Droplet-based Microfluidic Device for High-throughput Screening |
Jeong, Heon-Ho
(Department of Chemical Engineering, Chungnam National University)
Noh, Young-Moo (Department of Chemical Engineering, Chungnam National University) Jang, Sung-Chan (Department of Chemical Engineering, Chungnam National University) Lee, Chang-Soo (Department of Chemical Engineering, Chungnam National University) |
1 | Whitesides, G. M., "The Origins and the Future of Microfluidics," Nature, 442(7101), 368-373(2006). DOI ScienceOn |
2 | Theberge, A. B., Courtois, F., Schaerli, Y., Fischlechner, M., Abell, C., Hollfelder, F. and Huck, W. T. S., "Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology," Angew. Chem. Int. Edit., 49(34), 5846-5868(2010). DOI ScienceOn |
3 | Huebner, A., S. Sharma, M. Srisa-Art, F. Hollfelder, J. B. Edel, and A. J. Demello, "Microdroplets: A Sea of Applications?," Lab Chip, 8(8), 1244-1254(2008). DOI ScienceOn |
4 | Song, H. M. and Lee, C. S., "Simple Fabrication of Functionalized Surface with Polyethylene Glycol Microstructure and Glycidyl Methacrylate Moiety for the Selective Immobilization of Proteins and Cells," Korean J. Chem. Eng., 25(6), 1467-1472(2008). 과학기술학회마을 DOI |
5 | Lee, J. H., Kim, H. E., Im, J. H., Bae, Y. M., Choi, J. S., Huh, K. M. and Lee, C. S., "Preparation of Orthogonally Functionalized Surface Using Micromolding in Capillaries Technique for the Control of Cellular Adhesion," Colloids Surf. B: Biointerfaces, 64(1), 126-134(2008). DOI ScienceOn |
6 | Chiu, D. T., Lorenz, R. M. and Jeffries, G. D. M., "Droplets for Ultrasmall-Volume Analysis," Anal. Chem., 81(13), 5111-5118(2009). DOI ScienceOn |
7 | Kim, K. P., Kim, Y. G., Choi, C. H., Kim, H. E., Lee, S. H., Chang, W. S. and Lee, C. S., "In situ Monitoring of Antibiotic Susceptibility of Bacterial Biofilms in a Microfluidic Device," Lab Chip, 10(23), 3296-9(2010). DOI ScienceOn |
8 | Choi, C. H., Prasad, N., Lee, N. R. and Lee, C. S., "Investigation of Microchannel Wettability on the Formation of Droplets and Efficient Mixing in Microfluidic Devices," Biochip J., 2(1), 27-32 (2008). |
9 | Teh, S. Y., Lin, R., Hung, L. H. and Lee, A. P., "Droplet Microfluidics," Lab Chip, 8(2), 198-220(2008). DOI ScienceOn |
10 | Jeong, H. H., Lee, S. H., Kim, J. M., Kim, H. E., Kim, Y. G., Yoo, J. Y., Chang, W. S. and Lee, C. S., "Microfluidic Monitoring of Pseudomonas Aeruginosa Chemotaxis Under the Continuous Chemical Gradient," Biosens. Bioelectron., 26(2), 351-6(2010). DOI ScienceOn |
11 | Kaigala, G. V., Hoang, V. N., Stickel, A., Lauzon, J., Manage, D., Pilarski, L. M. and Backhouse, C. J., "An Inexpensive and Portable Microchip-Based Platform for Integrated RT-PCR and Capillary Electrophoresis," Analyst, 133(3), 331-338(2008). DOI ScienceOn |
12 | Jeong, H. H., Lee, S. H. and Lee, C. S., "Pump-less Static Microfluidic Device for Analysis of Chemotaxis of Pseudomonas Aeruginosa Using Wetting and Capillary Action," Biosens. Bioelectron., 47, 278-84(2013). DOI ScienceOn |
13 | Song, H. and Ismagilov, R. F., "Millisecond Kinetics on a Microfluidic Chip Using Nanoliters of Reagents," J. Am. Chem. Soc., 125(47), 14613-14619(2003). DOI ScienceOn |
