• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1552-1556
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• 2005

The UCPs are members of the mitochondrial inner membrane transporter family, present in the mitochondrial inner membrane. Their main function is increasing the energy expenditure via diminishing the resulting production of ATP from mitochondrial oxidative phosphorylation instead of yielding dissipative heat. They are associated with the metabolism of fat and regulation of energy expenditure. The UCP gene can be viewed as the candidate gene for chicken fatness. In the present study, RT-PCR and Northern Blot methods were developed to investigate the expression of the UCP gene in ten tissues including heart, liver, spleen, lung, kidney, gizzard, intestine, brain, breast muscle and abdominal fat of chicken. The results of both RT-PCR and Northern Blot methods showed that the UCP gene expressed specific in breast muscle. The expression levels of UCP gene in breast muscles from egg-type and meat-type chickens of hatching, 2, 4, 6 and 8 wk of age were detected by RT-PCR assay and results showed that the expression levels of UCP gene were related to breeds. Expression level of UCP gene in layers was higher than that in broilers at various weeks of age except at 6 wk. The UCP gene's expression was higher at 6 wk and had no significant difference among other weeks of age in broilers; in layers the expression level of UCP gene had no significant difference among weeks of age. The experiment results also showed that insulin could increase the expression level of UCP gene by 40% compared with control group.

• 한국가금학회지
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• 제38권2호
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• pp.137-144
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• 2011

본 시험은 토종닭의 교배 조합이 계육의 지방산과 아미노산 및 핵산 관련 물질 함량에 미치는 영향을 조사하기 위하여 수행되었다. 공시계는 4개 교배 조합으로 발생된 3원 교잡종을 이용하였다. 시험 설계는 A(CS${\times}$B), B(CH${\times}$S), C(RS${\times}$H), D(백세미)로 하였으며, 4처리구에 따라 암수 각각 9반복, 반복당 10수씩($4{\times}2{\times}9{\times}10$) 총 720수를 완전 임의 배치하였다. 시험 사료는 제시한 옥수수-대두박 위주의 준육용계 사료를 자체 제작하여 이용하였다. 5주령 계육의 포화지방산의 함량은 C처리구에서 가장 낮았으며, A처리구가 가장 높았다(p<0.05). 10주령 계육의 포화지방산의 함량은 5주령 계육과 마찬가지로 C처리구에서 가장 낮았으며, A처리구가 가장 높았다(p<0.05). 계육의 아미노산 함량은 모든 계육의 필수 아미노산 함량은 lysine, leucine, arginine, phenylalanine 등의 순이었고, 비필수 아미노산 함량은 glutamic acid, aspartic acid, alanine 등의 순이었다. 5주령에서는 필수 아미노산 중 valine, leucine, phenylalanine 및 lysine은 A계통에서 가장 낮게 나타났으며, phenylalanine과 lysine 함량은 B계통에서 가장 높았다(p<0.05). 비필수 아미노산 중 cystine, aspartic acid, glycine, alanine 및 proline은 A계통에서 가장 낮았고(p<0.05), 다른 계통들 사이에서는 차이가 없었다. 10주령 계육의 아미노산 함량은 모든 처리구에서 유의적인 차이가 없었다. 계육의 핵산 관련 물질 중 hypoxanthine(Hx)은 5주령에서 C처리구가 14.5%로 가장 높게 나타났으나, 10주령에서는 B처리구가 9.29%로 가장 높게 나타났다(p<0.05). 처리구간 계육의 Inosine monophosphate(IMP)는 각각 131.4 mg/100 g, 187.7 mg/100 g, 250.2 mg/100 g 및 214.0 mg/100 g으로 A, B, C처리구 사이에 유의차가 있었다(p<0.05). 10주령의 IMP는 C처리구가 258.6 mg/100 g으로 가장 높았고(p<0.05), A, B, D처리구 사이에서는 차이가 없었다. 5주령의 adenosine mono phosphate(AMP)는 처리구간 차이가 없었으나, 10주령에서는 5.46 mg/100 g, 5.96 mg/100 g, 5.45 mg/100 g 및 5.47 mg/100 g으로 B처리구에서 가장 높게 나타났다(p<0.05).

