• Journal of Mushroom
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• v.17 no.3
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• pp.126-131
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• 2019

This study sought to identify the optimum substrate composition for the stable bottle cultivation of Sparassis latifolia. The main substrate was fermented larch sawdust. Six nutrient sources were mixed at a maximum volume ratio of 20%. The fresh weight of fruit body was the highest at 128.5 g for GMSL69033 and 126.6 g for 'Neoul' in the treatments of beet pulp and corn flour in a volume ratio of 15:5. In addition, the total cultivation period was 94 days, which was shorter than that required for other treatments. The selected substrate characteristics were pH 4.7, C:N (carbon to nitrogen) ratio of 106:4, moisture content of 70%, and air filling porosity of 38%. We plan to develop new income items through research on mycelial incubation and fruit body growth conditions.

• The Korean Journal of Mycology
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• v.51 no.2
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• pp.69-80
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• 2023

The cultivation conditions of shiitake mushrooms (Lentinula edodes) influence the production and quality of fruiting bodies. We conducted this study to improve the productivity and quality of shiitake mushrooms by modifying the cultivation conditions. Two types of spawns (sawdust and liquid spawn) were used, and corn flour was used as a nutritional source for the sawdust medium. A blue light-emitting diode (LED; 300 lux) was also used instead of a white LED during the incubation period. Sanbaekhyang was used as the experimental variety. When using corn flour, the mycelial growth rate increased 1.1 to 2.7 times the growth rate of the control up to 21 days of incubation, and the weight loss rate of the media was also higher. Mushroom productivity increased 1.2 times when the liquid spawn was used compared to when the sawdust spawn was used, and the blue LED also increased fruiting body production by 1.1 times compared to the white LED. Mushroom productivity increased when the liquid spawn was used, and the blue LED also increased fruiting body production. Fruiting body weight and the size of the cap were greater when sawdust spawn was used. The fruiting body weight and the stipe diameter were greater when the blue LED was used. Taste analysis showed that the saltiness increased when corn flour was used, and the sourness increased when the blue LED was used.

• Journal of Mushroom
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• v.10 no.2
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• pp.57-62
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• 2012

The plastic culture bottle cap types and accumulated concentration of carbon dioxide, media humidity in the process of medium culture, chitin content and yield were observed in Pleurotus ostreatus 850ml bottle in Iksan, Jeollabuk-do, Korea, during 2011. The concentration of carbon dioxide in the process of medium culture was the highest after 8~9 days cultivation irrespective of cap sizes and types. The accumulated concentration of carbon dioxide in size cap of 29~41mm was 6.5~4.0% in the upper-under perforation hole of cap and 9.0~6.5% in the under perforation hole of cap. The upper-under 23~33mm perforation hole and under 29mm perforation hole of caps in the 850ml bottle were best condition for cultivation of mushroom and increased fruit body, 15.8~21.2% and 20%, respectively. However, the upper-under & under 41mm perforation hole of fruit body were decreased 60.7% and 23.6%, respectively. Also it was weak, lose vitality and the lower of biologically activity substance because the upper medium humidity was too dry.

• Journal of Mushroom
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• v.10 no.2
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• pp.68-73
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• 2012

The plastic culture bottle cap types and accumulated concentration of carbon dioxide, media humidity in the process of medium culture and yield were observed in Pleurotus ostreatus 1,100ml bottle in Iksan, Jeollabuk-do, Korea, during 2011. The concentration of carbon dioxide in the process of medium culture was the highest after 6~11days cultivation irrespective of cap sizes and types. The upper-under 19~38mm perforation hole and under 26~47mm perforation hole of caps in the 1,100ml bottle were best condition for cultivation of mushroom and increased fruit body, 11.4~23.8% and 6.5~17.9%, respectively. However, the upper-under 33mm perforation hole of fruit body were decreased 23.8%. Also it was weak, lose vitality and the lower of biologically activity substance because the upper medium humidity was too dry.

• Mycobiology
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• v.38 no.1
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• pp.7-12
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• 2010

A green mold species that has not previously been reported in Korea was isolated from oak log beds used for shiitake (Lentinula edodes) cultivation that were infested by mushroom flies. In this study, we identify the mold species as Gliocladium viride (an anamorph of Hypocrea lutea) and describe its mycological properties. The fungus was cottony on both potato dextrose agar (PDA) and Czapek yeast extract agar (CYA), but was colored white on PDA and became yellowish green and brown on CYA. Mycelial growth on PDA attained a diameter of 73 mm at $30^{\circ}C$ after 5 days. The fungus grew faster on malt extract agar (> 80 mm, 5 days at $25^{\circ}C$) compared to CYA and PDA (< 68 mm, 5 days at $25^{\circ}C$). Penicillate conidiophores of the fungus are hyaline, smooth walled, branching above typically in four stages, and $120\sim240\;{\mu}m$ in length. Club-shaped or slender phialides are formed on the metulae. Conidia of the fungus were ovate and elliptic, yellowish brown and green, and $2.5\sim3.0\;{\mu}m\times1.8\sim2.3\;{\mu}m$ in size. Typically, slimy conidia are formed in a mass and colored brown to dark green to almost black. The internal transcribed spacer rDNA and translation elongation factor 1 alpha gene sequences of the fungus isolated here show 99% identity with previously identified G. viride strains.

