• 한국토양비료학회지
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• 제39권4호
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• pp.195-203
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• 2006

길항미생물 MP-1은 여러 종의 곰팡이와 효모 및 음식물을 부패시키는 세균 (그람양성 및 음성) 에 대하여 항균활성을 보였다. MP-1의 16S rRNA gene 염기서열은 기존에 밝혀진 Brukholderia sp.의 염기서열과 99-100% 유사하였다. 배양액으로부터의 항균물질은 에틸아세테이트 (EtOAc)를 사용하여 분리하였으며 EtOAc에 용해된 산성 분획은 silica gel, Sephadex LH-20, ODS 컬럼크로마토 그래피 및 HPLC를 사용하여서 정제를 실시 하였다. 최종적으로 Gas chromatographic-mass 스펙트럼을 통한 분석을 통하여 4 종의 항균 활성을 나타내는 물질은 phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid 및 4-hydroxyphenylacetate methyl ester로 확인 되었다. 각각의 항균 활성물질들의 미생물에 대한 최소저해농도(MIC)는 $250{\sim}2500ug\;ml^{-1}$ 인 것으로 나타났다. 또한 6 mm paper disc 에서의 1mg (건물중) 산성 분획의 항균활성은 같은 비율로 혼합한 표준혼합용액 보다 더 효과적인 것으로 나타났다.

• 대한임상검사과학회지
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• 제52권3호
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• pp.221-228
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• 2020

Ochrobactrum anthropi는 oxidase를 생산하는 비발효산소성 그람음성 막대균으로 외관이 비슷하고 oxidase가 양성인 비발효 세균과 혼합배양 시 구분이 힘들고 생화학적 동정 장비로는 정확한 동정에 한계가 있다. 따라서 본 연구에서는 생화학적 검사 방법으로 동정이 힘든 세균동정의 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Platform (MALDI-TOF) 법의 유용성을 제시하고자 하였다. MicroScan을 이용해 검사했던 O. anthropi 5례를 분석한 결과, 최종보고까지 6.2일이 소요되었으며 E. coli의 3.0일에 비해 3.5일이 더 소요되었다. 5번 환자 고름 검체는 Achromobater xylosoxidans와 혼합감염으로 여러 번의 계대 배양과 재검사로 인해 11.3일이 소요되었는데, MALDI-TOF법으로 검사한 경우 한 번에 동정되었다. 4명의 환자는 기저질환이 있는 60세 이상이었고 기회감염과 원내감염의 가능성을 배제할 수 없었으며, 그 중 92일 만에 채취된 검체는 imipenem과 meropenem에 내성이었다. 따라서 O. anthropi처럼 동정이 까다로운 세균은 신속하고 적절한 환자 치료를 위해 MALDI-TOF법을 이용한 검사가 매우 유용할 것으로 사료된다.

• 대한지역사회영양학회지
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• 제8권6호
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• pp.927-937
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• 2003

This study was conducted to investigate dietary and other factors affecting bone mineral density (BMD) in Korean premenopausal women. Seventy-eight premenopausal women who visited the Health Promotion Center for health examinations volunteered to participate in this study. They were divided into two groups according to their bone status as shown by their T-scores: a non-osteoporotic group and a osteoporotic group. The results are as follows: The mean BMDs of the lumbar spine and femoral neck were 1.21$\pm$0.02$g/cm^2$ and 0.97$\pm$0.04$g/cm^2$, respectively. The BMD levels of the osteoporotic group were significantly lower than those of the non-osteoporotic group (p<0.001, respectively). The heights of the women in the osteoporotic group were significantly lower than those of the non-osteoporotic group (p<0.01) however, their body weights did not show any significant differences although they tended to be lower. The mean daily intake of energy was 1720$\pm$52㎉. When the nutrient intake was compared with the Korean recommended dietary allowances (RDA), calcium, Fe, vitamin A and riboflavin intakes were lower than the RDA. Their was no significant difference in the nutrient intake of the non-osteoporotic group and osteoporotic group except for the intakes of protein, fat and niacin. Their was no significant difference between the non-osteoporotic group and the osteoporotic group and all were within the normal range. However, the serum alkaline phosphatase level of the osteoporotic group was significantly higher than that of the non-osteoporotic group (p<0.001). Height measurements showed positive correlations with lumbar spine bone mineral density (LBMD, r=0.332, p<0.01) however there was no correlation with femoral neck bone mineral density (NBMD). Age, age at menarche, body weight, body mass index (BMI) and obesity showed no correlation with BMD. The BMD of the lumbar spine was significantly and positively related to the intake of niacin and vitamin C (r=0.236, p<0.05; r=0.274, p<0.05). Serum levels of calcium and phosphorus showed negative correlations with LBMD (r=-0.698, p=0.0001, r=-0.503, p=0.0001, respectively). The results suggested that the BMD of the lumbar spine was positively related to the intake of niacin and vitamin C in premenopausal women. Therefore, this study confirmed that one of the most effective ways to minimize bone loss would be have a higher intake of niacin and vitamin C rich foods and engaging habitually in physical activity may have a beneficial effect on BMD in the Premenopausal Period.