• 한국기후변화학회지
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• 제6권4호
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• pp.311-318
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• 2015

Emission characteristics from charbroiling of four different meats (beef, port, duck and chicken) in a pilot-scale cooking facility were investigated in this study. The analyzed air pollutants include gaseous species (CO, NO, $SO_2$, $NH_3$ and PAHs) as well as particulate matters (TSP, $PM_{10}$, $PM_{2.5}$ and black carbon). The emission factors of $PM_{10}$ and PAHs were in the range of 3~47 g-PM/kg-meat and 0.6~11.41 mg-PAHs/kg-meat, respectively, depending on the type of a meat. In addition, the results also revealed that the high ratio of $PM_{2.5}$ to TSP in a meat charbroiling should be considered to design and to operate air pollution control devices.

• 한국동물위생학회지
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• 제31권1호
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• pp.161-166
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• 2008

Deep pectoral myopathy (DPM), also known as Oregon muscle disease or green muscle disease, was first described in 1968 by Dickinson et al as "degenerative myopathy" in turkeys. Even though this condition was first recognized in adult meat-type turkey and chicken breeders, it is becoming more and more common in meat-type growing birds. DPM occurs exclusively in birds that have been specially selected for breast muscle development. It is generally recognized that DPM is an ischemic necrosis that develops in the deep pectoral muscle (supracoracoideus or pectoralis minor muscle) mainly because this muscle is surrounded by inelastic fascia and the sternum, which do not allow the muscle mass to swell in response to the physiological changes occurring when muscle are exercised, as in wing flapping. The lesion does not impair the general health of birds and is generally found during cut-up and deboning, moreover, it can be both unilateral or bilateral, affecting just one or both pectoralis minor muscle, respectively. No public health significance is associated to DPM, but it is aesthetically undesirable. The fillet should be removed, whereas the rest of the carcass is still fit for human consumption. However, the required trimming operations determine the downgrading of the products and produce an economic loss for the industry, especially because it affects the more valuable part of the carcass. The incidence of DPM increases with market weight in broilers, with more cases reported in higher-yielding strains and in males. Increased bird activity (flock nervousness, flightiness, struggle, and wing flapping) induced by factors such as feed or water outages, lighting programs and intensity, human activity, and excessive noises in and around chicken houses should be looked at as a trigger for the development of DPM in broiler. However, most of the studies conducted to evaluate the incidence of DPM in poultry are concerned with parental commercial breeding stocks under experimental conditions (Bianchi et al. 2006. Poult Sci 85 : 1843-1846). There is a possible genetic relationship between the selection for large-breasted birds and this condition. Management procedures that discourage excessive wing flapping would reduce the incidence (Jordan and Pattison. 1998. Poultry diseases. 398-399).

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.526-532
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• 2020

A bacterial strain, designated B301^T and isolated from raw chicken meat obtained from a local market in Korea, was characterized and identified using a polyphasic taxonomic approach. Cells were gram-negative, non-motile, obligate-aerobic coccobacilli that were catalase-positive and oxidase-negative. The optimum growth conditions were 30℃, pH 7.0, and 0% NaCl in tryptic soy broth. Colonies were round, convex, smooth, and cream-colored on tryptic soy agar. Strain B301^T has a genome size of 3,102,684 bp, with 2,840 protein-coding genes and 102 RNA genes. The 16S rRNA gene analysis revealed that strain B301^T belongs to the genus Acinetobacter and shares highest sequence similarity (97.12%) with A. celticus ANC 4603^T and A. sichuanensis WCHAc060041^T. The average nucleotide identity and digital DNA-DNA hybridization values for closely related species were below the cutoff values for species delineation (95-96% and 70%, respectively). The DNA G+C content of strain B301^T was 37.0%. The major respiratory quinone was Q-9, and the cellular fatty acids were primarily summed feature 3 (C_16:1 ω6c/C_16:1 ω7c), C_16:0, and C_18:1 ω9c. The major polar lipids were phosphatidylethanolamine, diphosphatidyl-glycerol, phosphatidylglycerol, and phosphatidyl-serine. The antimicrobial resistance profile of strain B301^T revealed the absence of antibiotic-resistance genes. Susceptibility to a wide range of antimicrobials, including imipenem, minocycline, ampicillin, and tetracycline, was also observed. The results of the phenotypic, chemotaxonomic, and phylogenetic analyses indicate that strain B301^T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter pullorum sp. nov. is proposed. The type strain is B301^T (=KACC 21653^T = JCM 33942^T).

