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Effect of Methionine Sulfoximine in nitrogenase activity by ammonia and glutamine in Rhodopseudomonas sphaeroides (암모니아와 glutamine에 의한 Rhodopseudomonas sphaeroides의 질소 고정 효소활성에 미치는 Methionine Sulfoximine의 영향)

  • 이혜주
    • Korean Journal of Microbiology
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    • v.26 no.3
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    • pp.215-222
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    • 1988
  • The effect of ammonia and glutamine on nitrogenase activity of Rhodopseudomonas sphaeroides was examined. The nitrogenase activity of this strain was inhibited by ammonia and glutamine. When ammonia and glutamine were exhausted, nitrogenase activity promptly resumed at its original rate. Methionine sulfoximine (MSX), irreversible glutamine synthetase (GS) inhibitor, is a structural analogue of glutamate. MSX was used in order to know whether the nitrogenase activity was inhibited by ammonia and glutamine directly or not. The ability of MSX to prevent nitrogenase switch-off by ammonia was found to be dependent upon the phase of culture. When the cells were sampled after 12 hour culture, $500{\mu}M$ MSX would not prevent the nitrogenase switch-off by ammonia. Twenty one percents of GS actibity was inhibited by $500{\mu}M$ of MSX and concentration of released ammonia decreased. But nitrogenase activiy was still inhibited by ammonia. However, nitrogenase switch-off after 20 hours would be prevented by $100{\mu}M$ of MSX. On the other hand, GS activity was ingibited completely by $100{\mu}M$ MSX and concentration of released ammonia somewhat increased. But nitrogenase activity was not inhibited. The data indicated that the inhibition of in vivo nitrogenase actibity of Rp. sphaeroides by ammonia seemed to be mediated by products of ammonia assimilation rather than by ammonia itself.

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Inhibitory Effect of Methanol Extracts and Solvent Fractions from Doenjang on Mutagenicity Using in vitro SOS Chromotest and in vivo Drosophila Mutagenic System (된장 메탄올 추출물 및 분획물에 의한 in vitro SOS Chromotest 실험계와 in vivo 초파리 돌연변이 검출계에서의 항돌연변이 효과)

  • Lim, Sun-Young;Lee, Sook-Hee;Park, Keun-Young;Yun, Hee-Sun;Lee, Won-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.9
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    • pp.1432-1438
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    • 2004
  • This study investigated the inhibitory effect of methanol extracts and several solvent fractions from doen-jang on mutagenicity using in vitro SOS chromotest and in vivo Drosophila mutagenic system. In order to determine an antimutagenic effect of doenjang methanol extracts, other soybean fermented foods and original materials were compared. The treatment of doenjang methanol extracts (100 ${\mu}$/assay) to SOS chromotest system inhibited N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced mutagenicity by 87~97% and showed higher antimutagenic effect than other fermented foods. Among solvent fractions from doenjang methanol extracts, the ethylacetate and dichloromethane fractions showed the stronger antimutagenic effect (91% and 95%, respectively) in SOS chromotest. In Drosophila mutagenic system, the treatment of ethylacetate fraction (5%/bottle) significantly inhibited aflatoxin $B_1$ induced mutagenicity by 97%. These results demonstrated that doenjang had an inhibitory effect to mutagenic agents in both in vitro and in vivo mutagenic systems, suggesting that its antimutagenic effect may be due to active compounds in the ethylacetate fraction from doenjang methanol extracts.

Deterioration Analysis and Source Area on Rock Properties of the Seokgatap Pagoda in the Bulguksa Temple, Korea (불국사 석가탑의 풍화훼손도 분석 및 기원암의 산지추정)

