• Food Science and Biotechnology
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• v.17 no.5
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• pp.912-918
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• 2008

This study was conducted to identify the differences in proteomic characteristics of 'Agakong', recombinant inbred line, and its parental genotypes 'Eunhakong' (Glycine max) and 'KLG10084' (G. soja). The isoflavone content of 'Agakong' was 3 times higher than that of its parental lines. A combined high-throughput proteomic approach was employed to determine the expression profile and identity of proteins using 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. The overall distribution patterns of proteins are quite similar, but lots of protein spot intensities varied among the genotypes. A total of 41 proteins, representing significant difference in the quantities of protein among the lines, were successfully identified. Among them, more than 50% of the proteins identified were subunits of glycinin and $\beta$-conglycinin, 2 major storage proteins. This study showed that the proteomic analysis could help to define specific changes in protein level and composition, which can occur in the generation of new soybean varieties.

• Molecules and Cells
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• v.20 no.3
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• pp.354-360
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• 2005

Neuronal damage subsequent to transient cerebral ischemia is a multifactorial process involving several overlapping mechanisms. Gangliosides, sialic acid-conjugated glycosphingolipids, reduce the severity of acute brain damage in vitro. However their in vivo effects on the cerebral cortex damaged by ischemic infarct are unknown. To assess the possible protective role of gangliosides we examined their expression in the cerebral cortex damaged by ischemic infarct in the rat. Ischemia was induced by middle cerebral artery (MCA) occlusion, and the resulting damage was observed by staining with 2, 3, 5-triphenylterazolium chloride (TTC). High-performance thin-layer chromatography (HPTLC) showed that gangliosides GM3 and GM1 increased in the damaged cerebral cortex, and immunofluorescence microscopy also revealed a significant change in expression of GM1. In addition, in situ hybridization demonstrated an increase in the mRNA for ganglioside GM3 synthase. These results suggest that gangliosides GM1 and GM3 may be synthesized in vivo to protect the cerebral cortex from ischemic damage.

• Journal of Korean Association for Spatial Structures
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• v.16 no.2
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• pp.47-54
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• 2016

It is very important to calculate natural frequency of the observatory tower correctly because it is keenly affected by wind response vibration due to its large slenderness ratio, weight and small damping ratio. Additionally, suggestion equation of natural frequency being used in the design phase has considerable difference between actual measured value thereby making it inappropriate to be used in the serviceability design of the observatory tower. Therefore, this paper conducted an ambient vibration measuring on 10 observatory towers through mobile-phone application thereby calculating the natural frequency and comparing the result with the domestic and foreign standards and that of the eigen-value analysis. This paper suggested approximate equation of the natural frequency of the observatory tower; T=0.0266H. The square of the corelation coefficient is 0.940, which is high.

• Journal of The Korean Association For Science Education
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• v.27 no.1
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• pp.9-17
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• 2007

This study sets out to assess the scientific literacy of secondary school students and to describe their differences according to gender, grade, course. This study involved 112 middle school students and 213 high school students. Their scientific literacy was measured by the Scientific Literacy Test designed by Manhart (1997). A 70-item multiple-choice test was used to assess their scientific literacy. The constructs of science factor included 36 items making up physical science, life science, and earth science subtests. The social aspects of science factor consisted of 34 items in nature of scientific inquiry/knowledge, science as a human endeavor, science and technology, and societal perspectives. A two-way analysis of variance (ANOVA) and t-test were conducted using the SPSS program. The scientific literacy score of the middle school students was 45.17. There was no significant difference according to gender but boys tended to perform better than girls on both the constructs of science factor and the social aspects of science factor. The scientific literacy score of the high school students was 51.79. There was no significant difference according to gender. But, boys tended to perform better than girls on the constructs of science factor. Girls tended to perform better than boys on the social aspects of science factor. The students taking a course on natural science got statistically higher scores than the students taking a course on humanities. The high school students got statistically higher scores than the middle school students.

• Journal of applied mathematics & informatics
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• v.36 no.1_2
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• pp.69-79
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• 2018

The Euclid metric is well-known and there are many results on the space with that metric. But there are many other metrics which gives more practical and useful results in the plane. In this paper, we introduce new metric function in the plane, which is more useful in city with subway. Finally we generalize to the general metric space and introduce a new metric on ${\mathbb{R}}^n$.

• Korean Parent-Child Health Journal
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• v.14 no.2
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• pp.62-68
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• 2011

Purpose: This study was performed to investigate contraception knowledge and sexual behavior in female high school students. Methods: The participants were 382 female high school students from one academic and one vocational school. Data was collected from October to November, 2008 using self report questionnaire. Results: Knowledge on contraception was 5.52 in maximum 14 point scale. Major information source for sexual matters was peers such as friends or sisters. Perception on sexual behavior was different according to major information source. Fifty five percents of students thought that kiss can be permitted, and regarded sexual behavior as natural development. 3.4% of participants had experience of sexual intercourse. Conclusion: It is indicated that perception on sexual behavior can be shaped according to correct information and positive attitude on natural sexual development by health profession. Also, education on contraception would be included in sex education to reduce teenage pregnancy.