• 한국식생활문화학회지
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• 제11권5호
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• pp.699-710
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• 1996

The sacrificial foods using at 88 villages in Andong area where the village sacrificial rituals are performed, were investigated in 1994. The finding are as follows; 1. Since the village sacrificial rituals keep ancientry, white Baeksulki without any ingredients is used as Ddock (a steamed rice cake) in 74 villages, and in 5 villages the Baeksulki was served just in Siru (a tool used to prepare Ddock). The type of meat serving in rituals has been changing from large animal to small animal and the type of light from bowl-light to candle. 2. Though the ritual observes Confucianism style, memorial address was offered in 34 villages and only in 8 villages, liquor was served 3 times and the address was offered. In 60 villages, liquor was served only once, and calling village god, bowing twice, and burning memorial address paper were performed in order. Burning paper has been meaningful in rituals. 3. Tang (a stew) was served in 21 villages, and in 18 villages even Gook (a soup) was not used. The numbers of village which do not use cooked rice and Ddock are 14, respectively. 4. Fruits are essential in rituals foods. In 63 villages, 3 kinds of fruits are served and this is contrasted with even number of fruits in Bul-Chun-Wi Rituals. 5. In 25 villages, fish and meat are not served. As meat, chicken in 19 village are served. Meat Sikhae (a fermented rice beverage) prepared with meats or fish are served in 5 villages. Slice of dried meat is served in 73 villages commonly. 6. In gender of god for village shrine, woman in 43 villages, couple in 17 villages, man in 9 villages, mountain god in 2 villages, villages tree, etc were enshrined. In 52 villages liquor is served in rituals (liquor is used in 58 villages including serving god), in 28 villages sweet liquor (Gamju) is served for god (sweet liquor is used in 46 villages), and liquor, water, or sweet liquor is just displayed on table but not served in 8 villages. Incensing is found to be not common in village ritual, only in two villages it is performed. The most appropriate rituals food is thought to be pig for mountain god. Home dishes can not be used in village ritual and spoon is not used and chopstick is prepared from wild plant. Meat and fish are used in the raw.

• 대한지역사회영양학회지
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• 제5권4호
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• pp.578-585
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• 2000

This study was aimed at examine eating behavior and food preferences of children in preschool nursery facilities. A survey was conducted on parents using questionnaires including general characteristics, eating behavior, and food habits at home and food preferences of children in 6 nursery facilities. The children involved in this study were 150 boys and 154 girls, aged 1 to 7 years old. Sixty three percent(187 children) of the children were on 'regular' meal times, and 37.0%(110 children) were on 'irregular' meal times. The major reason for their irregular meal times were 'their' concentration on other tasks'(26.6%) and 'no appetite'(25.5%). The meal eaten best by children was 'dinner' while that eaten worst was 'breakfast'. The major type of their eating habit problem was 'unbalanced diet'(62.1%), and their mothers' response to the unbalanced diet was 'trying to persuade the child'(71.6%). The number of snacks at home was one or twice a day(97.3%), and 'preference'(67.8%) was the main reason for selecting particular snacks. Their preferred staple foods were 'cooked rice with meat & vegetables and Chinese noodles', and 'noodles with bean sauce', and their preferred soup was 'seaweed soup' Also, their most preferred meat dishes were 'fried chicken' and 'thin sliced barbecued beef. Children preferred side dishes with animal foods to those with vegetable foods. Since children liked mixed dishes, it seems desirable to introduce foods which children dislike as ingredients in mixed dishes.