• Journal of Mushroom
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• v.2 no.4
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• pp.225-227
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• 2004

Hukbaek oyster mushroom were bred and cultivated one after mating spores from Aenutari 1-ho irradiated to X-ray and Byungnutari 1-ho. The major charateristics of the mushroom are showing that pileus is black-brown and stems are pure-white and straight. The optimum temperature for the mycelial growth was around $25{\sim}30^{\circ}C$ and that for the pinheading and growth of fruitbody was around $14{\sim}16^{\circ}C$. Around 20 days at $20^{\circ}C$ were required for incubation time and the yield was shown high by 142.8g/bottle. When the cultivating temperature is over $17^{\circ}C$ the color of pileus becomes gray-brown.

• Journal of Mushroom
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• v.2 no.4
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• pp.218-221
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• 2004

Jinmi oyster mushroom(Pleurotus ostreatus) were bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2003. This oyster mushroom were bred and cultivated one after mating single spores collected from K3-2 and ASI2018-249. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and infundibuliform pileus. The optimum temperature for the mycelial growth was around $25{\sim}30^{\circ}C$ and that for the pinheading and growth of fruitbody was around $15{\pm}1^{\circ}C$. Around 22 days at $20^{\circ}C$ were required for incubation of Jinmi and the yield was shown high by 145.9g/bottle.

• The Korean Journal of Mycology
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• v.18 no.2
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• pp.89-96
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• 1990

This study was conducted to find out the effects of Thiabendazole on controlling green mold causing serious damage to oyster mushroom, Pleurotus spp. during the cultivation. In vitro, the strains of oyster mushroom such as ASI 2018, 2072 and 2016 were inhibited by 500 ppm of the fungicide, but the strain of ASI 2001 and ASI 2070 was inhibited by 100 and 500 ppm on oatmeal agar, respectively. The mycelial growth of the oyster mushroom started to be inhibition by soak treatment at a 0.2g/1000 ml aqueous solution of the fungicide. When the oyster mushroom and green mold inoculated both or separately on the substrates of soak treatment, the green mold did not grow at all, but the oyster mushroom grown well. The maximum control effect of the green mold showed when $2g/m^2\;and\;5g/m^2$ of the fungicide was sprayed on the surface of substrates before pasteurization. The highest yield of the sporophores of oyster mushroom was obtained from $5g/m^2$ treatment.

• The Korean Journal of Mycology
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• v.30 no.2
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• pp.131-135
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• 2002

The optimum conditions for the production of Lentinus edodes mycelia and it's hypoglycemic effect was studied. Optimum pH and temperature for the production of mycelia in shaken flask culture were 5 and $30^{\circ}C$, respectively, for 24 days cultivation. Culture period for maximum production of mycelia (8.13 g/l) in 5-l jar fermenter cultivation was shortened as much as 6 days compared to shaken flask culture. The mycelial dose of 5 % proved almost equally effective in lowering the plasma glucose, total cholesterol, and triglyceride level as much as 23.0, 20.7, and 27.1%, respectively. The values of alanine transaminase and aspartate transaminase were also estimated and exhibited a substantial reduction (29.6 and 34.3%, respectively) in activity after the administration of dried mycelia.

• Journal of Mushroom
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• v.4 no.1
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• pp.39-42
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• 2006

Cauliflower mushroom(Sparassis crispa) contains much amount of ${\beta}$-glucan, which make lots of farmer want to cultivate the mushroom. Since a practical cultivation method is not provided yet, the mushroom is considered as a difficult crop to deal with. In this study, we tried to develope a simple method to cultivate the mushroom by use of coniferous sawdust-based medium with wheat flour and molasses. There was no significant differences between the sawdust spawn and the liquid spawn for the mycelial growth of the mushroom. The cold shock in $4^{\circ}C$ for a day was thought to be the best way to seduce primordium formation. The sawdust medium of Pseudotsuga menziesii mixed with wheat flour, corn chip, cottonseed meal and 10% molasses was showed the best yield with 41% followed by that of Larix leptolepis mixed with the same additives with 37% of yield. There was good relations between the fruit-body production and the weight loss of the sawdust substrate.