAD was not appropriate plant growth regulator of rooting for cutting propagated strawberries.

Growth and Tuber Yield of Sweet Potato Slips Grown under Different Light-Emitting Diodes (LED 광질에 따른 고구마의 묘소질 및 괴근 수량성)

  • Lee, Na Ra;Lee, Seung Yeob
    • Journal of Bio-Environment Control
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    • v.23 no.4
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    • pp.356-363
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    • 2014
  • This work was conducted to investigate the field growth and yield of the sweetpotato (Ipomoea batatas) slips grown under different light emitting diodes (LEDs). Sweet potato cuttings of 3 cultivars ('Matnami', 'Shinhwangmi', and 'Yeonhwangmi') were cultivated under fluorescent lamp (FL) and several LEDs (PPF $150{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ at 20cm distance) in deep flow culture system for 20 days. The plants were acclimatized under sunlight for 10 days, and then cuttings (30cm length) were planted with $75{\times}25cm$ planting density on June 10th, covered with black vinyl film during growth period. Length and diameter of vine, number of root were excellent in the red plus blue (7:3) LED than the other treatments. At 30 days after planting, the survival rate in red plus blue (7:3) LED was significantly higher than that in FL and red LED, and it was not different among cultivars. Vine length, vine diameter, and number of node were not significant among LED light qualities and cultivars. After 120 days in the field cultivation, vine length, vine diameter, number of node, number of branch, and fresh weight of shoot were not significant among LED light qualities, but those except the number of branch showed significant differences among cultivars. Yield characteristics among LED light colors were not significant, but weight of storage root per plant, mean weight of storage root, and yield showed significant differences among cultivars. The yield per 10a in 'Matnami', and 'Yeonhwangmi' was significantly higher than that in 'Shinhwangmi'.