Browse > Article
http://dx.doi.org/10.9718/JBER.2022.43.1.61

Cell-laden Gelatin Fiber Contained Calcium Phosphate Biomaterials as a Stem Cell Delivery Vehicle for Bone Repair  

Kim, Seon-Hwa (Interdisciplinary Program of Biomedical Mechanical & Electrical Engineering, Pukyong National University)
Hwang, Changmo (Department of Convergence Medicine, University of Ulsan College of Medicine & Asan Institute for Life Sciences, Asan Medical Center)
Park, Sang-Hyug (Interdisciplinary Program of Biomedical Mechanical & Electrical Engineering, Pukyong National University)
Publication Information
Journal of Biomedical Engineering Research / v.43, no.1, 2022 , pp. 61-70 More about this Journal
Abstract
Natural and synthetic forms of calcium phosphate cement (CPC) have been widely used in bone repair and augmentation. The major challenge of injectable CPC is to deliver the cells without cell death in order to regenerate new bone. The study objective was to investigate for the potential of stem cell-laden gelatin fibers containing injectable, nanocrystalline CPC to function as a delivery system. Gelatin noddle fiber method was developed to delivered cells into nCPC. Experimental groups were prepared by mixing cells with nCPC, mixing cell-laden gelatin fibers with nCPC and mixing cell-laden gelatin fibers containing BMP-2 with nCPC. Media diffusion test was conducted after dissolving the gelatin fibers. SEM examined the generated channels and delivered cell morphology. Fibers mixed with nCPC showed physical setting and hardening within 20 min after injection and showed good shape maintenances. The gelatin fibers mixed nCPC group had several vacant channels generated from the dissolved gelatin. Particularly, proliferation and attachment of the cells were observed inside of the channels. While live cells were not observed in the cell mixed nCPC group, cells delivered with the gelatin fibers into the nCPC showed good viability and increased DNA content with culture. Cell-laden gelatin fiber was a novel method for cell delivery into nCPC without cell damages. Results also indicated the osteogenic differentiation of gelatin fiber delivered cells. We suggest that the cell-laden gelatin fibers mixed with nCPC can be used as an injectable cell delivery vehicle and the addition of BMP-2 to enhances osteogenesis.
Keywords
Nanocrystalline calcium phosphate cement; Gelatin fiber; Cell delivery; Human adipose derived stem cell; Osteogenesis;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Li TL, Tao ZS, Wu XJ, Yang M, Xu HG. Selenium-modified calcium phosphate cement. can accelerate bone regeneration of osteoporotic bone defect. J Bone Miner Metab. 2021;39:934-943.   DOI
2 Huang B, Wu Z, Ding S, Yuan Y, Liu C. Localization and promotion of recombinant. human bone morphogenetic protein2 bioactivity on extracellular matrix mimetic chondroitin sulfate-functionalized calcium phosphate cement scaffolds. Acta Biomater. 2018;71:184-199.   DOI
3 Yuan H, Yang Z, Li Y, Zhang X, De Bruijn JD, De Groot K. Osteoinduction by calcium. phosphate biomaterials. J Mater Sci Mater Med. 1998;9:723-726.   DOI
4 Verret DJ, Ducic Y, Oxford L, Smith J. Hydroxyapatite cement in craniofacial reconstruction. Otolaryngol Head Neck Surg. 2005;133:897-899.   DOI
5 Clarke B. Normal bone anatomy and physiology. Clin J Am Soc Nephrol. 2008;3 Suppl 3:S131-139.   DOI
6 LeGeros RZ. Calcium phosphate-based osteoinductive materials. Chem Rev. 2008;108:4742-4753.   DOI
7 Jeong J, Kim JH, Shim JH, Hwang NS, Heo CY. Bioactive calcium phosphate materials. and applications in bone regeneration. Biomater Res. 2019;23:4.   DOI
8 Li X, Song T, Chen X, et al. Osteoinductivity of Porous Biphasic Calcium Phosphate. Ceramic Spheres with Nanocrystalline and Their Efficacy in Guiding Bone Regeneration. ACS Appl Mater Interfaces. 2019;11:3722-3736.   DOI
