• 한국환경과학회지
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• 제23권12호
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• pp.2129-2134
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• 2014

The antioxidant activities and total phenolics of four Rosaceae species Pourthiaea villosa (Thunb.) Decne, Sorbus commixta Hedlund, Sorbaria sorbifolia var. stellipila Maxim and Pyrus pyrifolia (Burm.f.) Nakai were determined. Phenolic content (polyphenol and flavonoid), radical scavenging activities [2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylnezthiazoline-6-sulfoic acid) (ABTS)] and ferrous ion chelating effect were evaluated. Total polyphenol and flavonoid contents were highest in Pourthiaea villosa (Thunb.) Decne and lowest in Pyrus pyrifolia (Burm.f.) Nakai. Phenolic contents of Pourthiaea villosa (Thunb.) Decne was $331.45{\pm}7.78$ and $90.4{\pm}3.5mg{\cdot}g^{-1}$. DPPH and ABTS radical scavenging activities were found to be lowest in Sorbus commixta Hedlund whereas Sorbaria sorbifolia var. stellipila Maxim and Pourthiaea villosa (Thunb.) Decne showed relatively good DPPH and ABTS radical scavenging activities. Ferrous ion chelating effect was highest in Pyrus pyrifolia (Burm.f.) Nakai ($1.05{\pm}0.04mg{\cdot}ml^{-1}$) and lowest in Sorbus commixta Hundlund ($4.22{\pm}0.71mg{\cdot}ml^{-1}$).

• 한국식품영양과학회지
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• 제24권2호
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• pp.299-304
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• 1995

Antioxidative effect of tea extracts from green tea(steamed, roasted), oolong tea and black tea was investigated. Antioxidative activities of the crude catechin fraction were the most effective in oolong tea which contained the highest level of (-)-epigallocatechin gallate(EGCg). The water-soluble fraction obtained from oolong tea exhibited binding with more ferrous than copper ions. It showed a synergistic effect when used with an antioxidant such as BHT(0.5mg) and $\alpha$-tocopherol(2mg). Especially, this synergistic effect was exhibited more with BHT than with $\alpha$-tocopherol. Also steamed green tea, roasted green tea and oolong tea showed remarkable free radical scavenging action. The SC50(the concentration of a water-soluble fraction which is required to scavenge 50% of 100$\mu$M 1, 1-diphenyl-2-picryl hydrazyl radicals) of steamed and roasted green tea, oolong tea and black tea extracts were 11.3$\mu\textrm{g}$/ml, 11.4$\mu\textrm{g}$/ml, 12.7 $\mu\textrm{g}$/ml and 14.9$\mu\textrm{g}$/ml, respectively. It is assumed that the antioxidative activity of tea extracts is due to inhibition of peroxidation free radical scavenging and binding action of ferrous ions by mainly tea polyphenol compounds.

• Food Science and Biotechnology
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• 제18권4호
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• pp.849-854
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• 2009

Water extracts of injinssuk (Artemisia iwayomogi Kitamura) (WE) were obtained from the dried and ground leaves and stems of injinssuk. The WE was further fractionated into crude polysaccharide (C-PS) and nonpolysaccharide fractions (N-PS). The protective activities against the tert-butyl hydro peroxide induced mutangenecity on Escherichia coli PQ37 and reactive oxygen species scavenging activity of the WE, C-PS, and N-PS were studied. The WE obtained from leaves showed a significantly higher inhibitory effect on the mutagenicity than WE from stem. The WE obtained from the leaves having higher crude polysaccharide content but lower content of total carbohydrates had significantly higher antimutagenicity than that from the leaves with lower crude polysaccharide but higher total carbohydrate contents. Further study showed that C-PS fraction showed markedly stronger antimutagenic effect than N-PS. C-PS was also more effective than N-PS for hydroxyl radical scavenging activity, but was similar to N-PS in superoxide radical scavenging activity.

• KSBB Journal
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• 제31권4호
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• pp.200-207
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• 2016

In the present study, antioxidant activity of crude extract and its solvent-partitioned subfractions (n-hexane, 85% aqueous methanol, n-butanol, and water) obtained from Atriplex gmelinii was investigated using several different antioxidant assays. The tested samples possessed different antioxidant and radical-scavenging activities in different assays. n-butanol fraction showed the most potent radical-scavenging activity on reducing power while 85% aqueous methanol fraction exhibited the highest radical-scavenging activity on DPPH radicals and intracellular reactive oxygen species (ROS). On the otherhand, n-BuOH and 85% aqueous methanol revealed the similar inhibitory effect on peroxynitrite-scavenging and genomic DNA oxidation. These results suggest that the Atriplex gmelinii can be used as the valuable source for developing a natural antioxidant.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.462-466
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• 1994

Radical scavenging principles on 1, 1-diphenyl-2-picylhydrazyl(DPPH) radical were isolated from the seeds of Cassis tora L. Assignments of the^1H-{\;}and{\;}^{13}C-NMR$ data showed the active components to be an anthraquinone, alatemin nad two naphthopyrone glycosides, nor-rubro-fusarin-6-.betha.-D-glucoside(cassiaside) and rubrofusarin-6- -D-gentiobioside. Altemin showed more potent radical sacvenging effect than the others.

• Advances in Traditional Medicine
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• 제10권4호
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• pp.310-318
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• 2010

Verbena officinalis (Verbenaceae) has been used as herbal medicine or health supplement in both Western and Eastern countries for centuries. In the present study, we examined the anti-inflammatory and antioxidant activities of the methylene chloride fraction of V. officinalis (VMC). To elucidate the anti-inflammatory properties of VMC, we investigated the inhibition effects of nitric oxide production in interferon-gamma (IFN-$\gamma$) and lipopolysaccharide-stimulated mouse peritoneal macrophages. VMC suppressed nitric oxide production, inducible nitric oxide synthase and cyclooxygenase-2 expression dose-dependently without notable cytotoxicity. In various radical scavenging assays, VMC exhibited strong scavenging effect on DPPH radical, superoxide radical, nitric oxide radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical. VMC also showed potent reducing power. These findings strongly suggest that VMC may be beneficial in oxidative stress-mediated inflammatory disorders.

