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Application of Montmorillonite as Capping Material for Blocking of Phosphate Release from Contaminated Marine Sediment (해양오염퇴적물 내 인산염 용출차단을 위한 피복소재로서의 몬모릴로나이트 적용)

  • Kang, Ku;Kim, Young-Kee;Hong, Seong-Gu;Kim, Han-Joong;Park, Seong-Jik
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.8
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    • pp.554-560
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    • 2014
  • To investigate the applicability of montmorillonite to capping material for the remediation of contaminated marine sediment, adsorption characteristics of $PO{_4}{^{3-}}$ onto montmorillonite were studied in a batch system with respect to changes in contact time, initial concentration, pH, adsorbent dose amount, competing anions, adsorbent mixture, and seawater. Sorption equilibrium reached in 1 h at 50 mg/L but 3 h was required to reach sorption equilibrium at 300 mg/L. Freundlich model was more suitable to describe equilibrium sorption data than Langmuir model. The $PO{_4}{^{3-}}$ adsorption decreased as pH increased, due to the $PO{_4}{^{3-}}$ competition for favorable adsorption site with OH- at higher pH. The presence of anions such as nitrate, sulfate, and bicarbonate had no significant effect on the $PO{_4}{^{3-}}$ adsorption onto the montmorillonite. The use of the montmorillonite alone was more effective for the removal of the $PO{_4}{^{3-}}$ than mixing the montmorillonite with red mud and steel slag. The $PO{_4}{^{3-}}$ adsorption capacity of the montmorillonite was higher in seawater than deionized water, resulting from the presence of calcium ion in seawater. The water tank elution experiments showed that montmorillonite capping blocked well the elution of $PO{_4}{^{3-}}$, which was not measured up to 14 days. It was concluded that the montmirillonite has a potential capping material for the removal of the $PO{_4}{^{3-}}$ from the aqueous solutions.

Analysis of adsorption behavior of lead ion on to surface modified AlPO4 materials (표면처리된 AlPO4에 대한 납 이온의 흡착 거동 분석)

  • Kim, Young-Ho;Kil, Hyun-Suk;Kang, Kwang-Cheol;Choi, Suk-Nam;Rhee, Seog-Woo
    • Analytical Science and Technology
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    • v.24 no.4
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    • pp.275-281
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    • 2011
  • $AlPO_4$-type material was synthesized by a reaction of $Al(OH)_3$ and H3PO4 with organic templates from wastewater of detergent manufacturer. The surface of material was coated with carboxylate groups by the reaction of succinic anhydride with surface amino groups which were formed by treatment of the material with APTMS. Powder XRD patterns showed the characteristic patterns of $AlPO_4$. Morphology of the material was examined using a SEM and the functional groups were investigated by FT-IR analysis. The surface charge of a aqueous suspension was analyzed: $AlPO_4-NH_2$ has positively charged surface while $AlPO_4$-COOH has negatively charged one. They were used for the removal of toxic metals from aqueous solution. The lead ions were adsorbed on the surface by the formation of complexes with carboxylate of surface and $K_d$ was 91.1 mL/g. In conclusion, the $AlPO_4$-COOH might be applicable in the removal of toxic metal ions from aqueous system.

Annual Effective Dose of 210Po from Sea Food Origin (Oysters and Mussels) in Korea