• Journal of the Korean Society of Earth Science Education
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• v.8 no.3
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• pp.332-345
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• 2015

Earth science is the study to explore the planet in which we live. Among these earth science geology of the area it can be the most critical and important study. However, because of the size and scope is too broad temporal spatial eurona covered in geology is true that many students find difficult about the geology field. In this study, in conjunction with landscape formation of geologic time for the concept to be among the core areas of Geology examined the concept and recognize it as the destination for high school students. Is a test tool for the analysis was adapted for use by Jolley (2010) has developed LIFT (The Landscape Identification and Formation Test). Currently we fix the strip to match the country through a validity check of the curriculum. Results of the study were as follows: First, the ability to check the landscape and formation is expected to estimate the time and the liberal arts students was higher than the natural science students. The reason for this seems to be the influence of learning geographical subjects. Second, the concept of geological time was found to lack both natural science and liberal arts students. The reason is that the students in the previous process because it deals with the concept of geologic time from the top of Earth Science Education II seems to be because there was no chance of learning about geological time. Third, the results confirm the confidence of the students surveyed in the landscape formation time natural science students was higher than liberal arts students. The research measured gender boys higher than girls. Fourth, the students on the landscape and geological time was found to have a number of misconceptions. This appears to be due to the students to feel difficulty in thinking of the concept because the need to understand the abstract geologic time. Therefore, it is necessary just to hold misconceptions about the concept of geology students have through the study of the landscape and geological time.

• Journal of the Korean Society of Earth Science Education
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• v.12 no.3
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• pp.291-301
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• 2019

The purpose of this study is to develop HTE-STEAM program using natural disasters for high school students and to verify their attitude and satisfaction with STEAM. We developed the HTE-STEAM program utilizing natural disasters and tested the effectiveness of 243 students from G High School and N High School. For data analysis, a single group pretest and post response t-test were conducted to verify the effects on attitudes and satisfaction with STEAM. The research results are as follows. First, we developed STEAM education programs for high school students by selecting the topic of "natural disasters" in the area of earth science and flying drones as lifesaving drones. The six-hour program was designed to give students the experience to solve problems by applying essential knowledge related to natural disasters and drones, and what they learned in other situations. Second, there was a significant statistical test result in the t-test of the corresponding sample by the difference between the pre and post score of the STEAM attitude test (p <.05). The drone-based HTE-STEAM program had a significant improvement in the overall attitude toward STEAM education, which consists of seven subfactors. Third, in the HTE-STEAM satisfaction test, the average value of the lower job offer was 3.64 ~ 3.76, which showed a positive response overall. It is judged that the students' satisfaction is improved through the students' understanding of the problem situation and the design of creative convergence and production process.

• Molecules and Cells
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• v.23 no.1
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• pp.17-22
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• 2007

We have expressed human erythropoietin (EPO) in transgenic mice using a recombinant EPO cDNA combined with a partial TPO construct. The gene was microinjected using standard techniques and five mice were detected as transgenic by PCR and further used as founders. The life span of the transgenic founders was much shorter than that of their normal littermates. Most of the tissues of the transgenic founders contained human EPO transcripts as judged by RT-PCR. Especially high expression levels were seen in the liver and lung. EPO protein levels in serum were examined by ELISA and ranged from 266-414 mIU/ml. The number of red blood cell, white blood cell and hemoglobin in the hEPO transgenic mice was higher than in normal mice. These results indicate that overexpression of hEPO is deleterious and can provoke lung failure and erythrocytosis.

• Microbiology and Biotechnology Letters
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• v.35 no.3
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• pp.255-260
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• 2007

This study was conducted to isolate and identify bacteria producing xylanase and cellulase from spent mushroom substrates and to determine the optimal medium conditions for their growth. Bacteria showing high xylanase and carboxymethyl cellulase activities and low protease and amylase activities were strain 201-3 and strain 206-3. Strain 201-3 was identified as Enterobacter ludwigii and named Ent. ludwigii KU201-3. 206-3 was identified as Bacillus cereus and named B. cereus KU206-3. The optimal medium condition of Ent. ludwigii KU201-3 was obtained when 1%(w/v) of soybean meal and 3%(w/v) of sucrose were used as nitrogen and carbon source, respectively. That of B. cereus KU206-3 was obtained when 3%(w/v) of soybean meal and 1%(w/v) of molasses were used as nitrogen and carbon sources, respectively.