• 한국지구과학회지
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• 제30권6호
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• pp.734-743
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• 2009

본 연구는 부산지역의 먼지 농도와 황사발원지의 기후학적 요소를 주기분석 함으로써 부산지역의 먼지농도 주기에 영향을 주는 기후학적 조건, 특히 황사 발생과의 관련성에 대하여 고찰하였다. 부산지역에서 관측된 먼지 농도 시계열 자료를 이용한 단일스펙트럼분석을 수행한 결과 배출량이나 강수 효과 등에 의해 나타난 일반적인 주기(1년, 7일) 이외에, 상대적으로 3-4년 주기가 우세하게 나타났으며, 먼지 농도와 부산지역의 기상자료 및 황사발원지에서의 풍속과의 교차스펙트럼 분석을 통한 주기 분석을 수행해 본 결과, 3-4년 주기일 때 먼지농도와 풍속, 기압은 양의 상관관계, 기온, 상대습도와는 각각 음의 상관관계가 나타났다. 이는 황사 발생 조건과 잘 부합되는 것으로 나타나, 3-4년의 먼지 농도 주기는 황사의 장거리 수송과 관련이 있는 것으로 판단된다. 또한 황사 발생은 발원지의 지표 상태에 따라 발생빈도가 달라지므로, 여러 기후학적 요소들 중 황사발원지에서의 강수량과의 주기 분석을 수행해 본 결과, 발원지에서의 강수량 그 자체보다는 발원지에서의 가뭄지수(EDI)의 시계열이 우리나라 먼지 농도의 3-4년 주기와 더 연관이 있는 것으로 나타났고 이는 기후학적으로 황사 발원지에서의 지표건조 특성의 변동성과 연관이 있는 것으로 나타났다.

• 생명과학회지
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• 제28권11호
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• pp.1301-1315
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• 2018

참담치(Mytilus coruscus)의 혈구 유래의 항균 펩타이드를 역상 column들을 사용한 reversed-phase high-performance liquid chromatography (RP-HPLC)로 분리 및 정제하였다. 정제된 펩타이드는 matrix-assisted laser desorption ionization time-of-flight mass spectrophotometer (MALDI-TOF/MS)를 통해 분자량이 4041.866 Da으로 밝혀졌으며 Edman degradation법을 통해 25개의 N-말단 서열을 확보하였다. 이는 참담치의 mytilin B precursor와 100%, mytilin 8 precursor, mytilin 4 precursor와 96% 일치하였다. 또한 103개의 아미노산 서열을 코딩하고 있는 312 bp의 open-reading frame (ORF)을 밝혔으며 이는 참담치의 mytilin B precursor와 100% 일치하였다. 밝혀진 분자량과 아미노산 서열을 바탕으로 C-말단 alanine 잔기의 유무에 따라 2개의 펩타이드를 합성하였으며 이는 mytilin B1과 B2라고 명명하였다. 이들은 그람 양성 균주 Bacillus cereus, Streptococcus parauberis [minimal effective concentrations, MECs $41.6-89.7{\mu}g/ml$], 그람 음성 균주 Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Providencia stuartii, Pseudomonas aeruginosa, Vibrio ichthyoenteri [MECs $7.4-39.5{\mu}g/ml$] 그리고 진균류인 Candida albicans [MECs $26.0-31.8{\mu}g/ml$]에 항균활성을 나타냈다. 본 연구 결과, 참담치 혈구 유래 mytilin B1과 mytilin B2는 넓은 항균 스펙트럼을 가지고 열과 염분에 대한 안정성이 높으며 용혈현상과 세포독성은 나타나지 않았다. 이러한 특성은 기능성 사료첨가제 및 항생제 대체제로써 충분히 안정적인 역할을 할 뿐만 아니라 추후 mytilin의 구조적 중요성과 참담치의 면역학적 측면에서 다양한 자료를 제시할 것으로 사료된다.

