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http://dx.doi.org/10.5352/JLS.2011.21.11.1599

Evaluation of Petroleum Oil Degrading Mixed Microorganism Agent for the Bioremediation of Petroleum Oil Spilled in Marine Environments  

Sohn, Jae-Hak (Department of Bio-food material, College of Medical & Life Sciences, Silla University)
Publication Information
Journal of Life Science / v.21, no.11, 2011 , pp. 1599-1606 More about this Journal
Abstract
To evaluate the effects of microorganism agents on oil biodegradation, treatability and microcosm studies were conducted. Petroleum oil degrading bacteria were isolated from enriched cultures of oil-contaminated sediment samples using a mineral salts medium (MSM) containing 0.5% Arabian heavy crude oil as the sole carbon source. After a 5 day-incubation period using MSM, mixed microorganisms of three species (strains BS1, BS2 and BS4) degraded 48.4% of aliphatic hydrocarbons and 30.5% of aromatic hydrocarbons. Treatability and microcosm tests were performed in the three different treatment conditions (AO: Arabian heavy crude oil, AO+IN: Arabian heavy crude oil+inorganic nutrient, AO+IN+MM: Arabian heavy crude oil+inorganic nutrient+mixed microorganism agents). Among these, significantly enhanced biodegradation of aliphatic hydrocarbons were observed in AO+IN and AO+IN+MM conditions, without showing any different biodegradation rates in either condition. However, the degradation rates of aromatic hydrocarbons in an AO+IN+MM condition were increased by 50% in the treatability test and by 13% in the microcosm test compared to those in an AO+IN condition. Taken together, it can be concluded that mixed microorganism agents enhance the biodegradation of aliphatic and aromatic hydrocarbons in laboratory, a treatability test, and a microcosm test. This agent could especially be a useful tool in the application of bioremediation for removal of aromatic hydrocarbons.
Keywords
Bioremediation; microcosm test; petroleum degrading microorganism; petroleum hydrocarbon; treatability test;
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1 Zimmerman, R. 1977. Estimation of bacterial number and biomass by epifluorescence microscopy and scanning electron microscopy, pp. 103-120, In Rheinheimer, G. (ed.), Microbial ecology of brackish water environment. Springer-Verlag, Berlin.
2 Kim, S. J., J. H. Sohn, D. S. Sim, K. K. Kwon, and T. H. Kim. 1998. The effects of bioremediation on the oil bioremediation in oil polluted environments, pp. 181-188, In Le Gal, Y. and H.O. Halvorson (eds.), New developments in marine biotechnology, plenum Press. New York.
3 Leahy, J. G. and R. R. Colwell. 1990. Microbial degradation of hydrocarbons in the environment. Microbial Rev. 53, 305-315.
4 Lee, K. and E. M. Levy. 1987. Enhanced biodegradation of a light crude oil in sandy beaches. Proceedings of the 1987 Oil Spill Conference. American Petroleum Institute. Washington. DC.
5 Lee, K. and E. M. Levy. 1989. Enhancement of the natural biodegradation of condensate and crude oil on beaches of Altantic Canada. Proceedings of the 1989 Oil Spill Conference. American Petroleum Institute. Washington. DC.
6 Lee, K., G. H. Tremblay, and E. M. Levy. 1993. Bioremediation: Application of slow-release fertilizers on low-energy shoreline. Proceedings of 1993 International Oil Spill Conference, American Petroleum Institute. Washington DC., USA.
7 Marty, P. and Y. Martin. 1996. Seed and feed strategy against oil spills in a marine environment: laboratory and simulated outdoor experiments with selected natural bacterial strain. J. Mar. Biotechnol. 4, 155-158.
8 Oh, Y. S., D. S. Sim, and S. J. Kim. 2001. Effects of nutrients on crude oil biodegradation in the upper intertidal zone. Mar. Pollut. Bull. 42, 1367-1372.   DOI   ScienceOn
9 Ortega-Calvo, J. J. and C. Saiz-Jimenez. 1998. Effect of humic fractions and clay on biodegradation of phenanthrene by a Pseudomonas fluorescens strain isolated from soil. Appl. Environ. Microbiol. 64, 3123-3126.
