Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Cell-laden Gelatin Fiber Contained Calcium Phosphate Biomaterials as a Stem Cell Delivery Vehicle for Bone Repair

세포 함유 젤라틴 파이버 응용을 통한 골 재생 유도용 인산칼슘 생체재료 세포 탑재 연구

  • Kim, Seon-Hwa (Interdisciplinary Program of Biomedical Mechanical & Electrical Engineering, Pukyong National University) ;
  • Hwang, Changmo (Department of Convergence Medicine, University of Ulsan College of Medicine & Asan Institute for Life Sciences, Asan Medical Center) ;
  • Park, Sang-Hyug (Interdisciplinary Program of Biomedical Mechanical & Electrical Engineering, Pukyong National University)
  • 김선화 (부경대학교 4차산업융합바이오닉스공학과) ;
  • 황창모 (울산대학교 의과대학 융합의학과) ;
  • 박상혁 (부경대학교 4차산업융합바이오닉스공학과)
  • Received : 2022.02.10
  • Accepted : 2022.02.18
  • Published : 2022.02.28
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Abstract

Natural and synthetic forms of calcium phosphate cement (CPC) have been widely used in bone repair and augmentation. The major challenge of injectable CPC is to deliver the cells without cell death in order to regenerate new bone. The study objective was to investigate for the potential of stem cell-laden gelatin fibers containing injectable, nanocrystalline CPC to function as a delivery system. Gelatin noddle fiber method was developed to delivered cells into nCPC. Experimental groups were prepared by mixing cells with nCPC, mixing cell-laden gelatin fibers with nCPC and mixing cell-laden gelatin fibers containing BMP-2 with nCPC. Media diffusion test was conducted after dissolving the gelatin fibers. SEM examined the generated channels and delivered cell morphology. Fibers mixed with nCPC showed physical setting and hardening within 20 min after injection and showed good shape maintenances. The gelatin fibers mixed nCPC group had several vacant channels generated from the dissolved gelatin. Particularly, proliferation and attachment of the cells were observed inside of the channels. While live cells were not observed in the cell mixed nCPC group, cells delivered with the gelatin fibers into the nCPC showed good viability and increased DNA content with culture. Cell-laden gelatin fiber was a novel method for cell delivery into nCPC without cell damages. Results also indicated the osteogenic differentiation of gelatin fiber delivered cells. We suggest that the cell-laden gelatin fibers mixed with nCPC can be used as an injectable cell delivery vehicle and the addition of BMP-2 to enhances osteogenesis.

Keywords

Acknowledgement

본 연구는 부경대학교 자율창의학술연구비(2019년CD20201462)에 의하여 수행되었음.

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