14 | Jung, J. H., Choi, C. H., Hwang, T. S. and Lee, C. S., "Efficient In situ Production of Monodisperse Polyurethane Microbeads in Microfluidic Device using Increase of Residence Time of Droplets," Biochip J., 3(1), 44-49(2009). |
15 | Jung, S. Y., Liu, Y. and Collier, C. P., "Fast Mixing and Reaction Initiation Control of Single-enzyme Kinetics in Confined Volumes," Langmuir, 24(9), 4439-4442(2008). DOI ScienceOn |
16 | Garstecki, P., Fuerstman, M. J., Stone, H. A. and Whitesides, G. M., "Formation of Droplets and Bubbles in a Microfluidic Tjunction - scaling and Mechanism of Break-up," Lab Chip, 6(3), 437-446(2006). DOI ScienceOn |
17 | Clausell-Tormos, J., Lieber, D., Baret, J. C., El-Harrak, A., Miller, O. J., Frenz, L., Blouwolff, J., Humphry, K. J., Koster, S., Duan, H., Holtze, C., Weitz, D. A., Griffiths, A. D. and Merten, C. A., "Droplet-based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms (vol 15, pg 427, 2008)," Chem. Biol., 15(8), 875-875 (2008). DOI ScienceOn |
18 | Liau, A., Karnik, R., Majumdar, A. and Cate, J. H. D., "Mixing Crowded Biological Solutions in Milliseconds," Anal. Chem., 77(23), 7618-7625(2005). DOI ScienceOn |
19 | Tan, Y. C., Ho, Y. L. and Lee, A. P., "Droplet Coalescence by Geometrically Mediated Flow in Microfluidic Channels," Microfluid. Nanofluid., 3(4), 495-499(2007). DOI ScienceOn |
20 | Huebner, A., Bratton, D., Whyte, G., Yang, M., deMello, A. J., Abell, C. and Hollfelder, F., "Static Microdroplet Arrays: a Microfluidic Device for Droplet Trapping, Incubation and Release for Enzymatic and Cell-based Assays," Lab Chip, 9(5), 692-698(2009). DOI ScienceOn |
21 | Yun, J. X., Tu, C. M., Lin, D. Q., Xu, L. H., Guo, Y. T., Shen, S. C., Zhang, S. H., Yao, K. J., Guan, Y. X. and Yao, S. J., "Microchannel Liquid-flow Focusing and Cryo-polymerization Preparation of Supermacroporous Cryogel Beads for Bioseparation," J. Chromatogr. A, 1247, 81-88(2012). DOI ScienceOn |
22 | Simon, M. G., Lin, R., Fisher, J. S. and Lee, A. P., "A Laplace Pressure Based Microfluidic Trap for Passive Droplet Trapping and Controlled Release," Biomicrofluidics, 6(1), (2012). |
23 | Shim, J. U., Cristobal, G., Link, D. R., Thorsen, T., Jia, Y. W., Piattelli, K. and Fraden, S., "Control and Measurement of the Phase Behavior of Aqueous Solutions Using Microfluidics," J. Am. Chem. Soc., 129(28), 8825-8835(2007). DOI ScienceOn |
24 | Schafle, C., Bechinger, C., Rinn, B., David, C. and Leiderer, P., "Cooperative Evaporation in Ordered Arrays of Volatile Droplets,"Phys. Rev. Lett., 83(25), 5302-5305(1999). DOI ScienceOn |
25 | Lee, W. S., Jambovane, S., Kim, D. and Hong, J. W., "Predictive Model on Micro Droplet Generation Through Mechanical Cutting," Microfluid. Nanofluid., 7(3), 431-438(2009). DOI ScienceOn |
26 | Fair, R. B., "Digital Microfluidics: is a True Lab-on-a-chip Possible?," Microfluid. Nanofluid., 3(3), 245-281(2007). DOI ScienceOn |
27 | Du, W. B., Sun, M., Gu, S. Q., Zhu, Y. and Fang, Q., "Automated Microfluidic Screening Assay Platform Based on Drop Lab," Anal. Chem., 82(23), 9941-9947(2010). DOI ScienceOn |
