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http://dx.doi.org/10.5333/KGFS.2002.22.1.051

Molecular Cloning and Characterization of a cDNA for the PSI-H Subunit Homolog of Photosystem I in Chinese Cabbage  

Cha, Joon-Yung (경상대학교 식물분자생물학 및 유전자조작연구소)
Choi, Young-Jin (경상대학교 식물분자생물학 및 유전자조작연구소)
Lee, Hyo-Shin (임업연구원 생물공학과)
Kim, Ki-Yong (축산기술연구소)
Park, Geun-Je (축산기술연구소)
Jo, Jin-Ki (경북대학교 동물공학과)
Son, Dae-Young (경상대학교 식물분자생물학 및 유전자조작연구소)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.22, no.1, 2002 , pp. 51-58 More about this Journal
Abstract
PSI-H is an intrinsic membrane protein known to be essential for efficient electron flow in PSI complex. We isolated a cDNA clone encoding a PSI-H subunit homolog from Chinese cabbage (Brassica campestris L.). The cDNA, designated bpsaH, had an insert of 435 bp and a full open reading frame that would encode a protein of 145 amino acids. The amino acid sequence deduced from the cDNA sequence is 79.3% identical to that of spinach, suggesting the cDNA most likely encodes Chinese cabbage PSI-H subunit. The bpasH was expressed at high level in leaf tissue and low level in flower bud, whereas it was undetectable in root tissue.
Keywords
Photosynthesis; Photosystem I; psaH; Brassica campestris;
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