Browse > Article

Identification and Quantitative Determination of Glucosinolates in Brassica napus cv. Hanakkori  

Kim, Sun-Ju (Crop Functionality and Utilization Research Subteam, Upland Farming Research Station, National Agriculture Research Center for Hokkaido Region)
Fujii, Kouei (Department of Crop Breeding, Yamaguchi Prefecture Agricultural Research Institute)
Mohamed, Zaidul Islam Sarker (Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia)
Kim, Hyun-Woong (Life and Environmental Sciences Design, Faculty of Engineering, Chiba Institute of Technology)
Yamauchi, Hiroaki (Crop Functionality and Utilization Research Subteam, Upland Farming Research Station, National Agriculture Research Center for Hokkaido Region)
Ishii, Gensho (Local Crop Breeding Research Team, National Agricultural Research Center for Hokkaido Region)
Publication Information
Food Science and Biotechnology / v.17, no.5, 2008 , pp. 1097-1101 More about this Journal
Abstract
The objective of this study was to identify and quantify glucosinolates (GSLs) in Brassica napus cv. Hanakkori and its parents and to evaluate its potential bitter taste. 'Hanakkori' materials were cultivated with commercial chemical nutrients (20 kg/ha, N-P-K: 16-10-10) at the field. GSLs were isolated by means of extraction with 70%(v/v) boiling methanol (MeOH) followed by desulfation from those plants by reversed-phase high performance liquid chromatography (HPLC) and identified by electronic spray ionization-mass spectrometry (ESI-MS) analysis. In 'Hanakkori', 11 GSLs were identified as progoitrin, glucoraphanin, glucoalyssin, gluconapoleiferin, gluconapin, 1-methylpropyl, glucobrassicanapin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin. The total GSL contents were 109 and 36.1 mmol/kg dry weights (d.w.) for the seeds and edible parts, respectively. The major GSLs (>5 mmol/kg d.w.) in the seeds were progoitrin (78.8), gluconapin (10.7), and glucobrassicanapin (7.81), whereas they in the edible parts were progoitrin (16.1) and glucobrassicanapin (8.58). In addition, the bitter taste in the edible parts was presumably related with the presence of progoitrin (>45% to the total GSL).
Keywords
bitterness; Brassica crop; glucosinolate; interspecific hybridization; progoitrin;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Fahey JW, Zalcmann AT, Talalay P. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56: 5-51 (2001)   DOI   ScienceOn
2 Katakawa S. The characterization and cultivation of a new Brassica vegetable 'Hanakkori'. pp. 70-75. Agriculture of This Month: The Chemical Daily, Tokyo, Japan (1997)
3 Gland A, Robbelen G, Thies W. Variation of alkenyl glucosinolates in seeds of Brassica species. Z. Pflanzenzuchtg. 87: 96-110 (1981)
4 Kim S-J, Kawaharada C, Jin S, Hashimoto M, Ishii G, Yamauchi H. Structural elucidation of 4-(cystein-S-yl)butyl glucosinolate from the leaves of Eruca sativa. Biosci. Biotech. Bioch. 71: 114-121 (2007)   DOI   ScienceOn
5 Kim S-J, Ishii G. Effect of storage temperature and duration on glucosinolate, total vitamin C, and nitrate contents in rocket salad (Eruca sativa Mill.). J. Sci. Food Agr. 87: 966-973 (2007)   DOI   ScienceOn
6 European Committee. Commission Regulation (EC) 2316/1999 of 22 October 1999 laying down detailedrules for the application of Council Regulation (EC) No 1251/1999 establishing asupport system for producers of certain arable crops. OJ L 280, 30.10.1999, pp. 43-65. Available from: http://europa.eu.int/smartapi/cgi/sga_doc?smartapi!celexapi!prod!CELEXnumdoc&lg=EN&numdoc=31999R2316&model=guichett. Accessed May 7, 2007
7 Van Doorn HE, van der Kruk GC, van Holst G-J, Raaijmakers-Ruijs NCME, Postma E, Groeneweg B, Jongen WHF. The glucosinolates sinigrin and progoitrin are important determinants for taste preference and bitterness of Brussels sprouts. J. Sci. Food Agr. 78: 30-38 (1998)   DOI   ScienceOn
