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http://dx.doi.org/10.4014/jmb.1112.12049

Comparison of Multilocus Sequence Typing (MLST) and Repetitive Sequence-Based PCR (rep-PCR) Fingerprinting for Differentiation of Campylobacter jejuni Isolated from Broiler in Chiang Mai, Thailand  

Patchanee, Prapas (Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University)
Chokboonmongkol, Chomporn (Animal Health and Technical Service Office, Bangkok Agro-Industrial Products Public CO., LTD.)
Zessin, Karl-Hans (Department Panel, Veterinary Public Health, Free University Berlin)
Alter, Thomas (Institute of Food Hygiene, Free University Berlin)
Pornaem, Sarinya (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency)
Chokesajjawatee, Nipa (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.11, 2012 , pp. 1467-1470 More about this Journal
Abstract
We compared rapid fingerprinting using repetitive sequencebased PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.
Keywords
MLST; rep-PCR; Campylobacter; broiler;
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