• Title/Summary/Keyword: natural mixed culture

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Isolation and Culture Characteristics of Strains for Color Removal of Disperse Dyes (분산성 염료의 색도제거를 위한 균주의 분리 및 성장 특성)

  • 조무환;허만우;한명호;강건우
    • Textile Coloration and Finishing
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    • v.12 no.1
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    • pp.25-31
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    • 2000
  • In order to decolorize disperse dyes by using biological treatment process, a strain which has potential ability to degrade disperse dyes was isolated from natural system. To increase the removal efficiency of decolorization in the aqueous solutions, the optimal condition of decolorization by this strain was investigated, and continuous plant test was also developed. The optimal culture conditions of temperature and pH were found to be 4$0^{\circ}C$ and 8.5~9, respectively. When yeast extract was mixed with polypeptone at the mixing ratio of 1:1 as a nitrogen source, decolorization efficiency was highest(93%) among the nitrogen sources. The strain to be screened was excellent to adjust to pH, and it seems to be have ability to control pH needed to growth. The optimal culture conditions in concentration of $MgSO_4\cdot{7H}_2O$ and $KH_2PO_4$ were 0.1%(w/v) and 0.2%(w/v). The result of continuous plant process using wastewater was as following : $COD_{Mn}$ removal efficiency was over than 50%, and this strain was very excellent in decolorization-efficiency for the wastewater of Taegu dyeing complex.

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Overcoming Encouragement of Dragon Fruit Plant (Hylocereus undatus) against Stem Brown Spot Disease Caused by Neoscytalidium dimidiatum Using Bacillus subtilis Combined with Sodium Bicarbonate

  • Ratanaprom, Sanan;Nakkanong, Korakot;Nualsri, Charassri;Jiwanit, Palakrit;Rongsawat, Thanyakorn;Woraathakorn, Natthakorn
    • The Plant Pathology Journal
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    • v.37 no.3
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    • pp.205-214
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    • 2021
  • The use of the supernatant from a Bacillus subtilis culture mixed with sodium bicarbonate was explored as a means of controlling stem brown spot disease in dragon fruit plants. In in vitro experiments, the B. subtilis supernatant used with sodium bicarbonate showed a strong inhibition effect on the growth of the fungus, Neoscytalidium dimidiatum, the agent causing stem brown spot disease and was notably effective in preventing fungal invasion of dragon fruit plant. This combination not only directly suppressed the growth of N. dimidiatum, but also indirectly affected the development of the disease by eliciting the dragon-fruit plant's defense response. Substantial levels of the pathogenesis-related proteins, chitinase and glucanase, and the phenylpropanoid biosynthetic pathway enzymes, peroxidase and phenyl alanine ammonia-lyase, were triggered. Significant lignin deposition was also detected in treated cladodes of injured dragon fruit plants in in vivo experiments. In summary, B. subtilis supernatant combined with sodium bicarbonate protected dragon fruit plant loss through stem brown spot disease during plant development in the field through pathogenic fungal inhibition and the induction of defense response mechanisms.

Introduction of Asite in Japan (위수개론)

  • 전용범
    • Archives of design research
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    • v.14 no.4
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    • pp.127-136
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    • 2001
  • The decay of the Old Chinese Dynasty, Tang in the 10th century caused the collapse of the political order of Southeast Asia and the neighboring countries began to try to go their own way. Japan abolished the system called "Yudangsa" and nourished the culture of Japanese style. The fang age had a respect of the′Japanese Character Culture", in which when we expressed the sound, they developed the Japanese letters ,that is, reduced Chinese characters, and systematized them as linguistic symbols and developed them into one of letter design styles. The Japanese letters which was mainly the media of the expression of the female literature were delicately shaped ′as natural materials, which finally resulted in Japan′s oldest letter modeling, Asite Letter Design Style. The Japanese letters were blended with ′Reeds around the Lake", "Sea Birds", "Birds′flying", and "Rocks", mixed with the overall shapes of the objects and assimilated into one part, and became a means for the main entertainment of the nobility such as "Singing Festival", "Conference", and "Ceremonial Events" According to the relics, the Japanese feeling of a small cosmos was represented with the fabulous skills of artifacts. In audition, "Play Painting"in the countryside is also a kind of Asite the ordinary people accepted as wit and satire. What we will have to notice is about exchange of the Chinese letter designs, when the thought focused ′on the letter design, Jobcheso was popular. The letter design Jobcheso is -a kind of letter design which represents -natural objects and was popular at the time of Yukjo Dynasty, and there are some documents of that period left both in Japan and Korea. "Hyojedo" in Korea is supposed to have been influenced by the letter design. Asite- is also considered to have been "Japanese Letter Jobcheso." Therefore, the purpose of this study is to look into the origin of the letter designs in the Chinese character culture circle, in terms of the letter design of Jobcheso.