• 한국축산식품학회지
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• 제26권3호
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• pp.394-401
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• 2006

Forty Clostridium perfringens isolates were obtained from twelve animal products, following the examination of eighty six beef, pork, broiler chicken and salami meat products, and eleven milk powder products. There were 21 isolates from salami stored at $25^{\circ}C$, 3 isolates from pork, 4 isolates from beef, 9 isolates from broiler chicken, and 3 isolates from milk powder. Only the cpa gene encoding a toxin among the 5 toxin genes tested (cpa, cpb, etx, iap, and cpe) was detected in all forty isolates, suggesting contamination with C. perfringens type A. DNA fingerprinting analysis using PCR of the tRNA intergenic spacer (tDNA-PCR) and the 16S-23S internal transcribed spacer (ITS-PCR), and randomly amplified polymorphic DNA (RAPD) analysis were attempted to differentiate the isolates. RAPD analysis was the most discriminating method among the three PCR analyses. Isolates from the same products tended to show similar RAPD patterns. Antimicrobial susceptibility tests showed that some isolates from broiler chickens had the same antibiogram with multiple resistance to streptomycin, colistin, and ciprofloxacin. Antibiograms were similar between isolates from the same livestock products, but differed considerably between the products.

• 한국환경과학회지
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• 제33권2호
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• pp.179-185
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• 2024

This study aimed to evaluate the differences in canine preferences for pet food terrine according to the processing method. As companion dogs, three male Spitz canines (average age 12 years, average weight 7 kg) and three male Spitz mix canines (average age 12 years, average weight 5 kg) were used in Experiment 1, whereas five male (average age 10 years, average weight 9 kg) and five female Shetland Sheepdogs (Sheltie, average age 10 years, average weight 8 kg) were used in Experiment 2, to evaluate their preferences and intake types. In both experiments, all dog categories mostly preferred 'meat' when it came to their first choice of food consumed, followed by 'vegetables', 'mixed eating', and 'vegetable leftover' last. This confirms means that canines are carnivores, and over time, choosing 'vegetables' or 'vegetable leftovers' as a secondary or alternate food source can be considered a natural process. When provided various terrines, canines in both experiments first ingested 'cooked' rather than 'freeze-dried' duck, chicken, beef, and pork terrines when selecting food at the start of feeding. For both experiments, most of the intake types using duck, chicken, beef, and pork terrine showed a preference for 'after consuming cooked terrine first and then intake freeze-dried' item. In conclusion, the reason for preferring cooked terrine can be attributed to the difference in the preparation cooking method of freeze-dried cooked terrine.

• 한국가금학회지
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• 제40권4호
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• pp.291-297
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• 2013

본 시험은 한국 재래계와 토착종의 2원 교배를 통하여 생산된 실용계 생산성을 연구하고자 수행하였다. 5가지 교배조합으로 생성된 암 수평아리를 각각 30수씩 총 300수를 선별하였으며, 5개의 교배조합은 A) 재래종 R계통 ${\times}$ 토착육용종 S계통, B) 토착겸용종 D계통 ${\times}$ 토착육용종 H계통, C) 토착겸용종 D계통 ${\times}$ 토착육용종 S계통, D) 토착겸용종 C계통 ${\times}$ 재래종 Y계통, E) 재래종 Y계통 ${\times}$ 토착육용종 H계통이다. 교잡종별 증체량은 A와 C 교잡종에서 전반적으로 높은 경향을 나타내었으며, D 교잡종의 증체량은 다른 계통에 비하여 유의적으로 낮은 경향을 보였다(p<0.05). 사료 섭취량의 경우도 증체량과 유사하였으며, 사료 요구율은 전 기간에서 D 교잡종이 유의적으로 높은 경향을 나타내었다(p<0.05). 도체중 및 도체율은 D 교잡종이 다른 계통에 비하여 유의적으로 낮았으며, C 교잡종의 도체중이 7주령과 10주령에서 가장 높았으며, 13주령에서는 A 교잡종이 우수한 경향을 나타내었다(p<0.05). 부분육 생산 비율은 다리 30.60~33.75%, 가슴 21.48~24.72%, 날개 11.95~14.33%, 등 21.91~25.83%, 목 7.38~8.72%로 다리의 부분육 생산 비율이 가장 높게 나타났다. 이러한 연구 결과를 통하여 생성된 5교잡종을 3원교잡이나 4원교잡종 생산의 부계나 모계로 사용하여 산육성과 산란성이 우수한 토종 실용계를 생산하는데 기초자료로 이용될 것이라 사료된다.