• Title/Summary/Keyword: psaH

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Molecular Cloning and Characterization of a cDNA for the PSI-H Subunit Homolog of Photosystem I in Chinese Cabbage (배추로부터 광계 I의 PSI-H Subunit Homolog의 클로닝 및 분자생물학적 특성 연구)

  • Cha, Joon-Yung;Choi, Young-Jin;Lee, Hyo-Shin;Kim, Ki-Yong;Park, Geun-Je;Jo, Jin-Ki;Son, Dae-Young
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.1
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    • pp.51-58
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    • 2002
  • PSI-H is an intrinsic membrane protein known to be essential for efficient electron flow in PSI complex. We isolated a cDNA clone encoding a PSI-H subunit homolog from Chinese cabbage (Brassica campestris L.). The cDNA, designated bpsaH, had an insert of 435 bp and a full open reading frame that would encode a protein of 145 amino acids. The amino acid sequence deduced from the cDNA sequence is 79.3% identical to that of spinach, suggesting the cDNA most likely encodes Chinese cabbage PSI-H subunit. The bpasH was expressed at high level in leaf tissue and low level in flower bud, whereas it was undetectable in root tissue.

The Effects of Crosslinking Agent and pH Controlling Agent on Adhesive Properties of Water Soluble Acrylic PSA (가교제 및 pH 조절제가 수용성 아크릴 점착제의 점착 물성에 미치는 효과)

  • Seo, In-Seon;Park, Myung Chul;Lee, Myung Cheon
    • Journal of Adhesion and Interface
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    • v.4 no.3
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    • pp.9-13
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    • 2003
  • In this paper, the effects of crosslinking agent and pH controlling agent on adhesive properties and water solubility of water soluble acrylic pressure sensitive adhesives were studied by adding the crosslinking agents or pH controlling agents after the polymerization. the tack, cohesive strength, and peel strength were measured to evaluate adhesive properties. the turbidity was also measured to evaluate water solubility of pressure sensitive adhesives. When crosslinking agent was added less than 0.2 wt%, tack increased and cohesive strength and peel strength decreased with increasing concentration. When crossinking agent was added more than 0.2 wt%, the results were opposited. When pH controlling agent was added, tack and water solubility decreased and peel strength and cohesive strength increased with increasing concentration. the influence of pH controlling agents on adhesive properties and water solubility of pressure sensitive adhesive increased in the order of lithium hydroxide, potassium hydroxide and sodium hydroxide.

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Conceptual design for the Production of Hydrogen in Coal Gasification System (석탄 가스화에 의한 수소 제조공정 개념설계)

  • Lee, Yun-Ju;Na, Gi-Pung;Park, Moon-Ju;Lee, Sang-Deuk;Hong, Suk-In;Moon, Dong-Ju
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.10a
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    • pp.258-261
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    • 2008
  • 상용공정 모사기인 PRO-II를 이용하여 석탄 가스화에 의한 수소 제조공정 개념설계를 수행 하였다. 이 공정은 공기분리(ASU), 석탄가스화, 가스정제, 고온 WGS 반응, 저온 WGS 반응, 수분제거, $H_2$분리, $CO_2$ 분리, $CH_4$ 분리(PSA) 등으로 구성되어 있다. 가스화기의 모사조건은 온도 $1200{\sim}1500^{\circ}C$, 압력 $15{\sim}30atm$, 공급몰비 C:$H_2O$:$O_2$=1:0.5$\sim$1:0.25$\sim$0.5로 하였으며, 정제공정의 온도와 압력은 각각 $550^{\circ}C$, 24.5atm으로 하였다. 생성된 합성가스는 WGS(HTS($400^{\circ}C$, 24atm), LTS($250^{\circ}C$, 23.5atm)) 반응을 거쳐 고순도 수소로 분리정제된다. 석탄을 10ton/day으로 공급하였을 때, 804.0kmol/day의 수소가 생성되었으며, 이때 가스화기 조건은 $1500^{\circ}C$, 25atm, 공급몰비 C:$H_2O$:$O_2$ = 1:0.58:0.43이었다.

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Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites

  • Park, Hana;Yoon, Chang Young;Kim, Jin Sook;Kim, Joo-Hwan
    • Korean Journal of Plant Resources
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    • v.28 no.6
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    • pp.743-751
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    • 2015
  • Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.