28 | Ahn, B., Lee, K., Lee, H., Panchapakesan, R. and Oh, K. W., "Parallel Synchronization of Two Trains of Droplets Using a Railroad-like Channel Network," Lab Chip, 11(23), 3956-3962(2011). DOI ScienceOn |
29 | Zeng, S. J., Li, B. W., Su, X. O., Qin, J. H. and Lin, B. C., "Microvalve-actuated Precise Control of Individual Droplets in Microfluidic Devices," Lab Chip, 9(10), 1340-1343(2009). DOI ScienceOn |
30 | Link, D. R., Anna, S. L., Weitz, D. A. and Stone, H. A., "Geometrically Mediated Breakup of Drops in Microfluidic Devices," Phys. Rev. Lett., 92(5), (2004). |
31 | Christopher, G. F., Bergstein, J., End, N. B., Poon, M., Nguyen, C. and Anna, S. L., "Coalescence and Splitting of Confined Droplets at Microfluidic Junctions," Lab Chip, 9(8), 1102-1109(2009). DOI ScienceOn |
32 | Choi, J. H., Lee, S. K., Lim, J. M., Yang, S. M. and Yi, G. R., "Designed Pneumatic Valve Actuators for Controlled Droplet Breakup and Generation," Lab Chip, 10(4), 456-461(2010). DOI ScienceOn |
33 | Churski, K., Korczyk, P. and Garstecki, P., "High-throughput Automated Droplet Microfluidic System for Screening of Reaction Conditions," Lab Chip, 10(7), 816-818(2010). DOI ScienceOn |
34 | Hong, J., Choi, M., deMello, A. J. and Edel, J. B., "Interfacial Tension-Mediated Droplet Fusion in Rectangular Microchannels," Biochip J., 3(3), 203-207(2009). |
35 | Niu, X., Gulati, S., Edel, J. B. and deMello, A. J., "Pillar-induced Droplet Merging in Microfluidic Circuits," Lab Chip, 8(11), 1837-1841(2008). DOI ScienceOn |
36 | Mazutis, L. and Griffiths, A. D., "Selective Droplet Coalescence Using Microfluidic Systems," Lab Chip, 12(10), 1800-1806(2012). DOI ScienceOn |
37 | Leung, K., Zahn, H., Leaver, T., Konwar, K. M., Hanson, N. W., Page, A. P., Lo, C. C., Chain, P. S., Hallam, S. J. and Hansen, C. L., "A Programmable Droplet-based Microfluidic Device Applied to Multiparameter Analysis of Single Microbes and Microbial Communities," Proc. Natl. Acad. Sci. USA, 109(20), 7665-7670(2012). DOI |
38 | Tan, W. H. and Takeuchi, S., "A Trap-and-release Integrated Microfluidic System for Dynamic Microarray Applications," Proc. Natl. Acad. Sci. USA, 104(4), 1146-1151(2007). DOI ScienceOn |
39 | Chabert, M. and Viovy, J. L., "Microfluidic High-throughput Encapsulation and Hydrodynamic Self-sorting of Single Cells," Proc. Natl. Acad. Sci. USA, 105(9), 3191-3196(2008). DOI ScienceOn |
40 | Bai, Y. P., He, X. M., Liu, D. S., Patil, S. N., Bratton, D., Huebner, A., Hollfelder, F., Abell, C. and Huck, W. T. S., "A Double Droplet Trap System for Studying Mass Transport Across a Droplet-droplet Interface," Lab Chip, 10(10), 1281-1285(2010). DOI ScienceOn |
41 | Cohen, I., Li, H., Hougland, J. L., Mrksich, M. and Nagel, S. R., "Using Selective Withdrawal to Coat Microparticles," Science, 292(5515), 265-267(2001). DOI ScienceOn |
42 | Yiantsios, S. G. and Davis, R. H., "On the Buoyancy-Driven Motion of a Drop Towards a Rigid Surface or a Deformable Interface," J. Fluid. Mech., 217, 547-573(1990). DOI |
43 | Cubaud, T. and Mason, T. G., "Folding of Viscous Threads in Diverging Microchannels," Phys. Rev. Lett., 96(11), (2006). |