10 Rosenberge, E., R. Legmann, A. Kushmaro, R. Taube, E. Adler, and E. Z. Ron. 1992. Petroleum bioremediation- A multiphase problem. Biodegradation 3, 337-350.   DOI   ScienceOn
11 Safferman, S. I. 1991. Selection of nutrients to enhance biodegradation for the remediation of oil spilled on beaches. Proceedings of 1991 International Oil Spill Conference, American Petroleum Institute. Washington DC..
12 Boopathy, R. 2000. Factors limiting bioremediation technologies. Bioresource Technology 74, 63-67.   DOI   ScienceOn
13 Aldrett, S., J. S. Bonner, T. J. McDonalds, M. A. Mills, and R. L. Autenrieth. 1997. Degradation of crude oil enhanced by commercial microbial cultures. Proceedings of the 1997. Oil Spill Conference. American Petroleum Institute. Washington. DC.
14 Alexander, M. 1994. Biodegradation and Bioremediation. Academic Press, San Diego.
15 Altas, R. M. 1981. Microbial degradation of petroleum hydrocarbon: An environmental perspective. Microbial Rev. 45, 120-209.
16 Choi, S. C., K. K. Kwon, J. H. Sohn, and S. J. Kim. 2002. Evaluation of fertilizer additions to stimulate oil bioremediation in sand seashore mesocosms. J. Microbiol. Biotechnol. 12, 431-436.
17 Forsyth., J. V., Y. M. Tsao, and R. D. Blem. 1995. Bioremediation: when is augmentation needed, pp. 1-44, In Hinchee, R.E. et al. (eds.), Bioaugmentation for site remediation. Battelle Press, Columbus, OH.
18 Tagger, S., A. Bianchi, M. Juillard, J. LePetit, and B. Roux. 1983. Effect of microbial seeding of crude oil in seawater in a model system. Mar. Biol. 78, 13-20.   DOI   ScienceOn
19 Sim, D. S., J. H. Sohn, and S. J. Kim. 1998. Microcosm study for bioremediation of oil-contaminated pebble environments. Korean J. Microbial. 34, 101-107.
20 Sohn, J. H., K. K. Kwon, and S. J. Kim. 2003. Effects of Slow Release Fertilizer and Dispersant on the Biodegradation of oil contaminated in sand seashore mesocosm. Korean J. Microbiol. 39, 8-15.
21 Vacca, D. J., W. F. Bleam, and W. J. Hickey. 2005. Isolation of soil bacteria adapted to degrade humic acid-sorbed phenanthrene. Appl. Environ. Microbiol. 71, 3797-3805.   DOI   ScienceOn
22 Venosa, A. D., J. R. Haines, W. Nisamaneepong, R. Govind, S. Pradhan, and B. Siddique. 1991. Protocol for testing bioremediation products against weathered Alaskan crude oil. Proceedings of the 1991 Oil Spill Conference. American Petroleum Institute. Washington. DC.
23 Venosa, A. D., M. T. Suidan, B. A. Wrenn, K. L., Strohmeier, J. R. Haines, A. L, Eberhart, D. King, and E. Holder. 1996. Bioremediation of an experimental oil spill on the shoreline of Delaware Bay. Environ. Sci. Technol. 30, 1764-1775.   DOI   ScienceOn
24 Glaser, J. A., A. D. Venisa, and E. J. Opatken. 1991. Development and evolution of application techniques for delivery of nutrients to contamined shoreline in Prince William Sound. Proceedings of 1989 International Oil Spill Conference. American Petroleum Institute. Washington DC., USA.
25 Head, L. M. and R. P. J. Swannell. 1999. Bioremediation of petroleum hydrocarbon contaminants in marine habitats. Curr. Opin. Biotechnol. 10, 234-239.   DOI   ScienceOn
26 Kim, S. J. 2002. Research for the form approval procedure of oil spill bioremediation agent. Korea Coast Guard