• Asian Journal of Business Environment
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• 제2권1호
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• pp.25-34
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• 2012

It is possible to use pallet for forwarding as chinese cabbages and radishes are general large-scale trading items at the agricultural wholesale market though, however, most of these are forwarded as it have packed in net bags or in P·E bags. Thus, it is still hard for palletizing. The type of packing the product in the net bag makes it difficult for palletizing. It is not a stable shape enough and easily collapsed for pallet loading. Because of this collapsibility, the corrugated cardboard box is being used to enhance forwarding efficiency, but the existing corrugated cardboard box could be crushed easily by moist what is from the agricultural product's property and it also could be squashed by the mass of the loaded box layers on itself. In contrary, the functional waterproof corrugated cardboard box is not collapsed through palletizing and it is efficient for product management with it's ventilation function in respond to pre-cooling effect. Furthermore, because it has various functional shapes as the open type, the partition type and so on, it is effective for maintaining freshness of the product and standardizing the distribution of agricultural product. It is well-known that it is possible to introduce this box to cargo-works of agricultural product. Consequently, the recognition of main distributors about the pallet distribution of the chinese cabbage and the radish was apprehended in this study for activating mechanization of loading and unloading. The survey was conducted to the main distributors such as the forwarder, the auction dealer and the commission merchant with Garak-dong wholesale market as the center. The appropriate packing materials and problems of the existing method for loading and unloading were derived through the survey. Especially, it was focused on analyzing the difference of recognition between the subject groups for the way of using waterproof cardboard corrugated box to deal with the difficult product for packing in normal corrugated box because of the box's absorption of moist from the agricultural product like a chinese cabbage and a radish. Total In the cases of the forwarders and the commission merchants, the net was highly responded as 45%, 74% from each groups for the best packing material for mechanization of distribution and the waterproof corrugated cardboard box was responded as 20%, 22% from each groups as much preferable than multi-stage wooden box. However, for the radish, the waterproof corrugated cardboard box was the best material as 56%, and the auction trader group supported it for 80%. So, the using the waterproof corrugated cardboard box for mechanization of distribution was negative for the chinese cabbage, but it was positive for the radish. The average was 2.42, the standard deviation was 1.24. The negative response(about 55%) was prevailing more than positive response(about 23%). It could be analyzed that even there was the positive recognition for using the waterproof corrugated cardboard box for the radish though the preference for low price of net bag in the chinese cabbage forwarding procedure. Still now, it seems that is a burden for using the waterproof corrugated cardboard box with high price. In the analysis on the recognition differences about using the waterproof corrugated cardboard box for the chinese cabbages and the radish between the forwarders and the commission merchants, generally the negative recognition was prevailing, but the forwarders(2.696) were more positive for using the waterproof corrugated cardboard box than the commission merchants(2.145).

