• Food Science and Preservation
• /
• v.22 no.4
• /
• pp.553-558
• /
• 2015

The aims of this study was to optimize the extraction conditions of sinigrin from Dolsan leaf mustard. Dolsan leaf mustard (Dolsan-eup, Yeosu-si) harvested during at May 2014 was used for sinigrin extraction. After the extraction of sinigrin using 50% $CH_3CN$, 10% $NH_4Cl$, 60% $CH_2OH$, and 70% $CH_3OH$, the sinigrin content was measured by HPLC analysis. The results showed that sinigrin content was highest with 50% $CH_3CN$ solvent extraction and UV detector sensitivity was greater at 228 nm rather than at 242 nm. The sinigrin concentrations of leaf, stem and root with 50% $CH_3CN$ extraction were 345 ppm, 728 ppm, and 539 ppm, respectively. After extraction of the different parts of Dolsan leaf mustard, The standard retention time by HPLC analysis of sinigrin content was 2.054, 2.032, 2.059, and 2.035 min from the root, stalk, and leaf, respectively. On the other hand, HPLC analysis showed that the leaf extracts contained glucoraphanin, one of glucosinolates. The optimum time and extraction solvent for the sinigrin extraction from Dolsan leaf mustard was found to be 24 hr with 50% $CH_3CN$ solvent. In addition, opotimum UV detector k at 228 nm. These results showed that the optimum extraction conditions for Dolsan leaf mustard were 24 hr extraction with 50% $CH_3CN$ solvent. In addition, the optimum wavelength of UV detector was determined to be 228 nm for sinigrin analysis. Therefore, this study could provide a useful information for sinigrin extraction and its systematic analysis during the storage.

• The Korean Journal of Malacology
• /
• v.29 no.2
• /
• pp.105-111
• /
• 2013

The gonadal development of triploid and diploid Pacific abalones, Haliotis discus hannai was histologically investigated in spawning season. Diploid abalones had matured oocytes and spermatozoa, but most triploid had spermatocytes or developing oocytes that was slightly retarded in gonadal development compared to diploid abalones. In spawning experiment of triploid and diploid abalones, spawning rates of diploid male and female were 100%, but those of triploid female was 50% and male was 25% respectively. Investigation of spawned abalone eggs and spermatozoa revealed that length of diploid sperms head were 17.47 ${\mu}m$, breadth of head were 10.31 ${\mu}m$, length of spermatozoa were 130.72 ${\mu}m$, but those of triploid spermatozoa were 11.83 ${\mu}m$, 7.89 ${\mu}m$ and 103.36 ${\mu}m$ respectively. Triploid spermatozoa were significantly small to diploid spermatozoa (p < 0.05). The eggs of diploid and triploid were not different in size. The cross experiment between oocytes produced by triploids and spermatozoa by diploids ($3n{\times}2n$ cross) revealed that no fertilization were occurred, and $2n{\times}3n$ cross also revealed same result.

• Development and Reproduction
• /
• v.16 no.2
• /
• pp.77-85
• /
• 2012

Various cryoprotective agents (CPA) were tested to establish the best conditions for the cryopreservation of sperm from black porgy Acanthopagrus schlegeli acclimated and raised in freshwater (BFW). Survival rates of frozen/thawed sperm from BFW were higher in the order of dimethy sulfoxide (DMSO), glycerol, ethylene glycol (EG) and methanol. Sperm motility was higher in the order of glycerol, DMSO, EG and methanol. These effects were the same in thawed sperm from black porgy raised in seawater (BSW). Thus, optimum CPA for sperm cryopreservation of BFW and BSW were DMSO and glycerol where the highest survival rates and sperm motility were found at the concentration of 10%. In particular, the survival rates and motility of thawed sperm from BFW and BSW after cryopreservation using 10% DMSO were better than when cryopreserved using 10% glycerol. On the other hand, for the thawed sperm from both BFW and BSW, the longer the preservation period was, the lower the survival rates and sperm motility were. Notably, the higher the concentration of CPA was, the lower the survival rates and sperm motility were.

