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http://dx.doi.org/10.5657/KFAS.2011.0352

Characterization of the Repetitive Sequences Present in the ORF25 Genomic Region of Megalocytiviruses from Ornamental Fishes  

Jin, Ji-Woong (Department of Aquatic Life Medicine, Pukyong National University)
Nam, Jeong-Hee (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute)
Kim, Kwang-Il (Department of Aquatic Life Medicine, Pukyong National University)
Hong, Su-Hee (Department of Marine Biotechnology, Kangnung-Wonju National University)
Byon, Ju-Young (Hydro and Nuclear Power Co., Ltd., Environmental Management Team Safety & Environment Department)
Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.44, no.4, 2011 , pp. 352-358 More about this Journal
Abstract
The presence of ISKNV-like viruses in various freshwater ornamental fish species imported from Asia was confirmed by polymerase chain reaction(PCR) amplification of the ATPase(adenosine triphosphatase) gene. Interestingly, molecular analyses of the Open Reading Frame 25(ORF25) region of these isolates based on the ISKNV(Infectious spleen and kidney necrosis virus) genome revealed the presence of various repetitive sequences. ORF25 repeat sequence length had no effect on cumulative mortality of rock bream Oplegnathus fasciatus challenged with tissue homogenates of infected pearl gourami, Trichogaster leeri; silver gourami, Trichogaster microlepis; blue gourami, or Trichogaster trichopterus. All isolates induce cumulative mortalities after 12 days of infection, confirming that ORF25 polymorphism did not affect the pathogenicity of ornamental fish megalocytiviruses that cross infect rock bream, a seawater fish. Also, no statistically significant differences in spleen index or viral copy number in infected tissues was detected between isolates with varying ORF25 repeat sequence lengths. However, further studies are necessary to fully characterize the functional characteristics of these polymorphisms in megalocytivirus disease in ornamental fishes.
Keywords
Polymorphism; Repetitive sequences; Freshwater ornamental fish; Megalocytivirus;
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