• Journal of Microbiology and Biotechnology
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• 제31권1호
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• pp.92-103
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• 2021

Lactobacillus casei, one of the most widely used probiotics, has been reported to alleviate multiple diseases. However, the effects of this species on intestinal diseases are strain-specific. Here, we aimed to screen L. casei strains with inflammatory bowel disease (IBD)-alleviating effects based on in vitro physiological characteristics. Therefore, the physiological characteristics of 29 L. casei strains were determined, including gastrointestinal transit tolerance, oligosaccharide fermentation, HT-29 cell adhesion, generation time, exopolysaccharide production, acetic acid production, and conjugated linoleic acid synthesis. The effects of five candidate strains on mice with induced colitis were also evaluated. The results showed that among all tested L. casei strains, only Lactobacillus casei M2S01 effectively relieved colitis. This strain recovered body weight, restored disease activity index score, and promoted anti-inflammatory cytokine expression. Gut microbiota sequencing showed that L. casei M2S01 restored a healthy gut microbiome composition. The western blotting showed that the alleviating effects of L. casei M2S01 on IBD were related to the inhibition of the NF-κB pathway. A good gastrointestinal tolerance ability may be one of the prerequisites for the IBD-alleviating effects of L. casei. Our results verified the efficacy of L. casei in alleviating IBD and lay the foundation for the rapid screening of L. casei strain with IBD-alleviating effects.

• 동의생리병리학회지
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• 제16권5호
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• pp.989-1000
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• 2002

The purpose of this study is to examine the toxic effects caused by xanthine oxidase/hypoxanthine(XO/HX) and the effects of herbal extracts such as JHYJ and GJHYJ on the treatment of the toxic effects. For this purpose, experiments with the cultured hippocampal cells from new born mice were done. The results of these experiments were as follows. 1. XO/HX, a oxygen radical-generating system, decreased the survival rates of the cultured cells on XTT assay and INT assay, the amount of DNA syntheses, and the amount of neurofilaments, and increased the lipid peroxidation. 2. JHYJ and GJHYJ have the efficacy of increasing the survival rates of the cultured cells. 3. JHYJ and GJHYJ have the efficacy of increasing the amount of neurofilaments and of decreasing the lipid peroxidation. 4. JHYJ and GJHYJ have the efficacy of increasing the amount of DNA syntheses. From the above results, it is suggested that Jihwangyeumja and Gamijihwangyeumja have marked efficacy as a treatment for the damages caused by the XO/HX-mediated oxidative stress. And Jihwangyeumja and Gamijihwangyeumja are thought to have certain pharmacological effects. Further dinical study of this pharmacological effects of Jihwangyeumja and Gamijihwangyeumja should be complemented.

• 동의생리병리학회지
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• 제17권6호
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• pp.1427-1432
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• 2003

This study was carried out to investigate the antimicrobial and anticancer effects of Galla Rhois (GR) on pathogens isolated from oral and KB human oral epidermoid carcinoma cells. Their antimicrobial activities were tested against Streptococcus mutans (S. mutans), Staphylococcus aureus (S. aureus), Enterobacter aerogenes (E. aerogenes), Escherichia coli (E. coil) and Candida albicans (C. albicans). GR powder has the antimicrobial activity against C. albicans, S. mutans and S. aureus. The extracts of water and ethanol have the antimicrobial activity against S. sureus and C. albicans. The water extract showed inhibitory activity against the growth and pH of above mentioned reference microorganisms. The water extract of GR declined cell viability in a dose dependent manner. DNA flow cytometric analysis showed that population of sub-G/sub 0//G₁, phase of cell cycle was increased by GR extract treatment in a dose dependent manner. Western blot analysis revealed that Caspase-3 was reduced by GR extract treatment. These result suggested that GR has the effect of antimicrobial on pathogens isolated from oral, and also, has anticancer effect that associated with the induction of apoptosis in a dose dependent manner in KB human oral epidermoid carcinoma cells. It may be GR-induced apoptosis was mediated via activation of Caspase-3.

