• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1467-1470
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• 2012

We compared rapid fingerprinting using repetitive sequencebased PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.

• Journal of Plant Biology
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• v.39 no.1
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• pp.49-55
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• 1996

We have studied the DNA of Allium sativum L. with respect to highly repetitive sequences. Fast reassociated DNA fragments expected to be highly repetitive sequences based on $C_{o}t$ curve were isolated and characterized. Their copy numbers were approximately $10^{5}~10^{7}$ per haploid genome. Nucleotide sequences analysis of six candidates reveals that their G/C content were low, 25-40% and typical patterns of repeating sequences exist. Repeat sequences were used as probes to access restriction fragment length polymorphism (RFLP) of genomic DNAs of four local clones, Tanyang, Mungyong, So san, and Uisong. The hybridization pattern were very similar among these four local clones.clones.

• 제어로봇시스템학회:학술대회논문집
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• 1988.10a
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• pp.326-331
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• 1988

In this paper, an iterative leaning control algorithm for the linear discrete system is proposed. Based upon the parameter estimation method, the learning for good tracking control is acqured through a sequence of repetitive operations. A series of simulation are performed to show the validity of this algorithm.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.12
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• pp.3152-3165
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• 2012

Wireless sensor networks (WSN) often employ asynchronous MAC scheduling, which allows each sensor node to wake up independently without synchronizing with its neighbor nodes. However, this asynchronous scheduling may not deal with collisions due to hidden terminals effectively. Although most of the existing asynchronous protocols exploit a random back-off technique to resolve collisions, the random back-off cannot secure a receiver from potentially repetitive collisions and may lead to a substantial increase in the packet latency. In this paper, we propose a new collision resolution algorithm called Transient Coordinator (TC) for asynchronous WSN MAC protocols. TC resolves a collision on demand by ordering senders' transmissions when a receiver detects a collision. To coordinate the transmission sequence both the receiver and the collided senders perform handshaking to collect the information and to derive a collision-free transmission sequence, which enables each sender to exclusively access the channel. According to the simulation results, our scheme can improve the average per-node throughput by up to 19.4% while it also reduces unnecessary energy consumption due to repetitive collisions by as much as 91.1% compared to the conventional asynchronous MAC protocols. This demonstrates that TC is more efficient in terms of performance, resource utilization, and energy compared to the random back-off scheme in dealing with collisions for asynchronous WSN MAC scheduling.

• Journal of Dairy Science and Biotechnology
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• v.38 no.4
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• pp.169-188
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• 2020

Salmonella spp. is the most common cause of gastrointestinal food poisoning worldwide, and human salmonellosis is mostly caused by the consumption of contaminated food. Therefore, the development of rapid detection methods for Salmoenlla spp. and rapid identification of the source of infection by subtyping are important for the surveillance and monitoring of food-borne salmonellosis. Therefore, this review introduces (1) History and nomenclature of Salmoenlla spp., (2) Epidemiology of Salmoenlla spp., (3) Detection methods for Salmoenlla spp. - conventional culture method, genetic detection method, molecular detection methods, and aptamer, and (4) Subtyping methods for Salmoenlla spp. - pulsed-field gel electrophoresis and repetitive sequence-based polymerase chain reaction (PCR).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.4
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• pp.352-358
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• 2011

The presence of ISKNV-like viruses in various freshwater ornamental fish species imported from Asia was confirmed by polymerase chain reaction(PCR) amplification of the ATPase(adenosine triphosphatase) gene. Interestingly, molecular analyses of the Open Reading Frame 25(ORF25) region of these isolates based on the ISKNV(Infectious spleen and kidney necrosis virus) genome revealed the presence of various repetitive sequences. ORF25 repeat sequence length had no effect on cumulative mortality of rock bream Oplegnathus fasciatus challenged with tissue homogenates of infected pearl gourami, Trichogaster leeri; silver gourami, Trichogaster microlepis; blue gourami, or Trichogaster trichopterus. All isolates induce cumulative mortalities after 12 days of infection, confirming that ORF25 polymorphism did not affect the pathogenicity of ornamental fish megalocytiviruses that cross infect rock bream, a seawater fish. Also, no statistically significant differences in spleen index or viral copy number in infected tissues was detected between isolates with varying ORF25 repeat sequence lengths. However, further studies are necessary to fully characterize the functional characteristics of these polymorphisms in megalocytivirus disease in ornamental fishes.

