Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Comparison of Multilocus Sequence Typing (MLST) and Repetitive Sequence-Based PCR (rep-PCR) Fingerprinting for Differentiation of Campylobacter jejuni Isolated from Broiler in Chiang Mai, Thailand

  • Patchanee, Prapas (Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University) ;
  • Chokboonmongkol, Chomporn (Animal Health and Technical Service Office, Bangkok Agro-Industrial Products Public CO., LTD.) ;
  • Zessin, Karl-Hans (Department Panel, Veterinary Public Health, Free University Berlin) ;
  • Alter, Thomas (Institute of Food Hygiene, Free University Berlin) ;
  • Pornaem, Sarinya (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency) ;
  • Chokesajjawatee, Nipa (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency)
  • Received : 2011.12.21
  • Accepted : 2012.06.25
  • Published : 2012.11.28
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Abstract

We compared rapid fingerprinting using repetitive sequencebased PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.

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References

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