• 지역사회간호학회지
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• 제20권1호
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• pp.75-86
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• 2009

Purpose: This study has a purpose to suggest research and intervention directions for health promoting behaviors (HPB) of the Korean middle-aged in the future. Methods: Forty four articles on HPB of the Korean middle-aged were reviewed and analyzed. The articles analyzed are theses written for a doctoral or master's degree and published between 1995 and 2007. Results: 1. As for the major characteristics of the subject groups, most of them were between 40 and 60 years old (61.4%), were females (79.5%), and resided in cities (84.1%). 2. The dependent variables adopted were physiological, psychological and cognitive factors (self-efficacy, knowledge of health management, etc.), HPB and factors related to Health Promoting Lifestyle Profile (HPLP). 3. The average scores of HPLP were $1.57{\sim}3.08$ on a 4-point scale. The highest score was observed in self-actualization, and the lowest score in exercise. 4. Self-efficacy, perceived health status and income had significant correlations with HPB. 5. In regression analysis, self-efficacy, self-esteem and perceived health status were the most powerful predictors. Conclusion: It is necessary to develop intervention tools for more diverse HPB of the Korean middle-aged. The interventions are recommended to focus on increasing exercise and to use strategies for improving self-efficacy and self-esteem.

• 대한간호학회지
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• 제50권1호
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• pp.52-65
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• 2020

Purpose: To examine the effects of a smartphone application-based exercise program on self-efficacy expectations (SEE) and outcome expectations regarding exercise (OEE), physical fitness, activity level, physiological indices, and health-related quality of life in a sample of hemodialysis patients. Methods: A quasi-experimental control group pre-test post-test design was used. Subjects were recruited from two university hospitals in G city. The subjects were assigned randomly by coin toss: 33 participants to the experimental group and 30 to the control group. A literature review and the self-efficacy theory were used to develop the smartphone program. Experts designed and verified the program to be userfriendly and in consideration of user interaction. Data were collected through a self-report pre-test post-test questionnaire and online medical records. Results: In the experimental group, the levels of physical fitness and physical activity were significantly improved post-test, but the scores on health-related quality of life and the physical indices did not improve. In the experimental group, the SEE and OEE post-test scores were also significantly higher than the pre-test scores, but the control group's scores did not change. Conclusion: The smartphone application-based exercise program based on self-efficacy theory significantly improved the level of physical fitness and activity, SEE, and OEE for hemodialysis patients. The use of this application-based exercise program for hemodialysis patients might be an effective nursing intervention tool for improving SEE, OEE, level of physical fitness, and physical activity.

• 근관절건강학회지
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• 제4권1호
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• pp.1-14
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• 1997

It is reported that self-help education is effective to increase self-efficacy for the patients with chronic illness. Also self-efficacy Is proved to be an Important variable to effect the physical and psychological health state. Based upon this theoretical framework, KRHPS (Korean Rheumatology Health Professions Society) has administered for 2 years the self-help education increasing self-efficacy for the patients with arthritis. The group had a weekly session for 6 weeks. During the session, self-efficacy was increased by 1) actually performing a behavior, 2) listening to other patients talking about a successful experience or seeing other patients performing a behavior, 3) Instructor's or another patient's verbal persuasion, and 4) physiological cues experienced by themselves. Every group had 4-15 participants and 2 instructors. Data were collected by self-administered questionaire for 211 out of 361 participants. One hundred and fifty two subjects were analyzed because of missing values. As a result, it was confirmed that self-help education was effective to increase self-efficacy and self-efficacy was an important variable to Improve pain, depression, and ADL. Also it may fairly be said that nurses were right health-professionals to lead self-help groups for the patients with chronic illness. So it is important to facilitate many other self-help education for the patients with chronic illness.

• 한국보건간호학회지
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• 제25권1호
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• pp.5-16
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• 2011

Objectives: This study analyzed the effects of diabetes interventions published in journals of nursing, medical and public health, to suggest directions for future research. Methods: A comprehensive search of three databases was undertaken (Korean studies, Riss4U, RICHIS) using research terms such as "diabetes", "nursing", "public health", "intervention" and "program". One hundred fifty four original articles were retrieved and 21 articles were finally analyzed. The analyzed articles satisfied the inclusion criteria and their effect size was calculated. Results: Most studies about diabetes intervention were published form 2001. 2005 (57.1%). The psycho-social variables, 'self-efficacy' and 'self-care' presented good effects on exercise or exercise prescription intervention. In addition, the physiological variables, 'fasting blood sugar' and 'glycosylated glucose' presented good effects on the interventions using four more elements or the interventions held in a public health center. Conclusion: Further research should consider the essential elements that benefit diabetes patients' behaviors or physiological indicators.