• International Journal of Internet, Broadcasting and Communication
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• v.12 no.2
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• pp.45-50
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• 2020

A Language model with neural networks commonly trained with likelihood loss. Such that the model can learn the sequence of human text. State-of-the-art results achieved in various language generation tasks, e.g., text summarization, dialogue response generation, and text generation, by utilizing the language model's next token output probabilities. Monotonous and boring outputs are a well-known problem of this model, yet only a few solutions proposed to address this problem. Several decoding techniques proposed to suppress repetitive tokens. Unlikelihood training approached this problem by penalizing candidate tokens probabilities if the tokens already seen in previous steps. While the method successfully showed a less repetitive generated token, the method has a large memory consumption because of the training need a big vocabulary size. We effectively reduced memory footprint by encoding words as sequences of subword units. Finally, we report competitive results with token level unlikelihood training in several automatic evaluations compared to the previous work.

• Korean Journal Plant Pathology
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• v.10 no.1
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• pp.1-6
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• 1994

Randomly chosen recombinant clones of Fusarium oxysporum were analysed to select useful probes for relatedness analysis of Fusarium oxysporum. Genomic DNA of F. oxysproum f. sp. cubense, digested with HindIII, was ligated to pUC118 and used to transform Escherichia coli strai DH5$\alpha$. Three clones were identified that hybridized to mutiple restriction fragments of some formae speciales of F. oxysporum. These probes detected repetitive sequences in HindIII or EcoRI digested DNAs. Repeated copy clone pFC46, pFC52 and pFC54 showed evident polymorphisms among ten formae speciales of this fungus. Since clone pFC 52 strongly hybridized to multiple EcoRI-digested restriction fragments of f. sp. cubense, it may be useful as a probe for analysis of other genetic characteristics of this forma specialis. The results suggest that our clones might be very useful as probes for relatedness analysis between or within formae speciales of Fusarium oxysporum.

• Journal of the Korean Society of Safety
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• v.27 no.3
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• pp.131-140
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• 2012

Though there might exist not a few differences between cyclic works and atypical works, many researchers have applied the same assessment techniques that used for repetitive works, which may result introduce bias in their conclusions. This research aimed to verify whether there exist non-negligible work characteristics and/or dissimilarity among works with different work nature and whether one of the most prevalent assessment techniques for assessing ergonomic hazards of musculoskeletal disorders, REBA, can be applied to atypical works. For a general hospital, an automobile repair shop, and two auto-part assembly plants which manufactures quite different parts, a questionnaire survey and field investigation and ergonomic assessment were carried out and analyzed statistically with reference to the 3rd Quantification technique. The results showed that there exist remarkable difference between physical factors in cyclic works and atypical non-cyclic works. As for repetitive work, body posture was significant factors affecting on musculoskeletal disorders while atypical works seemed to have none which implied that the necessity of taking psychosocial factors into account for assessment of hazards. Complain rate in repetitive works was highest shoulder, back, and neck or wrist in sequence. However, there existed no consistent trend in complain rate in atypical works. And, though weight of manufacturing objects was a common factor that can partly explain musculoskeletal complain, time duration was significant in atypical work whereas repeatability and body posture were significant in repetitive works. As being the results, to summarize, it could be said that application of conventional ergonomic assessment techniques regardless of repetitiveness would be fruitless, and that the necessity of a unique methodology focused on atypical non-cyclic works should not be neglected.

• Journal of Preventive Medicine and Public Health
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• v.45 no.1
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• pp.1-7
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• 2012

Using three Austrian case studies, the variegated applications of molecular typing in today's public health laboratories are discussed to help illustrate preventive management strategies relying on DNA subtyping. DNA macrorestriction analysis by pulsed field gel electrophoresis has become the gold standard for subtyping of food borne pathogens like listeria, salmonella, campylobacter and Bacillus cereus. Using a Salmonella Mbandaka outbreak from the year 2010 as example, it is shown how the comparison of patterns from human isolates, food isolates, animal isolates and feed isolates can allow to identify and confirm a source of disease. An epidemiological connection between the simultaneous occurrence of tuberculosis in cattle and deer with cases of human tuberculosis due to Mycobacterium caprae in 2010 was excluded using mycobacterial interspersed repetitive units variable-number tandem repeats subtyping. Also in 2010, multilocus sequence typing with nonselective housekeeping genes, the so-called sequence based typing protocol, was used to elucidate connections between an environmental source (a hospital drinking water system) and a case of legionellosis. During the last decades, molecular typing has evolved to become a routine tool in the daily work of public health laboratories. The challenge is now no longer to simply type microorganisms, but to type them in a way that allows for data exchange between public health laboratories all over the world.