Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Possible existence of tetrodotoxin-like toxins in cultured river puffer fish, Takifugu obscurus

양식산 황복에서 tetrodotoxin 유사 독소의 미량 존재 가능성 제시

  • Kim, Do-Young (Bio-Evaluation Center, KIRBB) ;
  • Kim, Ju-Wan (Wando Maritime Office, Mokpo Regional Maritime Affairs and Port Office) ;
  • Park, Ki-Seok (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Kang, Hee-Woong (West Sea Fisheries Research and Development Institute, NFRDI) ;
  • Jeon, Joong-Kyun (Division of Marine Bioscience and Technology, College of Life Sciences, Kangnung National University) ;
  • Chung, Joon-Ki (Department of Aquatic Life Medicine, College of Fisheries Sciences, Pukyung National University) ;
  • Choi, Sang-Hoon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Choi, Min-Soon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Park, Kwan-Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 김도영 (한국생명공학연구원 바이오평가센터) ;
  • 김주완 (국토해양부 목포지방해양항만청 완도사무소) ;
  • 박기석 (군산대학교 해양과학대학 수산생명의학과) ;
  • 강희웅 (국립수산과학원 서해수산연구소) ;
  • 전중균 (강릉대학교 생명과학대학 해양생명공학부) ;
  • 정준기 (부경대학교 수산과학대학 수산생명의학과) ;
  • 최상훈 (군산대학교 해양과학대학 수산생명의학과) ;
  • 최민순 (군산대학교 해양과학대학 수산생명의학과) ;
  • 박관하 (군산대학교 해양과학대학 수산생명의학과)
  • Published : 2009.04.01
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Abstract

It was examined whether the common belief that "cultured puffer fishes do not contain tetrodotoxin (TTX)", the major lethal substance that accidently causes death in consumers of those fishes, is true in river puffer fish Takifugu obscurus. In mouse bioassay, lethal levels of toxins were detected in the ranks: gonad>liver>intestine>muscle>skin in wild puffer fish. In contrast, no mortality occurred in the mouse bioassay on cultured fish. However, there were sleepiness, sluggish behavior, and hind limb paralysis with the tissue extracts of cultured fish suggesting the presence of TTX or other similarly acting toxins. An attempt to confirm the presence of TTX in cultured fish with high performance liquid chromatography (HPLC) was not very successful. The results suggest possible existence of TTX toxins or similarly acting toxins.

양식산 복어에서는 tetrodotoxin(TTX)가 존재하지 않는다고 일반적으로 생각되고 있다. 본 연구에서는 실제로 양식산 황복(Takifugu obscurus)에는 TTX가 존재하지 않는지 확인하기 위해 생쥐를 이용한 bioassay로 여러 장기를 동시에 분석하였다. 자연산에서는 동물이 치사하는 수준의 독력을 함유한 시료가 있었으며 정소>간장>장>근육>피부의 순으로 독력이 확인되었다.양식산 시료에서는 TTX의 미량존재로 추정되는 졸림, 행동둔화, 후지마비 등의 증상이 나타났지만 치사동물은 발견되지 않았다. 이 결과는 양식 산 황복에서 TTX가 매우 낮은 농도로 존재할가능성을 제시하지만 기기분석법을 이용한 정확한 정량이 필요하다고 판단된다.