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Design expression method of the sexual image of evening dresses shown in the haute couture collection (오뜨꾸뛰르 컬렉션에 나타난 섹슈얼 이미지 이브닝 드레스 디자인 표현방법)

  • Peng, Xiaochun;Eum, Jungsun;Yoo, Youngsun
    • The Research Journal of the Costume Culture
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    • v.24 no.5
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    • pp.642-652
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    • 2016
  • The purpose of this study is to understand the concept of a sexual image and verify the method of its design expression through case studies of the sexual image evening dress shown in an Haute Couture Collection over the last 10 years (2005~2014). The results of analysis are as follows: First, "expression by the seeing through of the natural body" expressed a natural sexual image that combined fashion of the previous times with the beauty of the natural body by using a see-through material and classic or ethnic image. Second, "expression of a sexual-image look that emphasizes romantic detail" illustrated a romantic sexual image that emphasizes feminine sensitivity by mixing various ornamental elements such as see-through material and symbolism of underwear image. Third, "creation of a nude look using skin color" expressed sexual image of an evening dress by inducing erotic sexual association through a combination of opaque material of skin color and design shape that is intended for body exposure. Fourth, "combination of sexual symbol and heterogeneous elements" expressed a decadent, avant-garde and futuristic sexual images by using women's underwear and a sexual symbol as design motif and mixed with mismatching elements. Fifth, "use of a fantastic black image" expressed an exclusive and refined sexual image and a decadent and primitive sexual image by using a fantastic image of black color. The results of this study are expected to be used for the design process of the evening-dress industry that aims for quality improvement.

Aerobic Liquid Fermentation of Food Wastes by Using Yeast (효모에 의한 남은 음식물의 호기성 액상발효)

  • Lee, Ki-Young;Yu, Sung Jin;Yu, Seung Yeung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.8 no.4
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    • pp.147-152
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    • 2000
  • For the probiotic feed production from residual food waste by using the yeast Kluyveromyces marxianus, aerobic liquid fermentation was attempted at $35^{\circ}C$. After grinding finely, optimal fermentation conditions of the substrate was investigated in shaking incubator. By controlling water content yeast growth was studied at each different solid content of 5, 10 and 15% respectively. The most active growth of the yeast was shown at 10%. For the stimulation of the cell growth, mixed culture with Aspersillus oryzae was conducted in a 2 litre-jar fermenter. As the results, the yeast growth rate was increased, but the maximum viable cell count amounted was slightly higher as $3.5{\times}10^9/ml$ than single culture.

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Sensuous Characteristics and Physiological Activity of Cheongtaejeon Tea Produced with the Inoculation of Microbial Strains (균주를 접종하여 제조한 청태전 차의 관능적 특성과 생리활성 효과)

  • Heo, Buk-Gu;Cho, Jung-Il;Park, Yong-Seo;Park, Yun-Jum;Cho, Ja-Yong
    • The Korean Journal of Community Living Science
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    • v.21 no.1
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    • pp.139-148
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    • 2010
  • This study was conducted to restore our traditional Cheongtaejeon tea and to develop the special products. We inoculated Cheongtaejeon tea with lactobacilli (Lactobacillus plantarum CHO 25) and the mixed microbial strains (L. plantarum CHO 25 + Saccharomyces cerevisiae + Bacillus amyloliquefaciens CHO 104). We also examined the sensuous characteristics and physiological activity of Cheongtaejeon tea which was produced by the inoculation of microbial strains. The external appearance of Cheongtaejeon teas were not significant among the teas which were produced with or without the inoculation of L. plantarum CHO 25 and the mixed microbial strains. The taste of the tea increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains. The taste and liking of Cheongtaejeon tea which was inoculated with Aspergillus oryzae and Aspergillus niger decreased significantly, and it was not suitable to drink. Total phenolics compound contents, total flavonoid contents and DPPH ($\alpha,\alpha$-diphenyl-$\beta$-picryl-hydrazyl) radical scavenging activity of Cheongtaejeon tea extracts increased much more in the order of that produced with the inoculation of L. plantarum CHO 25, control and that with the mixed microbial strains. However, nitrite radical scavenging activity in 1,000 mg/L Cheongtaejeon tea hot water extracts were in the order of the control (94.4%), the inoculation of L. plantarum CHO 25 (93.6%) and the mixed microbial strains (91.1%). Overall results indicated that the sensuous characteristics increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains, and those physiological activities in tea with the inoculation of L. plantarum CHO 25.

Cell-laden Gelatin Fiber Contained Calcium Phosphate Biomaterials as a Stem Cell Delivery Vehicle for Bone Repair (세포 함유 젤라틴 파이버 응용을 통한 골 재생 유도용 인산칼슘 생체재료 세포 탑재 연구)