• BMB Reports
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• 제47권2호
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• pp.51-59
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• 2014

Cellular senescence is a physiological process of irreversible cell-cycle arrest that contributes to various physiological and pathological processes of aging. Whereas replicative senescence is associated with telomere attrition after repeated cell division, stress-induced premature senescence occurs in response to aberrant oncogenic signaling, oxidative stress, and DNA damage which is independent of telomere dysfunction. Recent evidence indicates that cellular senescence provides a barrier to tumorigenesis and is a determinant of the outcome of cancer treatment. However, the senescence-associated secretory phenotype, which contributes to multiple facets of senescent cancer cells, may influence both cancer-inhibitory and cancer-promoting mechanisms of neighboring cells. Conventional treatments, such as chemo- and radiotherapies, preferentially induce premature senescence instead of apoptosis in the appropriate cellular context. In addition, treatment-induced premature senescence could compensate for resistance to apoptosis via alternative signaling pathways. Therefore, we believe that an intensive effort to understand cancer cell senescence could facilitate the development of novel therapeutic strategies for improving the efficacy of anticancer therapies. This review summarizes the current understanding of molecular mechanisms, functions, and clinical applications of cellular senescence for anticancer therapy.

• 한국산학기술학회논문지
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• 제17권6호
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• pp.495-509
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• 2016

이 연구는 노인의 건강증진태도와 생리적 지수에 대하여 클린다이어트 프로그램의 중재효과를 파악하고자 비동등성 대조군 전 후 실험설계로 2015년 6월 24일부터 2015년 9월 18일까지 12주간 실시되었다. 연구대상자는 대구광역시 C 노인대학에서 만성질환이 있는 65세 이상 노인 60명을 선정하여 실험군과 대조군으로 각각 30명씩 배정하였다. 클린다이어트 프로그램은 실험군에만 적용되었으며 웰니스, 건강행동, 주관적 건강감, 우울 및 생리적 지수(혈압, 혈당, TG/TC, BMI)에 대하여 실험군과 대조군의 사전 사후 차이를 비교하였다. 연구결과 실험군의 웰니스(t=7.069, p<.001), 건강행동(t=6.196, p<.001), 주관적 건강감(t=6.624, p<.001) 수준이 향상되었고, 우울(t=4.038, p<.001)은 완화된 변화를 보였다. 그리고 생리적 지수는 BMI(t=5.974, p<.001)에서만 감소된 것으로 분석되었다. 이와 같은 결과는 클린다이어트 프로그램의 중재효과를 입증한다. 따라서 노인에게 클린다이어트 프로그램을 적용하면 삶의 질을 높이는 총체적인 생활방식이나 건강을 유지하기 위한 생활양식 그리고 우울증 완화와 BMI관리에 효율적인 것으로 판단된다.

• 대한간호학회지
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• 제30권4호
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• pp.995-1005
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• 2000

This study was performed to identify the physiological and psychological variables related to successful weaning from a mechanical ventilator. The subjects of this study were 22 patients who received mechanical ventilation therapy for more than 3 days in intensive care units. Before the weaning trial, baseline data for following physiologic variables were obtained: spontaneous respiration rate, blood pressure, pulse rate, PaO2, PaCO2, PEEP, static compliance, minute ventilation, tidal volume, rapid shallow breathing index(f/VT), SaO2, PaO2/FiO2 and mean arterial pressure. During spontaneous breathing, physiologic and psychologic variables such as vital signs, ABG, perspiration, chest retraction, paradoxical respiration, dyspnea, anxiety, confidence and efficacy were measured. Successful weaning was defined as sustaining spontaneous respiration over 24 hours after extubation. Weaning failure was defined as the development of more than one of following signs: (1) hypoxemia, (2) CO2 retention or (3) perspiration, tachypnea, chest retraction, tachycardia, arrhythmia, hypotension or hypertension. Subjects (N=18) who successfully weaned from mechanical ventilator were compared with subjects (N=4) who failed. The results are as follows; Eighteen percents of the subjects failed during the weaning trial. Most subjects in the failed group were mechanically ventilated for long-time. This result shows that the success of weaning is more difficult in long-term ventilation patients. In the baseline data that was measured before weaning trial, the mean score of PaO2 in the successfully weaned group was 121mmHg. This is significantly higher than the mean score of PaO2 in the failed group(95mmHg). However, the scores of pH, tidal volume, f/VT, pulse rates, blood pressure, mean airway pressure, SaO2, and PaCO2 were similar between the two groups. Specially the scores of f/VT index as a predominant predictor for successful weaning were not significant (f/VT=44.4) and (f/VT=47). During spontaneous breathing, the scores of dyspnea and anxiety level in the successfully weaned group were less than those of the failed group. On the contrary, the scores of confidence and efficacy in the successful group were greater than those of the failed group. In conclusion, the baseline data that were measured before weaning trial were similar between the both groups, therefore future studies are needed to focus on searching other variables besides physiological parameters related to weaning outcome.