  • Lee, Myeong-Seong;Lee, Chan-Hee;Suh, Man-Cheol;Choi, Seok-Won
    • 한국문화재보존과학회:학술대회논문집
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    • 2004.10a
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    • pp.15-24
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    • 2004
  • The Seokgatap pagoda composed of mainly alkali granite and other minor pink-feldspar granite, fine-grained granite, granodiorite, diorite, gabbro, and tuff. Despite the small loss and damage derived from joints, its peel-off and exfoliation are serious enough to cause the heavy deterioration on the stone surface. The chemical and petrological weathering has partly replaced the original rock-forming minerals with clay minerals and iron oxyhydroxides. Based on the petrogenesis, rock materials of the pagoda is very similar to rocks of Dabotap pagoda and the Namsan granite in the Gyeongju. The central fart of the pagoda has sunken highly, which caused all the corners to split and the structural transformation to become worse. The reverse V-shaped gaps between the materials have broken stones filled in a coarse way. The iron plates inserted between the upper flat stone laid on other stones and tile pagoda body in the north and east side has been exposed in the air and corroded, discoloring of the adjacent stones. The overall diagnosis of the Seokgatap pagoda is the deteriorated functions of the stone materials, which calls for a long-term monitoring and plans to reinforce the stone surfaces. But the main body including the pagoda roof stone needs washing on a regular basis, and the many different cracks should be fixed with glue by using the fillers or hardeners designed for stone cultural properties after removing the cement mortar. In case of the replacement of the stone materials with new stones, it's necessary to examine the pagoda for the center of gravity and support intensity of the materials. The structural stability of the pagoda can be attained by taking a reinforce measure in geotechnical engineering and making a drainage. The ground humidity, which has aggravated weathering and structural instability, should be resolved by setting up a humidity reduction facility. The contamination of lichens and bryophyte around the pagoda and on the surface is serious. Thus biochemical treatments should be given too in order to prevent further biological damages and remove the vegetation growing on the discontinuous planes.

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Effect of $SO_2$ on DeNOx by Ammonia in Simultaneous Removal of SOx and NOx over Activated Coke (활성 코우크스상의 동시 탈황탈질에서 암모니아에 의한 탈질에 이산화황이 미치는 영향)

  • Kim, Hark-Joon;Yoon, Cho-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.2
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    • pp.201-208
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    • 2010
  • The $SO_2$ and $NO_x$ removal with an activated coke catalyst was conducted by a two-stage reaction which first $SO_2$ was oxidized to $H_2SO_4$ and then $NO_x$ was reduced to $N_2$. But if unreacted sulfur dioxide entered in the second stage, the $NO_x$ reduction was hindered by the reaction with ammonia. In this study, experimental investigations by using lab-scale column apparatus on the product and the reactivity of $SO_2$ with ammonia over coke catalyst which was activated with sulfuric acid was carried out through ultimate analysis DTA, TGA and SEM of catalyst before and after the reaction. Also, the effect of reaction emperature on the reactivity of $SO_2$ with ammonia was determined by means of breakthrough curves with time. The obtained results from this study were summarized as following; Activated cokes were decreased carbon component and increased oxygen and sulfur components in comparison with original cokes. The products over coke catalyst were faced fine crystal of $(NH_4)_2SO_4$, which results in the pressure loss of reacting system. The order of general reactivity in terms of the reaction temperature after breakthrough for $SO_2$ was found to be $150^{\circ}C$ > $200^{\circ}C$ > $100^{\circ}C$. This was related to adsorption amounts of ammonia on the activated cokes.

The Influence of Nano Synthesized Polymer Paint on Durability of Concrete (나노합성 무기질 폴리머계 표면처리제가 콘크리트의 내구성에 미치는 영향)

  • Beak, Jong-Myeong;Park, Youg-Keol
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.20 no.1
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    • pp.56-63
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    • 2016
  • This experiment was compared and analyzed between the original surface paint through chloride penetration, neutralization, freeze-thaw and chemical corrosion resistance measuring internal structure and volume of voids in order to evaluate the effect of increase in durability of the newly modeled nano synthesized polymer paint painted on concrete surface which results improvement on air permeability to increase the durability of concrete structures. The test result of measuring volume of void and inner structure, concrete, spreaded with nano synthesized polymer paint, showed decreasing trend of pore volume in the range of less than $0.1{\mu}m$ and more than $0.3{\mu}m$. Also, using an electron microscope inside showed tightness of hydration texture. Chloride penetration depth of concrete, painted with nano synthesized polymer paint, was decreased more than 92% compared to non-painted concrete and 70% with water-based epoxy painted concrete. Especially, chemical corrosion resistance test set with aqueous solution of 5% sulfuric acid, non-painted concrete and water-based epoxy painted concrete showed weight loss of 4% after dipping for 12 days. On the other hand, concrete painted with nano synthesized polymer paint showed 1.7% weight loss under the same condition. Also, it showed great result of appearance under the criteria of Tsivilis et al.