Hydrogenation Properties of MgH2-CaO Composites Synthesized by Hydrogen-Induced Mechanical Alloying

  • Kim, Min Gyeom;Han, Jeong-Heum;Lee, Young-Hwan;Son, Jong-Tae;Hong, Tae Whan
    • Korean Journal of Metals and Materials
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    • v.56 no.11
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    • pp.829-834
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    • 2018
  • Although magnesium-based alloys are attractive materials for hydrogen storage applications, their activation properties, hydrogenation/dehydrogenation kinetics, thermodynamic equilibrium parameters, and degradation characteristics must be improved for practical applications. Further, magnesium poses several risks, including explosion hazard, environmental pollution, insufficient formability, and industrial damage. To overcome these problems, CaO-added Mg alloys, also called Eco-Mg (environment-conscious Mg) alloys, have been developed. In this study, $Eco-MgH_x$ composites were fabricated from Mg-CaO chips by hydrogen-induced mechanical alloying in a high-pressure atmosphere. The balls-to-chips mass ratio (BCR) was varied between a low and high value. The particles obtained were characterized by X-ray diffraction (XRD), and the absorbed hydrogen was quantified by thermogravimetric analysis. The XRD results revealed that the $MgH_2$ peaks broadened for the high BCR. Further, PSA results revealed particles size were decreased from $52{\mu}m$ to $15{\mu}m$.

Modeling, Simulation and Optimization of Hydrogen Production Process from Glycerol using Steam Reforming (글리세롤로부터 수증기 개질에 의한 수소 생산공정의 모델링, 시뮬레이션 및 최적화)

  • Park, Jeongpil;Cho, Sunghyun;Lee, Seunghwan;Moon, Dong Ju;Kim, Tae-Ok;Shin, Dongil
    • Korean Chemical Engineering Research
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    • v.52 no.6
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    • pp.727-735
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    • 2014
  • For improved sustainability of the biorefinery industry, biorefinery-byproduct glycerol is being investigated as an alternate source for hydrogen production. This research designs and optimizes a hydrogen-production process for small hydrogen stations using steam reforming of purified glycerol as the main reaction, replacing existing processes relying on steam methane reforming. Modeling, simulation and optimization using a commercial process simulator are performed for the proposed hydrogen production process from glycerol. The mixture of glycerol and steam are used for making syngas in the reforming process. Then hydrogen are produced from carbon monoxide and steam through the water-gas shift reaction. Finally, hydrogen is separated from carbon dioxide using PSA. This study shows higher yield than former U.S. DOE and Linde studies. Economic evaluations are performed for optimal planning of constructing domestic hydrogen energy infrastructure based on the proposed glycerol-based hydrogen station.

Serovars of Xanthomonas campetris pv. oryzae Collected from Korea and Serological Diagnosis of Bacterial leaf Blight (우리나라 벼 흰빛잎마름병균 (Xanthomonas campestris pv. oryzae)의 혈청학적 분류 및 진단)

  • Choi J. E.;Lee D. K.;Seo J. H.
    • Korean Journal Plant Pathology
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    • v.1 no.1
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    • pp.61-66
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    • 1985
  • Seventy-one strains collected from Korea were classified into three serovars (designated A, B-I and B-II) by using agar gel diffusion test with the antisera produced against Xanthomonas campestris pv. oryzae isolates Q7472 and Q7502. Of 71 isolates tested, 65 isolates belonged to serovar A, 5 isolates were serovar B-I, and one isolate was serovar B-II. The isolates of serovar B-I and B-II could be distinguished clearly from those of serovar A showing marked autoagglutination. Xanthomonas campestris pv. oryzae was serologically diagnosed in rice leaves by agar gel diffusion tests, possibly being distinguished from Xanthomonas campestris pv. olyzicola and E. herbicola. The pathogen could be also serologically detected from the extracts of diseased leaves, squeezed immediately, heated at $100^{\circ}C$ or incubated in PSA. Serological detection of Xanthomonas campestris pv. oryzae is a more reliable and less time-comsuming method.

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A Study on Adhesion Performance of Styrene-Block-Copolymer Based Hot Melt Pressure Sensitive Adhesives with Dicyclopentadiene Based Hydrogenated Hydrocarbon Resins (수첨 DCPD계 석유수지를 이용한 SBCs계 핫멜트점착제의 접착성능 연구)

  • Shim, Jaeho;Kim, Yunho;Lee, Jungjoon
    • Journal of Adhesion and Interface
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    • v.15 no.4
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    • pp.145-150
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    • 2014
  • Dicyclopentadiene (DCPD)-based hydrocarbon resins are widely used as tackifiers in many applications. In particular, hydrogenated DCPD-based hydrocarbon resins are widely used in premium hot-melt-type adhesives such as hot melt adhesives (HMAs) and/or hot melt pressure-sensitive adhesives (HMPSAs), because are water-white in color and possess excellent stability to light and heat. This article discusses the adhesive performance of various hydrogenated DCPD resins when they are used as tackifiers in styrene-block-copolymer (SBC)-based HMPSAs. This article shows the correlation between the characteristics of tackifiers and the adhesive performance of SBC-based HMPSAs. The higher the softening point of the tackifier, the higher is the $T_g$, softening point, and crossover temperature of the PSAs. High aromatic H wt% content reduces the high-temperature resistance of PSAs, as suggested by the decrease in the crossover temperature and softening point of the PSAs.