Keywords

References

  1. 수산식품분석지침서: 국립수산물품질검사원, pp. 3042-354, 1998
  2. 김경환: 이우주의약리학강의. 제 5판, 의학문학사, 1997
  3. 김경찬, 박진우, 이명자, 김상록, 김동수, 김현대, 박영호: 시판되고 있는 까칠복(Fugu stictonotus)의 독성. Bull. Kor. Fish, Soc., 28:31-34, 1995
  4. 김동수, 김삼록, 이명지, 설명훈, 정동윤, 김현대: 중국으로부터 수입된 삼채복(Fugu flavidus)의 독성. J. Kor. Fish. Soc,. 28:533-538, 1995
  5. 안석근, 임용수, 김재광, 민순식, 류 일, 양혁준, 박철완, 이 근: 복어 중독의 임상적 고찰. 대한응급의학회지, 10:447-455, 1999
  6. 전중균, 유재명: 한국산 복어의 독성Ⅰ: 황복의 부위별 독성. J. Kor. Fish. Soc., 28:137-140, 1995
  7. 衛生試驗法.註解: 日本藥學會編, 山根靖弘編輯, 金原出版株式會社, 1995
  8. Jang, J. and Yotsu-Yamashita, M.: Distribution of tetrodotoxin, saxitoxin, and their analogs among tissues of the puffer fish Fugu pardalis. Toxicon, 48: 980-987, 2006 https://doi.org/10.1016/j.toxicon.2006.07.034
  9. Kungsuwan, A., Arakawa. O,, Promdet, M. and Onoue, Y.: Occurrence of paralytic shellfish poisons in Thai freshwater puffers. Toxicon, 35:1341-1346, 1997 https://doi.org/10.1016/S0041-0101(97)00001-9
  10. Matsui, T., Sato, H., Hamada, S. and Shimizu, C.: Comparison of toxicity of the cultured and wild puffer fish Fugu niphobles. Bull. Jpn. Soc. Sci. Fish., 48:253, 1982 https://doi.org/10.2331/suisan.48.253
  11. Matsumura, K.: A monoclonal antibody against tetrodotoxin that reacts to the active group for the toxicity. Eur. J. Pharmacol., 293:41-45, 1995 https://doi.org/10.1016/0014-2999(95)90112-4
  12. Matsumura, K.: Tetrodotoxin concentrations in cultured puffer fish, Fugus rubripes. J. Agr. Food Chem., 44:1-2, 1996 https://doi.org/10.1021/jf950576l
  13. Matsumura, K.: Production of tetrodotoxin in puffer fish embryo. Environ. Toxicol. Pharmacol., 6:217-219, 1998 https://doi.org/10.1016/S1382-6689(98)00037-4
  14. Nagashima, Y., Hamada, Y., Ushio, H., Nishio, S., Shimakura, K. and Shiomi, K.: Subcellular distribution of tetrodotoxin in puffer fish liver. Toxicon, 37:1833-1837, 1999 https://doi.org/10.1016/S0041-0101(99)00100-2
  15. Noguchi, T., Jeon, J.K., Arakawa, O., Sugita, H., Deguchi, Y., Shida, Y. and Hashimoto, K.: Occurrence of tetrodotoxin and anhydrotetrodotoxin in Vibrio sp. isolated from the intestine of a xanthid crab, Atergatis floridus. J. Biochem., 99:311-314, 1986 https://doi.org/10.1093/oxfordjournals.jbchem.a135476
  16. Nu$\check{n}$ez-V$\acute{a}$zquez, E.J., Yotsu-Yamashita, M., Sierra-Beltr$\acute{a}$n, A.P., Yasumoto, T. and Ochoa, J.L.: Toxicities and distribution of tetrodotoxin in the tissues of puffer fish found in the coast of the Baja California Peninsula, Mexico. Toxicon, 38:729-734, 2000 https://doi.org/10.1016/S0041-0101(99)00189-0
  17. Ritchie, K.B., Nagelkerten, I., James, S. and Smith, G.W.: A tetrodotoxin-producing marine pathogen. Nature, 404:354, 2000 https://doi.org/10.1038/35006168
  18. Saito, T., Maruyama, J., Kanoh, S., Jeon, J.K., Noguchi, T., Harada, T. and Murata, O.: Toxicity of the cultured Pufferfish Fugu rubripes along with their resistibility aganist tetrodotoxin. Bull. Jpn Soc. Sic. Fish., 50:1573-1575, 1984 https://doi.org/10.2331/suisan.50.1573
  19. Tani, I.: Toxicological studies of puffers in Japan. Teikokutosho, Tokyo, pp. 55-57, 1945
  20. Yasumoto, T., Yasumura, D., Yotsu, M., Michishita, T., Endo, A. and Kitaoki, Y.: Bacterial production of tetrodotoxin and anhydrotetrodotoxin. Agr. Biol. Chem., 50:793-795, 1986 https://doi.org/10.1271/bbb1961.50.793
  21. Yotsu-Yamashita, M., Sugimoto, A., Terakawa, T., Shoji, Y., Miyazawa, T. and Yasumoto, T.: Purification, characterization, and cDNA cloning of a novel soluble saxitoxin and tetrodotoxin binding protein from plasma of the puffer fish, Fugus pardalis. Eur. J. Biochem., 268:5937-5946, 2001 https://doi.org/10.1046/j.0014-2956.2001.02547.x