• 대한기계학회논문집A
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• 제33권6호
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• pp.568-572
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• 2009

We present a ternary microfluidic multiplexer unit, capable to address three flow channels using a pair of control lines with two different threshold pressure valves. The previous binary multiplexer unit addresses only two flow channels using a pair of control line with identical threshold pressure valves, thus addressing $2^{n/2}$ flow channels using n control lines. The present ternary multiplexer addressing three flow channels using a pair of control lines, however, is capable to address $3^{n/2}$ flow channels using n control lines with two different threshold pressure valves. In the experimental study, we characterized the threshold pressure and the response time of the valves used in the ternary multiplexer. From the experimental observation, we also verified that the present ternary multiplexer unit could be operated by two equivalent valve operating conditions: the different static pressures and dynamic pressures at different duty ratio. And then, $3{\times}3$ well array stacking ternary multiplexers in serial is addressed in cross and plus patterns, thus demonstrating the individual flow channel addressing capability of the ternary multiplexer. Thus, the present ternary multiplexer reduces the number of control lines for addressing flow channels, achieving the high well control efficiency required for simple and compact microfluidic systems.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2022년도 봄 학술논문 발표대회
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• pp.251-252
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• 2022

The microfluidic controlled droplet manufacturing system is one of the most powerful methods for capsule manufacturing. The microfluidic control method can control the type and size of the capsule by changing the size and configuration of the channel. In addition, by increasing the number of channels, capsules of uniform size can be mass-produced. In this paper, a capsule manufacturing system including flow-focusing and T junction method was designed. In addition, the effectiveness of this system was verified by manufacturing multi-emulsion capsules with a size of 2.2 to 3 mm.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.2349-2351
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• 2003

This paper reports a novel microfluidic system, by which microfluidic delivery, transport and control can be digitally realized in femtoliter scale. Microelectronic grade $N_2$ from a pressurized canister was passed through HPLC tubing into a micro injector. The micro injector was driven and controlled digitally by the control system that can apply various control parameters such as pulse frequencies. A front-end of micro nozzle was inserted the dyed oil to collect droplets injected. The diameter of a droplet was measured by a microscope and a CCD camera, and then its volume can be calculated on the assumption that the droplet is spherical. The micro nozzles were simply pulled in glass capillary tubes by the micro puller self-made, and the geometry parameters of the micro nozzles can be adjusted easily. Experiments have successfully been carried out, and the results demonstrated that the proposed digital micro injector possesses three significant advantages : precise ultra-small liquid volume in femtoliter scale, digital microfluidic control and micro devices fabricated by simple glass process, not based on IC process.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.146-153
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• 2006

A passive microfluidic delivery system using hydrophobic valving and pneumatic control was devised for microfluidic handling on a chip. The microfluidic metering, cutting, transport, and merging of two liquids on the chip were correctly performed. The error range of the accuracy of microfluid metering was below 4% on a 20 nL scale, which showed that microfluid was easily manipulated with the desired volume on a chip. For a study of the feasibility of biochemical reactions on the chip, a single enzymatic reaction, such as ${\beta}-galactosidase$ reaction, was performed. The detection limit of the substrate, i.e. fluorescein $di-{\beta}-galactopyranoside$ (FDG) of the ${\beta}-galactosidase$ (6.7 fM), was about 76 pM. Additionally, multiple biochemical reactions such as in vitro protein synthesis of enhanced green fluorescence protein (EGFP) were successfully demonstrated at the nanoliter scale, which suggests that our microfluidic chip can be applied not only to miniaturization of various biochemical reactions, but also to development of the microfluidic biochemical reaction system requiring a precise nano-scale control.

• 한국생산제조학회지
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• 제22권2호
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• pp.235-240
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• 2013

This paper presents a disposable microfluidic infusion pump using the restoring force of elastomeric membrane of Polydimethylsiloxane. Microfluidic infusion pump consists of hydraulic resistance control part, elastomeric blister actuator part, drug inlet and outlet. Expanded elastomeric blister actuator continuously pushes liquid in the chamber to outlet. At same time, microchannel diameter near the outlet was controlled by thin elastomeric membrane in hydraulic resistance control part. Eventually flow rate of infused liquid is controlled by air pressure. In experimental study, the amount of the filled liquid in the blister is precisely controlled by the height of the blister. Flow rate of infused liquid could be controlled, that is, controlled release of the drug over time was possible by adjusting hydraulic resistance and restoring pressure with the blister actuator.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회B
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• pp.2688-2691
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• 2008