44 | Agresti, J. J., Antipov, E., Abate, A. R., Ahn, K., Rowat, A. C., Baret, J. C., Marquez, M., Klibanov, A. M., Griffiths, A. D. and Weitz, D. A., "Ultrahigh-throughput Screening in Drop-based Microfluidics for Directed Evolution (vol 170, pg 4004, 2010)," Proc. Natl. Acad. Sci. USA, 107(14), 6550-6550(2010). DOI |
45 | Miyaura, N. and Suzuki, A., "Palladium-Catalyzed Cross-Coupling Reactions of Organoboron Compounds," Chem. Rev., 95(7), 2457-2483(1995). DOI ScienceOn |
46 | Wang, W., Yang, C., Liu, Y. S. and Li, C. M., "On-demand Droplet Release for Droplet-based Microfluidic System," Lab Chip, 10(5), 559-562(2010). DOI ScienceOn |
47 | Hatakeyama, T., Chen, D. L. and Ismagilov, R. F., "Microgramscale Testing of Reaction Conditions in Solution Using Nanoliter Plugs in Microfluidics with Detection by MALDI-MS," J. Am. Chem. Soc., 128(8), 2518-2519(2006). DOI ScienceOn |
48 | Theberge, A. B., Whyte, G., Frenzel, M., Fidalgo, L. M., Wootton, R. C. R. and Huck, W. T. S., "Suzuki-Miyaura Coupling Reactions in Aqueous Microdroplets with Catalytically Active Fluorous Interfaces," Chem Commun., 41, 6225-6227(2009). |
49 | Chan, E. M., Alivisatos, A. P. and Mathies, R. A., "High-temperature Microfluidic Synthesis of CdSe Nanocrystals in Nanoliter Droplets," J. Am. Chem. Soc., 127(40), 13854-13861(2005). DOI ScienceOn |
50 | Jung, J. H., Park, T. J., Lee, S. Y. and Seo, T. S., "Homogeneous Biogenic Paramagnetic Nanoparticle Synthesis Based on a Microfluidic Droplet Generator," Angew. Chem. Int. Edit., 51(23), 5634-5637(2012). DOI ScienceOn |
51 | Abou Hassan, A., Sandre, O., Cabuil, V. and Tabeling, P., "Synthesis of Iron Oxide Nanoparticles in a Microfluidic Device: Preliminary Results in a Coaxial Flow Millichannel," Chem Commun., 15, 1783-1785(2008). |
52 | Zhao, C. X., He, L. Z., Qiao, S. Z. and Middelberg, A. P. J., "Nanoparticle Synthesis in Microreactors," Chem Eng Sci., 66(7), 1463-1479(2011). DOI ScienceOn |
53 | Kim, J., Chung, Y. M., Kang, S. M., Choi, C. H., Kim, B. Y., Kwon, Y. T., Kim, T. J., Oh, S. H. and Lee, C. S., "Palladium Nanocatalysts Immobilized on Functionalized Resin for the Direct Synthesis of Hydrogen Peroxide from Hydrogen and Oxygen," ACS Catal., 2(6), 1042-1048(2012). DOI ScienceOn |
54 | Bharde, A., Rautaray, D., Bansal, V., Ahmad, A., Sarkar, I., Yusuf, S. M., Sanyal, M. and Sastry, M., "Extracellular Biosynthesis of Magnetite Using Fungi," Small, 2(1), 135-141(2006). DOI ScienceOn |
55 | Lu, A. H., Salabas, E. L. and Schuth, F., "Magnetic Nanoparticles: Synthesis, Protection, Functionalization, and Application," Angew. Chem. Int. Edit., 46(8), 1222-1244(2007). DOI ScienceOn |
56 | Jeong, H. H., Kim, Y. G., Jang, S. C., Yi, H. M. and Lee, C. S., "Profiling Surface Glycans on Live Cells and Tissues Using Quantum Dot-lectin Nanoconjugates," Lab Chip, 12(18), 3290-3295(2012). DOI |
57 | Duraiswamy, S. and Khan, S. A., "Plasmonic Nanoshell Synthesis in Microfluidic Composite Foams," Nano Lett, 10(9), 3757-3763(2010). DOI ScienceOn |
58 | Rubin, A. E., Tummala, S., Both, D. A., Wang, C. C. and Delaney, E. J., "Emerging Technologies Supporting Chemical Process R&D and Their Increasing Impact on Productivity in the Pharmaceutical Industry," Chem. Rev., 106(7), 2794-2810(2006). DOI ScienceOn |