  • Cho, Boeun;Hong, Gi-Hoon;Kim, Suk Hyun;Lee, Hyunmi
    • Journal of Radiation Protection and Research
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    • v.41 no.3
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    • pp.245-252
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    • 2016
  • Background: Ingestion of $^{210}Po$ laden seafood accounts for a substantial amount of the effective dose of $^{210}Po$. Among seafood items, mollusks, especially domestically produced oysters and mussels, are highly enriched in $^{210}Po$ and are consumed in large quantities in Korea. Materials and Methods: Oysters and mussels around the Korean coasts were collected from major farm areas in November 2013. Samples were spiked with an aliquot of $^{209}Po$ as a yield tracer, and they were digested with $6mol{\cdot}L^{-1}$ $HNO_3$ and $H_2O_2$. The $^{210}Po$ and $^{209}Po$ were spontaneously deposited onto a silver disc in an acidic solution of $0.5mol{\cdot}L^{-1}$ HCl and measured using an alpha spectrometer. The activity concentrations of $^{210}Pb$ and $^{210}Po$ were decay corrected to the sampling date, accounting for the possible in-growth and decay of $^{210}Po$. Results and Discussion: $^{210}Po$ activity concentrations in oysters were in a range from 41.3 to $206Bq{\cdot}(kg-ww)^{-1}$ and mussels in a range from 42.9 to $46.7Bq{\cdot}(kg-ww)^{-1}$. The $^{210}Po$ activity concentration of oysters in the turbid Western coast was higher than the Southern coast. The $^{210}Po$ activity concentration of the oysters was positively correlated ($R^2=0.89$) with those of the suspended particulate matter in the surface water. The calculated annual effective dose of $^{210}Po$ from oysters and mussels consumed by the Korean population was 21-104 and $5.01-5.46{\mu}Sv{\cdot}y^{-1}$. The combined effective dose due to the consumption of oysters and mussels appears to account for about $35{\pm}19%$ of that arising from seafood consumption in the Korean population. Conclusion: The annual effective dose of $^{210}Po$ for oysters in the Korean population was found to be higher than other countries. The total annual effective dose of $^{210}Po$ due to consumption of oysters and mussels consumed in Korea was found to be $76{\pm}42{\mu}Sv{\cdot}y^{-1}$, accounting for $28{\pm}16%$ of the total effective dose of $^{210}Po$ from food in Korea.

Cellular Localization and Translocation of Duplication and Alternative Splicing Variants of Olive Flounder Phospholipase C-δ1 (넙치 3가지 타입 인지질가수분해효소(PLC-δ1)의 세포 내 위치 및 이동)

  • Kim, Na Young;Kim, Moo-Sang;Jung, Sung Hee;Kim, Myoung Sug;Cho, Mi Young;Chung, oon Ki;Ahn, Sang Jung
    • Journal of Life Science
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    • v.27 no.11
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    • pp.1369-1375
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    • 2017
  • The purpose of this study was to investigate the cellular characterization of phospholipase C-${\delta}1$ in olive flounders (Paralichthys olivaceus). In general, phospholipase C signaling pathways are distributed in nuclei at plasma membranes and in cytoplasms, although the pathways' nuclear localization mechanisms are unclear. P. olivaceus duplicates type-A PoPLC-${\delta}1$ (PoPLC-${\delta}1A$), which has a high similarity to the human isoform PLC-${\delta}$; type-B PoPLC-${\delta}1$ (PoPLC-${\delta}1B$ [Sf]), which has a low similarity to the human isoform PLC-${\delta}$ and the alternative splice variant PoPLC-${\delta}1B$ (Lf), which has a nuclear localization signal (NLS) and a nuclear export signal (NES) for nuclear imports and exports, respectively. This study confirmed the effects of the cellular localization and translocation of GFP-tagged PoPLC-${\delta}1A$, PoPLC-${\delta}1B$ (Sf) and PoPLC-${\delta}1B$ (Lf). It administered treatments of $Ca^{2+}$ ionophore ionomycin and endoplasmic reticulum (ER)-$Ca^{2+}$ pump inhibitor thapsigargin to hirame natural-embryo (HINAE) cells. A laser-scanning confocal microscope was used. GFP-tagged PoPLC-${\delta}1A$ was distributed to the cellular organelles, rather than to the cytoplasms and cytomembranes, when PoPLC-${\delta}1B$ (Lf) and PoPLC-${\delta}1B$ (Sf) were localized at the plasma membranes. The treatments of ionomycin and thapsigargin showed the accumulation of PoPLC-${\delta}1A$ in the nuclei when PoPLC-${\delta}1B$ (Lf) nucleocytoplasmic shuttling and PoPLC-${\delta}1B$ (Sf) nucleocytoplasmic shuttling were not observed. The results were the first evidence that PoPLC-${\delta}1A$, which contains functional, intact NES sequences, has a main role in nucleocytoplasmic shuttling and translocation in fish.