• Journal of the Korean Applied Science and Technology
• /
• v.35 no.2
• /
• pp.485-491
• /
• 2018

The purpose of this study was to probe the influences of krill (Euphausia superba) meal supplementation on a dose effect relationship between fluoride levels of krill meal and serum hepatic functional enzyme activity such as alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) in rats fed experimental diets for 5 weeks. There were no significant differences in the activities of ALP, AST, ALT, and LDH in sera among krill meal diet groups (KM10, KM20, KM30). However, these groups were significantly (p<0.05) lower enzyme activities than control group (CG). The fluoride levels of sera and organ tissues (liver, brain, heart, lung, kidney) in krill meal diet groups (KM10, KM20, KM30) were significantly increased by adding krill meal in comparison with CG. The results indicate that a difficult to found toxicity to the liver from krill meal diet groups.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.43 no.6
• /
• pp.715-722
• /
• 2010

The effects of temperature, salinity and irradiance on the growth of the harmful red tide dinoflagellate Cochlodinium polykrikoides Margelef isolated from the South Sea of Korea were examined in the laboratory. Growth was examined under the following combinations of temperature and salinity: 15, 20, 25 and $30^{\circ}C$, and 15, 20, 25, 30 and 35 psu at a constant irradiance of $180\;{\mu}mol/m^2/s$. No growth was observed with a temperature of $15^{\circ}C$ and a salinitiy of 15 psu. Moderate growth rates of more than 0.30 /day were obtained at $25^{\circ}C$ with salinities of 25.35 psu. These values are similar to in situ observations for this species. The maximum growth rate, 0.35 /day, was obtained at $25^{\circ}C$ and 30 psu. In light experiments, cell growth of C. polykrikoides was conducted with constant temperature ($20^{\circ}C$) and salinity (30 psu) under light photon flux densities (PFD) of 10, 25, 50, 70, 100, 150, 250 and $350\;{\mu}mol/m^2/s$. C. polykrikoides did not grow at $10\;{\mu}mol/m^2/s$. Cell growth was observed at irradiance values of $25\;{\mu}mol/m^2/s$ and above. The irradiance-growth curve was described as ${\mu}=0.30{\cdot}(I-15.27)/(I+27.22)$, (r=0.99). This suggests a compensation PFD of $15.27\;{\mu}mol/m^2/s$ and a maximum growth rate of 0.30 /day. In conclusion, C. polykrikoides prefers high salinity, temperature and irradiance in summer in Korea. These results provide important information for understanding the mechanism of C. polykrikoides blooms and developing technology to predict blooms of this organism in the field.

• Korean Journal of Remote Sensing
• /
• v.31 no.5
• /
• pp.473-490
• /
• 2015

There are diverse sea areas within the coverage of GOCI which is observed around the Korea at one-hour intervals. It includes not only very clear ocean of East Sea, but also extremely turbid waters of the Yangtze River estuary. In this study, we analyzed the different optical characteristics of various sea areas using absorption coefficients of phytoplankton, Suspended Particulate Matter(SPM), Dissolved Organic Matter(DOM). Totally 959 sets of bio-optical and marine environmental data were obtained from 2009 to 2014 around the sea area of Korea. The East Sea, South Sea, East China Sea and offshore part of Yellow Sea showed similar pattern having high levels of contribution of phytoplankton and DOM. On the other hands, the coastal part of Mokpo and Gyeonggi Bay showed opposite pattern having high levels of contribution of SPM and DOM. As a result of the algorithm performance for chlorophyll-a(Chl-a) and SPM, Chl-a is mostly overestimated and SPM is mainly tended to be underestimated. Large amount of errors are induced by the SPM rather than the chl-a and DOM. These errors are primarily founded in the coastal waters having relatively high levels of $a_{SPM}$ contribution of more than 60%.

