Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Molecular Cloning and Characterization of a cDNA for the PSI-H Subunit Homolog of Photosystem I in Chinese Cabbage

배추로부터 광계 I의 PSI-H Subunit Homolog의 클로닝 및 분자생물학적 특성 연구

  • 차준영 (경상대학교 식물분자생물학 및 유전자조작연구소) ;
  • 최영진 (경상대학교 식물분자생물학 및 유전자조작연구소) ;
  • 이효신 (임업연구원 생물공학과) ;
  • 김기용 (축산기술연구소) ;
  • 박근제 (축산기술연구소) ;
  • 조진기 (경북대학교 동물공학과) ;
  • 손대영 (경상대학교 식물분자생물학 및 유전자조작연구소)
  • Published : 2002.03.01
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Abstract

PSI-H is an intrinsic membrane protein known to be essential for efficient electron flow in PSI complex. We isolated a cDNA clone encoding a PSI-H subunit homolog from Chinese cabbage (Brassica campestris L.). The cDNA, designated bpsaH, had an insert of 435 bp and a full open reading frame that would encode a protein of 145 amino acids. The amino acid sequence deduced from the cDNA sequence is 79.3% identical to that of spinach, suggesting the cDNA most likely encodes Chinese cabbage PSI-H subunit. The bpasH was expressed at high level in leaf tissue and low level in flower bud, whereas it was undetectable in root tissue.

식물의 광합성에 관여하는 광계 I의 protein subunit들의 연구는 최근까지도 극히 미약한 실정이며, 각각의 subunit들의 특성 또한 일부만이 밝혀져 있다. 본 연구진은 배추의 cDNA library로부터 식물에만 존재하는 subunit 중의 하나인, PSI-H subunit을 암호화하는 유전자인 bpsaH를 분리하였다. 이 유전자는 총 633 bp의 염기로 구성되어 있으며, 염기서열로부터 추정되는 분자량은 약 15,400이었고 등전점은 9.91이었다. 배추 PSI-H subunit의 아미노산 서열을 다른 식물체 유래의 단백질들과 비교분석한 결과, 시금치의 PSI-H와 가장 높은 유사성 (79.3%)을 나타내었다. 또한 bpsaH의 조직 특이적 발현 양상을 조사한 결과, 광합성 조직인 잎에서는 강하게 발현된 반면 꽃봉우리에서는 약하게 발현되었으며, 비광합성 조직인 뿌리에서는 전혀 발현되지 않았다.

Keywords

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