59 | An, H. Y. and Cook, P. D., "Methodologies for Generating Solution-phase Combinatorial Libraries," Chem. Rev., 100(9), 3311-3340(2000). DOI ScienceOn |
60 | Theberge, A. B., Mayot, E., El Harrak, A., Kleinschmidt, F., Huck, W. T. S. and Griffiths, A. D., "Microfluidic Platform for Combinatorial Synthesis in Picolitre Droplets," Lab Chip, 12(7), 1320-1326(2012). DOI ScienceOn |
61 | Schaerli, Y., Wootton, R. C., Robinson, T., Stein, V., Dunsby, C., Neil, M. A. A., French, P. M. W., deMello, A. J., Abell, C. and Hollfelder, F., "Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets," Anal. Chem., 81(1), 302-306(2009). DOI ScienceOn |
62 | Zhang, C. S., Xing, D. and Li, Y. Y., "Micropumps, Microvalves, and Micromixers Within PCR Microfluidic Chips: Advances and Trends," Biotechnol Adv, 25(5), 483-514(2007). DOI ScienceOn |
63 | Hatch, A. C., Fisher, J. S., Tovar, A. R., Hsieh, A. T., Lin, R., Pentoney, S. L., Yang, D. L. and Lee, A. P., "1-Million Droplet Array with Wide-field Fluorescence Imaging for Digital PCR," Lab Chip., 11(22), 3838-3845(2011). DOI ScienceOn |
64 | Kiss, M. M., Ortoleva-Donnelly, L., Beer, N. R., Warner, J., Bailey, C. G., Colston, B. W., Rothberg, J. M., Link, D. R. and Leamon, J. H., "High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets," Anal. Chem., 80(23), 8975-8981(2008). DOI ScienceOn |
65 | Fallah-Araghi, A., Baret, J. C., Ryckelynck, M. and Griffiths, A. D., "A Completely in vitro Ultrahigh-throughput Droplet-based Microfluidic Screening System for Protein Engineering and Directed Evolution," Lab Chip, 12(5), 882-891(2012). DOI ScienceOn |
66 | Tawfik, D. S. and Griffiths, A. D., "Man-made Cell-like Compartments for Molecular Evolution," Nat. Biotechnol., 16(7), 652-656(1998). DOI ScienceOn |
67 | Edd, J. F., Carlo, D. D, Humphry, K. J., Koster, S., Irimia, D., Weitz, D. A. and Toner, M., "Controlled Encapsulation of Singlecells Into Monodisperse Picolitre Drops," Lab Chip, 8(8), 1262-1264(2008). DOI ScienceOn |
68 | Juul, S., Nielsen, C. J. F., Labouriau, R., Roy, A., Tesauro, C., Jensen, P. W., Harmsen, C., Kristoffersen, E. L., Chiu, Y. L., Frohlich, R., Fiorani, P., Cox-Singh, J., Tordrup, D., Koch, J., Bienvenu, A. L., Desideri, A., Picot, S., Petersen, E., Leong, K. W., Ho, Y. P., Stougaard, M. and Knudsen, B. R., "Droplet Microfluidics Platform for Highly Sensitive and Quantitative Detection of Malaria-Causing Plasmodium Parasites Based on Enzyme Activity Measurement," ACS nano, 6(12), 10676-10683(2012). DOI ScienceOn |
69 | Lee, K. G., Park, T. J., Soo, S. Y., Wang, K. W., Kim, B. H., Park, J. H., Lee, C. S., Kim, D. H. and Lee, S. J., "Synthesis and Utilization of E. coli-Encapsulated PEG-Based Microdroplet Using a Microfluidic Chip for Biological Application," Biotechnol. Bioeng., 107(4), 747-751(2010). DOI ScienceOn |
70 | Joensson, H. N., Samuels, M. L., Brouzes, E. R., Medkova, M., Uhlen, M., Link, D. R. and Andersson-Svahn, H., "Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets," Angew. Chem. Int. Edit., 48(14), 2518-2521(2009). DOI ScienceOn |
