Browse > Article

Isolation and Identification of High Cellulolytic Bacteria from Spent Mushroom Substrate and Determination of Optimal Medium Conditions for the Growth  

Kim, Young-Il (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
Jung, Se-Hyung (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
Seok, Joon-San (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
Yang, Si-Yong (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
Huh, Jeong-Weon (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
Kwak, Wan-Sup (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
Publication Information
Microbiology and Biotechnology Letters / v.35, no.3, 2007 , pp. 255-260 More about this Journal
Abstract
This study was conducted to isolate and identify bacteria producing xylanase and cellulase from spent mushroom substrates and to determine the optimal medium conditions for their growth. Bacteria showing high xylanase and carboxymethyl cellulase activities and low protease and amylase activities were strain 201-3 and strain 206-3. Strain 201-3 was identified as Enterobacter ludwigii and named Ent. ludwigii KU201-3. 206-3 was identified as Bacillus cereus and named B. cereus KU206-3. The optimal medium condition of Ent. ludwigii KU201-3 was obtained when 1%(w/v) of soybean meal and 3%(w/v) of sucrose were used as nitrogen and carbon source, respectively. That of B. cereus KU206-3 was obtained when 3%(w/v) of soybean meal and 1%(w/v) of molasses were used as nitrogen and carbon sources, respectively.
Keywords
Xylanase; CMCase; Mshroom; Enzyme activity; By-product;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Ha, J. K., S. S. Lee, Y. S. Moon and C. H. Kim. 2005. Ruminant nutrition and physiology. Seoul National Univ. Press
2 Thompson, J. D., D. Q Higgins, and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalies and weight matrix choice. Nucleic Acids Res. 22, 4673-4680   DOI
3 Yang, S. Y., M. D. Song, O, H. Kim, and C. W. Kim. 2001. Isolation of Bacillus sp. producing multi-enzyme and optimization of medium conditions for its production Using Feedstuffs for Probiotics. Kor. J. Appl. Microbiol. Biotechnol. 29: 110-114
4 Kim, Y. I., J. S. Bae, J. W. Huh and W. S. Kwak. 2007. Monitoring of feed-nutritional components, toxic heavy metals and pesticide residues in mushroom substrates according to bottle type and vinyl bag type cultivation. Kor. J. of Ani. Sci. 49: 67-78   DOI
5 Tuomela, M., M. Vikman, A. Hatakka and M. Itavaara. 2000. Biodegradation of lignin in a compost enviroment:a review. Bioresource Tech. 72: 169-183   DOI   ScienceOn
6 Ha, H. C. 2001. Purification and characterization of manganese peroxidase from Pleurotus ostreatus. Proc. Asian Mycol. Symp.209-214
7 Makela, M., S. Galkin, A. Hatakka and T. Lundell. 2002. Production of organic acids and oxalate decarboxylase in lignin-degrading white rot fungi. Enzyme and Microbial Technology 30: 542-549   DOI   ScienceOn
8 Campbell, G. L. and M. R. Bedford. 1992. Enzyme applications for monogastric feeds: A rewiew. Can. J. Anim. Sci. 72: 449   DOI
9 Khasin, A., I. Alchanati and Y. Shoham. 1993. Purification and characterization of a thermostable xylanase from Bacilus stearothermophilus T-6. Appl. Environ. Microbiol. 59: 1725-1730   PUBMED
10 Morohoshi, N., T. Nakamura and T. Haraguchi. 1985. Degradation of lignin by the extracellular enzymes of Tramates versicolor(I). Tokyo Univ. of Agri. and Tech. 21: 101- 105
11 Jung, W. H., S. Y. Yang, M. D. Song, J, K. Ha and C. W. Kim. 2003. Isolaton of Bacillus sp. producing xylanase and cellulase and optimization of medium conditions of its production. Kor. J. Appl. Microbiol. Biotechnol. 31: 383-388
12 Claus, D. and R. C. W. Berkeley. 1986. Genus Bacillus, PP1105-1139. In Sneath, P. H. A. (ed), Bergey's Manual of Systematic Bacteriology, Vol. 2, Williams and Wilkins, Baltimore, MD
13 Miller, L. T. 1982. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters. including hydroxy acid. J. Clin. Microbiol. 18: 861-867
14 Miller, G, L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428   DOI