• Title/Summary/Keyword: progoitrin

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Identification and Quantitative Determination of Glucosinolates in Brassica napus cv. Hanakkori

  • Kim, Sun-Ju;Fujii, Kouei;Mohamed, Zaidul Islam Sarker;Kim, Hyun-Woong;Yamauchi, Hiroaki;Ishii, Gensho
    • Food Science and Biotechnology
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    • v.17 no.5
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    • pp.1097-1101
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    • 2008
  • The objective of this study was to identify and quantify glucosinolates (GSLs) in Brassica napus cv. Hanakkori and its parents and to evaluate its potential bitter taste. 'Hanakkori' materials were cultivated with commercial chemical nutrients (20 kg/ha, N-P-K: 16-10-10) at the field. GSLs were isolated by means of extraction with 70%(v/v) boiling methanol (MeOH) followed by desulfation from those plants by reversed-phase high performance liquid chromatography (HPLC) and identified by electronic spray ionization-mass spectrometry (ESI-MS) analysis. In 'Hanakkori', 11 GSLs were identified as progoitrin, glucoraphanin, glucoalyssin, gluconapoleiferin, gluconapin, 1-methylpropyl, glucobrassicanapin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin. The total GSL contents were 109 and 36.1 mmol/kg dry weights (d.w.) for the seeds and edible parts, respectively. The major GSLs (>5 mmol/kg d.w.) in the seeds were progoitrin (78.8), gluconapin (10.7), and glucobrassicanapin (7.81), whereas they in the edible parts were progoitrin (16.1) and glucobrassicanapin (8.58). In addition, the bitter taste in the edible parts was presumably related with the presence of progoitrin (>45% to the total GSL).

Determination of Goitrogenic Metabolites in the Serum of Male Wistar Rat Fed Structurally Different Glucosinolates

  • Choi, Eun-Ji;Zhang, Ping;Kwon, Hoonjeong
    • Toxicological Research
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    • v.30 no.2
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    • pp.109-116
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    • 2014
  • Glucosinolates (GLSs) are abundant in cruciferous vegetables and reported to have anti thyroidal effects. Four GLSs (sinigrin, progoitrin, glucoerucin, and glucotropaeolin) were administered orally to rats, and the breakdown products of GLSs (GLS-BPs: thiocyanate ions, cyanide ions, organic isothiocyanates, organic nitriles, and organic thiocyanates) were measured in serum. Thiocyanate ions were measured by colorimetric method, and cyanide ions were measured with CI-GC-MS. Organic isothiocyanates and their metabolites were measured with the cyclocondensation assay. Organic nitriles and organic thiocyanates were measured with EI-GC-MS. In all treatment groups except for progoitrin, thiocyanate ions were the highest among the five GLS-BPs. In the progoitrin treated group, a high concentration of organic isothiocyanates (goitrin) was detected. In the glucoerucin treated group, a relatively low amount of goitrogenic substances was observed. The metabolism to thiocyanate ions happened within five hours of the administration, and the distribution of GLSs varied with the side chain. GLSs with side chains that can form stable carbocation seemed to facilitate the degradation reaction and produce a large amount of goitrogenic thiocyanate ions. Because goitrogenic metabolites can be formed without myrosinase, the inactivation of myrosinase during cooking would have no effect on the anti-nutritional effect of GLSs in cruciferous vegetables.

Variation of Glucosinolate Contents among Domestic Broccoli (Brassica oleracea L. var. italica) Accessions (국내 브로콜리(Brassica oleracea L. var. italica) 유전자원 내 Glucosinolate 함량 변이)

  • Lee, Jun Gu;Kwak, Jung-Ho;Um, Yeong Cheol;Lee, Sang Gyu;Jang, Yoon-Ah;Choi, Chang Sun
    • Horticultural Science & Technology
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    • v.30 no.6
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    • pp.743-750
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    • 2012
  • A total of 95 broccoli (Brassica oleracea L. var. italica) accessions were evaluated for the identification of desulfo-glucosinolates and their content variation in the flower head using ultra performance liquid chromatography, to select the potentially functional broccoli breeding lines. The six individual desulfo-glucosinolates, including progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicanapin, and glucobrassicin, were commonly identified, based on the chromatogram peak comparison with those of the nine individual glucosinolate standards. The total glucosinolate contents varied from 4.2 to $29.0{\mu}mol{\cdot}g^{-1}$ DW and the glucoraphanin (1.6 to $13.9{\mu}mol{\cdot}g^{-1}$ DW) was confirmed as a major constituent in the total glucosinolate profile among the six identified individual glucosinolate species, whereas the progoitrin, which was only detected in 13 accessions, showed accession-specific variation and negative correlation with glucoraphanin content. It was also revealed that the four major glucosinolates, such as glucobrassicanapin, glucoraphanin, glucobrassicin, and gluconapin, affected major content variation and showed higher positive inter-correlation. These results might be used for the selection of potential breeding materials as functional broccoli germplasm through the further evaluation on the stability and reproducibility of glucosinolate profile depending on environmental factors or cultural managements using the selected accessions.

