한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제44권4호
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  • Pages.352-358
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  • 2011
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
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관상어로부터 분리한 Megalocytiviruses에서 나타나는 ORF25 유전자 부위의 반복서열 특성 분석

Characterization of the Repetitive Sequences Present in the ORF25 Genomic Region of Megalocytiviruses from Ornamental Fishes

  • 진지웅 (부경대학교 수산생명의학과) ;
  • 남정희 (국립수산과학원 수산생물방역과) ;
  • 김광일 (부경대학교 수산생명의학과) ;
  • 홍수희 (강릉원주대학교 해양생물공학과) ;
  • 변주영 (한국수력원자력(주) 안전처민원환경팀) ;
  • 정현도 (부경대학교 수산생명의학과)
  • Jin, Ji-Woong (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Nam, Jeong-Hee (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Kim, Kwang-Il (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Hong, Su-Hee (Department of Marine Biotechnology, Kangnung-Wonju National University) ;
  • Byon, Ju-Young (Hydro and Nuclear Power Co., Ltd., Environmental Management Team Safety & Environment Department) ;
  • Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2011.04.08
  • 심사 : 2011.07.04
  • 발행 : 2011.08.30
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초록

The presence of ISKNV-like viruses in various freshwater ornamental fish species imported from Asia was confirmed by polymerase chain reaction(PCR) amplification of the ATPase(adenosine triphosphatase) gene. Interestingly, molecular analyses of the Open Reading Frame 25(ORF25) region of these isolates based on the ISKNV(Infectious spleen and kidney necrosis virus) genome revealed the presence of various repetitive sequences. ORF25 repeat sequence length had no effect on cumulative mortality of rock bream Oplegnathus fasciatus challenged with tissue homogenates of infected pearl gourami, Trichogaster leeri; silver gourami, Trichogaster microlepis; blue gourami, or Trichogaster trichopterus. All isolates induce cumulative mortalities after 12 days of infection, confirming that ORF25 polymorphism did not affect the pathogenicity of ornamental fish megalocytiviruses that cross infect rock bream, a seawater fish. Also, no statistically significant differences in spleen index or viral copy number in infected tissues was detected between isolates with varying ORF25 repeat sequence lengths. However, further studies are necessary to fully characterize the functional characteristics of these polymorphisms in megalocytivirus disease in ornamental fishes.

키워드

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