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http://dx.doi.org/10.7732/kjpr.2015.28.6.743

Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites  

Park, Hana (Department of Life Science, Gachon University)
Yoon, Chang Young (Department of Biotechnology, Shingyeong University)
Kim, Jin Sook (Korea Institute of Oriental Medicine)
Kim, Joo-Hwan (Department of Life Science, Gachon University)
Publication Information
Korean Journal of Plant Resources / v.28, no.6, 2015 , pp. 743-751 More about this Journal
Abstract
Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.
Keywords
Molecular identification; Reynoutria japonica; Reynoutria sachalinensis; SNP;
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