71 | Kemna, E. W. M., Schoeman, R. M., Wolbers, F., Vermes, I., Weitz, D. A. and van den Berg, A., "High-yield Cell Ordering and Deterministic Cell-in-droplet Encapsulation Using Dean Flow in a Curved Microchannel," Lab Chip, 12(16), 2881-2887(2012). DOI |
72 | Koster, S., Angile, F. E., Duan, H., Agresti, J. J., Wintner, A., Schmitz, C., Rowat, A. C., Merten, C. A., Pisignano, D., Griffiths, A. D. and Weitz, D. A., "Drop-based Microfluidic Devices for Encapsulation of Single Cells," Lab Chip, 8(7), 1110-1115(2008). DOI ScienceOn |
73 | Boukellal, H., Selimovic, S., Jia, Y. W., Cristobal, G. and Fraden, S., "Simple, Robust Storage of Drops and Fluids in a Microfluidic Device," Lab Chip, 9(2), 331-338(2009). DOI ScienceOn |
74 | El Debs, B., Utharala, R., Balyasnikova, I. V., Griffiths, A. D. and Merten, C. A., "Functional Single-cell Hybridoma Screening Using Droplet-based Microfluidics," Proc. Natl. Acad. Sci. USA, 109(29), 11570-11575(2012). DOI |
75 | Cira, N. J., Ho, J. Y., Dueck, M. E. and Weibel, D. B., "A Selfloading Microfluidic Device for Determining the Minimum Inhibitory Concentration of Antibiotics," Lab Chip, 12(6), 1052-1059(2012). DOI |
76 | Courtois, F., Olguin, L. F., Whyte, G., Theberge, A. B., Huck, W. T. S., Hollfelder, F. and Abell, C., "Controlling the Retention of Small Molecules in Emulsion Microdroplets for Use in Cell-Based Assays," Anal. Chem., 81(8), 3008-3016(2009). DOI ScienceOn |
77 | Li, S. H., Lin, Y. J., Cao, J. G. and Zhang, S. B., "Guanidine/Pd(OAc)(2)-catalyzed Room Temperature Suzuki Cross-coupling Reaction in Aqueous Media Under Aerobic Conditions," J. Org. Chem., 72(11), 4067-4072(2007). DOI ScienceOn |
78 | Prasad, N., Perumal, J., Choi, C. H., Lee, C. S. and Kim, D. P., "Generation of Monodisperse Inorganic-Organic Janus Microspheres in a Microfluidic Device," Adv. Funct. Mater., 19(10), 1656-1662(2009). DOI ScienceOn |
79 | Kovarik, M. L., Gach, P. C., Ornoff, D. M. Wang, Y. L., Balowski, J., Farrag, L. and Allbritton, N. L., "Micro Total Analysis Systems for Cell Biology and Biochemical Assays," Anal. Chem., 84(2), 516-540(2012). DOI ScienceOn |
80 | Hong, J., Choi, M., Edel, J. B. and deMello, A. J., "Passive Selfsynchronized Two-droplet Generation," Lab Chip, 10(20), 2702-2709(2010). DOI ScienceOn |
81 | Hindson, B. J., Ness, K. D., Masquelier, D. A., Belgrader, P., Heredia, N. J., Makarewicz, A. J., Bright, I. J., Lucero, M. Y., Hiddessen, A. L., Legler, T. C., Kitano, T. K., Hodel, M. R., Petersen, J. F., Wyatt, P. W., Steenblock, E. R., Shah, P. H., Bousse, L. J., Troup, C. B., Mellen, J. C., Wittmann, D. K., Erndt, N. G., Cauley, T. H., Koehler, R. T., So, A. P., Dube, S., Rose, K. A., Montesclaros, L., Wang, S. L., Stumbo, D. P., Hodges, S. P., Romine, S., Milanovich, F. P., White, H. E., Regan, J. F., Karlin-Neumann, G. A., Hindson, C. M., Saxonov, S. and Colston, B. W., "High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number," Anal. Chem., 83(22), 8604-8610(2011). DOI ScienceOn |
82 | Brouzes, E., Medkova, M., Savenelli, N., Marran, D., Twardowski, M., Hutchison, J. B., Rothberg, J. M., Link, D. R., Perrimon, N. and Samuels, M. L., "Droplet Microfluidic Technology for Single-cell High-throughput Screening," Proc. Natl. Acad. Sci. USA, 106(34), 14195-14200(2009). DOI ScienceOn |