• Restorative Dentistry and Endodontics
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• 제24권1호
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• pp.222-232
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• 1999

The purpose of this study was to evaluate the effect of heated spreader on the sealing ability of lateral condensation, compared with regular cold spreader. Forty two extracted human teeth with single canal were randomly placed into 3 experimental groups, and four additional teeth were used as positive and negative controls. Each group was prepared with Ni-Ti Profile #40 using step-down technique and obturated with standardized colored gutta-percha cone by standard(cold) lateral condensation technique, warm lateral condensation technique with Endotec and hot spreader soaked in glass bead sterilizer, each with Sealapex sealer. Control groups were not obturated, but prepared. After 2 days in 2% methylene blue, the teeth were invested and made into transparent resin blocks. And then, each block was sectioned horizontally with microtome at 1, 2, 3, 4, 5 mm levels from the apex. The linear extent of dye penetration was examined with stereomicroscope at ${\times}$20 magnification. At each of 5 levels, ratio of the area of gutta-percha was obtained by calculating the area of gutta-percha to the total area of the canal. The data collected were then analyzed statistically using an analysis of variance(ANOVA) and Scheffe test. The results were as follows ; 1. All experimental groups produced the apical microleakage. 2. The mean leakage was 1.57${\pm}$0.76mm for cold spreader group, 0.86${\pm}$0.95mm for Endotec spreader group, and 0.64${\pm}$0.93mm for hot spreader group. The difference between hot spreader group and cold spreader group was statistically significant(p<0.05). 1. At the 1 mm level, the mean ratio of area of gutta-percha was 74.58${\pm}$13.15(%) for cold spreader group, 65.42${\pm}$14.62(%) for Endotec spreader group, and 80.72${\pm}$14.63(%) for hot spreader group. There was statistically significant difference between hot spreader group and Endotec spreader group(p<0.05). 2. At the 2mm level, the mean ratio of area of gutta-percha was 87.86${\pm}$11.22(%) for cold spreader group, 66.55${\pm}$14.02(%) for Endotec spreader group, and 92.93${\pm}$7.24(%) for hot spreader group. There was statistically significant difference between Endotec spreader group and other two spreader groups(p<0.05). 3. At the level 3, 4, 5 mm, there was no statistically significant difference between each group. Within the limits of the results of this experiment, warm lateral condensation technique with hot spreader soaked in a glass bead sterilizer demonstrated favorable apical sealing effect and improved density of gutta-percha mass. Thus, it is thought that this obturation technique is effective for clinical use and beneficial to reduce condensation forces, also economical and easy. Lateral condensation, Heated spreader, canal sealing, Microleakage.

• 한국식품위생안전성학회지
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• 제32권3호
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• pp.193-198
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• 2017

본 연구는 건강기능식품 중 baicalin, eleutheroside E, ligustilide를 효과적으로 분석할 수 있는 방법을 확립하기 위하여 수행되었다. 이에 LC-MS/MS를 이용하여 신속하고 효율적으로 동시분석할 수 있는 시험법을 확립하였으며, 확립된 시험법에 대해 특이성, 검출한계, 정량한계, 정확도, 정밀도에 대한 검증을 통하여 유효성을 확인하고자 하였다. 표준용액을 이용하여 검량선을 작성한 결과 $r^2$ > 0.99 이상의 직선성을 확인하였고, baicalin, eleutheroside E, ligustilide에 대한 정량한계는 각각 $39.3{\mu}g/L$, $106.7{\mu}g/L$, $76.1{\mu}g/L$이었으며, 검출한계는 각각 $13.0{\mu}g/L$, $35.2{\mu}g/L$, $25.1{\mu}g/L$이었다. 또한 평균 회수율은 각 성분에 대해 108.0~109.9%, 99.8~101.3%, 91.4~97.2%로 나타났으며, 반복정밀도는 상대표준편차 5%이하, 실험실간 재현성은 9%이하로 나타나 정확성, 재현성이 우수하였으며 이는 AOAC 가이드라인에서 제시한 기준에 모두 적합한 수준이었다. 따라서 개발된 분석법은 향후 건강기능식품 중 baicalin, eleutheroside E, ligustilide를 동시분석하는데 효과적으로 활용할 수 있을 것으로 판단된다.