• Journal of Life Science
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• 제12권2호
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• pp.75-79
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• 2002

Tea catechins, the most important compounds in tea polyphenols, can efficiently scavenge superoxide anion free-radical ($O_2$.), hydroxyl radical. (.OH) The mechanism of scavenging active oxygen free radicals was investigated by ESR spin trapping technique and Chemiluminescence. Results showed that various tea catechins constitute an antioxidant cycle in accordance with the decreasing order of the first reductive potential, and produce the effect of cooperative strength each other. Esterificated catechins could scavenge active oxygen free radicals more effectively than the non-esterificated ones. When.OH and $O_2$.- were scavenged by (-)-epigallocatechin gallate [(-)- EGCG], the stoichiometric factors were 6, and the rate constants of scavenging reaction reached $7.71{\times}10^6$ and $3.52{\times}10^{11}$ L $mmol^{-1}s^{-1}$, respectively. In the mean time, tea catechins could scavenge superoxide anion fiee radical ($O_2$-.) and hydroxyl radical (.OH) in a dose dependent manner. But at higher concentration or pH value, tea catechins can induce the prooxidant.

• 한국식품위생안전성학회지
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• 제17권2호
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• pp.106-116
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• 2002

양강추출물 (Alpinia officinarum, 70% ethanol extract)과 함유 주성분인 galangirn은 프리라디칼소거작용과 지질과산화억제활성을 나타내었으며, $H_2O$$_2$ 또는 KO$_2$유도 세포독성에 대해서도 억제적으로 작용하여 세포보호효과를 나타내었다. DNA single strand breakage와 같은 산화적 스트레스에 대해서도 보호작용을 하고 있다. 또한, 마우스 소핵시험에 의하여 adriamycin과 같은 superoxide 우발물질에 의한 염색체 수준에서의 손상에 대해서도 억제효과를 나타내었다. 양강추출물은 galangin을 유효성분으로하여 산소프리라디칼들에 의한 산화적 손상에 억제적으로 작용하는 기전으로 산화적 스트레스에 대한 항노화, 암예방제로서의 응용가능성이 높은 추출물로서 향후 기능성 식품으로서의 응용가능성이 높을 것으로 판단되었다.

• 한국식품과학회지
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• 제35권1호
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• pp.121-124
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• 2003

본 연구는 거봉종 포도의 종자와 과피로부터 에탄올용매의 농도와 추출 온도변화에 의한 추출물의 수율의 차이와 그에 따른 free radical 소거 활성을 비교하였다. 각 분획물은 에탄올 추출물로부터 hexane 분획물, chloroform 분획물, ethyl acetate 분획물, butanol 분획물 및 물 분획물로 나누어 free radical 소거 활성을 $RC_{50}$으로 타나내었다. 추출 수율은 종자 및 과피 모두에서 추출 온도 $78^{\circ}C$, 에탄올 농도 100%에서 36.0%와 49.5%로 다른 온도와 농도 조건에 비하여 높은 수율을 나타내었다. 각 분획물 중에서는 물 분획물이 종자에서는 72.1%인데 비하여 과피에서는 92.7%로 다른 분획층에 비하여 많이 차지하였다. DPPH법에 의한 free radical 소거 활성에서는 종자의 경우, $78^{\circ}C$ 에탄올 추출물이 $RC_{50}\;=\;409.7\;{\mu}g/mL$이며, 분획물에서는 ethyl acetate층이 $RC_{50}\;=\;136.7{\mu}g/mL$로 가장 높은 활성을 나타내었다. 과피의 경우, $78^{\circ}C$ 에탄올 추출물이 $RC_{50}\;=\;6528.8\;{\mu}g/mL$로, 분획물에서는 ethyl acetate층이 $RC_{50}\;=\;694.7\;{\mu}g/mL$로 가장 높은 활성을 나타내었으나, 물 분획물은 free radical 소거 활성을 보이지 않았다. 이상의 결과를 통하여 추출온도와 용매 온도에 따른 수율의 차이가 나타났으며 추출온도와 free radical 소거 활성의 효능은 별다른 차이가 없는 것으로 나타났다. 전반적으로, 본 연구 결과 종자 에탄올 추출물과 분획물 모두 과피 추출물보다 현저히 높은 활성을 나타내었다.

• 대한한의진단학회지
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• 제17권3호
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• pp.275-286
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• 2013

In the present study, antioxidant activity and protective effect of extracts from Sambucus williamsii var. coreana stems (SWC) were evaluated on tert-butyl hydroperoxide (t-BHP) induced oxidative stress in human liver (Chang) cells. Antioxidant activities of the SWC extracts were determined by various radical scavenging activities, such as DPPH, ferric reducing antioxidant power (FRAP), 2,2'-azinobis-(3-ethybenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and oxygen radical absorbance capacity (ORAC) assay. SWC extracts showed strong antioxidant effect on various assay. To determine the hepatoprotective effects of SWC on t-BHP induced oxidative damage, cell viability was measured using MTT assay. Pretreatment of SWC extracts showed increasing cell viability, decreasing ROS and restoring mitochondria membrane potential on t-BHP induced oxidative stress in Chang cells. Our findings suggest that SWC extracts may be considered a potential agent for therapeutic protective effect from oxidative stress through its antioxidant activity.