[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5423/PPJ.2010.26.2.194

Viral Infection of Tissue Cultured Orchids and Evaluation of Damages

Chung, Bong-Nam (National Institute of Horticultural and Herbal Science, Rural Development Administration)
Yoon, Ju-Yeon (Institute of Natural Science, Seoul Womens University)
Kim, Mi-Sun (National Institute of Horticultural and Herbal Science, Rural Development Administration)

Publication Information

The Plant Pathology Journal / v.26, no.2, 2010 , pp. 194-197 More about this Journal

Abstract

Most orchids are propagated by tissue culture. To survey the viral infection of tissue cultured Orchids, total RNA was extracted from in vitro Cymbridium and Phalaenopsis spp. collected from companies producing tissue-cultured orchids, and RT-PCR analysis was conducted with primer pairs specific to Cymbidium mosaic virus (CymMV) and Odontoglossum ring spot virus(ORSV), which are infecting wide range of orchid genera. The bulb size of Cymbidium infected with CymMV and ORSV was compared with healthy one at 10 months after planting in vitro orchids in the glasshouse. The CymMV or ORSV infection in 97 Cymbidium and 55 Phalaenopsis plants was 84.5 and 89.1 %, respectively. Mixed infection was found in 52.6 and 47.3% of Cymbidium and Phalaenopsis tested, whereas virus-free orchids were 15.5 and 10.9%, respectively. The CymMV and ORSV reduced the bulb size by 2.7-50% depending on the cultivars of Cymbidium. The both viruses caused yellowing, mottle and mosaic with or without necrosis in 4 Cymbidium cultivars.

Keywords

CymMV; ORSV; RT-PCR; tissue culture; virus;

Citations & Related Records

Times Cited By Web Of Science : 0 (Related Records In Web of Science)

Reference

1	Chang, M. U., Chun, H. H., Baek, D. H. and Chung, J. D. 1991. Studies on the viruses in orchids in Korea. 2. Dendrobium mosaic virus, Odontoglossum ringspot virus, Orchid fleck virus, and unidentified potyvirus. Korean J. Plant Pathol. 7:118-129.
2	Homma, Y. and Asahira. T. 1985. New means of Phalaenopsis propagation with internodal sections of flower stalks. J. Japan. Soc. Hort. Sci. 54:379-387. DOI
3	Ishii, M. 1974. Partial elimination of virus from doubly infected orchids by meristem explants culture. Acta Hort. 36:229-234.
4	Kassanis, B. 1965. Therapy of virus infected plants. J. R. Agric. Soc. Engl. 126:105-114.
5	Khentry, Y., Paradornuwat, A., Tantiwiwat, S., Phansiri, S and Thaveechai, N. 2006. Incidence of Cymbidium mosaic virus and Odontoglossum ringspot virus in Dendrobium spp. in Thailand. Crop Protect. 25: 926-932. DOI ScienceOn
6	Lawson, R. H. and Hsu, H. T. 1995. Orchid. In: Virus and Viruslike Diseases of Bulb and Flower Crops. Ed. by G. Loebenstein, R. H. Lawwon and A. A. Brunt, pp. 409-420, John Wiley & Sons, N. Y.
7	Park, W. M., Shim, K. B., Kim, S. J. and Ryu, K. H. 1998. Detection of Cymbidium mosaic virus and Odontolossum Ringspot virus by ELISA and RT-PCR from cultivated orchids in Korea. Korean J. Plant Pathol. 14:130-135.
8	Ryu, K. H., Kim, S. J. and Park, W. M. 1998. Incidence of cymbidium mosaic virus and odontoglossum ringspot virus in Cymbidium and other orchids in Korea. J. Kor. Soc. Hort. Sci. 39:213-217.
9	Sheffield, F. M. L. 1942. Presence of virus in the primordial meristem. Annu. Appl. Biol. 29:16-17. DOI