• 한국식품영양학회:학술대회논문집
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• 한국식품영양학회 2003년도 하계 학술 심포지엄
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• pp.76-76
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• 2003

This study was conducted to investigate dietary and other factors affecting bone mineral density (BMD) in Korean premenopausal women. Seventy-eight premenopausal women who visited health promotion center for health examinations volunteered to participate in this study and they were divided into two groups according to the bone status by T-score : normal or osteopenic group and osteoporotic group. The demographic and general characteristics, and dietary intake were surveyed using the questionnaire. BMDs of the lumbar spine and femoral neck of subjects were measured by dual energy X-ray absorptiometry. Serum samples were measured for Lipid concentration, and calcium, phosphorus, alkaline phosphatase as bone formation indicators. Urine was analysed for creatinine as bone resolution indicators. The results are as follows:The mean BMDs of the lumbar spine and femoral neck were 1.21 0.02g/cm$^2$ and 0.97 0.04g/cm$^2$, respectively and the BMD levels of osteoporotic group were significantly lower than that of normal or osteopenic group (p<0.001, respectively). Height in osteoporotic group was significantly lower than that of normal or osteopenic group (p<0.01), and bodyweight did not show any significant difference but had a lower tendency. Mean daily intake of energy was 1720 52ka1. When nutrient intake was compared with recommended dietary allowances (RDA) of subjects, calcium, Fe, vitamin A and riboflavin intakes showed means lower than RDA. The nutrient intake did not show any significant difference between normal or osteopenic group and osteoporotic group except intakes of protein fat and niacin. Serum and urine levels did not show any significant differences between normal or osteopenic group and osteoporotic group and all were within normal range, however, serum alkaline phosphatase level of osteoporotic group was significantly higher than that of normal or osteopenic group (p<0.001). Height showed positive correlations with lumbar spine bone mineral density (LBMD, r=0.332, p<0.01), no correlation was found with femoral neck bone mineral density (NBMD). Age, age at menarche, bodyweight, body mass index (BMI) and obesity showed no correlation with BMD. The BMD of the lumbar spine was significantly and positively related to the intake of niacin and vitamin C (r=0.236, p<0.05; r=0.274, p<0.05). Serum levels of calcium and phosphorus showed a negative correlation with LBMD (r=-0.698, p=0.0001; r=-0.503, p=0.0001, respectively). The results suggested that the BMDs of the lumbar spine was positively related to the intake of niacin and vitamin C in premenopausal women. Therefore, this study confirmed that one of the most effective way to minimize bone loss would be higher intake of niacin and vitamin C rich foods and habitual physical activity may have a beneficial effect on BMD in premenopausal period.

• 한국융합학회논문지
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• 제3권1호
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• pp.27-34
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• 2012

2011년 3월 11일 발생한 일본 후쿠시마 원전 사고 후 한국 대학생들의 방사선과 의료 방사선에 대한 생각을 파악하여 방사선에 대한 적절한 교육 방법과 국민들의 방사선 안전에 대한 올바른 대중 커뮤니케이션에 대해 모색하고자 전국 5개도(경기, 강원, 충청, 전라, 경상도)의 4년제 방사선학과가 설치된 대학교에서 790부 설문지를 수거한 후 분석하였다. 설문은 36문항으로 구성하였으며, '방사선이 위험하다'고 느끼는 중요 원인 중 하나는 '적은양의 방사선을 쪼여도 나중에 문제가 생길 수 있다'. ($3.28{\pm}1.05$)로 분석됐다. 방사선에 대한 관리에서는 개인보다는 정부가 나서야 된다는 응답자가 $3.87{\pm}0.89$으로 나타났다. '후쿠시마 원전 사고는 원자력 발전을 하지 말아야 한다는 쪽으로 나의 생각을 바꾸게 했다'라는 문항에는 $2.79{\pm}0.95$로 나타났다. '후쿠시마 원전사고 이후 의료 방사선에 대한 생각이 부정적으로 바뀌었다'라는 문항에는 전체적으로 $2.64{\pm}1.02$, 방사선학과 $2.31{\pm}1.00$, 치위생학과 $2.94{\pm}0.95$, 기타학과 $3.13{\pm}0.82$로 방사선과 관련된 교과목을 수강 경험이 없는 기타학과 학생일수록 부정적인 생각을 가지고 있는 것으로 나타났다. 또한 방사선에 관한 교육이 필요하다고 생각하는 사람이 82.28%로 나타났으며, 그 중 T.V나 인터넷이 가장 효율적인 교육 방법으로 나타났다.