  • Kim, Seon-Hwa;Hwang, Changmo;Park, Sang-Hyug
    • Journal of Biomedical Engineering Research
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    • v.43 no.1
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    • pp.61-70
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    • 2022
  • Natural and synthetic forms of calcium phosphate cement (CPC) have been widely used in bone repair and augmentation. The major challenge of injectable CPC is to deliver the cells without cell death in order to regenerate new bone. The study objective was to investigate for the potential of stem cell-laden gelatin fibers containing injectable, nanocrystalline CPC to function as a delivery system. Gelatin noddle fiber method was developed to delivered cells into nCPC. Experimental groups were prepared by mixing cells with nCPC, mixing cell-laden gelatin fibers with nCPC and mixing cell-laden gelatin fibers containing BMP-2 with nCPC. Media diffusion test was conducted after dissolving the gelatin fibers. SEM examined the generated channels and delivered cell morphology. Fibers mixed with nCPC showed physical setting and hardening within 20 min after injection and showed good shape maintenances. The gelatin fibers mixed nCPC group had several vacant channels generated from the dissolved gelatin. Particularly, proliferation and attachment of the cells were observed inside of the channels. While live cells were not observed in the cell mixed nCPC group, cells delivered with the gelatin fibers into the nCPC showed good viability and increased DNA content with culture. Cell-laden gelatin fiber was a novel method for cell delivery into nCPC without cell damages. Results also indicated the osteogenic differentiation of gelatin fiber delivered cells. We suggest that the cell-laden gelatin fibers mixed with nCPC can be used as an injectable cell delivery vehicle and the addition of BMP-2 to enhances osteogenesis.

Galician Nature and People as Seen through La hija del mar by Rosalía de Castro (로살리아 데 카스트로의 『바다의 딸』에 나타난 갈리시아의 자연과 인간)

  • Song, Sun-ki
    • Cross-Cultural Studies
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    • v.41
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    • pp.177-197
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    • 2015
  • This study found that La hija del mar (1859) by Rosalía de Castro describes, as the background to the work, the lives of Galicians, along with the movement of the sea, scenes of the coast, storms, and lively plants in Muxía, a small city of Galicia and Cabo de Finisterre. It was shown that the nature of Galicia, as described in the work, is a beautiful site where fishermen and farmers live among the full aroma of plants and flowers, as well as being a subject of fear to the people there. Furthermore, it was also shown through the funeral process, along with a depiction of the natural environment, the unique place of Galicia is part of the traditional heritage that the Galician people have preserved, in which Roman Catholic rituals are mixed with superstitious rites. Moreover, the author shows through a description of the excessive drinking culture of Dionysian rites or Libation rites that Galicia is a land with longstanding and profound folk customs, as well as a special culture. It was proved that the work is a specific representation of the author's perspective. Thus, Rosalía depicts the lives of people and the natural environment, which have not been examined profoundly, although they always existed in Galicia. This provides space where the external reality of Galicia and part of the identity of Galicia can be elucidated.

An Evaluation of Rural Landscape and Comparative Analysis in Accordance with Space Types : Focused on Residents and Visitors of Seondong Region, Gochang-Gun, Jeollabuk-Do, Korea (공간유형별 농촌경관 평가 및 비교 분석 - 전북 고창 선동권역의 주민과 방문객을 대상으로 -)

  • Baek, Jong-In;Ban, Yong-Un;Woo, Hye-Mi;Choi, Na-Rae
    • Journal of Korean Society of Rural Planning
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    • v.16 no.4
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    • pp.1-11
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    • 2010
  • This study has intended to evaluate rural subjective landscape through participation of residents and visitors according to space types, and to perform comparative analysis of evaluation results between residents and visitors. This study has employed a survey method for which 58 residents of 8 villages within Seondong region at Gochang-gun and 70 visitors to Green Barley Field Festival in the target region have participated. 42 landscape view points were selected according to landscape scopes and space types, and then the preference was evaluated using landscape adjectives after showing pictures already taken for each landscape view point. This study has found the following results. First, whereas residents gave high points to natural landscape and artificial one at the historical culture areas in comparison with other landscape scopes, visitors gave them low points on the other hand. Second, visitors evaluated the cultivated area among space types of mixed landscapes with high value. Third, based on t-test for comparative analysis, the statistically significant differences of evaluation results appeared at 6 places among 8 natural landscapes, 3 places among 12 artificial landscapes, and 3 places among mixed landscapes.

Viral Infection of Tissue Cultured Orchids and Evaluation of Damages

  • Chung, Bong-Nam;Yoon, Ju-Yeon;Kim, Mi-Sun
    • The Plant Pathology Journal
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    • v.26 no.2
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    • pp.194-197
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    • 2010
  • Most orchids are propagated by tissue culture. To survey the viral infection of tissue cultured Orchids, total RNA was extracted from in vitro Cymbridium and Phalaenopsis spp. collected from companies producing tissue-cultured orchids, and RT-PCR analysis was conducted with primer pairs specific to Cymbidium mosaic virus (CymMV) and Odontoglossum ring spot virus(ORSV), which are infecting wide range of orchid genera. The bulb size of Cymbidium infected with CymMV and ORSV was compared with healthy one at 10 months after planting in vitro orchids in the glasshouse. The CymMV or ORSV infection in 97 Cymbidium and 55 Phalaenopsis plants was 84.5 and 89.1 %, respectively. Mixed infection was found in 52.6 and 47.3% of Cymbidium and Phalaenopsis tested, whereas virus-free orchids were 15.5 and 10.9%, respectively. The CymMV and ORSV reduced the bulb size by 2.7-50% depending on the cultivars of Cymbidium. The both viruses caused yellowing, mottle and mosaic with or without necrosis in 4 Cymbidium cultivars.