Reinterpretation of the Seowon Architecture of Sarim in Choseon Dynasty: Evaluating the Seowon of the Kiho School (조선시대 사림의 서원건축 재해석: 기호학파 서원에의 가치부여)

  • Lee, Hee-Bong;Sohn, Bong-Kyun
    • Journal of architectural history
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    • v.16 no.6
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    • pp.121-140
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    • 2007
  • Seowon(書院) is a representing institution in Choseon Dynasty not only educationally but also politically and economically. Due to the artificial crackdown by Daewon-gun in 1871 and destruction by wars, it is difficult to restore and interpret the Seowon accurately at present. It is well known that the 'Basic Form' of the Seowon consists of an inner court, enclosed by the rear gangdang (lecture hall), dongseo-jae (east and west dormitory), and the front munlu (gate pavilion or upper story bower for relaxation), represented by so called 'front-dormitory rear-lecture-hall type', that is, 'jeonjae hudang(前齋後堂) type'. However, it is overlooked that this Basic Form is a product of Youngnam School located only in Youngnam area. A different form, of 'front-lecture-hall rear-dormitory type', or 'jeonndang hujae(前堂後齋) type' is located only in Gyeonggi, Hoseo and Honam area. It has been wrongly analyzed that the type is a result of the later period, emphasizing the memorial service rather than the lecture itself, and worshipping loyalists than Confucian scholars. Analysis encompassing each Seowon architecture has been mistakenly made by historians as "deterioration" of the original educational purpose of the Seowon from the early period to the later period. This paper raises the fact that the form of jeondang hujae type has been established since the early period of Seowon in the 16th Century. It has a unique order of space itself. Here, the lecture hall faces toward sadang (shrine). The inner court, enclosed by east and west jae and the lecture hall, becomes the outer yard of the shrine, and as a result two main spaces of the shrine and lecture area is merged into one. While the munlu of the basic type encloses the inner court of the main area, the munlu of jeondang hujae type is located at the vicinity area. This paper reinterprets the jeondang hujae type not as a form coming out of degenerated period but as a type that belongs to a different Confucian school, the area of Kiho, and concludes that the Seowon is a product of political struggles between the two schools and of the local economical situation. Each school has maintained his own type of form, therefore the remaining Kiho Seowon architecture can be reevaluated.

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Micro-crack Detection in Polycrystalline Solar Cells using Improved Anisotropic Diffusion Model (개선된 비등방 확산 모델을 이용한 다결정형 솔라셀의 마이크로 크랙 검출)

  • Ko, JinSeok;Rheem, JaeYeol
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.5
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    • pp.183-190
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    • 2013
  • In this paper, we propose an improved anisotropic diffusion model for micro-crack detection in heterogeneously textured surface of polycrystalline solar wafers. Due to the nature of the image sensor, the gray-level of the diagonal micro-crack is non-uniform. Thus, the conventional algorithms can't fully detect diagonal micro-cracks when the number of iteration is not enough. However, the increasing of the iteration number leads to increase computation time and detects micro-crack thicker than the original micro-crack. In order to overcome this drawback, we use the gradient of north, south, east, and west directions as well as extended directions. To calculate the diffusion coefficients, we compare the gradients of conventional directions and extended directions and apply the larger gradient values to the coefficient function. This is because the proposed method reflects the information of diagonal micro-crack. Comparing to Tsai et al.'s and Ko and Rheem's, the proposed algorithm shows superior efficiency in detecting the diagonal micro-cracks with less iterations in the images of polycrystalline solar wafers. In addition, it also shows that the thickness of segmented micro-crack is similar to the orignal micro-crack.