9 Vaccaro AR. The role of the osteoconductive scaffold in synthetic bone graft. Orthopedics. 2002;25:s571-578.
10 Cao Q, He Z, Sun WQ, et al. Improvement of calcium phosphate scaffold osteogenesis. in vitro via combination of glutamate-modified BMP-2 peptides. Mater Sci Eng C Mater Biol Appl. 2019;96:412-418.   DOI
11 Winkler T, Sass FA, Duda GN, Schmidt-Bleek K. A review of biomaterials in bone defect. healing, remaining shortcomings and future opportunities for bone tissue engineering: The unsolved challenge. Bone Joint Res. 2018;7:232-243.   DOI
12 Grabowski P. Physiology of Bone. Endocr Dev. 2015;28:33-55.   DOI
13 Baldwin P, Li DJ, Auston DA, Mir HS, Yoon RS, Koval KJ. Autograft, Allograft, and Bone. Graft Substitutes: Clinical Evidence and Indications for Use in the Setting of Orthopaedic Trauma Surgery. J Orthop Trauma. 2019;33:203-213.   DOI
14 Lobb DC, DeGeorge BR, Chhabra AB. Bone Graft Substitutes: Current Concepts and. Future Expectations. J Hand Surg Am. 2019;44:497-505.e492.   DOI
15 Iijima K, Otsuka H. Cell Scaffolds for Bone Tissue Engineering. Bioengineering (Basel). 2020;7.
16 Lin Y, Huang S, Zou R, et al. Calcium phosphate cement scaffold with stem cell co-culture and prevascularization for dental and craniofacial bone tissue engineering. Dent Mater. 2019;35:1031-1041.   DOI
17 Jang JH, Shin S, Kim HJ, et al. Improvement of physical properties of calcium phosphate. cement by elastin-like polypeptide supplementation. Sci Rep. 2018;8:5216.   DOI
18 Kolk A, Handschel J, Drescher W, et al. Current trends and future perspectives of bone. substitute materials - from space holders to innovative biomaterials. J Craniomaxillofac Surg. 2012;40:706-718.   DOI
19 Diaz MA, Branch EA, Paredes LA, Oakley E, Baker CE. Calcium Phosphate Bone Void. Filler Increases Threaded Suture Anchor Pullout Strength: A Biomechanical Study. Arthroscopy. 2020;36:1000-1008.   DOI
20 Ghaffari S, Solati-Hashjin M, Zabihi-Neyshabouri E, Rabiee SM. Novel calcium. phosphate coated calcium silicate-based cement: in vitro evaluation. Biomed Mater. 2020;15:035008.   DOI
21 Ahlfeld T, Doberenz F, Kilian D, et al. Bioprinting of mineralized constructs utilizing. multichannel plotting of a self-setting calcium phosphate cement and a cell-laden bioink. Biofabrication. 2018;10:045002.   DOI
22 Wong SK, Wong YH, Chin KY, Ima-Nirwana S. A Review on the Enhancement of Calcium. Phosphate Cement with Biological Materials in Bone Defect Healing. Polymers (Basel). 2021;13.
23 Liu M, Lv Y. Reconstructing Bone with Natural Bone Graft: A Review of In Vivo Studies. in Bone Defect Animal Model. Nanomaterials (Basel). 2018;8.
24 Xu HH, Simon CG. Self-hardening calcium phosphate composite scaffold for bone. tissue engineering. J Orthop Res. 2004;22:535-543.   DOI
25 Levingstone TJ, Herbaj S, Dunne NJ. Calcium Phosphate Nanoparticles for Therapeutic. Applications in Bone Regeneration. Nanomaterials (Basel). 2019;9.