Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
권호 표지

The Comparison of Sponges and PLGA Scaffolds Impregnated with DBP on Growth Behaviors of Human Intervertebral Disc Cells

DBP 스폰지와 DBP/PLGA 지지체에서의 인간 디스크세포 거동분석 비교

  • Lee, Seon-Kyoung (Department of BIN Fusion Technology & Department of Polymer.Nano Science & Technology, Chonbuk National University) ;
  • Hong, Hee-Kyung (Department of BIN Fusion Technology & Department of Polymer.Nano Science & Technology, Chonbuk National University) ;
  • Kim, Su-Jin (Department of BIN Fusion Technology & Department of Polymer.Nano Science & Technology, Chonbuk National University) ;
  • Kim, Yong-Ki (Department of BIN Fusion Technology & Department of Polymer.Nano Science & Technology, Chonbuk National University) ;
  • Song, Yi-Seul (Department of BIN Fusion Technology & Department of Polymer.Nano Science & Technology, Chonbuk National University) ;
  • Ha, Yoon (Department of Neurosurgery, Spine & Spinal Cord Institute, College of Medicine, Yonsei University) ;
  • Lee, Dong-Won (Department of BIN Fusion Technology & Department of Polymer.Nano Science & Technology, Chonbuk National University) ;
  • Khang, Gil-Son (Department of BIN Fusion Technology & Department of Polymer.Nano Science & Technology, Chonbuk National University)
  • 이선경 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 홍희경 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 김수진 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 김용기 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 송이슬 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 하윤 (연세대학교 의과대학 신경외과) ;
  • 이동원 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 강길선 (전북대학교 BIN 융합공학과, 고분자 나노공학과)
  • Received : 2010.01.14
  • Accepted : 2010.05.03
  • Published : 2010.09.25
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Abstract

We fabricated sponge and poly(lactide-co-glycolide)(PLGA) scaffolds impregnated demineralized bone particle(DBP)(DBP/PLGA) and investigated proper condition to proliferation and phenotype maintenance of intervertebral disc(IVD) cells by comparison between DBP/PLGA scaffold and DBP sponge. DBP/PLGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in scaffolds of two types. Cell viability and proliferation according to scaffolds were analyzed by WST assay and SEM. RT-PCR was assessed to measure mRNA expression of aggrecan and type II collagen of human IVD cells. In WST assay results, cell viability in scaffolds impregnated DBP/PLGA scaffold were higher than DBP sponge. We could observe that disc cell mRNA expressed better in DBP/PLGA scaffold than DBP sponge. We concluded that the using of DBP/PLGA in terms of scaffold fabrication for bio-disc with human IVD cells is helpful growth of disc cells maintenance of phenotypes.

본 연구팀은 DBP를 함침시킨 물성이 서로 다른 스폰지와 PLGA 지지체를 제작한 후 세포 부착, 증식 및 형태 유지를 알아보기 위한 실험을 수행하였다. WST 분석법과 SEM 관찰을 통하여 스폰지에 비해서 PLGA 지지체에서의 세포의 증식이 활발한 것을 확인하였고, RT-PCR을 통해 디스크세포에서 특이적으로 발현하는 제 2형 콜라겐과 어그리칸의 발현을 확인하였다. WST 결과, 세포 증식률은 DBP/PLGA 지지체가 DBP를 함침시킨 스폰지보다 세포 증식률이 높음을 확인하였다. 본 연구팀은 스폰지보다 PLGA 지지체가 인간디스크의 표현형 유지 및 증식에 있어서 긍정적인 영향을 미치는 것을 확인하였다.

Keywords

References

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