• Proceedings of the Korean Society of Precision Engineering Conference
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• 2013.05a
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• pp.181-182
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• 2013

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2011.06a
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• pp.1459-1460
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• 2011

• Journal of the Korean Society for Precision Engineering
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• v.29 no.1
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• pp.33-40
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• 2012

The scaffold serves as 3D substrate for the cells adhesion and mechanical support for the newly grown tissue by maintaining the 3D structure for the regeneration of tissue and organ. In this paper, we proposed integrated scaffold fabrication system using multi-axis rapid prototyping (RP) technology. It can fabricate various types of scaffolds: arbitrary sculptured shape, primitive shape, and tube shape scaffolds by layered dispensing biocompatible/ biodegradable polymer strands in designated patterns. In order to fabricate the 3D scaffold, we need to generate the plotting path way for the scaffold fabrication system. We design a data processing program - scaffold plotting software, which can convert the 3D STL file, primitive and tube model images into the NC code for the system. Finally, we fabricated the customized 3D scaffolds with high accuracy using the plotting software and the fabrication system.

• Polymer(Korea)
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• v.32 no.6
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• pp.516-522
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• 2008

Biodegradable polymers have been used extensively as scaffolding materials to regenerate new tissues and the ingrowth of tissue have been reported to be dependent directly of the porosity, pore diameter, pore shape, and porous structure of the scaffold. In this study, porous poly (L-lactide-co-glycolide) (PLGA) scaffolds with five different pore sizes were fabricated to investigate the effect of pore sizes for AF tissue regeneration. Cellular viability and proliferation were assayed by MTT test. Hydroxyproline/DNA content of AF cells on each scaffold was measured. sGAG analyses were performed at each time point of 2 and 6 weeks. Scaffold seeded AF cells were implanted into the back of athymic nude mouse to observe the difference of formation of disc-like tissue depending on pore size in vivo. We confirmed that scaffold with $180{\sim}250{\mu}m$ pores displayed high cell viability in vitro and produced higher ECM than scaffold with other pore sizes in vivo.

• Polymer(Korea)
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• v.38 no.4
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• pp.449-456
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• 2014

Ternary blends of poly(L-lactic acid) (PLLA), poly(${\varepsilon}$-caprolactone) (PCL) and polyethylene oxide (PEO) were produced with different concentrations of components via melt blending. By leaching the PEO from the samples by water, porous materials were obtained with potential application for bio scaffolds. Sample porosity was evaluated by calculating the ratio of porous scaffold density (${\rho}^*$) to the non-porous material density (${\rho}_s$). Highest porosity (51.42%) was related to the samples containing 50 wt%. of PEO. Scanning electron microscopy (SEM) studies showed the best porosity resulted by decreasing PLLA/PCL ratio at constant concentration of PEO. Crystallization behavior of the ternary blend samples was studied using differential scanning calorimetry (DSC). Results revealed that the crystallinity of PLLA was improved by addition of PEO and PCL to the samples. The porosity plays a key role in governing the compression properties. Mechanical properties are presented by Gibson-Ashby model.

• Journal of the Korean Chemical Society
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• v.50 no.3
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• pp.224-231
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• 2006

agents used for enhancing mechanical properties of porous natural scaffolds, reduces biocompatability of the scaffolds, due to their inherent cytotoxicity. In this study, scaffolds which was composed of chitosan, alginate and gelatin were cross-linked by using heat treatment instead of cross-linking agent and mechanical properties of the cross-linked scaffold were investigated. Fourier transform infrared spectroscopy (FT-IR) analysis confirmed that cross-linking of heat-treated scaffold was formed via amide or ester linkage between the polymer chains. The heat-treated scaffold had interconnected pores with mean diameter of 100~200 m and showed more than two fold increase of water uptake in comparison with chemically cross-linked scaffold. Tensile strength of the heat-treated scaffold increased up to 130% compared to non cross-linked scaffold and average maximum elongation was 11.3%. The porous cross-linked scaffold with the improved mechanical property may be suitable as a biocompatable scaffold for tissue engineering.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2012.05a
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• pp.1141-1142
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• 2012

• Journal of the Korean Society for Precision Engineering
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• v.33 no.1
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• pp.69-75
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• 2016

Polydimethylsiloxane (PDMS) is used as a scaffold for cell culture. Because both the stress and strain acting on the substrate and the hemodynamic environment are important for studying mechano-transduction of cellular function, the traction force of the surface of a substrate has been measured using fluorescence images of particle distribution. In this study, deformation of the cross-sectional plane of a PDMS block was measured by correlating particle image distributions to validate the particle image strain measurement technique. Deformation was induced by a cone indentor and a shearing parallel plate. Measured deformations from particle image distributions were in agreement with the results of a computational structure analysis using the finite-element method. This study demonstrates that the particle image correlation method facilitates measurement of deformation of a polymer scaffold in the cross-sectional plane.

• Polymer(Korea)
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• v.35 no.5
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• pp.378-384
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• 2011

Silk fibroin is a biocompatible and slowly biodegradable natural polymer. This natural polymer has excellent mechanical properties, non-toxicity, and non-immunogenic properties and has been demonstrated to support tissue regeneration. Also, gelatin is a natural material derived from collagen by hydrolysis and has an almost identical composition as that of collagen. Silk fibroin/gelatin scaffolds have been fabricated by using the freeze-drying method. To establish the scaffold manufacturing condition for silk fibroin and gelatin, we made scaffolds with various compositions of gelatin, glutaldehyde and silk fibroin. The silk fibroin/gelatin scaffolds were characterized using SEM, DSC, and water absorption ability tests. The cellular proliferation was evaluated by WST assay. These results suggested that a scaffold containing 8% of gelatin, 1% of glutaldehyde and 0.3 g of silk fibroin provided suitable characterstics for cell adhesion and proliferation. In conclusion, the silk fibroin/gelatin scaffold may serve as a potential cell delivery vehicle and a structural basis for tissue engineering.

• Polymer(Korea)
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• v.34 no.5
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• pp.398-404
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• 2010

We fabricated sponge and poly(lactide-co-glycolide)(PLGA) scaffolds impregnated demineralized bone particle(DBP)(DBP/PLGA) and investigated proper condition to proliferation and phenotype maintenance of intervertebral disc(IVD) cells by comparison between DBP/PLGA scaffold and DBP sponge. DBP/PLGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in scaffolds of two types. Cell viability and proliferation according to scaffolds were analyzed by WST assay and SEM. RT-PCR was assessed to measure mRNA expression of aggrecan and type II collagen of human IVD cells. In WST assay results, cell viability in scaffolds impregnated DBP/PLGA scaffold were higher than DBP sponge. We could observe that disc cell mRNA expressed better in DBP/PLGA scaffold than DBP sponge. We concluded that the using of DBP/PLGA in terms of scaffold fabrication for bio-disc with human IVD cells is helpful growth of disc cells maintenance of phenotypes.