8 Bell JM. Nutrients and toxicants in rapeseed meal: A review. J. Anim. Sci. 58: 996-1010 (1984)   DOI
9 Fenwick GR, Griffiths NM. The identification of the goitrongen, (-) 5-vinyloxazolidine-2-thione (goitrin), as a bitter principle of cooked Brussels sprouts (Brassica oleracea L. var. gemmifera). Z. Lebensm. Unters. For. 172: 90-92 (1981)   DOI
10 Tompson KF. Breeding winter oilseed rape, Brassica napus. Adv. Appl. Biol. 7: 1-104 (1983)
11 Magrath R, Bano F, Morgner M, Parkin I, Sharpe A, Lister C, Dean C, Turner J, Lydiate D, Mithen R. Genetics of aliphatic glucosinolates (I). Side chain elongation in Brassica napus and Arabidopsis thaliana. Heredity 72: 290-299 (1994)   DOI   ScienceOn
12 Kim S-J, Kawaguchi S, Watanabe Y. Glucosinolate in vegetative tissues and seeds of twelve cultivars of vegetable turnip rape (Brassica rapa L.). Soil Sci. Plant Nutr. 49: 337-346 (2003)   DOI   ScienceOn
13 Yamamoto Y, Kaneko K, Okafuji Y. Plant regeneration from in vitro cultured plotoplasts and anthers of 'Hanaccori' (Brassica rapa L. x B. oleracea L.) and chracters in the progency lines derived from regenerated plants (in Japanese with English summary). Bull. Yamaguchi Agric. Expt. Sta. 53: 35-40 (2002)
14 Fenwick GR, Griffiths NM, Hanley AB. Bitterness in Brussels sprouts (Brassica oleracea L. var. gemmifera): The role of glucosinolates and their breakdown products. J. Sci. Food Agr. 34: 73-80 (1983)   DOI
15 Reichelt M, Brown PD, Schneider B, Oldham NJ, Stauber E, Tokuhisa J, Kliebenstein DJ, Mitchell-Olds T, Gershenzon J. Benzoic acid glucosinolate esters and other glucosinolates from Arabidopsis thaliana. Phytochemistry 59: 663-671 (2002)   DOI   ScienceOn
16 Fenwick GR, Heaney RK, Mullin WJ. Glucosinolates and their breakdown products in food and food plants. Crit. Rev. Food Sci. 18: 123-201 (1983)   DOI   ScienceOn
17 Chisholm MD, Wetter LR. The biosynthesis of some isothiocyanates and oxazolidinethiones in rape (Brassica campestris L.). Plant Physiol. 42: 1726-1730 (1967)   DOI   ScienceOn
18 Matsumoto O, Okafuji Y, Kaneko K, Katakawa S. Breeding of new vegetable 'Hanakkori' by ovule culture. Bull. Yamaguchi Agric. Expt. Sta. 48: 21-24 (1997)
19 Sones K, Heaney RK, Fenwick GR. An estimate of the mean daily intake of glucosinolates from cruciferous vegetables in the UK. J. Sci. Food Agr. 35: 712-720 (1984)   DOI
20 Kim S-J, Ishii G. Glucosinolate profiles in the seeds, leaves, and roots of rocket salad (Eruca sativa Mill.) and antioxidative activities of intact plant powder and purified 4-methoxyglucobrassicin. Soil Sci. Plant Nutr. 52: 394-400 (2006)   DOI   ScienceOn
21 Chen S, Petersen BL, Olsen CE, Schulz A, Halkier BN. Longdistance phloem transport of glucosinolates in arabidopsis. Plant Physiol. 127: 194-201 (2001)   DOI   ScienceOn
22 Parkin I, Magrath R, Keith D, Sharpe A, Mithen R, Lydiate D. Genetics of aliphatic glucosinolates. II. Hydroxylation of alkenyl glucosinolates in Brassica napus. Heredity 72: 594-598 (1994)   DOI   ScienceOn
23 Sang JP, Minchinton IR, Johnstone PK, Truscott RJW. Glucosinolate profiles in the seed, root, and leaf tissue of cabbage, mustard, rapeseed, radish, and swede. Can. J. Plant Sci. 64: 77-93 (1984)   DOI
24 Halkier BA, Du L. The biosynthesis of glucosinolates: Reviews. Trends Plant Sci. 2: 425-431 (1997)   DOI   ScienceOn
25 ISO 9167-1 2, 1-9. Rapeseed-determination of glucosinolates content. The International Organization for Standardization, Geneva, Switzwerland (1992)
26 Tookey HL, Daxenbichler ME, VanEtten CH. Cabbage glucosinolates: Correspondence of patterns in seeds and leafy heads. J. Am. Soc. Hortic. Sci. 105: 714-717 (1980)
27 Carlson DG, Daxenbichler ME, Tookey HL. Glucosinolates in turnip tops and roots: Cultivars grown for greens and/or roots. J. Am. Soc. Hortic. Sci. 112: 179-183 (1987)