Studies on the Proteolytic Enzyme of Mold (Part I) Production and Heat Resistance of Acid Protease by Rhizopus japonicus S-62

사상균의 단백질분해효소에 관한 연구 (제1보) Rhizopus japonicus S-62에 의한 산성 생산 및 내열성시험

These experiments were conducted to investigate the condition of the production and the heat resistance of the acid protease by Rhizopus japonicus S-62. The results obtained were as follows: 1) The optimum concentrations of sucrose, yeast, ammonium chloride and sodium phosphate monobasic added to the wheat bran medium in the acid protease production were 0.5％, 2.0％, 0.4％, and 0.4％, respectively. 2) KH$_2$PO$_4$ and NaH$_2$PO$_4$ were the most effective as the heat resistant agents. 3) When the enzyme solutions added with KH$_2$PO$_4$ and NaH$_2$PO$_4$ to the concentration of 2％ were heated for 10 min, at 50$^{\circ}C$, their residual activities were 100％, respectively. 4) The heat resistant effects of KH$_2$PO$_4$ and NaH$_2$PO$_4$ were not observed almost above 55$^{\circ}C$.

Rhizopus japonicus S-62에 의한 acid protease의 생산조건 및 효소의 내열성을 검사하여 다음과 같은 결과를 얻었다. 1) 산성 protease의 생산에 있어 wheat bran medium에 관한 sucrose, yeast, ammonium chloride, sodium phosphate monobasic의 최적 첨가 농도는 각각 0.5%, 2.0%, 0.4%이었다. 2) 내열제로서KH$_2$PO$_4$와 NaH$_2$PO$_4$는 가장 효과적이었다. 3) 효소액에 KH$_2$PO$_4$와 NaH$_2$PO$_4$를 각각 2%씩 첨가하고 5$0^{\circ}C$에서 10분간 가열처리하였을 때 잔존청성은 다 같이 100%를 나타내었다. 4. 55$^{\circ}C$이상에서는 KH$_2$PO$_4$와 NaH$_2$PO$_4$의 과열변과는 거의 없었다.