Properties of rin Resistance of High Performance Concrete with Varying Contents of Polypropylene Fiber and Specimen Size (폴리프로필렌 섬유의 혼입률 및 부재크기 변화에 따른 고성능 콘크리트의 내화 특성)

  • 한천구;양성환;이병열;황인성;전선천
    • Journal of the Korea Concrete Institute
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    • v.14 no.4
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    • pp.449-456
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    • 2002
  • Recently, the application of high strength and high performance concrete has been gradually increased as an important construction material for high rise and huge scaled construction. However, high performance concrete has undesirable characteristics of spalling subjected to high temperature due to its dense microstructure content. A spalling by fire brings surface failure and falling off concrete member. It is considered that spalling by fire should be taken into account for the safety of the concrete structure under fire. Therefore, in this paper, tests are carried out using high performance concrete containing polypropylene(PP) fiber in order to improve the fire resistance performance. PP fiber contents and member sizes are varied. According to experimental results, as for the influence of PP fiber contents, all the test specimens without PP fiber show entire failure in W/C of 35%, while they show nearly sound shape except some kinds of surface fracture in W/C of 55%. When PP fiber is contained more than 0.07%, favorable prevention effects of spatting by fire are obtained. As for the effects of test specimens size, it tends to increase the possibilities of spatting by fire as test specimens become larger. And spatting by fire at the edge of test specimens occurs more frequently than at the surface of test specimens. Residual compressive and tensile strength shows 45∼65 % of its original strength at W/C of 35%, and 30∼40% at W/C of 55 %.

Reliability and Validity of the Korean Version of SERVPERF : A Validation Study (서비스 질 척도(서브퍼프)의 타당화 연구: 종합사회복지관을 대상으로)

  • Kim, Yong-Seok;Choi, Jong-Bog;Hwang, Seong-Hye;Kim, Min-Suk;Seo, In-Ja
    • Korean Journal of Social Welfare
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    • v.61 no.2
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    • pp.107-135
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    • 2009
  • The purpose of this study is to develop and evaluate the Korean version of the SERVPERF which has been widely used in order to measure service quality in various kinds of fields including social work. A total of 328 adults using social welfare centers in Seoul and Gyeonggi-do participated in this study. Confirmatory factor analysis confirmed that the Korean version of the SERVPERF consists of 5 dimensions(tangibles, reliability, responsiveness, assurance, and empathy) like the original SERVPERF. 5 dimensions of the Korean version of the SERVPERF have acceptable levels of internal consistency. Its convergent validity and discriminant validity are satisfactory. Finally, weak or non-significant correlations between 5 dimensions of the Korean version of the SERVPERF and social desirability scale indicated that the participants' reponses to the Korean version of the SERVPERF were not affected by social desirability.

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A Proposal of Flow Limit for Soils at Zero Undrained Shear Strength (흙의 비배수전단강도가 0이 되는 함수비인 흐름한계의 제안)

  • Park, Sung-Sik;Nong, Zhenzhen
    • Journal of the Korean Geotechnical Society
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    • v.29 no.11
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    • pp.73-84
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    • 2013
  • When a slope failure or a debris flow occurs, a shear strength on failure plane becomes nearly zero and soil begins to flow like a non-cohesive liquid. A consistency of cohesive soils changes as a water content increases. Even a cohesive soil existing at liquid limit state has a small amount of shear strength. In this study, a water content, at which a shear strength of cohesive soils is zero and then cohesive soils will start to flow, was proposed. Three types of clays (kaolinite, bentonite and kaolinite (50%)+bentonite (50%)) were mixed with three different solutions (distilled water, sea water and microbial solution) at liquid limit state and then their water contents were increased step by step. Then, their undrained shear strength was measured using a portable vane shear device called Torvane. The ranges of undrained shear strength at liquid and plastic limits are 3.6-9.2 kPa and 24-45 kPa, respectively. On the other hand, the water content that corresponds to the value of the undrained shear strength changing most rapidly is called flow water content. The flow limit refers to the water content when undrained shear strength of cohesive soils is zero. In order to investigate the relationship between liquid limit and flow limit, the cohesive index was defined as a value of the difference between flow limit and liquid limit. The new plasticity index was defined as the value of difference between flow limit and plastic limit. The new liquidity index was also defined using flow limit. The values of flow limit are 1.5-2 times higher than those of liquid limit. At the same time, the values of new plasticity index are 2-5.5 times higher than those of original plasticity index.