Anti-Complementary Properties of Polysaccharides Isolated from Fruit Bodies of Mushroom Pleurotus ostreatus

  • Kweon, Mee-Hyang;Jang, Hyo;Lim, Wang-Jin;Chang, Hyo-Ihl;Kim, Chan-Wha;Yang, Han-Chul;Hwang, Han-Joon;Sung, Ha-Chin
    • Journal of Microbiology and Biotechnology
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    • v.9 no.4
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    • pp.450-456
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    • 1999
  • A high molecular-weight water-soluble fraction(PO) obtained by the ethanol precipitation of 0.1 N NaOH extracts of the mushroom Pleurotus ostreatus showed 82% anti-complementary activity for complement consumption hemolysis. The PO consisted of 42% carbohydrate (w/w), 50% protein (w/w), and 3% uronic acid (w/w). Fifty-eight percent of the anti-complementary activity decreased by periodate oxidation and 22% by protease digestion, suggesting that the sugar and protein moieties are essential for this activity. Two polysaccharide fractions, PO-IIIa-1 and PO-IIIa-2, with anti-complementary activity were isolated from the PO using DEAE-Sepharose FF followed by Sephadex G-75 and Sepharose CL-6B gel permeation chromatographies. The PO-IIIa-2 was found by HPLC to be nearly homogeneous, with the molecular mass of 531 kDa, and showed 96% $ITCH_{50}$ (inhibition against the total complement hemolysis of deionized water as the control) at a concentration of 1 mg/ml. This fraction contained galactose, mannose, fucose, and glucose with molar ratios of 1.75:1:0.65 and 0.59, respectively. The majority of galactose and mannose units in the PO-IIIa-2 were composed of TGalp1 ->, ->6Galp1->, ->2,6Galp1->, and ->Manp1->. The PO-IIIa-1 (molecular mass of 2000 kDa), exhibiting higher activity than the PO-IIIa-2, was further purified into two fractions, unbound proteoglycan (PO-IIIa-1A) and bound glucomannan (PO-IIIa-lB), by affinity chromatography using ConA-Sepharose CL-4B. The anti-complementary activity of each affinity purified fraction decreased as compared to that of the native PO-IIIa-1 fraction, indicating that the formation of complex between both polysaccharide fractions was necessary for full anti-complementary activity.

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Studies on the Proteolytic Enzyme of Mold (Part I) Production and Heat Resistance of Acid Protease by Rhizopus japonicus S-62 (사상균의 단백질분해효소에 관한 연구 (제1보) Rhizopus japonicus S-62에 의한 산성 생산 및 내열성시험)

  • 정만재
    • Microbiology and Biotechnology Letters
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    • v.5 no.3
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    • pp.153-158
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    • 1977
  • These experiments were conducted to investigate the condition of the production and the heat resistance of the acid protease by Rhizopus japonicus S-62. The results obtained were as follows: 1) The optimum concentrations of sucrose, yeast, ammonium chloride and sodium phosphate monobasic added to the wheat bran medium in the acid protease production were 0.5%, 2.0%, 0.4%, and 0.4%, respectively. 2) KH$_2$PO$_4$ and NaH$_2$PO$_4$ were the most effective as the heat resistant agents. 3) When the enzyme solutions added with KH$_2$PO$_4$ and NaH$_2$PO$_4$ to the concentration of 2% were heated for 10 min, at 50$^{\circ}C$, their residual activities were 100%, respectively. 4) The heat resistant effects of KH$_2$PO$_4$ and NaH$_2$PO$_4$ were not observed almost above 55$^{\circ}C$.

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Preparation and Photoluminescence Properties of LiBaPO4:Eu2+ Phosphors by Solid State Reaction Method (고상반응법에 의한 LiBaPO4:Eu2+ 계 형광체의 제조 및 광 발광 특성)

  • Park, In Yong
    • Journal of the Microelectronics and Packaging Society
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    • v.26 no.4
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    • pp.83-88
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    • 2019
  • LiBaPO4:Eu2+ phosphors with stoichiometric and nonstoichiometric compositions were prepared using a solid state reaction followed by heat treatment in reduced atmosphere, and the crystal structures and photoluminescence(PL) properties of the powders were investigated by x-ray powder diffraction and luminescence spectrometer. At 900℃, the Ba3(PO4)2 phase as the intermediate phase was observed with the LiBaPO4 phase as the main crystalline phase. Samples with a low europium concentration at 1,000℃ belonged to the trigonal structure, whereas samples with Eu2+ content more than 4 mol% showed monoclinic structure. In the nonstoichiometric compositions of 4 mol% Eu2+ and above, a single phase of Eu2+-doped LiBaPO4, showing bluish green emission, was formed.