Variation of Glucosinolate Composition during Seedling and Growth Stages of Brassica rapa L. ssp. pekinensis

  • Hong, Eunyoung;Kim, Gun-Hee
    • Horticultural Science & Technology
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    • v.32 no.5
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    • pp.730-738
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    • 2014
  • The objective of this study was to evaluate glucosinolate (GSL) profiles and variation of total and individual GSLs concentrations within seedling (0-14 days) and growth stages (0-15 weeks) of Korean Chinese cabbage (Brassica rapa L. ssp. pekinensis). Ten GSLs (progoitrin, glucoraphanin, glucoalyssin, gluconapin, glucobrassicanapin, 4-hydroxyglucobrassin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and gluconasturtiin) were identified from Korean Chinese cabbage. In general, total GSL content significantly decreased during seedling (from 92.89 to $35.26{\mu}mol{\cdot}g^{-1}$ DW) and g rowth stages ( from 74.11 to $1.97{\mu}mol{\cdot}g^{-1}$ DW). Gluconapin was the highest in seeds and in the germination period ($73.1{\mu}mol{\cdot}g^{-1}$ DW) and declined gradually from 73 to 15% during seedling stages. The level of the major aliphatic GSLs, gluconapin and progoitrin, tended to decrease sharply, whereas levels of indolic GSLs (4-methoxyglucobrassicin, glucobrassicin) and aromatic GSLs (gluconasturtiin) were found to increase generally at the beginning of growth stages.

Variations of glucosinolates in kale leaves (Brassica oleracea var. acephala) treated with drought-stress in autumn and spring seasons (수분스트레스에 의한 케일 내 글루코시놀레이트 변화)

  • Jeong, Na-Rae;Chun, Jin-Hyuk;Park, Eun-Jae;Lim, Ye-Hoon;Kim, Sun-Ju
    • Korean Journal of Agricultural Science
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    • v.42 no.3
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    • pp.167-175
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    • 2015
  • The present study aimed to investigate the effects of drought stress on the accumulation of glucosinolates (GSLs) in the leaves of Kale cultivated in autumn and spring. HPLC analysis guided to identify seven GSLs including progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicin, 4-methoxyglucobrassicin and neoglucobrasscin. Quantification of GSLs revealed that the contents of sigirin was the highest (45%) followed by the level of progoitrin (24%) in terms of total GSLs. The ranges of total GSL contents was 1.16 (84)-15.88 (89 DAS, ${\mu}mol/g$ dry wt. (DW)) in treatment plot and 1.23 (84)-7.05 (74 DAS, ${\mu}mol/g$ dry wt.) in control plot showed the enhancement in the contents of GSLs in treatment than in the control plot. The present results evidenced that the variation of total GSL contents were depending on the harvest period. In 105 DAS, comparatively no differences in the GSL contents on each sample in autumn season, whereas in spring season, although there was decrease in the GSLs tendency from 74 DAS to 84 DAS in both control and treatment plot, the GSL contents of treatment plot was dramatically increased in 89 DAS. In treatment plot, the GSL contents on 89 DAS (1.16) was 15 fold higher to 84 DAS ($15.88{\mu}mol/g$ DW). The variation in the contents of GSL in spring and autumn did not documented significant differences because of their differences in the growth time and cultivation conditions. In conclusion, the GSL contents in kale was likely to be affected by drought stress treatment. Scrutiny and further research for exact relation between drought stress and GSL contents in kale should be needed.