This study addresses a microfluidic method to uniformly form diacetylene (DA) liposomes and control their size. DA liposomes are biochemical sensor materials with a unique property such that when they are polymerized to polydiacetylene (PDA) they exhibit non-fluorescent blue to fluorescent red phase transition upon chemical or thermal stress. The liposome size and distribution are important because they significantly affect the phase transition. So far, DA Liposomes, have been prepared by mixing of bulk phases leading to heterogeneous, polydisperse distribution in size. Therefore, additional post-processes are required such as sonication or membrane extrusion to obtain an appropriate size of liposomes. Here, we report a novel strategy using a microfluidic chip and hydrodynamic focusing to form DA liposomes and control their size. Preliminary results obtained by scanning electron microscope (SEM) and dynamic light scattering (DLS) show that the microfluidic strategy generates more monodispersed liposomes than a bulk method.

• 한국정보통신학회논문지
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• 제17권2호
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• pp.447-452
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• 2013

미세유체소자(microfluidic device)는 미생물과 관련된 다양한 작업들에 대해서 정확한 제어를 제공할 수 있다. 본 논문에서는 미세유체 소자와 디지털 홀로그래피 마이크로스코피 기술로 구성된 시스템을 구성하고 미생물의 3D 이미징과 세그먼테이션을 설명한다. 각각의 미생물은 미세유체 채널을 통하여 흘러가며 홀로그래피 마이크로스코피가 홀로그램을 기록한다. 기록된 홀로그램은 Fresnel 변환을 통하여 컴퓨터적으로 복원되며, 복원된 영상의 위상성분을 이용하여 미생물의 위치 정보를 찾기 위한 세그먼테이션을 수행한다. 제안하는 방법의 유용함을 설명하기 위하여 광학 실험을 수행하고 그 결과를 나타내었다.

• Korean Chemical Engineering Research
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• 제52권2호
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• pp.141-153
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• 2014

액적기반의 미세유체 시스템은 마이크로 시험관으로서 화학, 생물학 연구에 적용하기 위해 개발되었다. 미세유체 시스템에서 피코부피(picoliter)의 매우 작은 액적은 소형화된 시스템 내에서 잘 정형화 되고 구획화된 반응기로 제공되어 진다. 매우 작은 액적에서의 반응은 자동화된 초고속 대용량 스크리닝 시스템을 통하여 저가이면서 고효율적으로 수행될 수 있다. 본 총설에서는 액적 기반의 미세유체시스템의 기능들인 액적 형성, 정교한 액적 제어, 다양한 응용분야에 대해 소개하고자 한다. 또한 화학적, 생물학적 새로운 응용분야에 관해 알아보고, 기존의 방법과 비교하여 액적기반의 미세유체 시스템이 갖는 장점에 관해 논의하고자 한다.

• 대한기계학회논문집B
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• 제34권3호
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• pp.251-258
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• 2010

본 논문은 다기능 미소유체시스템의 일체형 패키징을 위한 MSI (microfluidic system interface) 기술을 제안하고, 이를 설계, 제작, 시험 평가하였다. MSI 기술을 통해 플러그 방식의 유체 인터커넥터, 유체제어를 위한 미소밸브, 광학 인터페이스를 위한 광학창을 유체시스템에 일체형으로 쉽게 구현할 수 있었다. MSI 기술의 유용성을 보이기 위해 미소 유전자시료전처리시스템에 적용되었으며, 미소 유전자시료전처리시스템은 세포정제, 세포분리, 세포용해, DNA 고체상추출, 중합효소연쇄반응, 그리고 모세관전기영동 기능으로 구성되었다. 나아가 MSI 기술이 적용된 미소 유전자시료전처리시스템의 DNA 고체상추출 및 중합효소연쇄반응의 실험결과로부터 MSI가 미소유체시스템을 위한 실용적 패키징 기술임이 검증되었다.

• Korean Chemical Engineering Research
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• 제48권5호
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• pp.545-555
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• 2010

최근 액적 기반의 미세유체 시스템은 물리, 화학, 생물학등의 기초과학과 재료과학 분야까지 매우 폭넓게 활용되고 각광받고 있는 기술분야이다. 본 총설은 액적기반 미세유체 시스템의 미세유체 반응기 제작 기술, 액적 형성 원리, 액적 혼합 및 제어, 그리고 새로운 기능성 재료의 합성등의 폭넓은 응용분야에 관해 자세하게 소개하고자 한다. 더불어 액적기반 미세유체 시스템의 가장 큰 장점인 입자의 크기 조절 방법, 형태, 모양 및 구조의 제어 기술에 관해 논의하고자 한다.