Soil-to-Plant Transfer of $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ Deposited during the Growing Season of Potato (감자의 재배기간 중 토양에 침적한 $^{54}Mn,\;^{60}Co,\;^{85}Sr,\;^{137}Cs$의 작물체로의 전이)

  • Choi, Yong-Ho;Lim, Kwang-Muk;Jun, In;Keum, Dong-Kwon
    • Journal of Radiation Protection and Research
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    • v.33 no.3
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    • pp.105-112
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    • 2008
  • To measure the soil-to-plant transfer factors ($TF_a,\;m^2\;kg^{-1}$-fresh) of radionuclides deposited during the growing season of potato, a radioactive solution containing $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ was applied to the soil surfaces in soil boxes 2 d before seeding and three different times during the plant growth. For the pre-seeding application (PSA), radionuclides were mixed with the topsoil (loamy sand and 5.2 in pH). The plant parts investigated were leaves, stems, tuber skin and tuber flesh. The $TF_a$ values of $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ from the PSA were in the ranges of $1.9{\times}10^{-4}{\sim}1.5{\times}10^{-2}$, $1.8{\times}10^{-4}{\sim}7.5{\times}10^{-4}$, $4.0{\times}10^{-4}{\sim}1.6{\times}10^{-2}$, $1.5{\times}10^{-4}{\sim}3.9{\times}10^{-4}$ respectively, for different plant parts. The TFa values from the growing-time applications were on the whole a few times lower than those from the PSA. For $^{54}Mn,\;^{85}Sr$ and $^{137}Cs$, the $TF_a$ values from the early- or middle-growth-stage application were higher than those from the late-growth-stage application, whereas the opposite was true for $^{60}Co$. Leaves and tuber flesh had the highest and lowest $TF_a$ values, respectively, in most cases. The total uptake from soil by the four plant parts was in the range of $0.05{\sim}3.16%$. In the third year following the PSA, the $TF_a$ values of $^{54}Mn,\;^{60}Co$ and $^{137}Cs$ were $11{\sim}25%$, $21{\sim}25%$ and $38{\sim}67%$ of those in the first year, respectively, depending on the plant parts. The present results can be used for estimating the radiological impact of an acute radioactive deposition during the growing season of potato and for testing the validity of relevant food-chain models.

Verification of the upper limit of results through dilution tests for RIA test (RIA 검사별 희석실험을 통한 결과의 상한치 검증)

  • LEE, Geun Ui;CHOI, Jin Ju;LEE, Young Ji;YOO, Seon Hee;LEE, Sun Ho
    • The Korean Journal of Nuclear Medicine Technology
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    • v.26 no.1
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    • pp.42-46
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    • 2022
  • Purpose In the meantime, there have been not many samples that require dilution, and it has been difficult for the examiner to set an appropriate dilution multiple for RIA test item and report the results. Accordingly, it was judged that it was necessary to set the maximum dilution multiple for each test and to verify the upper limit of the clinical reportable range. Therefore, in this study, the maximum dilution multiple for each RIA test was set and the upper limit of the clinical reportable range was verified accordingly Materials and Methods Among all RIA tests conducted at Asan Medical Center, the study treated on 30 types of tests which also conduct the dilution test. Data from March to July 2021 were collected and analyzed. The study was conducted on samples subjected to serial dilutions such as X2, X4 or X10, X102, X103, X104, X105. Results Among a total of 30 test types, 18 test types have more than 5 N values in the tolerance range of 80~120%. As a result of the verification of maximum dilution multiples, the test set to 104 is 𝛼-fetoprotein and thyroglobulin, and the test set to 103 is CA-125, CEA, and 𝛽-hCG, and the test set to 102 is Free PSA, PSA, CA15-3, SCC, Ferritin, PTH, Cortisol, and Calcitonin. Tests set to 10 include three categories: 𝛽2-Microglobulin, C-peptide, and Testosterone. Conclusion It is expected that it will contribute to improving the quality of nuclear medicine blood tests as the results of dilution experiments can be reported quickly and accurately through the verification of the clinical reportable range.