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Protection of ROS-induced cytotoxicity and DNA damage by the extract of Alpinia of ficinarum  

이승철 (강원대학교 약학대학)
신경승 (경인지방 식품의약품 안전청)
허문영 (강원대학교 약학대학)
Publication Information
Journal of Food Hygiene and Safety / v.17, no.2, 2002 , pp. 106-116 More about this Journal
Abstract
The 70% ethanol extract of Alpinia officinarum and its major flavonoid, galangin showed strong antioxidative effect on the lipid peroxidation of ethyl linolate with Fenton's reagent and free radical scavenging effect to DPPH radical generation. However, they did not reveal any pro-oxidant effect on bleomycin-Fe(III) dependent DNA degradation. They also showed the protective effect against $H_2O$$_2$, KO$_2$ or UV-induced cytotoxicity in mammalian cells. They also showed the suppressive effect of DNA damage induced by $H_2O$$_2$ or KO$_2$ with dose-dependent manner in single cell gel electrophoresis(SCGE) assay. On the other hand, they have an anticlastogenic effect against adriamycin-induced micronucleated reticulocyte in peripheral blood of mice. These results suggest that the mechanism of inhibition by 70% ethanol extract of Alpinia officinarum and galangin against reactive oxygen species (ROS)-induced genotoxicity or cytotoxicity is due, at least partly, to their antioxidative and free radical scavenging properties without pro-oxidant effect. All these results indicate that 70% ethanol extract of Alpinia officinarum and galangin may be useful for protection against ROS-induced cytotoxicity and DNA damage.
Keywords
Alpinia officinarum; galangin; reactive oxygen species; antioxldative effect; free radical scavenging effect; DNA damage; chernoprevention;
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