Effects of different sulfur ion concentration in nutrient solution and light source on glucosinolate contents in kale sprouts (Brassica oleracea var. acephala)

  • Park, Ye-Jin;Chun, Jin-Hyuk;Woo, Hyunnyung;Maruyama-Nakashita, Akiko;Kim, Sun-Ju
    • Korean Journal of Agricultural Science
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    • v.44 no.2
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    • pp.261-271
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    • 2017
  • The aim of this study was to investigate the amount of glucosinolates (GSLs) in kale sprouts (Brassica oleracea L. var. acephala) ('TBC') according to different concentrations of sulfur ions in sprout's nutrient solutions (0.0, 0.5, 1.0, and 2.0 mM) and to different light sources [Fluorescent lamp, Red, Blue, and Mix (R+B) LED]. Kale sprouts were cultivated in a growth chamber for 13 days in sulfur solutions. Kale sprouts were treated with fluorescent lamp and LED light sources for 5 days, from eight days after sowing to harvest. Amount of seven types of GSLs (progoitrin, sinigrin, 4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin) were measured in kale sprouts after harvest. The total GSL content was influenced by different sulfur solution concentration, and it was the highest at S 0.5 mM ($172.54{\mu}mol{\cdot}g^{-1}DW$) and the lowest at S 2.0 mM ($163.09{\mu}mol{\cdot}g^{-1}DW$). The GSL content was influenced by different light source, and it was the highest with Red LED ($159.23{\mu}mol{\cdot}g^{-1}DW$) and the lowest with Blue LED ($147.57{\mu}mol{\cdot}g^{-1}DW$). As the sulfur solution concentration increased under all light source, progoitrin and sinigrin contents tended to decrease while glucobrassicin content showed an upward tendency for all of the light sources. The content of glucobrassicin was higher than that of progitrin when treated with sulfur solutions for all LED light sources. Sinigrin, which has excellent anti-cancer effects, showed the highest rate (92.2%) among all the GSLs, under all of the light sources.

The Effect Control of Root-knot Nematode by Using Rapeseed Meal in Continuous Cultivation at Greenhouse (유채박 이용 시설하우스 연작재배 시 뿌리혹선충 밀도억제효과)

  • Lee, Hoo-Kwan;Lee, Young-Hwa;Kim, Kwang-Soo;Jang, Young-Seok;Choi, In-Hu
    • Korean Journal of Plant Resources
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    • v.28 no.1
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    • pp.93-100
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    • 2015
  • The objective of this study was to determine the effectiveness of rapeseed meal in controlling soil nematode. Two different rapeseed meals (Jeju local rape varieties and 'Sunmang' variety) were mixed with the soil to control nematodes environmentally. When soil physical properties in the rapeseed meal-mixed soils were analyzed, OM (organic matter), $P_2O_5$, Ca, Mg, CEC (Cation Exchange Capacity) value increased. Especially, the level of OM was 3-fold higher than control soil. Glucosinolate content of rapeseed meal was higher in Jeju local rape varieties than 'Sunmang' variety. The major components of glucosinolates were consisted of progoitrin, gluconapin, glucobrassiaca napin, and sinigrin. These components were likely to be involved in reducing nematode density.

Effect of Developmental Stages on Glucosinolate Contents in Kale (Brassica oleracea var. acephala) (생장단계에 따른 케일 내 글루코시놀레이트 함량)

  • Lee, Heon-Hak;Yang, Si-Chang;Lee, Min-Ki;Ryu, Dong-Ki;Park, Suhyoung;Chung, Sun-Ok;Park, Sang Un;Lim, Yong-Pyo;Kim, Sun-Ju
    • Horticultural Science & Technology
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    • v.33 no.2
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    • pp.177-185
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    • 2015
  • The aim of this study was to investigate the amounts of glucosinolates (GSL) in kale at various development stages. Kale varieties 'Manchoo Collard' and 'TBC' were cultivated from 20 February 2012 to 3 July 2013 in the greenhouse at Chungnam National University. During the cultivation periods, samples were harvested at 35, 63, 91, 105, 119, and 133 days after sowing (DAS) and the amount of GSL quantified by HPLC. Ten types of GSL (progoitrin, sinigrin, glucoalyssin, gluconapin, glucoiberverin, 4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin) were observed in 'TBC', whereas nine types of GSL (the same as above, except glucoiberverin) were identified in 'Manchoo Collard'. The amount of total GSL in 'Manchoo Collard' was comparatively higher at 133 DAS (mean $8.64{\mu}mol{\cdot}g^{-1}$) and lower at 35 DAS ($1.16{\mu}mol{\cdot}g^{-1}$ dry weight, DW) of cultivation. In the case of 'TBC', the amount of GSL was higher at 91 DAS (mean $13.41{\mu}mol{\cdot}g^{-1}$) and lower at 35 DAS ($0.31{\mu}mol{\cdot}g^{-1}$ dry weight, DW). Sinigrin was the most abundant GSL (57% of total GSL) in 'Manchoo Collard' at 133 DAS and was also highest (44%) in 'TBC' at 91 DAS. Together, progoitrin, sinigrin, glucobrassicin, and gluconasturtiin, the precursor of crambene, allylisothiocyanate, indol-3-cabinol, and phenethylisothiocyanate accounted for 94 and 78% of GSL in 'Manchoo Collard' and 'TBC', respectively. Our results demonstrate that the amounts of GSL, which have potential anti-carcinogenic activity, change during development in kale.