Management of Nutrient Solution Based on $\textrm{NH}_4\textrm{H}_2\textrm{PO}_4$Concentration in Deep Flow Culture of Cherry Tomato (방울토마토 담액재배시 $\textrm{NH}_4\textrm{H}_2\textrm{PO}_4$ 농도에 기초한 배양액 조절)

  • 이문정;김성은;김영식
    • Journal of Bio-Environment Control
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    • v.4 no.2
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    • pp.188-194
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    • 1995
  • This study was carried out to investigate the effect of NH$_4$H$_2$PO$_4$ on pH stabilization in deep flow culture system using tap water, and to determine the optimum range of NH$_4$H$_2$PO$_4$ in culture solution. The pH of tap water is 7.5. The higher the concentration of NH$_4$H$_2$PO$_4$ was, the more the pH of nutrient solution was decreased. In NH$_4$H$_2$PO$_4$ 4/3-5/3 me/$\ell$, the pH of nutrient solution was 6-7.5 during the experiment. The highest brix(%) was obtained in NH$_4$H$_2$PO$_4$ 5/3-6/3 me/$\ell$. Leaf length, leaf width and stem-base diameter were highest in NH$_4$H$_2$PO$_4$ 2/3 me/$\ell$. The L and b* values were highest and the a* value was lowest in NH$_4$H$_2$PO$_4$ 8/3 me/$\ell$. Toxicity symptom of ammonium appeared in NH$_4$H$_2$PO$_4$ 8/3 me/$\ell$. It suggests that there was the relationship between leaf color and growth condition. It was concluded that NH$_4$H$_2$PO$_4$ 2/3 me/$\ell$ was good before harvest stage and NH$_4$H$_2$PO$_4$ 5/3-6/3 me/$\ell$ at harvest stage.

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Electrochemical Characteristics of Carbon-coated LiFePO4 as a Cathode Material for Lithium Ion Secondary Batteries

  • Shin, Ho-Chul;Lee, Byung-Jo;Cho, Won-Il;Cho, Byung-Won;Jang, Ho
    • Journal of the Korean Electrochemical Society
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    • v.8 no.4
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    • pp.168-171
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    • 2005
  • The electrochemical properties of $LiFePO_4$ as a cathode for Li-ion batteries were improved by incorporating conductive carbon into the $LiFePO_4$. X-ray diffraction analysis and SEM observations revealed that the carbon-coated $LiFePO_4$ consisted of fine single crystalline particles, which were smaller than the bare $LiFePO_4$. The electrochemical performance of the carbon-coated $LiFePO_4$ was tested under various conditions. The carbon-coated $LiFePO_4$ showed much better performance in terms of the discharge capacity and cycling stability than the bare $LiFePO_4$. The improved electrochemical performances were found to be attributed to the reduced particle size and enhanced electrical conductivity of the $LiFePO_4$ by the carbon.

A Study on Key Management Technology for DRM (DRM에서 키 관리 기술에 관한 연구)

  • Yang, Seong-Hoon;Han, Dea-Yul;Kim, Seong-Hoon;Park, Ik-Su;Lee, Kyoung-Hyo;Jung, Seok-Won;Seo, Jae-Hyun;Kim, Jong-Hwa;Oh, Byeong-Kyun
    • Proceedings of the Korea Information Processing Society Conference
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    • 2005.05a
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    • pp.1177-1180
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    • 2005
  • DRM에서 키 관리 기술은 중앙 집중 키 관리 방식, Enveloping 방식 등이 있다. 본 논문에서는 암호화 키를 콘텐츠에 함께 동봉하는 키 관리 방식을 기반으로 새로운 DRM 키 관리 기술을 제안한다. 제안하는 키 관리 기술은 사용자 인증에서 사용된 인증정보를 콘텐츠 암호화 키로 사용함으로 콘텐츠 암호화 키를 암호화하여 전송할 필요가 없는 장점이 있다.

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