• Journal of fish pathology
• /
• v.22 no.1
• /
• pp.67-73
• /
• 2009

It was examined whether the common belief that "cultured puffer fishes do not contain tetrodotoxin (TTX)", the major lethal substance that accidently causes death in consumers of those fishes, is true in river puffer fish Takifugu obscurus. In mouse bioassay, lethal levels of toxins were detected in the ranks: gonad>liver>intestine>muscle>skin in wild puffer fish. In contrast, no mortality occurred in the mouse bioassay on cultured fish. However, there were sleepiness, sluggish behavior, and hind limb paralysis with the tissue extracts of cultured fish suggesting the presence of TTX or other similarly acting toxins. An attempt to confirm the presence of TTX in cultured fish with high performance liquid chromatography (HPLC) was not very successful. The results suggest possible existence of TTX toxins or similarly acting toxins.

• Journal of Life Science
• /
• v.21 no.11
• /
• pp.1599-1606
• /
• 2011

To evaluate the effects of microorganism agents on oil biodegradation, treatability and microcosm studies were conducted. Petroleum oil degrading bacteria were isolated from enriched cultures of oil-contaminated sediment samples using a mineral salts medium (MSM) containing 0.5% Arabian heavy crude oil as the sole carbon source. After a 5 day-incubation period using MSM, mixed microorganisms of three species (strains BS1, BS2 and BS4) degraded 48.4% of aliphatic hydrocarbons and 30.5% of aromatic hydrocarbons. Treatability and microcosm tests were performed in the three different treatment conditions (AO: Arabian heavy crude oil, AO+IN: Arabian heavy crude oil+inorganic nutrient, AO+IN+MM: Arabian heavy crude oil+inorganic nutrient+mixed microorganism agents). Among these, significantly enhanced biodegradation of aliphatic hydrocarbons were observed in AO+IN and AO+IN+MM conditions, without showing any different biodegradation rates in either condition. However, the degradation rates of aromatic hydrocarbons in an AO+IN+MM condition were increased by 50% in the treatability test and by 13% in the microcosm test compared to those in an AO+IN condition. Taken together, it can be concluded that mixed microorganism agents enhance the biodegradation of aliphatic and aromatic hydrocarbons in laboratory, a treatability test, and a microcosm test. This agent could especially be a useful tool in the application of bioremediation for removal of aromatic hydrocarbons.

• Journal of Life Science
• /
• v.27 no.7
• /
• pp.834-839
• /
• 2017

Propolis is a natural product collected from plants by honey bees product used extensively in traditional medicine for its antioxidant, anti-inflammatory, immunomodulatory and anti-cancer effects. Propolis exhibits a broad spectrum of biological activities because it is a complex mixture of natural substances. Ovarian cancer is the second most common newly diagnosed cancer from all cancers among women in Korea and the leading cause of death from gynecological malignancies. While most ovarian cancer patients initially respond to surgical debulking and chemotherapy, patients later succumb to the disease. Thus, there is an urgent need to test novel therapeutic agents to counteract the high mortality rate associated with ovarian cancer. In this study, we investigated the anti-cancer properties and the active mechanism of Australian propolis in human epithelial ovarian cancer A2780 cells. Our data revealed that propolis showed a cytotoxic activity in a dose-dependent manner. Flow cytometric analysis for cell cycle arrest and apoptosis using propidium iodide staning and annexin V-FITC indicated that propolis could induce cycle arrest in the G0/G1 phase and apoptosis in a dose-dependent manner on human epithelial ovarian cancer cells. These results suggest that the Australian propolis is potential alternative agent on ovarian cancer prevention and treatment.

• KSBB Journal
• /
• v.24 no.3
• /
• pp.321-326
• /
• 2009

This study was to optimize the medium components for astaxanthin production in Paracoccus sp. through surface response methodology. A screening test was first conducted on 5 medium components using a Plackett-Burman design, from which $MgSO_4$ and yeast extract were identified as the significant factors affecting astaxanthin production. These significant factors were optimized by central composite design of experiments and response surface methodology, as 2.83 g/L $MgSO_4$ and 7.02 g/L yeast extract, respectively. The expected astaxanthin concentration with these optimized medium compositions were 0.925 mg/L. In flask culture, the experimentally obtained concentration of astaxantin was 1.021 mg/L, where it had been 0.4 mg/L before optimization.