Identification and Quantification of Glucosinolates in Rapeseed (Brassica napus L.) Sprouts Cultivated under Dark and Light Conditions

  • Lee, Min-Ki;Arasu, Mariadhas Valan;Chun, Jin-Hyuk;Seo, Jeong Min;Lee, Ki-Teak;Hong, Soon-Taek;Kim, In Ho;Lee, Yong-Hwa;Jang, Young-Seok;Kim, Sun-Ju
    • Korean Journal of Environmental Agriculture
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    • v.32 no.4
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    • pp.315-322
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    • 2013
  • BACKGROUND: This study was performed for the identification and quantification of glucosinolate (GSL) contents in seven varieties of rapeseed (Brassica napus L.) sprouts cultivated under dark and light conditions. METHODS AND RESULTS: Crude glucosinolates (GSLs) were desulfated by treating with aryl sulfatase and purified using diethylaminoethyl sepharose (DEAE) anion exchange column. Individual GSLs were quantified using high-performance liquid chromatography (HPLC) with electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Eleven GSLs including six aliphatic (progoitrin, sinigrin, glucoalyssin, gluconapoleiferin, gluconapin, and glucobrassicanapin), four indolyl (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin) and one aromatic (gluconasturtiin) were identified based on the fragmentation patterns of MS spectrum. Aliphatic GSLs were noted as the predominant group with average 85.2% of the total contents. The most abundant GSLs were progoitrin which was ranged at $8.14-118.68{\mu}mol/g$ dry weight (DW). The highest total GSL amounts were documented in 'Hanra' ($146.02{\mu}mol/g$ DW) under light condition and 'Mokpo No. 68' ($86.67{\mu}mol/g$ DW) in dark condition, whereas the lowest was in 'Tamra' (30.13 and $14.50{\mu}mol/g$ DW) in both conditions. The sum of aliphatic GSLs attributed > 80% in all varieties, except 'Tamra' (67.7% and 64.9% in dark and light conditions, respectively) in the total GSL accumulation. Indolyl GSLs were ranged $2.41-15.73{\mu}mol/g$ DW, accounted 2.78-33.6% of the total GSLs in rapeseed varieties. CONCLUSION(S): These results provide valuable information regarding potential beneficial GSL contents individually. This study attempts to contribute to knowledge of the nutritional properties of the different varieties of rapeseed plants. These results may be useful for the evaluation of dietary information.

Effects of Artificial Light Sources on Growth and Glucosinolate Contents of Hydroponically Grown Kale in Plant Factory (식물공장 인공광원이 케일의 생육 및 글루코시놀레이트 함량에 미치는 영향)

  • Lee, Guang-Jae;Heo, Jeong-Wook;Jung, Chung-Ryul;Kim, Hyun-Hwan;Jo, Jung-Su;Lee, Jun-Gu;Lee, Gyeong-Ja;Nam, Sang-Young;Hong, Eui-Yon
    • Journal of Bio-Environment Control
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    • v.25 no.2
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    • pp.77-82
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    • 2016
  • This study was carried out to investigate the effects of artificial light sources on growth, yield, and glucosinolate content of hydoroponically grown Peucedanum japonicum in plant factory. Treatments were given with LED Blue:White(1:1, B:W), LED Red:Blue:White(2:1:3, RBW), and LED Blue:White(1:1)+Florescent lamp(BW+FL). Number of harvested leaves and leaf weight of BW+FL were higher than BW and RBW. BW+FL in leaf length and RBW in leaf width were significant difference with other treatments. Chlorophyll content and 'L' value were not significant difference among the treatments. The 'a' and 'b' value is the lowest in BW+FL. Glucosinolate content was high in order of glucobrassicin, glucoiberin, sinigrin, gluconasturtiin, progoitrin, glucoraphamin, and epiprogoitrin in all treatments, and total glucosinolate content was the highest in RBW treatment. Moisture, crude protein, crude fat, and ash content of leaves were not different among the treatments. In conclusion, this study showed that light caused growth and secondary metabolites synthesis, and we recommend to further study between light and secondary metabolites for increasing functionality.